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Molecular Cloning and Bacterial Expression of Germacrene A Synthase cDNA from Crepidiastrum sonchifolium

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Chemical Research in Chinese Universities 2006年第5期22卷 606-611页

作者： REN jie LIU Yan-qiu YANG Li ni Rui YOU Song School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016 P. R. China

Germacrene A synthase（GAS） catalyzes the biosynthesis of germacrene A, which is a key precursor for sesquiterpone lactones. Cloning of a novel full-length cDNA encoding GAS from the medicinal plant Crepidiastrum sonchifolium（dosignated CsGAS） is reported in this study. The cDNA is 1837 bp long and contains a 1680-bp open reading frame encoding a 559 amino-acid protein. The functional expression of the cDNA in Escherichia coli, as an N-terminal thioredoxin fusion protein, with the pET32a vector yielding a recombinant enzyme. Sequence analysis was used to compare this enzyme with the mechanistically related epi-aristolochene synthase from tobacco, and the effect of possible involvement of a number of amino acids in sesquiterpone synthase on product specificity was also discussed.

关键词： Germacrene A synthase Crepidiastrum sonchifolium Sesquiterpene synthase Expression Fusion protein

Algebraic immunities of vector-valued functions over finite fields

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The Journal of China Universities of Posts and Telecommunications 2015年第5期22卷 16-21页

作者： Liu Zhenhua Zhang jie wen Qiaoyan Computer science department China Women's University School of Science Beijing University of Posts and Telecommunications State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications

Algebraic immunity is an important cryptographic property of Boolean functions. The notion of algebraic immunity of Boolean functions has been generalized in several ways to vector-valued functions over arbitrary finite fields. In this paper, the results of Ref. [25] are generalized to arbitrary finite fields. We obtain vector-valued functions over arbitrary finite fields such that their algebraic immunities can reach the upper bounds. Furthermore, all the component functions, together with their some nonzero linear combinations, of vector-valued Boolean functions achieved by this construction have optimal algebraic immunities simultaneously.

关键词： cryptography Boolean functions algebraic immunity

Generalized Polynomial Chaos for Nonlinear Random Pantograph Equations

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Acta Mathematicae Applicatae Sinica 2016年第3期32卷 685-700页

作者： wen-jie SHI Cheng-jian ZHANG School of mathematics and statistics Huazhong University of Science and Technology School of Mathematics and Computer Science Wuhan Textile University

This paper is concerned with the application of generalized polynomial chaos （gPC） method to nonlinear random pantograph equations. An error estimation of gPC method is derived. The global error analysis is given for the error arising from finite-dimensional noise （FDN） assumption, projection error, aliasing error and discretization error. In the end, with several numerical experiments, the theoretical results are further illustrated.

关键词： generalized polynomial chaos random pantograph equations error estimation finite-dimensional noise

喹啉羧酸共价有机框架微球在线固相萃取4种黄曲霉毒素

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分析测试学报 2025年第1期44卷 163-171页

作者：王荣雨刘杰周静李涛王晓纪文华齐鲁工业大学(山东省科学院)山东省分析测试中心天然产物分离提取共性技术创新与应用山东省工程研究中心山东济南250014 山东明仁福瑞达制药股份有限公司山东济南250014 山东蓝城分析测试有限公司山东济南250100

以1,3,5-三(4-氨基苯基)苯、联苯二甲醛、丙酮酸为合成反应单体,氨基磺酸为催化剂,利用Doebner串联反应成功制备了具有喹啉羧酸骨架的共价有机框架(QCA-COF)微球。通过优化反应条件发现,采用1,4-二氧六环-乙腈溶液(1∶4,体积比)作为反... 详细信息

以1,3,5-三(4-氨基苯基)苯、联苯二甲醛、丙酮酸为合成反应单体,氨基磺酸为催化剂,利用Doebner串联反应成功制备了具有喹啉羧酸骨架的共价有机框架(QCA-COF)微球。通过优化反应条件发现,采用1,4-二氧六环-乙腈溶液(1∶4,体积比)作为反应溶剂时,可获得具有微米级粒径的微球。采用扫描电子显微镜、红外光谱、X射线粉末衍射、N_(2)吸脱附实验对QCA-COF微球的结构进行表征。由于QCA-COF微球具有芳环、喹啉基团、羧基等官能团,可通过氢键、π-π等多重相互作用与黄曲霉毒素进行吸附,其吸附能力优于C_(18)、PSA以及GCB等商用材料。将QCA-COF材料作为在线固相萃取柱的填料,详细优化了在线固相萃取条件。将在线固相萃取柱与高效液相色谱联用,建立了4种黄曲霉毒素的检测方法,检出限为0.024~0.030 ng·g^(-1)。经方法学验证,该方法具有较好的回收率(82.5%~105%)和精密度(4.3%~10%,n=6),适用于花生、小麦、陈皮、杏仁、决明子等食品与中药材中4种黄曲霉毒素的测定。

