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Prohibitin(PHB)interacts with AKT in mitochondria to coordinately modulate sperm motility

Asian Journal of Andrology 2020年第6期22卷 583-589页

作者： Xiao-Hui Li Ran-Ran Chai Guo-Wu Chen Ling-Fei Zhang wen-jing tan-tai Hui-Juan Shi Patricia A Martin-DeLeon Wai-Sum O Hong Chen Department of Anatomy Histology and EmbryologyKey Laboratory of Medical Imaging Computing and Computer Assisted Intervention of ShanghaiSchool of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Ji'Ai Genetics and IVF Institute Obstetrics and Gynecology HospitalInstitute of Reproduction and DevelopmentFudan UniversityShanghai 200011China Key Lab of Reproduction Regulation of NPFPC-Shanghai Institute of Planned Parenthood Research Fudan University Reproduction and Development InstitutionShanghai 200032China Department of Biological Sciences University of DelawareNewarkDE 19716-2590USA School of Biomedical Sciences The University of Hong KongHong Kong SARChina

Prohibitin(PHB),an evoluti on arily con served mitochondrial inner membra ne protein,is highly expressed in cells that require strong mitoch on drial ***,we dem on strated that the deleti on of Phb in spermatocytes results in impaired mitochondrial *** addition,PHB expression in the mitochondrial sheath of human sperm has a significantly negative correlation with mitochondrial reactive oxygen species levels,but a positive one with mitochondrial membrane potential and sperm *** results suggest that mitochondrial PHB expression plays a role in sperm ***,the mechanism of PHB-mediated regulation of sperm motility remai ns unk ***,we dem on strate for the first time that PHB interacts with protei n kinase B(AKT)and exists in a complex with phospho-PHB(pT258)and phospho-AKT in the mitochondrial sheath of murine sperm,as determined using colocalization and coimmunoprecipitation *** blocking AKT activity using wortmannin(a phosphatidylinositol 3-kinase[PI3K]inhibitor),murine sperm have significantly(P<0.05)decreased levels of phospho-PHB(pT258)and the total and progressive ***,significantly(P<0.05)lower levels of phospho-PI3K P85 subunit a+γ(pY199 and pY46)and phospho-AKT(pS473;pT308)are found in sperm from infertile asthenospermic and oligoasthenospermic men compared with no rmospermic subjects,which suggest a reduced activity of the PI3K/AKT pathway in these in fertile ***,these sperm from infertile subjects also have a significantly(P<0.05)lower level of phospho-PHB(pT258).Collectively,our findings suggest that the interaction of PHB with AKT in the mitochondrial sheath is critical for sperm motility,where PHB phosphorylation(pT258)level and PI3K/AKT activity are key regulatory factors.

关键词： male in fertility prohibitin(PHB) protein kinase B(AKT) sperm motility

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Expression of PYCARD Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Primary Gout Patients with Different Chinese Medicine Syndromes

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Chinese Journal of Integrative Medicine 2018年第1期24卷 24-31页

作者： DANG Wan-tai XIE wen-guang ZHOU jing-guo School of Clinical Medicine Chengdu University of Traditional Chinese Medicine Institute of Rheumatology and Immunology Affiliated Hospital of North Sichuan Medical College

Objective： To study the expression level and role of apoptosis-associated speck-like protein containing a caspase recruitment domain （PYCARD） gene transcript variant mRNA in peripheral blood mononuclear cells （PBMCs） of primary gout （PG） patients with different Chinese medicine （CM） syndromes. Methods： The expressions of PYCARD gene transcript variant mRNA and interleukin-1β （IL-1β） mRNA in PBMCs were investigated in 96 PG patients with acute phase （APPG, 44 cases） and non-acute phase （NAPPG, 52 cases） and 30 healthy controls （HCs） by reverse transcription-polymerase chain reaction （PCR） and/or real-time quantitative PCR. PYCARD and nuclear factor-κB （p50） [NF-κB （p50）] protein was detected by Western blot in PBMCs respectively. IL-1β, IL-4 and IL-10 protein levels in plasma of HCs and PG patients were measured by enzyme-linked immuno sorbent assay. Results： The main CM syndromes in APPG patients were obstruction of dampness and heat syndrome （ODHS, 36.36%） and intermingled phlegm-blood stasis syndrome （IPBSS, 27.27%）, while in NAPPG patients were Pi （Spleen）-deficiency induced dampness syndrome （PDIDS, 40.38%） and qi-blood deficiency syndrome （QBDS, 26.92%）. It showed statistical significances of the expressions of PYCARD gene and its transcript variant mRNA, the protein of PYCARD and NF-κB （p50） and the plasma IL-1β, IL-4 and IL-10 in APPG, NAPPG, ODHS, IPBSS, PDIDS and QBDS groups, compared with the HC group respectively （P〈0.05 or P〈0.01）. There were also significant differences of mRNA expressions of PYCARD-1 and PYCARD-2 as well as protein expressions of IL-1β, IL-4 and IL-10 among the 4 CM syndromes groups （P〈0.05 or P〈0.01）. Correlation analysis showed positive correlation between the mRNA expressions of PYCARD-1 gene transcript variant and IL-1β in APPG patients （r=0.3088, P=0.0183）. Conclusion： PYCARD gene and its transcript variant may play a critical and regulative role in the inflammatory response of PG patients with different phases and CM syndromes.

