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Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alphasynuclein

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Neural Regeneration Research 2012年第2期7卷 85-90页

作者： Tao Chen Xiaoping Liao guoqiang wen Yidong Deng min guo Zhigang Long Feng Ouyang Department of Neurology Hainan Provincial People＇s Hospital Haikou 570311 Hainan Province China

The specific and effective a-synuclein RNA interference （RNAi） plasmids, and the a-synuclein-pEGFP recombinant plasmids were co-transfected into human embryonic kidney 293 （HEK293） cells using the lipofectamine method. Using an inverted fluorescence microscope, a-synuclein proteins were observed to aggregate in the cytoplasm and nucleus. Wild-type a-synuclein proteins co-localized with mitochondria. Hematoxylin-eosin staining revealed round eosinophilic bodies （Lewy body-like inclusions） in the cytoplasm of some cells transfected with a-synuclein-pEGFP plasmid. However, the formation of Lewy body-like inclusions was not observed following transfection with the RNAi pSYN-1 plasmid. RNAi blocked Lewy body-like inclusions in the cytoplasm of HEK293 cells induced by wild-type a-synuclein overexpression, but RNAi did not affect the subcellular localization of wild-type a-synuclein in mitochondria.

关键词： RNA interference a-synuclein gene subcellular localization inclusion Parkinson'sdisease neural regeneration

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云南地区地壳磁异常与地质构造

地球物理学报 2017年第9期60卷 3493-3504页

作者：文丽敏康国发白春华高国明郑安燃安柏林云南大学地球物理系昆明650091

根据美国地球物理数据中心的地磁场模型(NGDC-720),研究云南地区地壳磁异常的空间变化,包括磁异常及其梯度的分布、磁异常随高度的衰减、不同波长成分对总体磁异常的贡献.比较磁异常与重力异常、大地热流、地震活动等地球物理信息的异... 详细信息

根据美国地球物理数据中心的地磁场模型(NGDC-720),研究云南地区地壳磁异常的空间变化,包括磁异常及其梯度的分布、磁异常随高度的衰减、不同波长成分对总体磁异常的贡献.比较磁异常与重力异常、大地热流、地震活动等地球物理信息的异同特征,探讨地壳磁异常与地质构造的关系.研究结果表明:丽江—小金河、红河断裂带以西的三江、滇西造山带的异常较弱,菱形地块的异常相对较强.研究区域的地壳磁异常主要是弱磁性基底背景下叠加的浅源磁性体产生.卫星磁异常显示滇中坳陷区具有清晰的偶极场特征.沿红河断裂带分布的正负磁异常带与断裂构造走向一致.围限菱形地块的丽江—小金河断裂、红河断裂、康定—奕良—水城断裂和弥勒—师宗—水城断裂带是磁异常的强弱过渡带.强烈地震发生的地点、大地热流值高的地区,地壳磁异常为负值或相对较弱.

关键词：地磁场模型地壳磁异常地质构造云南地区

Effect of Al addition on microstructure and mechanical properties of Mg-Gd-Zn alloys

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Transactions of Nonferrous Metals Society of China 2022年第3期32卷 824-837页

作者： Zhi-bing DING Shuai ZHANG Yu-hong ZHAO Dong-rui CHEN Li-hua ZOU Zhi-gang CHEN wen-min guo Zai-jun SU Hua HOU College of Mechanical and Energy Engineering Shaoyang UniversityShaoyang 422000China Key Laboratory of Hunan Province for Efficient Power System and Intelligent Manufacturing Shaoyang UniversityShaoyang 422000China College of Materials Science and Engineering North University of ChinaTaiyuan 030051China

The microstructure evolution and mechanical properties of Mg−15.3Gd−1Zn alloys with different Al contents(0,0.4,0.7 and 1.0 wt.%)were *** analysis indicates that the addition of 0.4 wt.%Al facilitates the formation of 18R-LPSO phase(Mg12Gd(Al,Zn))in the Mg−Gd−Zn *** contents of Al11Gd3 and Al2Gd increase with the increase of Al content,while the content of(Mg,Zn)_(3)Gd *** homogenization treatment,(Mg,Zn)_(3)Gd,18R-LPSO and some Al11Gd3 phases are transformed into the high-temperature stable 14H-LPSO *** particulate Al−Gd phases can stimulate the nucleation of dynamic recrystallization by the particle simulated nucleation(PSN)*** tensile strength of the as-rolled alloys is improved remarkably due to the grain refinement and the fiber-like reinforcement of LPSO *** precipitation of theβ′phase in the peak-aged alloys can significantly improve the *** peak-aged alloy containing 0.4 wt.%Al achieves excellent mechanical properties and the UTS,YS and elongation are 458 MPa,375 MPa and 6.2%,respectively.

