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An Efficient and Reliable Assay for Investigating the Effects of Hypoxia/Anoxia on Drosophila

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Neuroscience Bulletin 2018年第2期34卷 397-402页

作者： Yiling Xia wangchao Xu Shiquan Meng Nastasia K.H.Lim wenan wang Fu-De Huang Department of Neurology Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine Department of Neurology Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiaotong University School of Medicine Institute of Neuroscience University of the Chinese Academy of Sciences Chinese Academy of Sciences Shanghai Advanced Research Institute University of the Chinese Academy of Sciences Chinese Academy of Sciences

Stroke is a leading cause of death worldwide. Up to one thousand potential drugs or interventions have been developed to treat stroke, out of which;60 have gone on to clinical trials. However, none of them has been successful. New insights into the molecular and cellular mechanisms of ischemia-induced injury are needed for discovering new therapeutic targets. Recently, Drosophila has been used to uncover new hypoxia-related genes. In this study, we describe an efficient and reliable assay with a sophisticated apparatus for studying the effects of oxygen deprivation on flies. Using this assay, wild-type flies were exposed to an anoxic environment for varying lengths of time, then the cumulative death rate and mobility recovery were systematically analyzed. We found that anoxia for over one hour caused lethality. The cumulative death rate on day 5 after anoxia was linearly and positively correlatedwith the duration of anoxia, and reached 50% when the duration was 2.5 h–3 h. We also found that the mobility recovery in normoxia was slow, as the climbing ability remained largely unchanged 4 h–6 h after 2.5-h of anoxia.We suggest that 2.5 h–3 h of anoxia and 4 h–6 h of recovery before mobility analysis are appropriate for future use of the anoxia assay.

关键词： Hypoxia Anoxia Drosophila Mortality Locomotor activity

Pgant4 and Tango1 Mediate Anoxia and Reoxygenation Injury

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Neuroscience Bulletin 2020年第12期36卷 1552-1557页

作者： Qingqing Du Nastasia KHLim Yiling Xia wangchao Xu Qichao Zhang Liyao Zhang Fude Huang wenan wang Department of Neurology Xinhua Hospital Affiliated to Shanghai Jiaotong University School of MedicineShanghai 202150China Department of Neurology Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiaotong University School of MedicineShanghai 202150China Shanghai Institute of Materia Medica University of the Chinese Academy of SciencesShanghai 201203China Shanghai Advanced Research Institute University of the Chinese Academy of SciencesShanghai 201210China Nuo-beta Pharmaceutical Technology(Shanghai)Co.Ltd Shanghai 201210China Institute of Neuroscience University of the Chinese Academy of SciencesShanghai 200031China Sino-Danish College University of the Chinese Academy of SciencesBeijing 100049China

Dear Editor,Diseases caused by ischemia,including coronary artery disease and stroke,are a leading cause of death worldwide[1].Restoration of the blood and oxygen supply after restriction also causes tissue damage by activating a number of pathological pathways,such as inflammatory,oxidative stress,and cell death pathways that are mediated by microRNAs and hypoxia-inducible factors[1,2].

关键词： death damage ischemia

Gastric cancer prognostic marker DDX54 plays an important role in gastric cancer immunity

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Gastric cancer prognostic marker DDX54 plays an important ro...

浸没燃烧式气化器SCV的RAM分析及可用度优化

2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青...

作者： wenan wang Yali Zhang An Zhang Wenjie wang Changan Guo Hongbin Liu Department of Hematology Lanzhou University First Hospital epartment of General Surgery Lanzhou University Second Hospital Department of Emergency Medicine Lanzhou University Second Hospital Department of general surgery 940 Hospital of the joint logistics support force of the Chinese people's Liberation Army

Background: The high expression of DEAD-Box Helicase 54 promotes the occurrence of colorectal cancer,however, the relationship between its role in gastric cancer and immune cell infiltration remain unclear.Methods: Use ENCORI and GEPIA databases to determine the relationship between DDX54 and CASC19.Use Oncomine, TIMER, The Human Protein Atlas to find the difference between DDX54 in normal tissues and cancer tissues, use Kaplan-Meier Plotter, UALCAN, PrognoScan database for prognostic analysis, and finally use TIMER Database for immune infiltration analysis.Results: Gene and protein levels proved that DDX54 is expressed differently in a variety of cancers,especially in gastric cancer and colorectal cancer, and the prognosis is poor in gastric cancer（OS,HR=1.61, P=1.4 E-05;PFS, HR=1.41, P=0.0077）. DDX54 is positively correlated with CD4+ T cells,and negatively correlated with the other 5 immune cells, regardless of STAD or COAD. Immune marker analysis DDX54 is associated with a variety of immune cells. There is a strong positive correlation between gastric cancer and Regulatory T cell.Conclusion: DDX54 is highly expressed in gastric cancer tissues, and the higher the expression, the worse the prognosis, which may be related to the suppression of the anti-tumor effect of normal immune cells and the recruitment of Treg infiltration. This indicates that it is a valuable prognostic and tumor marker for gastric cancer.

