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Survey of antioxidant capacity and phenolic composition of blueberry,blackberry,and strawberry in Nanjing

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第2期13卷 94-102页

作者： Wu-yang HUANG Hong-cheng ZHANG wen-xu liu Chun-yang LI Department of Functional Food and Bioactive Compounds Institute of Farm Product ProcessingJiangsu Academy of Agricultural SciencesNanjing 210014China Bee Research Institute Chinese Academy of Agricultural SciencesBeijing 100093China College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China

Berries are a good source of natural antioxidants. In the present study, the total antioxidant capacity and phenolic composition of three berry fruits （blueberry, blackberry, and strawberry） cultivated in Nanjing were investigated. Blueberry, with a Trolox equivalent antioxidant capacity （TEAC） value of 14.98 mmol Trolox/100 g dry weight （DW） exhibited the strongest total antioxidant capacity using both the 2,2-azinobis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt （ABTS） and the 2,2-diphenyl-l-picrylhydrazyl （DPPH） methods. Blueberry also had the highest total phenolic content （TPC, 9.44 mg gallic acid/g DW）, total flavonoid content （TFC, 36.06 mg rutin/g DW）, and total anthocyanidin content （TAC, 24.38 mg catechin/g DW）. A preliminary analysis using high performance liquid chromatography （HPLC） showed that the blueberry, blackberry, and strawberry samples tested contained a range of phenolic acids （including gallic acid, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid, ferulic acid, eUagic acid, and cinnamic acid） and various types of flavonoids （flavone： luteolin; flavonols： rutin, myricetin, quercetrin, and quercetin; flavanols： gallocatechin, epigallocatechin, catechin, and catechin gallate; anthocyanidins： malvidin-3-galactoside, malvidin-3-glucoside, and cyanidin）. In particular, the blueberries had high levels of proanthocyanidins and anthocyanidins, which might be responsible for their strong antioxidant activities. These results indicate a potential market role for berries （especially blueberries） as a functional food ingredient or nutraceutical.

关键词： Berries Antioxidants Phenolics Flavonoids Anthocyanidins

Template synthesis of copper azide primary explosive through Cu2O@HKUST-1 core-shell composite prepared by “bottle around ship” method

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Defence Technology（防务技术） 2023年第7期25卷 99-111页

作者： xu-wen liu Yan Hu Jia-heng Hu Jia-xin Su Cai-min Yang Ying-hua Ye Rui-qi Shen School of Chemistry and Chemical Engineering Nanjing University of Science and TechnologyNanjing210094China Micro-Nano Energetic Devices Key Laboratory Ministry of Industry and Information TechnologyNanjing210094China

Copper azide(CA), as a primary explosive with high energy density, has not been practically used so far because of its high electrostatic sensitivity. The Cu2O@HKUST-1 core-shell structure hybrid material was synthesized by the “bottle around ship” methodology in this research by regulating the dissolution rate of Cu2O and the generation rate of metal-organic framework(MOF) materials. Cu2O@HKUST-1 was carbonized to form a Cu O@porous carbon(CuO@PC) composite material. CuO@PC was synthesized into a copper azide(CA) @PC composite energetic material through a gas-solid phase in-situ azidation *** is encapsulated in PC framework, which acts as a nanoscale Faraday cage, and its excellent electrical conductivity prevents electrostatic charges from accumulating on the energetic material’s surface. The CA@PC composite energetic material has a CA content of 89.6%, and its electrostatic safety is nearly 30times that of pure CA(1.47 mJ compared to 0.05 mJ). CA@PC delivers an outstanding balance of safety and energy density compared to similar materials.

关键词： Composite energetic materials Copper azide Carbonization Template method Core-shell composite Electrostatic safety

Electrochemical treatment of COD in biologically pretreated coking wastewater using Ti/RuO2-IrO2 electrodes combined with modified coke

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Journal of Coal Science & Engineering(China) 2011年第4期17卷 426-430页

作者： HE xu-wen liu Li-yuan GONG Jing-wen WANG Jian-bing QIN Qiang WANG Hao School of Chemical and Environmental Engineering China University of Mining and Technology （Beijing） Beijing 100083China

The electrochemical treatment of COD contained in biologically pretreated coking wastewater treated by a three-dimensional electrode system with modified coke as the particle electrode was investigated. And the electrochemical perromance of the coke modified with various active components was studied. The results show that the coke modified with Fe（NO3）2 has the lowest energy consumption and higher COD removal rate under the same condition, and the modified coke has better surface characteristics for the purpose of this study. In addition, the kinetic constant was also calculated. The study shows that the three-dimensional electrode system with Fe （NO3）z-modified coke can give a satisfactory solution in biologically pretreated coking wastewater.