关键词： Mg-Gd-Zn-Al alloy long-period stacking ordered(LPSO)phase β′phase mechanical properties

The detection, extraction and parameter estimation of extreme-mass-ratio inspirals with deep learning

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 86-96页

作者： Qianyun Yun wen-Biao Han Yi-Yang guo He Wang minghui Du Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Shanghai Astronomical Observatory Chinese Academy of Sciences School of Physics and Astronomy Shanghai Jiao Tong University School of Astronomy and Space Science University of Chinese Academy of Sciences Taiji Laboratory for Gravitational Wave Universe (Beijing/Hangzhou) University of Chinese Academy of Sciences Lanzhou Center for Theoretical Physics Key Laboratory of Theoretical Physics of Gansu Province Key Laboratory of Quantum Theory and Applications of MoE Lanzhou University Institute of Theoretical Physics & Research Center of Gravitation Lanzhou University International Centre for Theoretical Physics Asia-Pacific (ICTP-AP) University of Chinese Academy of Sciences (UCAS) Center for Gravitational Wave Experiment National Microgravity Laboratory Institute of Mechanics Chinese Academy of Sciences

One of the primary goals of space-borne gravitational wave detectors is to detect and analyze extreme-mass-ratio inspirals(EMRIs). This task is particularly challenging because EMRI signals are complex, lengthy, and faint. In this work, we introduce a2-layer convolutional neural network(CNN) approach to detect EMRI signals for space-borne detectors, achieving a true positive rate(TPR) of 96.9% at a 1% false positive rate(FPR) for signal-to-noise ratio(SNR) from 50 to 100. Especially, the key intrinsic parameters of EMRIs such as the mass, spin of the supermassive black hole(SMBH) and the initial eccentricity of the orbit can also be inferred directly by employing a neural network. The mass and spin of the SMBH can be determined at 99% and 92% respectively. This will greatly reduce the parameter spaces and computing cost for the following Bayesian parameter *** model also has a low dependency on the accuracy of the waveform model. This study underscores the potential of deep learning methods in EMRI data analysis, enabling the rapid detection of EMRI signals and efficient parameter estimation.

关键词： gravitational wave EMRIs deep-learing

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单峰分布枢轴量的等尾与等高置信区间的比较

数学的实践与认识 2016年第24期46卷 156-160页

作者：康文倩郭民之李盛云南师范大学数学学院云南昆明650500

对枢轴量G的分布具有单峰密度函数的情形,证明了G的最短置信区间是满足置信区间端点密度函数值"等高"条件的置信区间.还对枢轴量分布为正态分布、t分布、卡方分布、F分布、伽玛分布和对数正态分布情形下,在Excel中进行了搜索式数值计算... 详细信息

对枢轴量G的分布具有单峰密度函数的情形,证明了G的最短置信区间是满足置信区间端点密度函数值"等高"条件的置信区间.还对枢轴量分布为正态分布、t分布、卡方分布、F分布、伽玛分布和对数正态分布情形下,在Excel中进行了搜索式数值计算,并列表比较了"等尾"和"等高"情形下置信区间的长度,验证了上述分析结论.另外,还讨论了枢轴量的最短置信区间与枢轴量中所含参数的最短置信区间的关系.

关键词：单峰分布枢轴量置信区间最短条件列表比较

Porous Organic Cage‑Based Quasi‑Solid‑State Electrolyte with Cavity‑Induced Anion‑Trapping Effect for Long‑Life Lithium Metal Batteries

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Nano-Micro Letters 2025年第2期17卷 376-386页

作者： Wei-min Qin Zhongliang Li wen‑Xia Su Jia‑min Hu Hanqin Zou Zhixuan Wu Zhiqin Ruan Yue‑Peng Cai Kang Li Qifeng Zheng School of Chemistry South China Normal UniversityGuangzhou 510006People’s Republic of China Key Laboratory of Functional Metal‑Organic Compounds of Hunan Province College of Chemistry and Material ScienceHengyang Normal UniversityHengyang 421008People’s Republic of China Guangzhou Key Laboratory of Energy Conversion and Energy Storage Materials Guangzhou 510006People’s Republic of China

Porous organic cages(POCs)with permanent porosity and excellent host–guest property hold great potentials in regulating ion transport behavior,yet their feasibility as solid-state electrolytes has never been testified in a practical ***,we design and fabricate a quasi-solid-state electrolyte(QSSE)based on a POC to enable the stable operation of Li-metal batteries(LMBs).Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC,the resulting POC-based QSSE exhibits a high Li+transference number of 0.67 and a high ionic conductivity of 1.25×10^(−4) S cm^(−1) with a low activation energy of 0.17 *** allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 *** a proof of concept,the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85%capacity retention after 1000 ***,our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems,such as Na and K batteries.

