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Investigating the mechanisms of isochorismate synthase:An approach to improve salicylic acid synthesis and increase resistance to Fusarium head blight in wheat

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The Crop Journal 2024年第4期12卷 1054-1063页

作者： Ya-Zhou Zhang jie Man Dan Xu Lan wen Yinghui Li Mei Deng Qian-Tao Jiang Qiang Xu Guo-Yue Chen Yu-Ming wei State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina

Salicylic acid(SA),a vital endogenous hormone,plays a crucial role in plant growth and the response to abiotic and biotic *** synthase(ICS)and phenylalanine ammonia lyase(PAL)are critical rate-limiting enzymes for SA *** head blight(FHB)seriously threatens the safety of wheat production,but increasing the content of SA can enhance FHB ***,the pathway of SA synthesis and regulation in wheat remains *** this study,three wheat ICS(TaICSA,TaICSB,and TaICSD)were identified,and their functions were validated in vitro for isomerizing chorismate to *** mutation of one or two homoeoalleles of TaICSA,TaICSB,and TaICSD in the wheat variety‘Cadenza’reduced SA levels under ultraviolet treatment and Fusarium graminearum infection,further enhancing sensitivity to *** of TaICSA can significantly enhance SA levels and resistance to *** further study SA synthesis pathways in wheat and avoid interference with pathogenicity related genes,the leaves of wild-type Cadenza and different TaICS mutant lines were subjected to ultraviolet treatment for transcriptomic *** results showed that 37 PALs might be involved in endogenous SA synthesis,and 82 WRKY and MYB family transcription factors may regulate the expression of ICS and *** results were further confirmed by *** conclusion,this study expands our knowledge of SA biosynthesis and identifies TaICSA,as well as several additional candidate genes that encode transcription factors for regulating endogenous SA levels,as part of an efficient strategy for enhancing FHB resistance in wheat.

关键词： Salicylic acid Chorismate Isochorismate RNA-seq Phenylalamine ammonia lyase

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Transcriptomic and epigenomic landscapes of muscle growth during the postnatal period of broilers

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Journal of Animal Science and Biotechnology 2024年第5期15卷 1851-1865页

作者： Shuang Gu Qiang Huang Yuchen jie Congjiao Sun Chaoliang wen Ning Yang State Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding China Agricultural UniversityBeijing 100193China National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsDepartment of Animal Genetics and BreedingCollege of Animal Science and Technology China Agricultural UniversityBeijing 100193China. Sanya Institute of China Agricultural University Hainan 572025China

Background Broilers stand out as one of the fastest-growing livestock globally,making a substantial contribution to animal meat ***,the molecular and epigenetic mechanisms underlying the rapid growth and development of broiler chickens are still *** study aims to explore muscle development patterns and regulatory networks during the postnatal rapid growth phase of fast-growing *** measured the growth performance of Cornish(CC)and White Plymouth Rock(RR)over a 42-d *** muscle samples from both CC and RR were randomly collected at day 21 after hatching(D21)and D42 for RNA-seq and ATAC-seq library *** The consistent increase in body weight and pectoral muscle weight across both breeds was observed as they matured,with CC outpacing RR in terms of weight at each stage of *** expression analysis identified 398 and 1,129 genes in the two dimensions of breeds and ages,respectively.A total of 75,149 ATAC-seq peaks were annotated in promoter,exon,intron and intergenic regions,with a higher number of peaks in the promoter and intronic *** age-biased genes and breed-biased genes of RNA-seq were combined with the ATAC-seq data for subsequent *** results spotlighted the upregulation of ACTC1 and FDPS at D21,which were primarily associated with muscle structure development by gene cluster ***,a noteworthy upregulation of MUSTN1,FOS and TGFB3 was spotted in broiler chickens at D42,which were involved in cell differentiation and muscle regeneration after injury,suggesting a regulatory role of muscle growth and *** This work provided a regulatory network of postnatal broiler chickens and revealed ACTC1 and MUSTN1 as the key responsible for muscle development and *** findings highlight that rapid growth in broiler chickens triggers ongoing muscle damage and subsequent *** findings provide a foundation for future research to i

关键词： ATAC-seq Broiler Pectoral muscle development Rapid growth RNA-seq

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Eccrine porocarcinoma in the tempus of an elderly woman:A case report

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World Journal of Clinical Cases 2024年第8期12卷 1523-1529页