关键词： Gastric cancer DEAD-Box Helicase 54（DDX54） Prognosis Bioinformatics analysis Immunology

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高校化学工程学报 2024年第2期38卷 302-309页

作者：周文安王海清陈桂海韩思杰中国石油大学(华东)安全科学与工程系山东青岛266580 安全与运营管理部深圳市燃气集团股份有限公司广东深圳518040

浸没燃烧式气化器(SCV)是液化天然气LNG应急调峰站的关键核心系统,系统中出现设备故障会导致天然气供应中断,将造成显著社会影响与生产损失。为了评估SCV系统的生产性能及提高运行稳定性,提出并建立SCV-RAM分析模型,应用蒙特卡洛模拟仿... 详细信息

浸没燃烧式气化器(SCV)是液化天然气LNG应急调峰站的关键核心系统,系统中出现设备故障会导致天然气供应中断,将造成显著社会影响与生产损失。为了评估SCV系统的生产性能及提高运行稳定性,提出并建立SCV-RAM分析模型,应用蒙特卡洛模拟仿真定量辨识SCV的瓶颈单元设备并针对性设计改进方案。结果表明,SCV系统易发生故障的单元按贡献度依次分别是:助燃空气单元、循环冷却水单元,其中导致系统不可用度增大的具体关键设备为水泵、电机以及鼓风机。据此对关键设备的预防性维修间隔进行优化,SCV系统的全寿命周期统计可用度从90.17%提高到94.93%,总停机时间减少了3478h。分析结果有助于优化SCV系统的维修资源分配,为实现SCV系统长周期稳定运行提供技术支持。

关键词：浸没燃烧式气化器(SCV) 可靠性、可用性和维护性分析(RAM分析) 关键设备可用度优化

Assessment of tree-ring mercury radial translocation and age effect in Masson pine:Implications for historical atmospheric mercury reconstruction

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Journal of Environmental Sciences 2024年第4期138卷 266-276页

作者： Xu Liu Xun wang Dingyong wang College of Resources and Environment Southwest UniversityChongqing 400715China State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China

The tree ring has been regarded as an emerging archive to reconstruct historical atmospheric mercury(Hg)trends,but with the large knowledge gaps in the reliability.In this study,we comprehensively evaluated the Hg source,radial translocation and age effect of Masson pine(Pinus massoniana)tree ring at Mt.Jinyun in Chongqing,to assess the suitability of such tree ring as the archive of atmospheric Hg.Results showed that distinct variabilities among Masson pine tree-ring Hg concentration profiles.The Hg concentration significantly increased along with stem height(P<0.05),indicating the Hg in tree rings mainly derived from foliage uptake atmospheric Hg.We found a distinct age effect that the tree ring of young trees had the higher Hg concentration.Besides,we used the advection-diffusion model to demonstrate how Hg concentration shifted by the advection or/and diffusion in tree rings.The modeling results showed that the advection induced radial translocation during the young growth period of tree was a plausible mechanism to result in the tree-ring Hg record largely different from the trend of anthropogenic Hg emissions in Chongqing.We finally suggest that in further Hg dendrochemistry,better discarding the tree-ring Hg profile of the young growth period to reduce impacts of the radial translocation and age effect.

关键词： Masson pine Tree-ring mercury Radial translocation Tree age effect

激光加工表面织构对配流盘重载摩擦副摩擦磨损特性的影响

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热加工工艺 2022年第22期51卷 97-101页

作者：王文安刘志奇谢志鸣太原科技大学机械工程学院山西太原030000

柱塞泵配流盘-缸体摩擦副在柱塞泵的运行之中导致的运行磨损及磨损产生的异物二次磨损是影响配流盘-缸体使用寿命的重要因素。采用激光加工技术在配流盘常用基础材料59黄铜表面加工微织构。在中科凯华CFT-1摩擦磨损机上,选用常见缸体基... 详细信息

柱塞泵配流盘-缸体摩擦副在柱塞泵的运行之中导致的运行磨损及磨损产生的异物二次磨损是影响配流盘-缸体使用寿命的重要因素。采用激光加工技术在配流盘常用基础材料59黄铜表面加工微织构。在中科凯华CFT-1摩擦磨损机上,选用常见缸体基础材料45钢以面-面接触形式分别对有织构和无织构的配流盘-缸体摩擦副试样在干磨擦与混合摩擦(润滑)条件下的摩擦磨损性能进行了试验。分析了不同织构参数在不同摩擦条件下对试样摩擦磨损的影响。结果表明:织构必须在混合摩擦条件下才能产生减摩效应;与未加工织构的铜合金试样表面相比,合理的织构参数可使织构在液压工况条件下稳定留存,并发挥其减摩作用,使磨损量显著降低。