关键词： Chinese medicine syndrome primary gout PYCARD gene transcript variant

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The Citron homology domain of MAP4Ks improves outcomes of traumatic brain injury

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Neural Regeneration Research 2025年第11期20卷 3233-3244页

作者： Xiaoling Zhong wenjiao tai Meng-Lu Liu Shuaipeng Ma Tianjin Shen Yuhua Zou Chun-Li Zhang Department of Molecular Biology University of Texas Southwestern Medical CenterDallasTXUSA Hamon Center for Regenerative Science and Medicine University of Texas Southwestern Medical CenterDallasTXUSA Peter O’Donnell Jr.Brain Institute University of Texas Southwestern Medical CenterDallasTXUSA

The mitogen-activated protein kinase kinase kinase kinases(MAP4Ks)signaling pathway plays a pivotal role in axonal regrowth and neuronal degeneration following *** targeting this pathway is beneficial to brain injury remains *** this study,we showed that adeno-associated virus-delivery of the Citron homology domain of MAP4Ks effectively reduces traumatic brain injury-induced reactive gliosis,tauopathy,lesion size,and behavioral *** inhibition of MAP4Ks replicated the ameliorative effects observed with expression of the Citron homology ***,the Citron homology domain acted as a dominant-negative mutant,impeding MAP4K-mediated phosphorylation of the dishevelled proteins and thereby controlling the Wnt/β-catenin *** findings implicate a therapeutic potential of targeting MAP4Ks to alleviate the detrimental effects of traumatic brain injury.

关键词： adeno-associated virus Citron homology Citron homology domain gene therapy mitogen-activated protein kinase kinase kinase kinases traumatic brain injury

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Repeatable adaptive keypoint detection via self-supervised learning

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Science China(Information Sciences) 2022年第11期65卷 123-147页

作者： Pei YAN Yihua tan Yuan tai National Key Laboratory of Science & Technology on Multi-spectral Information Processing School of Artificial Intelligence and Automation Huazhong University of Science and Technology

Keypoint-based matching is a fundamental technology for different computer vision tasks, in which keypoint detection is a crucial step and directly affects the entire performance. Based on deep learning approaches, the learning-based keypoint detectors have been significantly developed. To further improve the accuracy of high-level matching tasks, the extracted keypoints should provide more accurate point-to-point correspondences and maintain a uniform spatial distribution. Based on this idea, a self-supervised learning method of keypoint detection named repeatable adaptive point is proposed. This method consists of a selfsupervised objective and an optimization algorithm. The objective maximizes the repeatability measure with the sparsity constraint of keypoints. This sparsity constraint is formulated by combining the non-maximum suppression operation and the penalty function of keypoint number, which generally makes keypoints have a uniform spatial distribution. A novel approximate alternate optimization algorithm is proposed to maximize the above objective, whose convergence is proved in theory. The proposed detector is “adaptive” because the combinations of it and some existing descriptors can adapt to high-level matching tasks with a fast convergence speed. Specifically, the combinations of it and SuperPoint/HardNet descriptors achieve stateof-the-art accuracy on three high-level tasks based on image matching, namely homography estimation,camera pose estimation, and three-dimensional reconstruction. Furthermore, the proposed method converges faster on new scenes compared with the state-of-the-art method that jointly optimizes the detector and the descriptor.

