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A nonsensory odorant-binding protein plays an important role in the larval development and adult mating of Spodoptera frugiperda

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Insect Science 2023年第5期30卷 1325-1336页

作者： wei-Kang Han Feng-Xian Tang Na Yu Yi-Xi Zhang Ze-wen Liu Key Laboratory of Integrated Management of Crop Diseases and Pests(Ministry of Education) College of Plant ProtectionNanjing Agricultural UniversityNanjingChina

Odorant-binding proteins (OBPs) play key roles in the perception of semiochemicals in insects. Several OBPs in insect olfactory systems have been functionally characterized, and they provide excellent targets for pest control. The functions of some OBPs that are highly expressed in the nonsensory organs of insects remain unclear. Here, the physiological function of an OBP (OBP27) that was highly expressed in the nonsensory organs of Spodoptera frugiperda was studied. OBP27 was nested within the Plus-C cluster according to phylogenetic analysis. The transcription of OBP27 steadily increased throughout the development of S. frugiperda, and transcripts of this gene were abundant in the fat body and male reproductive organs. An OBP27 knockout strain with an early frameshift mutation was obtained using the clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9 (Cas9) system. The development time of OBP27^(−/−) larvae was significantly longer than that of other larvae. Both male and female OBP27^(−/−) pupae weighed significantly less than wild-type (WT) pupae. In crosses of OBP27^(−/−) males or females, the mating rate was lower and the mating duration was longer for OBP27^(−/−) male–WT female pairs than for WT–WT pairs. By contrast, the mating rate, hatching rate, and number of eggs of OBP27^(−/−) female–WT male pairs and WT–WT pairs were similar. These findings indicate that OBP27 plays an important role in the larval development and mating process in male adults. Generally, our findings provide new insights into the physiological roles of nonsensory OBPs.

关键词： CRISPR/Cas9 nonsensory OBP reproduction Spodoptera frugiperda

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湘西北地区雪茄烟种植气候适宜性评价

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安徽农业科学 2024年第1期52卷 207-212页

作者：郭世杰王会青汪薇田金艳戴曦王振华湖南省烟草公司张家界市公司湖南张家界427000 河南农业大学烟草行业烟草栽培重点实验室河南郑州450002

为了明确湘西北地区雪茄烟种植气候适宜性,以7个县1991-2020年的气候观测数据为基础资料,选取10项关键气候因子,运用模糊数学隶属函数和主成分分析法对湘西北地区的气候适宜性进行综合评价。结果表明,湘西北地区种植雪茄烟的气候适宜性... 详细信息

为了明确湘西北地区雪茄烟种植气候适宜性,以7个县1991-2020年的气候观测数据为基础资料,选取10项关键气候因子,运用模糊数学隶属函数和主成分分析法对湘西北地区的气候适宜性进行综合评价。结果表明,湘西北地区种植雪茄烟的气候适宜性由高到低依次为慈利县、麻阳县、靖州县、龙山县、凤凰县、桑植县、永顺县。湘西北各县均存在5月初-6月初降雨量和7月初-8月底降雨量偏多、5月初-8月底日照时数偏低的情况,这是雪茄烟生产种植的障碍因子。

关键词：雪茄烟气候适宜性温度降雨量湿度日照时数湘西北地区

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Development of a poly(arylene sulfide sulfone)antibacterial electrospun film as a skin wound dressing application

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Journal of Innovative Optical Health Sciences 2022年第2期15卷 32-41页

作者： Xuzhi Lang Zhurong Tang Zhimei wei Xiaojun Wang Shengru Long Gang Zhang jie Yang Qingyu Lin Qingdao Benzo Chemical Company Limited Qingdao 266300 P.R.China Institute of Life Sciences Chongqing Medical University Chongqing 400016P.R.China Institute of Materials Science and Technology Analytical&Testing Center Sichuan University Chengdu 610065P.R.China State Key Laboratory of Polymer Materials Engineering(Sichuan University) Chengdu 610065P.R.China School of Mechanical Engineering Research Center of Analytical Instrumentation Sichuan University Chengdu 610065P.R.China