关键词： Porous organic cage Cavity-induced anion-trapping Quasi-solid-state electrolyte Homogeneous Li+flux Lithium metal battery

Cortico-striatal gamma oscillations are modulated by dopamine D3 receptors in dyskinetic rats

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Neural Regeneration Research 2025年第4期20卷 1164-1177页

作者： Pengfei Wang Yuewei Bi min Li Jiazhi Chen Zhuyong Wang Huantao wen ming Zhou minjie Luo Wangming Zhang Neurosurgery Center Department of Pediatric NeurosurgeryGuangdong Provincial Key Laboratory on Brain Function Repair and RegenerationZhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

Long-term levodopa administration can lead to the development of levodopa-induced *** oscillations are a widely recognized hallmark of abnormal neural electrical activity in levodopa-induced ***,studies have reported increased oscillation power in cases of levodopa-induced ***,little is known about how the other electrophysiological parameters of gamma oscillations are altered in levodopa-induced ***,the role of the dopamine D3 receptor,which is implicated in levodopa-induced dyskinesia,in movement disorder-related changes in neural oscillations is *** found that the cortico-striatal functional connectivity of beta oscillations was enhanced in a model of Parkinson’s ***,levodopa application enhanced cortical gamma oscillations in cortico-striatal projections and cortical gamma aperiodic components,as well as bidirectional primary motor cortex(M1)↔dorsolateral striatum gamma *** of PD128907(a selective dopamine D3 receptor agonist)induced dyskinesia and excessive gamma oscillations with a bidirectional M1↔dorsolateral striatum ***,administration of PG01037(a selective dopamine D3 receptor antagonist)attenuated dyskinesia,suppressed gamma oscillations and cortical gamma aperiodic components,and decreased gamma causality in the M1→dorsolateral striatum *** findings suggest that the dopamine D3 receptor plays a role in dyskinesia-related oscillatory activity,and that it has potential as a therapeutic target for levodopa-induced dyskinesia.

关键词： aperiodic components dopamine D3 receptor dorsolateral striatum functional connectivity gamma oscillations levodopa-induced-dyskinesia local field potentials neuromodulation Parkinson’s disease primary motor cortex

The potential mechanism and clinical application value of remote ischemic conditioning in stroke

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Neural Regeneration Research 2025年第6期20卷 1613-1627页

作者： Yajun Zhu Xiaoguo Li Xingwei Lei Liuyang Tang Daochen wen Bo Zeng Xiaofeng Zhang Zichao Huang Zongduo guo The First Affiliated Hospital of Chongqing Medical University ChongqingChina

Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.

关键词： Akt apoptosis autophagy cerebral perfusion cerebral vascular stenosis clinical transformation hemorrhagic stroke ischemic stroke neuroinflammation neuroprotection Notch1 PI3K remote ischemic conditioning stroke

Long-term corrosion behavior of HVOF sprayed FeCrSiBMn amorphous/nanocrystalline coating

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Transactions of Nonferrous Metals Society of China 2015年第4期25卷 1144-1150页

作者：秦玉娇吴玉萍张建峰郭文敏洪晟陈丽艳河海大学力学与材料学院金属材料与防护研究所南京210098

A FeCrSiBMn amorphous/nanocrystalline coating with 700 μm in thickness and 0.65% in porosity, was prepared by high velocity oxygen fuel(HVOF) spraying process. The long-term corrosion behavior of the FeCrSiBMn coating was evaluated by potentiodynamic polarization and electrochemical impedance spectroscopy(EIS) tests in a 3.5% NaCl solution with a hard chromium coating as a reference. The FeCrSiBMn coating exhibited higher corrosion potential and lower corrosion current density than the hard chromium coating. The pore resistance(Rp) and charge transfer resistance(Rct) of FeCrSiBMn coating were higher than those of the hard chromium coating. In addition, after immersion in the Na Cl solution for 28 d, only small pores in the FeCrSiBMn coating were observed. All the results indicated that the FeCrSiBMn coating held superior corrosion resistance to the hard chromium coating. This could be attributed to the dense structure, low porosity and amorphous/nanocrystalline phases of the FeCrSiBMn coating.

关键词： HVOF spraying coating Fe-based amorphous/nanocrystalline corrosion resistance