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Science China Life Sciences 2025年第1期 300-302页

作者： Ze-Min Lin Mai Xiang wen-hui wei Shu-hui Fan Li Chen Jie Wang Xiao-Qian Yang Chun-Hao Yang Shi-Jun He Jian-Ping Zuo Laboratory of Immunopharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of Sciences School of Pharmacy University of Chinese Academy of Sciences School of Pharmacy Nanjing University of Chinese Medicine State Key Laboratory of Drug Research Department of Medicinal ChemistryShanghai Institute of Materia MedicaChinese Academy of Sciences Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine

Dear Editor, Phenotypic drug discovery, which observes compound effects on physiology,has given way to targeting specific molecules, but has revived by using modern methods to find drugs based on therapeutic outcomes in disease models(Vincent et al., 2022). Osteoarthritis(OA) is a prevalent chronic joint disorder, primarily affecting knee joints, with osteoclast activation due to low-grade inflammation resulting in increased bone resorption and structural changes in the joint(Katz et al.,2021).

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Karyotyping of Brassica napus L. Based on C0t-1 DNA Banding by Fluorescence In Situ Hybridization

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Journal of Integrative Plant Biology 2005年第12期47卷 1479-1484页

作者： wen-hui wei Wan-Peng ZHAO Li-Jun WANG Bo CHEN Yun-Chang LI Yun-Chun SONG Institute of Oil Crops Chinese Academy of Agricultural Sciences Wuhan 430062 China College of Life Sciences Xinyang Normal University Xinyang 464000 China Key Laboratory of Ministry of Education for Plant Developmental Biology College of Life Sciences Wuhan University Wuhan 430072 China

In order to precisely recognize and karyotype Brassica napus L. chromosomes, C0t-1 DNA was extracted from its genomic DNA, labeled with biotin-1 1-dUTP and in situ hybridized. The hybridized locations were detected by Cy3-conjugated streptavidin. Specific fluorescence in situ hybridization （FISH） signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both Cot-1 DNA FISH banding patterns and chromosome morphology.

关键词： Brassica napus C0t-1 DNA chromosome banding fluorescence in situ hybridization(FISH) karyotyping.

Cloning and Furictional Verification of Major Genes in Wheat Grain Number

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Cloning and Furictional Verification of Major Genes in Wheat...

Effects of NaCl stress on physiological and biochemical indexes and expression of related genes in wheat

第十届全国小麦基因组学及分子育种大会

作者： Liuping Chen Qiaoyan Chen wen-hui wei Life of Science and Technology Henan Institute of Science and Technology

Wheat is one of the world’s major food crops,with approximately35-40% of the world’s population being *** order to meet the demand for food in the future population increase,it is necessary to increase the output per unit *** the number of spikes per unit area reaches a certain range,the yield increase effect has reached a critical *** to the characteristics of the variety and environmental conditions,the increase in grain weight is *** number of kernels per spike is the most active factor in the yield component and can be adjusted to a greater ***,when wheat yield reaches a high level,increasing the number of wheat spikes becomes the main limiting factor for further increasing wheat *** this experiment,the F and low-generation backcross populations of Xianyang chaodasui and Baiqi Mai were *** target major QTL was identified by SSR marker combined with BSA method and wheat 660 K chip,and the molecular interval of the main QTL was *** near-isogenic line of the genetic locus uses the method of map-based cloning to clone the key genes controlling the number of kernels,and validates its biological function by OE and RNAi methods,and studies the expression of key genes in multi-peripheral number *** patterns and genetic interactions provide a theoretical basis for subsequent wheat new germplasm innovation and high-yield wheat breeding.

关键词： Spike grain number QTL SSR marker functional verification

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Effects of NaCl stress on physiological and biochemical inde...

Real-world evidence on the efficacy and safety of vonoprazanamoxicillin dual therapy for Helicobacter pylori treatment in elderly patients

第十届全国小麦基因组学及分子育种大会

作者： Liu-ping Chen Qing-feng Dong wen-hui wei Life of Science and Technology Henan Institute of Science and Technology

Wheat is the most productive crop in the *** production of wheat by salt is a threat to world food *** the development of molecular technology,it is very important to study the salt tolerance mechanism of wheat with the means of modern *** study uses five different wheat varieties as experimental material,probed into NaCl stress on its germination potential,germination rate,root length and shoot higher the change of the four index,according to the four traits index determination of NaCl stress on five salt resistance of different wheat *** filter out less resistance to salt and salt sensitive cultivar in seedling stage and filling stage,NaCl stress and measure its physiological indexes and photosynthetic character indexes of seedling stage and milking stage NaCl stress analysis the difference of salt resistance of different wheat *** this basis,the transcriptome sequencing was carried out using IlluminaHiSeq sequencing platform to find the related metabolic pathways of salt-tolerant genes,for the resistance of wheat salt regulation mechanism provides research foundation.