Tissue engineering has become a hot issue for skin wound healing because it can be used as an alternative treatment to traditional *** films have been widely used due to their excellent *** this work,an organic/inorganic composite poly(arylene sulfide sulfone)/ZnO/graphene oxide(PASS/ZnO/GO)nanofibrous film was fabricated with the ZnO nano-particles blending in an electrospun solution and post-treated with the GO *** optimal PASS/ZnO/GO nanofibrous film was prepared by 2%ZnO nanoparticles,3.0g/mL PASS electrospun solution,and 1%GO dispersion *** morphology,hydrophilicity,mechanical property,and cytotoxicity of the PASS/ZnO/GO nanofibrous film were character-ized by using scanning electron microscopy,transmission electron microscope,water contact angle,tensile testing,and a Live/Dead cell staining *** is founded that the PASS/ZnO/GO nanofibrous film has outstanding mechanical properties and no ***,the PASS/ZnO/GO nanofibrous film exhibits excellent antibacterial activity to both Escherichia coli and Staphylococcus *** all,this high mechanical property in the non-toxic and antibacterial nanofibrous film will have excellent application prospects in skin wound dressing.

关键词： Graphene oxide electrospinning antibacterial skin wound dressing

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奥拉西坦联合丙戊酸镁对老年脑卒中后癫痫的治疗效果及氧化应激反应的作用研究

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中国现代医学杂志 2024年第3期34卷 8-12页

作者：刘鹏文立杨高国杰李航毛森林黑龙江中医药大学附属第一医院针灸六科黑龙江哈尔滨150040 黑龙江中医药大学黑龙江哈尔滨150040 哈尔滨医科大学附属第二医院神经内科黑龙江哈尔滨150001

目的探究奥拉西坦联合丙戊酸镁对老年脑卒中后癫痫的治疗效果及氧化应激反应的作用。方法选取2021年8月—2022年9月黑龙江中医药大学附属第一医院收治的92例老年脑卒中后癫痫患者,随机分为研究组与对照组,每组46例。对照组口服丙戊酸镁... 详细信息

目的探究奥拉西坦联合丙戊酸镁对老年脑卒中后癫痫的治疗效果及氧化应激反应的作用。方法选取2021年8月—2022年9月黑龙江中医药大学附属第一医院收治的92例老年脑卒中后癫痫患者,随机分为研究组与对照组,每组46例。对照组口服丙戊酸镁缓释片治疗,研究组在对照组基础联合奥拉西坦治疗,维持治疗3个月。比较两组患者治疗前后癫痫发作频率、单次癫痫发作持续时间、日常活动能力、认知水平、氧化应激反应、药物毒性及随访6个月的复发结局。结果治疗前后研究组癫痫发作频率差值、单次癫痫发作持续时间差值、日常活动能力评分差值、韦氏智力量表中国修订版评分差值、血清超氧化物歧化酶差值、血清脂质过氧化物差值均高于对照组(P0.05)。研究组6个月的复发率低于对照组(P<0.05)。结论奥拉西坦联合丙戊酸镁治疗老年脑卒中后癫痫,有助于提升抗癫痫效果,改善患者认知功能,减轻氧化应激损伤,且安全性良好。

关键词：脑卒中癫痫奥拉西坦丙戊酸镁氧化应激

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Novel green-fruit detection algorithm based on D2D framework

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International Journal of Agricultural and Biological Engineering 2022年第1期15卷 251-259,F0003页

作者： Jinmeng wei Yanhui Ding jie Liu Muhammad Zakir Ullah Xiang Yin weikuan Jia School of Information Science and Engineering Shandong Normal UniversityJinan 250358China School of Agricultural Engineering and Food Science Shandong University of TechnologyZibo 255000ShandongChina Key Laboratory of Facility Agriculture Measurement and Control Technology and Equipment of Machinery Industry Zhenjiang 212013JiangsuChina

In the complex orchard environment,the efficient and accurate detection of object fruit is the basic requirement to realize the orchard yield measurement and automatic *** it is hard to differentiate between the object fruits and the background because of the similar color,and it is challenging due to the ambient light and camera angle by which the photos have been *** problems make it hard to detect green fruits in orchard *** this study,a two-stage dense to detection framework(D2D)was proposed to detect green fruits in orchard *** proposed model was based on multi-scale feature extraction of target fruit by using feature pyramid networks MobileNetV2+FPN structure and generated region proposal of target fruit by using Region Proposal Network(RPN)*** the regression branch,the offset of each local feature was calculated,and the positive and negative samples of the region proposals were predicted by a binary mask prediction to reduce the interference of the background to the prediction *** the classification branch,features were extracted from each sub-region of the region proposal,and features with distinguishing information were obtained through adaptive weighted pooling to achieve accurate *** new proposed model adopted an anchor-free frame design,which improves the generalization ability,makes the model more robust,and reduces the storage *** experimental results of persimmon and green apple datasets show that the new model has the best detection performance,which can provide theoretical reference for other green object detection.