作者： Zhi-wei Wu wen-jie Zhu Shan Huang Qi Tan Cong You Dian-Gui Hu Long-Nian Li Department of Dermatology First Affiliated Hospital of Gannan Medical UniversityGanzhou 341000Jiangxi ProvinceChina Department of Laser and Cosmetic Dermatology Ganzhou Dermatology HospitalGanzhou 341000Jiangxi ProvinceChina Department of Dermatology Tongxiang Dermatology HospitalJiaxing 314000Zhejiang ProvinceChina Department of Infectious Diseases The Fifth people’s Hospital of GanzhouGanzhou 341000Jiangxi ProvinceChina

BACKGROUND Eccrine porocarcinoma(EPC)is a rare skin tumor that mainly affects the elderly *** often present with slow growth and a good *** are usually distinguished from other skin tumors using histopathology and ***,surgical management alone may be inadequate if the tumor has ***,currently,surgical resection is the most commonly used treatment *** SUMMARY A seventy-four-year-old woman presented with a slow-growing nodule in her left temporal area,with no obvious itching or pain,for more than four *** examination showed small columnar and short spindle-shaped cells;thus,basal cell carcinoma was ***,immunohistochemical analysis revealed the expression of cytokeratin 5/6,p63 protein,p16 protein,and Ki-67 antigen(40%),and EPC was taken into *** skin biopsy was repeated,and hematoxylin and eosin staining revealed ductal differentiation in some ***,the patient was diagnosed with EPC,and Mohs micrographic surgery was *** adapted follow-up visits in a year and not found any recurrence of *** This case report emphasizes the diagnosis and differentiation of EPC.

关键词： Eccrine porocarcinoma Sweat gland carcinoma Histopathology Immunohistochemistry Prognosis Case report

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Sludge composition and characteristics shaped microbial community and further determined process performance: A study on full-scale thermal hydrolysis-anaerobic digestion processes

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Journal of Environmental Sciences 2024年第3期137卷 96-107页

作者： wei Liu Chenxiang Sun wei Li Tianle Li Zhan Chen Jiawei Wang Zhengran Ren Xianghua wen School of Environment Tsinghua UniversityBeijing 100084China Jinneng Holding Group DatongShanxi 037000China Research and Development Center Beijing Drainage Group Co.Ltd.Beijing 100124China Beijing Dabeinong Science and Technology Group Co. Ltd.Beijing 100080China

Anaerobic digestion (AD) with thermal hydrolysis (TH) pretreatment is a promising process for excess sludge treatment,while there lacks of the knowledge from full-scale process about the impact of sludge composition and characteristics on microbial community and *** sludge physiochemical indices,microbial community and performance data of four full-scale TH-AD plants were characterized,and their relationships was *** four plants were operated under almost similar total organic loading rate (OLR)but their methanogenesis performance differentiate into two groups,namely superior group(SupG) and the inferior group (Inf G).In both groups,TH effectively solubilized particulate organic compounds,meanwhile raised the ammonia nitrogen (NH_(4)^(+)-N) and volatile fatty acid (VFA) *** with the Sup G,thermal hydrolyzed sludge of Inf G had higher level of VFAs,NH_(4)^(+)-N and total chemical oxygen demand (t COD),which showed higher inhibition effect on microbes,leading to a community with lower diversity,lower abundance of carbohydrate degrading functional guild,higher protein degrading one,and methanogens that adapted to limited substrates,and further declined the methane production ***,it was recommended that OLR alone was not sufficient for controlling the system in design and operation,the concentration of VFAs,NH_(4)^(+)-N and t COD should be equally *** higher concentration,together with the higher abundance of Defluviitoga and Proteiniphilum were recommended as indicators for inferior running *** results proposed that microbial communities played a role of bridge between environmental factors and performance,provided implications for engineering ecology and operational regulation for full-scale sludge TH-AD process.

关键词： Thermal hydrolysis Anaerobic digestion Microbial community Full-scale

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Psychoactive Substance Use and the Impact on the AIDS Epidemic Among the MSM Population:a Meta-Analysis—Worldwide,2014-2023

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China CDC weekly 2024年第42期6卷 1086-1092,I0001-I0006页

作者： Donghang Luo jie Xu wei Luo National Center for AIDS/STD Control&Prevention Chinese Center for Diseases Control and PreventionBeijingChina

What is already known about this topic?There is a correlation between psychoactive substance use and heightened human immunodeficiency virus(HIV)susceptibility in men who have sex with men(MSM)populations,although the precise impact on transmission risk remains *** is added by this report?In MSM populations,rush poppers and amphetamines are widely used,with rates of 10.91%and 9.97%.In China,rush poppers usage is notably higher at 47.26%.For other regions and coutries,amphetamines(12.86%),rush poppers(3.62%),and GHB(3.34%)are most ***,17%of MSM use psychoactive substances,with higher HIV risk from amphetamines[Odds ratio(OR)3.06]compared to rush poppers(OR 1.51)and general substances(OR 1.76).What are the implications for public health practice?This study supports the idea that targeting specific regions and types of psychoactive substances for reduction among MSM populations can help decrease HIV transmission as well as promote prevention strategies.