关键词： keypoint detection convolutional neural network image matching self-supervised learning repeatable

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Variation of Sediment Concentration and Its Drivers Under Different Soil Management Systems

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Pedosphere 2010年第5期20卷 578-585页

作者： ZHANG wen-tai YU Dong-Sheng SHI Xue-Zheng tan Man-Zhi LIU Liu-Song State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 (China) College of Geography Sciences Nanjing Normal University Nanjing 210046 (China) Graduate University of the Chinese Academy of Sciences Beijing 100049 (China)

In order to prevent soil erosion in southern China,a study was performed to determine the drivers of sediment concentration variation using simulated rainfall and four soil management systems under field *** soil management systems,i.e.,forest and grass coverage(FG),forest coverage with disturbed soil surface(FD),contour tillage(CT) and downslope tillage(DT),were exposed to two rainfall intensities(40 and 54 mm h-1) using a portable rainfall *** drivers of sediment concentration variation were determined by the variations of runoff rate and sediment concentration as well as their *** effects of the four soil management systems in preventing water and soil losses were compared using runoff rates and sediment concentrations at steady *** runoff initial stage,sediment concentration variation was mainly driven by rainfall and *** degree of sediment concentration variation driven by flow varied with different soil management *** best relationships between runoff rate and sediment concentration were identified,i.e.,reciprocal(CT),quadratic(FG and FD) and exponential(DT).At steady state,runoff rates of the four soil management systems varied slightly,whereas their sediment concentrations varied *** and CT were recommended as the best soil management systems for preventing water and soil losses.

关键词： runoff rate runoff steady state simulated rainfall soil erosion

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A new potential risk factor in patients with erectile dysfunction and premature ejaculation： folate deficiency

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Asian Journal of Andrology 2014年第6期16卷 902-906页

作者： wen-Jie Yan Nan Yu tai-Lang Yin Yu-]ie Zou jing Yang Reproductive Medical Center Renmin Hospital of Wuhan University Wuhan China.

We investigated serum folic acid （FA） levels in patients with erectile dysfunction （ED） and/or premature ejaculation （PE）. Fasting serum samples were obtained from 42 patients with ED, 36 with PE, 25 ED patients with PE, and 30 healthy men; the mean intravaginal ejaculation latency time （IELT） was measured during a 4 weeks baseline period. Levels of sex hormones （follicle-stimulating hormone, luteinizing hormone, total testosterone）, homocysteine （Hcys）, and FA were measured using chemiluminescent immunoassays. The sexual functions of PE patients and normal control men were evaluated using the Chinese Index of Premature Ejaculation （CIPE）. The abridged Intemational Index of Erectile Function-5 （IIEF-5） questionnaire was used to gauge erectile quality for ED patients and for normal controls. Serum FA concentrations were lower in ED （7.61 ~ 3.97 ng ml-1）, PE （9.37 ＋ 3.40 ng ml-1）, and ED/PE （8.84 ＋ 4.28 ng m1-1） patients than in healthy men （12.23 ~ 5.76 ng ml-z, P〈 0.05）. No significant differences in sex hormone levels were found between patients with sexual dysfunction and healthy controls （P 〉 0.05）. There were positive correlations between serum FA concentrations and ClPE scores （r= 0.530, P〈 0.01）, IIEF-5 scores （r= 0.589, P〈 0.01）, and IELT （r= 0.445, P〈 0.01）; negative correlations with Hcys concentrations （r= -0.487, P〈 0.01） were found in all participants. These findings showed a strong relationship between serum FA levels and sexual dysfunction, possibly due to an effect of FA on the metabolism of nitric oxide, Hcys, and 5-hydroxytryptamine.

关键词： 5-hydroxytryptamine erectile dysfunction folic acid homocysteine nitric oxide premature ejaculation

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Diagnosis of chest pain with foregut symptoms in Chinese patients

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World Journal of Gastroenterology 2009年第6期15卷 742-747页

作者： Bo Deng Ru-wen Wang Yao-Guang Jiang Qun-You tan Xiang-Li Liao jing-Hai Zhou Yun-Ping Zhao tai-Qian Gong Zheng Ma Thoracic Surgery Department Institute of Surgery Research Daping Hospital The Third Military Medical University Chongqing 400042 China

AIM:To evaluate the diagnosis of chest pain with foregut symptoms in Chinese patients. METHODS:Esophageal manometric studies, 24-h introesophageal pH monitoring and 24-h electrocardiograms(Holter electrocardiography)were performed in 61 patients with chest pain. RESULTS:Thirty-nine patients were diagnosed with non-specific esophageal motility disorders(29 patients with abnormal gastroesophageal reflux and eight patients with myocardial ischemia).Five patients had diffuse spasm of the esophagus plus abnormal gastroesophageal reflux(two patients had concomitant myocardial ischemia),and one patient was diagnosed with nutcracker esophagus. CONCLUSION:The esophageal manometric studies, 24-h intra-esophageal pH monitoring and Holter electrocardiography are significant for the differential diagnosis of chest pain,particularly in patients with foregut *** cases of esophageal motility disorders,pathological gastroesophageal reflux may be a major cause of chest pain with non-specific esophageal motility *** of the esophageal smooth muscle might affect the heart-coronary smooth muscle,leading to myocardial ischemia.