关键词： green-fruit detection D2D framework automatic harvesting MobileNetV2+FPN binary mask prediction anchor-free

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补肾调经汤联合人重组干扰素-α-2b对子宫内膜异位症体液免疫、CA125、EMAb水平的影响

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广州中医药大学学报 2024年第6期41卷 1472-1478页

作者：康晓蓓杨宏洁杜秀芳付昌娜杨涛孙伟伟石家庄市妇幼保健院河北石家庄050006 中国中医科学院广安门医院北京100053

【目的】探析补肾调经汤(在《景岳全书》大营煎方的基础上加味而成)联合人重组干扰素-α-2b治疗子宫内膜异位症(endometriosis,EMs)疗效及其对体液免疫、血清糖类抗原125(carbohydrate antigen,CA125)、抗子宫内膜抗体(antiendometrial ... 详细信息

【目的】探析补肾调经汤(在《景岳全书》大营煎方的基础上加味而成)联合人重组干扰素-α-2b治疗子宫内膜异位症(endometriosis,EMs)疗效及其对体液免疫、血清糖类抗原125(carbohydrate antigen,CA125)、抗子宫内膜抗体(antiendometrial antibody,EMAb)水平的影响。【方法】将86例EMs肾虚血瘀证患者随机分为观察组和对照组,每组各43例。对照组单纯给予人重组干扰素-α-2b治疗,观察组给予补肾调经汤联合人重组干扰素-α-2b治疗,疗程为1个月。观察2组患者治疗前后体液免疫因子[免疫球蛋白A(immunoglobulin A,IgA)、补体3(complement 3,C3)、补体4(complement 4,C4)]、子宫内膜异位包块直径及病理相关因子(CA125、EMAb)水平的变化情况,并评价2组患者的临床疗效和安全性。【结果】(1)治疗1个月后,观察组的总有效率为97.67%(42/43),对照组为81.40%(35/43),组间比较(χ2检验),观察组的疗效明显优于对照组(P0.05)。【结论】补肾调经汤联合人重组干扰素-α-2b治疗EMs肾虚血瘀证患者临床疗效显著,可有效改善患者体液免疫因子及血清CA125、EMAb水平,缩小子宫内膜异位包块,且具有较高的安全性。

关键词：子宫内膜异位症肾虚血瘀证补肾调经汤大营煎方人重组干扰素-α-2b 体液免疫糖类抗原125(CA125) 抗子宫内膜抗体(EMAb)

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基于百度试验法和窑干试验法优化火力楠木材干燥基准

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陕西林业科技 2024年第3期52卷 59-65页

作者：谭长强韦鹏练文新苏子梅申文辉黄腾华广西壮族自治区林业科学研究院广西优良用材林资源培育重点实验室南宁530002 广西大学南宁530004

为充分了解和合理利用世界珍稀优良树种、我国南方主要造林用材树种火力楠木材特性,给木材干燥工业生产提供较为科学和完善的指导性干燥基准,以48 a生人工林火力楠木材为研究对象,采用百度试验法制定初步的干燥基准和木材干燥箱,对火力... 详细信息

为充分了解和合理利用世界珍稀优良树种、我国南方主要造林用材树种火力楠木材特性,给木材干燥工业生产提供较为科学和完善的指导性干燥基准,以48 a生人工林火力楠木材为研究对象,采用百度试验法制定初步的干燥基准和木材干燥箱,对火力楠木材进行小试窑干试验并优化干燥基准,测定并分析火力楠木材干燥缺陷,结果表明:火力楠木材的干燥缺陷主要表现为初期开裂、扭曲和截面变形,无内裂,初期开裂为二级,扭曲特性为二级,内裂和截面变形均为一级;初期开裂和扭曲程度均为中等,截面变形程度较轻,不易产生内裂等严重的干燥缺陷;干燥速度为三级,干燥速度中等,属于容易干燥的树种。小试窑干工艺基本条件为:初期干燥温度为60~70℃,初期干湿球温度差为3~7℃,末期干燥温度为80~85℃。该条件下,火力楠木材干燥过程中未出现内裂和皱缩,平均最终含水率为7.9%、厚度上的含水率偏差为2.1%,干燥均匀度为0.6%,残余应力为1.4%,所有干燥质量指标均达到国家标准中锯材干燥质量等级的一级要求。为厚度为25~30 mm。