关键词：黄曲霉毒素在线固相萃取共价有机框架高效液相色谱

On Support τ-tilting Modules over Endomorphism Algebras of Rigid Ob jects

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Acta Mathematica Sinica,English Series 2015年第9期31卷 1508-1516页

作者： wen CHANG jie ZHANG Bin ZHU Department of Mathematical Sciences Tsinghua University School of Mathematics and Statistics Beijing institute of technology

We consider a Krull-Schmidt, Hom-finite, 2-Calabi Yau triangulated category with a basic rigid object T, and show a bijection between the set of isomorphism classes of basic rigid objects in the finite presented category pr T of T and the set of isomorphism classes of basic T-rigid pairs in the module category of the endomorphism algebra Endc（T）op. As a consequence, basic maximal objects in prT are one-to-one correspondence to basic support τ-tilting modules over Endc（T）op. This is a generalization of correspondences established by Adachi-Iyama-Reiten.

关键词： Rigid object maximal rigid object τ-rigid object finite presented category

On Ekeland's Variational Principle for Set-Valued Mappings

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Acta Mathematicae Applicatae Sinica 2007年第1期23卷 141-148页

作者： Sheng-jie Li wen-yan Zhang College of Mathematics and Science Chongqing University Chongqing 400044 China

In this paper, we derive a general vector Ekeland variational principle for set-valued mappings, which has a dosed relation to εk^0 -efficient points of set-valued optimization problems. The main result presented in ... 详细信息

关键词： Vector Ekeland variational principle nonlinear scalarization function metric space set-valued mapping

The Prolongation Structure of a Coupled Kdv Equation

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Acta Mathematicae Applicatae Sinica 2018年第2期34卷 430-437页

作者： Yang-jie JIA wen-shan DUAN College of Physics and Electronic Engineering Northwest Normal University Lanzhou 730070 China Department of Mathematics Nationalities College of Qinghai Normal University Xining 810008 China

The new coupled KdV equation proposed by Ohta and Hirota, in which the phase shift depends on the mutual positions of solitons at the initial time, is investigated in the framework of prolongation structure theory. It... 详细信息

关键词： integrable equation prolongation structure lax pair

Analysis of a Nutrient-phytoplankton Model in the Presence of Viral Infection

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Acta Mathematicae Applicatae Sinica 2016年第1期32卷 113-128页

作者： Jian-jun LI wen-jie GAO College of science Liaoning Technical University Institute of Mathematics Jilin University

In this paper,a system of reaction-diffusion equations arising in a nutrient-phytoplankton populations is *** equations model a situation in which phytoplankton population is divided into two groups,namely susceptible phytoplankton and infected phytoplankton.A number of existence and non-existence results about the non-constant steady states of a reaction diffusion system are *** the diffusion coefficient of the infected phytoplankton is treated as bifurcation parameter,non-constant positive steady-state solutions may bifurcate from the constant steady-state solution under some conditions.

关键词： coexistence bifurcations non-constant positive steady solution

A perspective on the electrocatalytic conversion of carbon dioxide to methanol with metallomacrocyclic catalysts

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Journal of Energy Chemistry 2022年第1期31卷 263-275,I0008页

作者： Xinyan Liu Bo-Quan Li Bing ni Lei Wang Hong-jie Peng Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 611731SichuanChina Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing 100081China Physical Chemistry Department of ChemistryUniversity of KonstanzUniversitatsstrasse 10D-78457 KonstanzGermany Department of Chemical and Biomolecular Engineering National University of Singapore.Singapore 117585Singapore

Electrocatalytic carb on dioxide reducti on(CO_(2)R)presents a promising route to establish zero-e mission carb on cycle and store in termittent ren ewable energy into chemical fuels for steady energy *** is an ideal energy carrier as alternative fuels and one of the most important commodity ***,methanol is currently mainly produced from fossil-based syngas,the production of which yields tremendous carb on emission *** CO_(2)R towards metha nol poses great potential to shift the paradigm of methanol *** this perspective,we focus our discussions on producing methanol from electrochemical CO_(2)R,using metallomacrocyclic molecules as the model *** discuss the motivation of having methanol as the sole CO_(2)R product,the documented application of metallomacrocyclic catalysts for CO_(2)R,and recent advance in catalyzing CO_(2) to methanol with cobalt phthalocyanine-based *** attempt to understand the key factors in determining the activity,selectivity,and stability of electrocatalytic CO_(2)-to-methanol conversion,and to draw mechanistic insights from existing ***,we identify the challenges hindering methanol electrosynthesis directly from CO_(2) and some intriguing directions worthy of further investigation and exploration.

关键词： Carbon dioxide reduction Methanol Electrocatalysis Molecular catalysts Single atom catalysts Heterogenization