关键词：激光加工成形摩擦副微织构摩擦磨损性能质量磨损率

Pushing the boundaries: an interview with Dae-Hyun Kim on terahertz devices

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National Science Review 2024年第3期11卷 42-45页

作者： Defu wang the Center for Carbon-based Electronics School of Electronics Peking University

Ⅲ–Ⅴ compound semiconductors, such as InGaAs/InAlAs, exhibit exceptional carrier transport properties, establishing them as fundamental elements in terahertz(THz) applications crucial for the development of 6G netwo...

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Enrichment of anammox biomass during mainstream wastewater treatment driven by achievement of partial denitrification through the addition of bio-carriers

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Journal of Environmental Sciences 2024年第3期137卷 181-194页

作者： Yuqing Ma Bo wang Xiaodi Li Shuo wang Wen wang Yongzhen Peng National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology Engineering Research Center of BeijingBeijing University of TechnologyBeijing 100124China

Anammox is widely considered as the most cost-effective and sustainable process for nitrogen removal.However,how to achieve the enrichment of anammox biomass remains a challenge for its large-scale application,especially in mainstream wastewater treatment.In this study,the feasibility of enrichment of anammox biomass was explored through the realization of partial denitrification and the addition of bio-carriers.By using ordinary activated sludge,a sequencing batch reactor(SBR)followed by an up-fow anaerobic sludge bed(UASB)was operated at 25±2℃ for 214 days.The long-term operation was divided into five phases,in which SBR and UASB were started-up in Phases I and II,respectively.By eliminating oxygen and adjusting the infow ratios in Phases III-V,advanced nitrogen removal was achieved with the effuent total nitrogen being 4.7 mg/L and the nitrogen removal efficiency being 90.5%in Phase V.Both in-situ and ex-situ activity tests demonstrated the occurrence of partial denitrification and anammox.Moreover,16S rRNA high-throughput sequencing analysis revealed that Candidatus Brocadia was enriched from below the detection limit to in biofilms(0.4%in SBR,2.2%in UASB)and the foc sludge(0.2%in SBR,1.3%in UASB),while Thauera was mainly detected in the foc sludge(8.1%in SBR,8.8%in UASB),which might play a key role in partial denitrification.Overall,this study provides a novel strategy to enrich anammox biomass driven by rapid achievement of partial denitrification through the addition of bio-carriers,which will improve large-scale application of anammox processes in mainstream wastewater treatment.

关键词： Enrichment Anammox biomass Bio-carriers Partial denitrification Mainstream wastewater

Performance and mechanism of La-Fe metal-organic framework as a highly efficient adsorbent for fluoride removal from mine water

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Journal of Environmental Sciences 2024年第5期139卷 245-257页

作者： Chaomin Jia Jianbing wang Huijiao wang Sichao Zhu Xiaohui Zhang Yuxiang wang School of Chemical&Environmental Engineering China University of Mining&Technology-BeijingBeijing 100083China Beijing Polytechnic Beijing 100176China Chinese Society for Urban Studies Beijing 100835China

Water fluoride pollution has caused non-negligible harm to the environment and humans,and thus it is crucial to find a suitable treatment technology.In this study,La-Fe@PTA adsorbent was synthesized for the defluoridation of mine water.The results showed that the optimum conditions for defluoridation by La-Fe@PTA were p H close to 7.0,the initial F-concentration of 10 mg/L,the dosage of 0.5 g/L and the adsorption time of 240 min.Compared with SO_4^(2-),Cl^(-),NO_(3)^(-),Ca^(2+)and Mg^(2+),CO_(3)^(2-)and HCO_(3)^(-)presented severer inhibition on fluoride uptake by La-Fe@PTA.The adsorption process fits well with the pseudo-second-order kinetic model and Freundlich model,and the maximum adsorption capacity of Langmuir model was 95 mg/g.Fixed-bed adsorption results indicated that fluoride in practical fluorinated mine water could be effectively removed from 3.6 mg/L to less than 1.5 mg/L within130 bed volume(BV)by using 1.5 g La-Fe@PTA.Furthermore,the adsorbent still had good adsorption capacity after regeneration,which confirms the great application potential of La-Fe@PTA as a fluoride ion adsorbent.The mechanism analysis showed that La-Fe@PTA adsorption of fluorine ions is a physicochemical reaction driven by electrostatic attraction and ion exchange.

关键词： Adsorption La-Fe@PTA Fluoride ion Mine water Mechanism