关键词： Chest pain Esophageal manometric Twenty-four-hour intra-esophageal pH monitoring Holter electrocardiography

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Can Vitamin D supplementation be used as adjunctive treatment for oligozoospermia or asthenozoospermia accompanied with Vitamin D deficiency？

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Asian Journal of Andrology 2015年第1期17卷 165-167页

作者： wen-Jie Yan Nan Yu tai-Lang Yin Liu Liu jing Yang Reproductive Medical Center Renmin Hospital of Wuhan University Wuhan 430060Hubei China.

Dear Editor, We read the paper by Hammoud et al. in the issue of Asian Journal of Andrology with great interests. This paper revealed that sperm concentration, sperm progressive motility, sperm morphology, and total progressively motile sperm count were lower in men with 25（OH） D 〉 50 ng ml-1 when compared with men with 20 ng m1-1 -〈 25（OH） D 〈 50 ng ml-2. Total sperm count and total progressive motile sperm count were lower in men with 25（OH） D 〈 20 ng ml-1 when compared to men with 20 ng ml 1-〈 25（OH） D 〈 50 ng ml-1. Therefore, they draw the conclusion that serum Vitamin D （VD） levels at high and low levels can be negatively associated with semen parameters.

关键词： Can Vitamin supplementation adjunctive treatment

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Generalized Wigner Functions for Damped Systems in Deformation Quantization

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Communications in Theoretical Physics 2007年第2X期48卷 255-260页

作者： HENG tai-Hua jing Si-Cong Department of Modern Physics University of Science and Technology of China Hefei 230026 China

Quantization of damped systems usually gives rise to complex spectra and corresponding resonant states, which do not belong to the Hilbert space. Therefore, the standard form of calculating Wigner function （WF） does not work for these systems. In this paper we show that in order to let WF satisfy a ,-genvalue equation for the damped systems, one must modify its standard form slightly, and this modification exactly coincides with the results derived from a ＊-Exponential expansion in deformation quantization.

关键词： Wigner function damped system deformation quantization

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陆缘弧岩浆寂静期花岗质岩浆成因:以青藏高原东南缘腾冲地块晚白垩世花岗岩为例

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岩石学报 2025年第1期41卷 91-111页

作者：赵少伟方肖雨文泰赖绍聪裴先治李佐臣长安大学地球科学与资源学院西安710054 大陆动力学国家重点实验室西北大学地质学系西安710069

陆缘弧岩浆活动常具有幕式特征,展现出弧岩浆爆发和寂静交替的现象。虽然对其律动特征广泛研究,但目前对于岩浆寂静期的岩浆成因机制了解较少。本文通过对比新特提斯洋俯冲相关的陆缘弧岩浆分布特征,以新特提斯洋俯冲过程中陆源弧岩浆... 详细信息

陆缘弧岩浆活动常具有幕式特征,展现出弧岩浆爆发和寂静交替的现象。虽然对其律动特征广泛研究,但目前对于岩浆寂静期的岩浆成因机制了解较少。本文通过对比新特提斯洋俯冲相关的陆缘弧岩浆分布特征,以新特提斯洋俯冲过程中陆源弧岩浆寂静期的腾冲地块晚白垩世花岗质岩石为研究对象,探讨其形成过程,为寂静期岩浆的成因动力学机制提供重要约束。腾冲地块户撒地区二长花岗岩锆石U-Pb年龄为73Ma,形成于新特提斯洋俯冲陆缘弧晚白垩世(85~65Ma)的岩浆寂静期,为准铝质-弱过铝质和钙碱质花岗岩,具有富集的Nd-Hf同位素,其源岩为高黎贡山群正变质片麻岩。通过区域对比,晚白垩世(85~65Ma)新特提斯洋低角度俯冲相关的陆源弧应为挤压环境,腾冲地块同期花岗岩类应形成于加厚地壳背景下。通过与爆发期(55~50Ma)岩石组合、地化特征、锆饱和温度和斜长石环带特征的对比,本文认为腾冲地块晚白垩世花岗岩类是加厚地壳长期热积累诱发的下地壳片麻岩部分熔融的结果。因此,在幔源岩浆低通量的陆缘弧寂静期,通过加厚地壳长期热积累可以在陆缘弧局部形成长英质岩浆。

关键词：岩浆寂静期腾冲地块晚白垩世花岗岩

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