关键词： prevention precise Impact

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A N-CoSe/CoSe_(2)-C@Cu hierarchical architecture as a current collector-integrated anode for potassium-ion batteries

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Rare Metals 2024年第8期43卷 3702-3712页

作者： Zi-jie Mu Yan-Jun Gao wen-Shuai Dong Zong-You Li Qing-Yi Song Han-Jiao Huang Li-Dong Xing Jian-Guo Zhang wei Wang Qi-Yao Yu State Key Laboratory of Explosion Science and Technology School of Mechatronical EngineeringBeijing Institute of TechnologyBeijing 100081China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

The highly reversible insertion/extraction of large-radius K^+into electrode materials remains a tough goal,especially for con version-type ***,we design a current collector-integrated electrode(N-CoSe/CoSe_(2)-C@Cu) as an advanced anode for potassium-ion battery(PIBs).The conductive CoSe/CoSe_(2) heterojunction with rich Se vacancy defects,conductive sp^2 N-doped carbon layer,and the elastic copper foil matrix can greatly accelerate the electron transfer and enhance the structural ***,the well-designed N-CoSe/CoSe_(2)-C@Cu current collector-integrated electrode displays enhanced potassium storage performance with regard to a high capacity(325.1 mAh·g^(-1) at 0.1 A·g^(-1) after 200cycles),an exceptional rate capability(223.5 mAh·g^(-1) at2000 mA·g^(-1)),and an extraordinary long-term cycle stability(a capacity fading of only 0.019% per cycle over1200 cycles at 2000 mA·g^(-1)).Impressively,ex situ scanning electron microscopy(SEM) characterizations prove that the elastic structure of copper foil is merged into the cleverly designed N-CoSe/CoSe_(2)-C@Cu heterostructure,which buffers the deformation of structure and volume and greatly promotes the cycle life during the potassium/depotassium process.

关键词： Potassium-ion battery Integrated collector electrode CoSe CoSe_2 Heterojunction Copper foil matrix N-doped carbon

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Infrared microspectroscopy beamline BL06B at SSRF

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Nuclear Science and Techniques 2024年第1期35卷 10-17页

作者： Te Ji Hua-Chun Zhu wei-wei Peng jie Wang Hong-wei Zhao Ai-Guo Li Min Chen Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China

The infrared microspectroscopy beamline(BL06B) is a phase Ⅱ beamline project at the Shanghai Synchrotron Radiation Facility(SSRF). The construction and optical alignment of BL06B were completed by the end of 2020. By 2021, it became accessible to users. The synchrotron radiation infrared(SRIR) source included edge radiation(ER) and bending magnet radiation(BMR). The extracted angles in the horizontal and vertical directions were 40 and 20 mrad, respectively. The photon flux, spectral resolution, and focused spot size were measured at the BL06B endstation, and the experimental results were consistent with theoretical calculations. SRIR light has a small divergence angle, high brightness, and a wide wavelength range. As a source of IR microscopy, it can easily focus on a diffraction-limited spatial resolution with a high signal-to-noise ratio(SNR). The BL06B endstation can be applied in a wide range of research fields, including materials, chemistry, biology, geophysics, and pharmacology.

关键词： Synchrotron radiation Infrared beamline Infrared microspectroscopy

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Fluorescent carbon dots synthesized in solid phase and air for application in LEDs

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Nano Research 2024年第9期17卷 8495-8503页

作者： wei-jie Zheng Zhao-Nan Sun Yi-Ming Wang Huan-Ming Xiong Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Fudan UniversityShanghai 200438China