关键词：火力楠百度试验法窑干试验干燥质量干燥基准

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制动盘黑斑缺陷的成因分析与防止措施

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现代铸铁 2024年第2期44卷 30-34页

作者：林忠军王文胜王聪王海杰烟台胜地汽车零部件制造有限公司山东烟台264006

介绍了实心制动盘和通风制动盘铸件的结构,铸件在加工后发现在制动面根部出现黑斑缺陷,其外观形貌和出现部位类似,仅通过宏观形貌很难区分。利用EVO18钨丝灯扫描电镜、OXFORD电制冷能谱、ZEISS金相显微镜对缺陷的微观形貌进行检验对比,... 详细信息

介绍了实心制动盘和通风制动盘铸件的结构,铸件在加工后发现在制动面根部出现黑斑缺陷,其外观形貌和出现部位类似,仅通过宏观形貌很难区分。利用EVO18钨丝灯扫描电镜、OXFORD电制冷能谱、ZEISS金相显微镜对缺陷的微观形貌进行检验对比,发现两种缺陷存在明显差别,结合形成机理,认为两种缺陷分别为缩松和石墨粗大。最后通过增加冒口颈模数,增大冒口补缩通道,解决了实心制动盘的缩松问题;通过在安装面根部以及摩擦面与安装面之间铸出凹槽,减少局部热节,解决了石墨粗大问题。

关键词：灰铸铁制动盘缩松石墨粗大

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Pressure induced color change and evolution of metallic behavior in nitrogen-doped lutetium hydride

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Science China(Physics,Mechanics & Astronomy) 2023年第8期66卷 125-130页

作者： Ying-jie Zhang Xue Ming Qing Li Xiyu Zhu Bo Zheng Yuecong Liu Chengping He Huan Yang Hai-Hu wen National Laboratory of Solid State Microstructures and Department of Physics Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China

By applying pressures up to 42 GPa on the nitrogen-doped lutetium hydride(LuH2_(±x)N_(y)),we have found a gradual change of color from dark-blue to pink-violet in the pressure region of about 12 to 21 *** temperature dependence of resistivity under pressures up to 50.5 GPa shows progressively optimized metallic behavior with ***,in the pressure region for the color change,a clear decrease of resistivity is observed with the increase of pressure,which is accompanied by a clear increase of the residual resistivity ratio(RRR).Fitting to the low temperature resistivity gives exponents of about 2,suggesting a Fermi-liquid behavior in the low temperature *** general behavior in a wide temperature region suggests that the electron-phonon scattering is still the dominant *** magnetoresistance up to 9 T in the state under a pressure of 50.5 GPa shows an almost negligible effect,which suggests that the electric conduction in the pink-violet state is dominated by a single *** is highly desired to have theoretical efforts in understanding the evolution of color and resistivity in this interesting system.

关键词： nitrogen-doped lutetium hydride room temperature superconductivity high-pressure technique color change

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Regulation of seed traits in soybean

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aBIOTECH 2023年第4期4卷 372-385页

作者： Yang Hu Yue Liu Jun-jie wei Wan-Ke Zhang Shou-Yi Chen Jin-Song Zhang State Key Lab of Plant Genomics Institute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China

Soybean (Glycine max) is an essential economic crop that provides vegetative oil and protein forhumans, worldwide. Increasing soybean yield as well as improving seed quality is of great *** weight/size, oil and protein content are the three major traits determining seed quality, and seedweight also influences soybean yield. In recent years, the availability of soybean omics data and thedevelopment of related techniques have paved the way for better research on soybean functionalgenomics, providing a comprehensive understanding of gene functions. This review summarizes theregulatory genes that influence seed size/weight, oil content and protein content in soybean. We alsoprovided a general overview of the pleiotropic effect for the genes in controlling seed traits andenvironmental stresses. Ultimately, it is expected that this review will be beneficial in breedingimproved traits in soybean.

关键词： Soybean Seed weight and size Oil Protein

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