关键词： three-dimensional electrode Ti/RuO2-IrO2 anode Fe (NO3)2-modified coke coking wastewater

Constituents from Aerial Parts of Bidens ceruna L. and Their DPPH Radical Scavenging Activity

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Chemical Research in Chinese Universities 2009年第3期25卷 328-331页

作者： ZHU Na LI xu-wen liu Gui-ying SHI Xiao-lei GUI Ming-yu SUN Chang-qing JIN Yong-ri College of Chemistry Jilin University Changchun 130012 P. R. China

A new chalcone glycoside, butein-4-methoxyl-4′-O-（6＂-O-acetyl）-β-D-glucopyranoside（2）, along with seven known compounds, namely, quercitrin（1）, luteolin-7-O-β-D-glucopyranoside（3）, butein-4＇-O-β-D-glucopyranoside（4）, butein-4-methoxyl-4＇-O-β-D-glucopyranoside（5）, butin-7-O-β-D-glucopyranoside（6）, isoquercitrin（7）, and sulfurein（8） was isolated from aerial parts of Bidens ceruna L. Their structures were elucidated on the basis of spectroscopic studies. Compounds 1-8 were tested for their DPPH radical scavenging activity and, except compounds 2 and 5, other compounds showed scavenging activitv.

关键词： Bidens ceruna L. Constituent Flavonoid DPPH radical Scavenging activity

Recombinant tissue plasminogen activator plus heparin compared with heparin alone for patients with acute submassive pulmonary embolism: one-year outcome

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Journal of Geriatric Cardiology 2013年第4期10卷 323-329页

作者： Yu-Hong Mi Ying Liang Yan-Hui Lu Ya-Min Li wen-xu liu Wang Qian Emergency Intensive Care Unit in Respiration Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Department of Ultrasound Cardiography Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Department of Nuclear Medicine Beijing Anzhen Hospital Capital Medical University Beijing 100029 China

OBJECTIVE: To evaluate the long-term effects of thrombolysis on patients with submassive pulmonary embolism (PE)。 METHODS: Data of 136 patients with acute submassive PE and low risk of bleeding were prospectively collected from January 2005 to October 2011 in a single medical center。 Patients received recombinant tissue plasminogen activator (r-tPA) plus low molecular weight heparin (LMWH, TT group, n = 79) or LMWH alone (AT group, n = 57), depending on treating physician's recommendation and patient's preference。 Echocardiography was performed at admission, 24 h, 6 and 12 months to evaluate right ventricular function。 Computed tomography pulmonary angiography (CTPA) and lung perfusion scan were performed on admission, at 7 days, 6 and 12 months to evaluate clot burden。 RESULTS: Seventy-nine patients received r-tPA plus LMWH (TT group) while 57 received LMWH alone (AT group)。 The baseline characteristics and risk factors did not differ between the two groups。 Respiratory rate, heart rate, and systolic blood pressure improved within two hours in both groups。 Systolic pulmonary arterial pressure and tricuspid regurgitation improved to a greater extent in the TT group at 24 h, and at 12 months (P < 0。001), as compared to those in the AT group。 At one week, and 12 months, clot burden decreased more in AT group, as compared to that in AT group (P < 0.001)。 There was no death due to bleeding in both groups。 Recurrent PE were similar in both groups (2.5% in TT vs。 1.8% in AT)。 The rates of minor hemorrhages were 6.3% in TT group and 1.8% in AT group (P < 0.05)。 In submassive PE patient who has low risk of bleeding, thrombolysis plus anticoagulation can lead to greater improvement of right ventricular dysfunction and clot burden reduction as compared to anticoagulation therapy alone。

关键词： Pulmonary embolism Right ventricle dysfunction Thrombolysis Anticoagulation Bleeding

Expression of a Carrot 36 kD Antifreeze Protein Gene Improves ColdStress Tolerance in Transgenic Tobacco

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Forestry Studies in China 2005年第4期7卷 11-15页

作者： xu wen-li liu Mei-qin Shen Xin Lu Cun-fu College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China

Antifreeze proteins （AFPs） enable organisms to survive under cold conditions, and have great potential in improving cold tolerance of cold-sensitive plants, In order to determine whether expression of the carrot 36 kD antifreeze protein gene confers improved cold-resistant properties to plant tissues, we tried to obtain transgenic tobacco plants which expressed the antifreeze protein. Cold, salt, and drought induced promoter Prd29A was cloned using PCR from Arabidopsis. Two plant expression vectors based on pBI121 were constructed with CaMV35S：AFP and Prd29A：AFP. Tobacco plantlets were transformed by Agrobacterium-medicated transformation. PCR and Southern blotting demonstrated that the carrot 36 kD afp gene was successfully integrated into the genomes of transformed plantlets. The expression of the afp gene in transgenic plants led to improved tolerance to cold stress. However, the use of the strong constitutive 35S cauliflower mosaic virus （CaMV） promoter to drive expression of afp also resulted in growth retardation under normal growing conditions. In contrast, the expression of afp driven by the stress-inducible Prd29A promoter from Arabidopsis gave rise to minimal effects on plant growth while providing an increased tolerance to cold stress condition （2℃）. The results demonstrated the prospect of using Prd29A-AFP transgenic plants in cold-stressed conditions that will in turn benefit agriculture.