关键词： wheat NaCl stress expression of related genes

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World Journal of Gastroenterology 2025年第1期31卷 68-78页

作者： wen Gao Jing-wen Li hui Ye Xue-Zhi Zhang Jian-Xiang Liu Hong Cheng Department of Gastroenterology Peking University First HospitalBeijing 100034China School of Medicine Tsinghua MedicineTsinghua UniversityBeijing 100034China Department of Traditional Chinese Medicine and Integrative Medicine Peking University First HospitalBeijing 100034China

BACKGROUND A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of Helicobacter pylori(***).While substantial research supports the efficacy and safety of vonoprazan and amoxicillin(VA)dual therapy in the general population,there is still a lack of studies specifically focusing on its safety in elderly *** To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for *** in elderly *** As a real-world retrospective study,data were collected from elderly patients aged 60 years and above who accepted VA dual therapy(vonoprazan 20 mg twice daily+amoxicillin 1000 mg thrice daily for 14 days)for *** eradication in the Department of Gastroenterology at Peking University First Hospital between June 2020 and January *** status was evaluated by^(13)C-urease breath test 6 weeks after *** adverse events(AEs)during treatment were *** In total,401 cases were ***-one cases were excluded due to loss to follow-up,lack of re-examination,or unwillingness to take *** total of 380 included cases comprised 250 who received VA dual therapy as first-line treatment and 130 who received VA dual therapy as rescue *** was successfully eradicated in 239 cases(95.6%)in the first-line treatment group and 116 cases(89.2%)in the rescue treatment *** overall incidence of AEs was 9.5%for both ***,9.2%of patients experienced an AE in the first-line treatment group and 10.0%in the rescue treatment *** patients discontinued treatment due to AE,with a discontinuation rate of 1.3%.No serious AE *** The VA dual therapy regimen as a first-line treatment and a rescue therapy was effective and safe for elderly patients aged 60 and older.

关键词： Helicobacter pylori Vonoprazan Amoxicillin Dual therapy Elderly

Loss-of-function mutations of microRNA-142-3p promote ASH1L expression to induce immune evasion and hepatocellular carcinoma progression

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World Journal of Gastroenterology 2025年第1期31卷 126-145页

作者： Xing-hui Yu Yan Xie Jian Yu Kun-Ning Zhang Zhou-Bo Guo Di Wang Zhao-Xian Li wei-Qi Zhang Yu-Ying Tan Li Zhang wen-Tao Jiang School of Medicine Nankai UniversityTianjin 300192China Tianjin Key Laboratory of Molecular Diagnosis and Treatment of Liver Cancer Tianjin First Center HospitalTianjin 300192China Department of Liver Transplantation Tianjin First Center HospitalTianjin 300192China First Central Clinical School Tianjin Medical UniversityTianjin 300192China

BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated *** therapeutic targets are beneficial to treat and predict the ***,the exact molecular mechanisms leading to the progression of HCC are still *** has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor *** this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between *** To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC *** In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC ***,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,*** statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank *** In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival ***1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated ***,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via *** the exploration mechanism,we found ASH1L may promote an immunosuppressive mi

关键词： Hepatocellular carcinoma MicroRNA-142-3p ASH1L Immune evasion Tumor immune microenvironment Apoptosis

基于Riccati方程的稳态传感器分配算法研究

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系统工程与电子技术 2004年第7期26卷 863-866,886页

作者：周文辉胡卫东郁文贤国防科技大学电子科学与工程学院ATR重点实验室湖南长沙410073

采用协方差控制策略 ,建立了传感器分配的最优化模型 ,提出一种基于Riccati方程的稳态传感器分配算法。在预先给定稳态期望协方差的情形下 ,该算法通过求解Riccati方程计算出各种传感器组合的效能 ,并选择使目标函数值最小的传感器组合... 详细信息

采用协方差控制策略 ,建立了传感器分配的最优化模型 ,提出一种基于Riccati方程的稳态传感器分配算法。在预先给定稳态期望协方差的情形下 ,该算法通过求解Riccati方程计算出各种传感器组合的效能 ,并选择使目标函数值最小的传感器组合进行分配。该算法可以独立于跟踪循环之外预先计算好传感器分配方案 ,因而是一种离线的分配方法。给出了应用该方法的具体步骤。

