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Abundance and diversity of ammonia-oxidizing archaea in response to various habitats in Pearl River Delta of China, a subtropical maritime zone

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Journal of Environmental Sciences 2013年第6期25卷 1195-1205页

作者： Zhixin Li wenbiao jin Zhaoyun Liang Yangyang Yue Junhong Lv Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control Harbin Institute of Technology Shenzhen Graduate School Shenzhen Institute of Advanced Technology Chinese Academy of Sciences

Ammonia-oxidizing archaea （AOA） are widely considered key to ammonia oxidation in various environments. However, little work has been conducted to simultaneously investigate the abundance and diversity of AOA as well as correlations between archaeal amoA genotypes and environmental parameters of different ecosystems at one district. To understand the abundance, diversity, and distribution of AOA in Pearl River Delta of China in response to various habitats, the archaeal amoA genes in soil, marine, river, lake, hot spring and wastewater treatment plant （WWTP） samples were investigated using real-time fluorescent quantitative PCR and clone libraries. Our analyses indicated that the diversity of AOA in various habitats was different and could be clustered into five major clades, i.e., estuary sediment, marine water/sediment, soil, hot spring and Cluster 1. Phylogenetic analyses revealed that the structure of AOA communities in similar ecological habitats exhibited strong relation. The canonical correspondence method indicated that the AOA community structure was strongly correlated to temperature, pH, total organic carbon, total nitrogen and dissolved oxygen variables. Assessing AOA amoA gene copy numbers, ranging from 6.84× 10^6 to 9.45 × 10^7 copies/g in dry soil/sediment, and 6.06× 10^6 to 2.41 ×10^7 copies/L in water samples, were higher than ammonia-oxidizing bacteria （AOB） by 1-2 orders of magnitude. However, AOA amoA copy numbers were much lower than AOB in WWTP activated sludge samples. Overall, these studies suggested that AOA may be a major contributor to ammonia oxidation in natural habitats but play a minor role in highly aerated activated sludge. The result also showed the ratio of AOA to AOB amoA gene abundance was positively correlated with temperature and less correlated with other environmental parameters. New data from our study provide increasing evidence for the relative abundance and diversity of ammonia-oxidizing archaea in the global nitrogen cyc

关键词： ammonia-oxidizing archaea abundance community structure real-time quantitative PCR clone library Pearl River Delta

Mechanism on minimization of excess sludge in oxic-settling- anaerobic (OSA) process

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Frontiers of Environmental Science & Engineering 2008年第1期2卷 36-43页

作者： Jianfang WANG Qingliang ZHAO wenbiao jin Jikan LIN School of Municipal&Environmental Engineering Harbin Institute of TechnologyHarbin 150090China Harbin Institute of Technology Campus of Shenzhen University Town Shenzhen 518055China Bio-treat Technology Limited Dongguan 523581China

The oxic-settling-anaerobic(OSA)process is a promising wastewater treatment technique for efficiently reducing sludge production and improving the stability of process *** this paper,the possible factors of sludge reduction such as sludge decay,uncoupled metabol-ism,and anaerobic oxidation with low sludge production were discussed in the OSA *** has been confirmed that sludge decay is the decisive cause in the OSA process,accounting for 66.7%of sludge production *** decay includes hydrolysis and acidogenesis of dead microorganisms and particle organic carbon adsorbed in sludge floc and endogenous *** batch experi-ments,it has been proven that there is energetic uncoupling in the OSA system since microorganisms were exposed to alternative anaerobic and aerobic *** accounts for about 7.5%of sludge production *** chemical oxygen demand(SCOD)released from the anaerobic sludge tank in the OSA process was used as the substrate for cryptic *** substrate was used for anoxic denitrifying,anaerobic phosphorus release,sulfate reduction,and methane *** anaerobic reactions in the sludge anaerobic tank have lower sludge production than in the aerobic oxidation when equivalent SCOD is consumed,which may lead to approximately 23%of sludge reduction in the OSA *** has been concluded that multiple causes resulted in the minimization of excess sludge in the OSA *** microbial community structure and diversity of sludge samples from the CAS(conventional activated sludge)and OSA systems were investigated by 16 SrDNA PCR-DG-DGGE(poly-merase chain reaction-double gradient-denaturing gradient gel electrophoresis).DGGE profile and cluster analysis showed more abundant species in the OSA system contrasting to microbial communities in the CAS system.

关键词： sludge minimization sludge decay uncoupled metabolism denaturing gradient gel electrophoresis(DGGE) microbial community

Tuning interfacial charge transfer for efficient visible-light-driven photodegradation and simultaneous H_(2)evolution

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Journal of Materials Science & Technology 2024年第1期168卷 35-49页

作者： Zhiyang Li Yaogang Chen Yinghe Zhang Wei Ai Qian Lei Tingjun Yao Dan Zhong Wenjie Liu wenbiao jin Lei Yang State Key Laboratory of Urban Water Resource and Environment Shenzhen Key Laboratory of Organic Pollution Prevention and ControlSchool of Civil and Environmental EngineeringHarbin Institute of Technology ShenzhenShenzhen 518055China Key Laboratory of Northwest Water Resources Environment and EcologyMinistry of EducationSchool of Environmental and Municipal EngineeringXi’an University of Architecture and TechnologyXi’an 710055China CNAS Accredited Test Center Guangdong Engineering Technology Research Center of Air PurificationHealthlead Co.Ltd.Shenzhen 518055China School of Science Harbin Institute of Technology ShenzhenShenzhen 518055China