关键词： antifreeze protein gene stress inducible-promoter tobacco genetic transformation cold tolerance

Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

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Neural Regeneration Research 2025年第4期20卷 1124-1134页

作者： Yanting liu Wei Huang Jiayi wen Xin Xiong Ting xu Qi Wang Xiusheng Chen Xianxian Zhao Shihua Li Xiaojiang Li Weili Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan UniversityJinan UniversityGuangzhouGuangdong ProvinceChina

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal ***,it remains largely unclear how PINK1 and Parkin are expressed in mammalian *** has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small *** this issue is critical for elucidating the in vivo roles of PINK1 and ***,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate *** the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate *** found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey ***1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable *** double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial *** findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

关键词： neurodegeneration Parkin Parkinson’s disease PINK1 subcellular distribution

Synthesis of n-Butyl Acetate by Microwave Irradiation Under the Assistance of Solid-Liquid Phase Transfer Catalysis Without Solvent

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Chemical Research in Chinese Universities 1992年第3期8卷 324-326页

作者： xu wen-guo, liu Fu-an, YU Ai-min, LI Yao-xian, JIN Shen-xi,liu Jun and JIN Qin-han (Department of Chemistry, Jilin University, Changchun, 130023)

Introduction Microwave irradiation has been very widely used in heating, cooking and brewing. Several papers which describe the use of domestic microwave ovens to perform rapid organic synthesis in solution have been published. The high heating efficiency gives rise to remarkable rate of reaction and dramatic reduction of reaction time. Nevertheless, its application seems to be limited to these procedures because of

关键词： Microwave irradiation Phase transfer catalysis Synthesis n-Butyl ace-tate Inorganic support

PI3K/AKT signaling and neuroprotection in ischemic stroke:molecular mechanisms and therapeutic perspectives

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Neural Regeneration Research 2025年第10期20卷 2758-2775页

作者： Tianlong liu Xiaolin Li Xiaowei Zhou Wei Chen Aidong wen Minna liu Yi Ding Department of Pharmacy Xijing HospitalFourth Military Medical UniversityXi’anShaanxi ProvinceChina Department of Pharmacy The 940 Hospital of Joint Logistics Support Forces of Chinese PLALanzhouGansu ProvinceChina Department of Pharmacy The 944 Hospital of Joint Logistics Support Forces of Chinese PLAJiuquanGansu ProvinceChina Department of Nephrology The 940 Hospital of Joint Logistics Support Forces of Chinese PLALanzhouGansu ProvinceChina

It has been reported that the PI3K/AKT signaling pathway plays a key role in the pathogenesis of ischemic *** a result,the development of drugs targeting the PI3K/AKT signaling pathway has attracted increasing attention from *** article reviews the pathological mechanisms and advancements in research related to the signaling pathways in ischemic stroke,with a focus on the PI3K/AKT signaling *** key findings include the following:(1)The complex pathological mechanisms of ischemic stroke can be categorized into five major types:excitatory amino acid toxicity,Ca^(2+)overload,inflammatory response,oxidative stress,and apoptosis.(2)The PI3K/AKT-mediated signaling pathway is closely associated with the occurrence and progression of ischemic stroke,which primarily involves the NF-κB,NRF2,BCL-2,mTOR,and endothelial NOS signaling pathways.(3)Natural products,including flavonoids,quinones,alkaloids,phenylpropanoids,phenols,terpenoids,and iridoids,show great potential as candidate substances for the development of innovative anti-stroke medications.(4)Recently,novel therapeutic techniques,such as electroacupuncture and mesenchymal stem cell therapy,have demonstrated the potential to improve stroke outcomes by activating the PI3K/AKT signaling pathway,providing new possibilities for the treatment and rehabilitation of patients with ischemic *** investigations should focus on the direct regulatory mechanisms of drugs targeting the PI3K/AKT signaling pathway and their clinical translation to develop innovative treatment strategies for ischemic stroke.

关键词： apoptosis autophagy inflammation ischemic stroke neuroprotection oxidative stress pathogenesis phosphatidylinositol 3-kinase protein kinase B therapeutics