关键词：传感器分配传感器管理 Riccati方程协方差控制多传感器系统

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Effects of atorvastatin on progression of diabetic nephropathy and local RAGE and soluble RAGE expressions in rats

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第8期 651-651页

作者： Lin Lu, wen-hui Peng, wei Wang, Ling-jie Wang, Qiu-jing Chen, wei-feng Shen

Objective:Advanced glycation end-products (AGEs) exert inflammatory and oxidative stress insults to produce diabetic nephropathy mainly through the receptor for AGEs (RAGE). This study aimed to assess the effect of atorvastatin on diabetic nephropathy via soluble RAGE (sRAGE) and RAGE expressions in the rat kidney. Methods:Thirty-two male Sprague-Dawley rats were divided into four groups based on the presence or absence of streptozotocin-induced diabetes with or without atorvastatin treatment (10 mg/kg for 24 weeks). Serum sRAGE and glycated albumin (GA) levels were measured with enzyme-linked immunosorbent assay (ELISA) and improved bromocresol purple methods. Renal AGEs, RAGE, endogenous secretory RAGE (es-RAGE), and sRAGE were determined with reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Results:Mesangial expansion and microalbuminuria were aggravated in diabetic rats, and improved with atorvastatin treatment. Serum sRAGE levels were lower in diabetic than in normal rats. After atorvastatin treatment, serum and renal sRAGE levels were up-regulated, while renal RAGE expression was decreased in diabetic rats, associated with a reduction in accumulation of AGEs, though renal esRAGE mRNA expression was not significantly increased. Conclusions:Atorvastatin exerted a beneficial effect on diabetic nephropathy with reduced AGE accumulation, down-regulating RAGE expression and up-regulating sRAGE in the kidney.

关键词： RAGE Effects of atorvastatin on progression of diabetic nephropathy and local RAGE and soluble RAGE expressions in rats AGEs

TRRE发动机关键技术分析及推进性能探索研究

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推进技术 2017年第2期38卷 298-305页

作者：韦宝禧凌文辉冮强魏祥庚秦飞何国强北京动力机械研究所高超声速冲压发动机技术重点实验室北京100074 西北工业大学燃烧热结构与内流场重点实验室陕西西安710072

对TRRE发动机关键技术难点及技术途径进行了初步分析,对北京动力机械研究所原理样机研制进展进行了简要介绍,并结合原理样机流道设计与数值仿真研究获得的性能数据,开展了TRRE发动机推进性能探索研究,初步验证了TRRE发动机在兼具加速、... 详细信息

对TRRE发动机关键技术难点及技术途径进行了初步分析,对北京动力机械研究所原理样机研制进展进行了简要介绍,并结合原理样机流道设计与数值仿真研究获得的性能数据,开展了TRRE发动机推进性能探索研究,初步验证了TRRE发动机在兼具加速、巡航、机动等方面的性能优势。研究结果表明,在适当兼顾低马赫数大推力需求和Ma=6纯冲压工况高比冲需求的情况下,发动机在宽马赫数工作范围具有较大范围、灵活便捷的推力调节能力,可满足高低速通道平稳接力需求,表现出极具优势的加速、机动飞行和高马赫数巡航的适应性。

关键词：临近空间及空天飞行器组合发动机性能分析

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基于NNI注视指数的塔台管制员情境意识研究

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科学技术与工程 2016年第31期16卷 101-105页

作者：靳慧斌刘亚威刘文辉张程嵬中国民航大学飞行技术学院天津300300

为研究塔台管制员在任务不同阶段的注视模式与情境意识(situation Awareness,SA)的关系,搭建模拟塔台管制的眼动试验平台,设计3组不同难度的试验,用NASA-TLX量表评估被试的任务负荷,利用方差分析方法验证试验难度设计的合理性;利用3D-S... 详细信息

为研究塔台管制员在任务不同阶段的注视模式与情境意识(situation Awareness,SA)的关系,搭建模拟塔台管制的眼动试验平台,设计3组不同难度的试验,用NASA-TLX量表评估被试的任务负荷,利用方差分析方法验证试验难度设计的合理性;利用3D-SART量表评估被试的SA水平,利用NNI算法分别计算和识别试验前期和后期被试的注视模式,并结合操作绩效,分析不同任务难度下管制员前期和后期的NNI注视指数与SA的关系。结果表明,被试在试验后期的NNI注视指数受试验难度的影响更为显著,且试验后期NNI注视模式呈离散型分布的被试具有更高的SA水平和操作绩效。

关键词：管制员情境意识(SA) 最近邻指数(NNI) 注视模式

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