Carbon dots(CDs)have been widely adopted as optical materials because of their excellent luminescent ***,most of the reported synthetic methods are conducted in solvents,especially hydrothermal/solvothermal reactions,leading to intractable problems such as toxic and flammable solvents,complex and inseparable by-products,and dangerously high pressures and ***-phase synthesis of CDs in air is an effective solution to overcome the above issues,but solid reactions always result in uncontrolled growth and agglomeration of *** this study,some inorganic salts are selected as catalysts for synthesizing CDs in solid states and air,which also play as dispersants to hinder CDs *** the meantime,some aromatic derivatives containing hydroxyl and amino groups are chosen as carbon sources,ground with the optimized catalyst,and then heated together in *** production yields are affected by the reaction time and reactant ratio,while the graphitization degrees of the CDs are determined by the reaction *** IG/ID value of their Raman spectra increases from 0.59 to 0.85,and the particle size decreases from 2.5 to 1.4 nm when the synthesis temperature is increased from 200 to 280℃.The as-prepared CDs show emission peaks ranging from 366 to 606 nm,with the photoluminescence(PL)quantum yield up to 53%.Their emission color variation mainly results from different carbon sources,which can be ascribed to the differences in the element composition,functional groups,and graphitic nitrogen content of these *** dispersing CDs of different concentrations into polyvinyl alcohol(PVA)and combining them with blue LEDs,cold,standard,and warm white light emitting devices(WLEDs)are prepared,with a color rendering index(CRI)up to *** the as-prepared CDs have antioxidant ability at high temperature,the as-prepared WLEDs have long lifespans,remaining the effective white luminescence after 72 h continuous work.

关键词： carbon dot solid-phase synthesis white LED photoluminescence inorganic salt catalysis

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How interfacial electron-donating defects influence the structure and charge of gold nanoparticles on TiO_(2)support

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Nano Research 2024年第7期17卷 5965-5974页

作者： Guang-jie Xia Yu Fu wei Cao Jun Li Yang-Gang Wang Department of Chemistry and Guangdong Provincial Key Laboratory of Catalysis Southern University of Science and TechnologyShenzhen 518055China School of Physical Sciences Great Bay UniversityDongguan 523000China Department of Chemistry Tsinghua UniversityBeijing 100084China

The reduction degree of TiO_(2)support is critical to the performances of metal *** many previous theoretical calculations,only the bridge oxygen vacancy(Ov)was considered as the electron-donating defect on reduced rutile TiO_(2)(r-TiO_(2−x))***,titanium adatoms(Tiad.),oxidized titanium islands(***),and acid hydroxyls(ObrH)also exist at the metal/support *** conducting density functional theory(DFT)calculations and ab initio molecular dynamics(AIMD)simulations,we compared r-TiO_(2−x)surfaces with Ov,Tiad.,***,and ObrH sites loaded with Au nanoparticles(NPs).The results showed the Au NPs were oxygen-phobic but titanium-philic,resulting in wetting of Ov and *** short contact with *** and *** Bader charges of Au NPs(QM)showed a good linear relationship with the ideal number of donating electrons(Ne)from the defective sites(QM=−KeNe+QM,S),demonstrating the intrinsic electron allocation at the *** Ov,Tiad.,and *** exhibited similar slopes(Ke),relatively steeper than that of *** means in the scope of Au NP charge state,the *** *** have a close electron-donating ability with Ov,but the ObrH donates relatively fewer *** linear relationship can be extended approximately to other *** higher the metal work function,the steeper the Ke for easier electron donation from defective *** stronger the metal oxygen affinity,the more positive the intercept(QM,S).That explains the easy generation of metallic or negative Pt and Au NPs on r-TiO_(2−x),but hard for Cu and Zn in *** provides theoretical guidance for regulating the charge of metal NPs over TiO_(2−x)supports.

关键词： metal-support interaction titanium oxide oxygen vacancy surface hydroxyl titanium adatom

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Single-cluster electronics using metallic clusters:Fabrications,regulations,and applications

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Nano Research 2024年第1期17卷 65-78页

作者： Caiyun wei wei Xu Shurui Ji Ruiyun Huang Junyang Liu wenqiu Su jie Bai Jiale Huang wenjing Hong State Key Laboratory of Physical Chemistry of Solid Surfaces National Engineering Research Centre of Chemicals for Electronic ManufacturingCollege of Chemistry and Chemical Engineering&Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM)Xiamen UniversityXiamen 361005China

Metallic clusters,ranging from 1 to 2 nm in size,have emerged as promising candidates for creating nanoelectronic devices at the single-cluster *** the intermediate quantum properties between metals and semiconductors,these metallic clusters offer an alternative pathway to silicon-based electronics and organic molecules for miniaturized electronics with dimensions below 5 *** progress has been made in studies of single-cluster electronic ***,a clear guide for selecting,synthesizing,and fabricating functional single-cluster electronic devices is still *** review article provides a comprehensive overview of single-cluster electronic devices,including the mechanisms of electron transport,the fabrication of devices,and the regulations of electron transport ***,we discuss the challenges and future directions for single-cluster electronic devices and their potential applications.

关键词： single-cluster junctions electronic properties electron transport metallic clusters structure regulation

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