The edge-graphitized carbon nitride(C_(3)N_(4)-C g)was prepared by secondary pyrolysis to construct ZnO/C_(3)N_(4)-C g(ZCN)type-Ⅱheterojunction photocatalyst via a facile sonication dispersion method,which achieved∼7.04-fold and∼18.3-fold enhanced visible-light-driven photocatalytic performance for refrac-tory micropollutant removal and simultaneous hydrogen(H_(2))evolution respectively compared to con-ventional ZnO/g-C_(3)N_(4)Step-scheme *** apparent quantum efficiency of the ZCN_(0.4)het-erojunction reaches 0.92%(λ=420 nm).Such excellent performance stems from that the edge-graphene moieties stitched onto the interface of heterojunction extend light absorption to the full visible spec-trum,meanwhile,the built-in electric field generated during Fermi level alignment accompanying fa-vorable band-bending structure provides an effective pathway for the rapid migration of photoinduced electrons via the edge graphene channel to improve interfacial charge separation ***,the midgap states introduced in ZCN heterojunction could temporarily retain photoexcited electrons to effectively inhibit the in situ carrier recombination for improved photocatalytic H_(2)***,ZCN/peroxymonosulfate system exhibited excellent anti-interference performance against complex water bodies under visible illumination due to the synergistic effect between the co-existing anions and organic ***,the eco-friendly nature of the ZCN/peroxymonosulfate system showed no biotoxicity of reaction filtrate on cell proliferation after treatment,which avoided secondary ***-ering the outstanding performance in photocatalysis,the ZCN system exhibits broad potential for practical applications in water pollution control and green energy production.

关键词： ZnO C_(3)N_(4)-C g Heterojunction Photodegradation Hydrogen evolution

Directional Breeding of High Itaconic Acid Yielding Strain of <i>Aspergillus terreus</i>with a New Plate Technique

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Advances in Microbiology 2013年第4期3卷 376-381页

作者： Lingyun Wei Jianhua Wang Hezhi Zhou wenbiao jin Zhangli Hu Jiazuan Ni Key Laboratory for Green Chemical Process of Ministry of Education Wuhan Institute of Technology Wuhan China

Itaconic acid is commercially produced by the cultivation of Aspergillus terreus using starch hydrolysate as carbon source. The degree of hydrolysis had a great influence on itaconic acid production which was suitable when corn starch was saccharified at 35 DE. The α-amylase was sufficient to drive the starch hydrolysis to the degree. The agar plate assay with LiCl treatment provided a rapid, simple and unequivocal method for screening large numbers of colonies for itaconic acid producing strains. It was learned by experience that the strains on the plates with thick hyphae and light-colored spores often accompanied high itaconic acid production. A strain, designated Ast165, producing itaconic acid with a high yield, was successfully obtained by directional breeding of metabolic end products resistant strains. The itaconic acid concentration produced by Ast165 was 53.8 g/l from 100 g/l of starch hydrolysate in shake flasks. The conversion rate was 61.3%, which was the highest value found in tests.

关键词： Aspergillus terreus Itaconic Acid Corn Starch Dextrose Equivalent Enzymatic Hydrolysis

基于IFS分形插值的序列切片数据三维重构算法

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计算机工程 2007年第12期33卷 228-230页

作者：王梦金文标重庆邮电大学计算机学院重庆400065

提出了一种三维重构算法,采用分形插值方法对三维物体多个序列切片的特征数据点进行插值重构。传统的三维迭代函数系统(IFS)分形插值算法只适用于矩形剖分,而序列切片的特征数据形成的网格往往是不规则的四边形网格。该文针对这种网格... 详细信息

提出了一种三维重构算法,采用分形插值方法对三维物体多个序列切片的特征数据点进行插值重构。传统的三维迭代函数系统(IFS)分形插值算法只适用于矩形剖分,而序列切片的特征数据形成的网格往往是不规则的四边形网格。该文针对这种网格的特点和分形插值的自相似性,设计了该算法。实验结果证明了该算法的实用性和有效性。

关键词：分形插值三维重构迭代函数系统

Expression of matrix metalloproteinases in recurred stage IB non small cell lung cancer

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Expression of matrix metalloproteinases in recurred stage IB...

第13届全国肺癌学术大会

作者： Songnan Zhang Hai Cui wenbiao jin Xian Cui Yongmin jin YuZhen Cui XiongHu Shen Department of Oncology Affiliated Hospital of Yanbian University

＜正＞We aimed to clarify the prime role of matrix metalloproteinases（MMPs） in recurrence of stage IB non-small cell lung cancer（NSCLC）.Ten stage IB NSCLC patients presenting recurrence were selected and compared with the other ten stage IB NSCLC patients without recurrence since biopsied from August,2009 to December,2011. Semiquantitative RT-PCR and real-time PCR were performed subsequently to evaluate

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岩石节理二值图像的细化

微计算机信息 2007年第2X期23卷 294-296页

作者：李锐金文标重庆邮电大学重庆400065

分析岩石节理图片,细化是关键的一步,它是分析节理拓扑结构的基础。由于二值节理图片噪声大,节理内部有空洞等特点,直接用现有细化算法得到的结果中存在大量毛刺和并非真实骨架的环形结构。本文针对这些问题提出了一种算法,把彩色图像... 详细信息

分析岩石节理图片,细化是关键的一步,它是分析节理拓扑结构的基础。由于二值节理图片噪声大,节理内部有空洞等特点,直接用现有细化算法得到的结果中存在大量毛刺和并非真实骨架的环形结构。本文针对这些问题提出了一种算法,把彩色图像中的信息加到了二值图像处理过程中。本算法细化后的结果更好地反映了岩石节理的骨架。

关键词：岩石节理细化图像处理