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Stability and transepithelial transport of oligopeptide(KRQKYD)with hepatocyte-protective activity from Jinhua ham in human intestinal Caco-2 monolayer cells

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Food Science and Human Wellness 2024年第3期13卷 1503-1512页

作者： wen Nie Feiran xu Kai Zhou Jieying Deng Ying Wang Baocai xu Engineering Research Center of Bio-process Ministry of EducationSchool of Food and Biological EngineeringHefei University of TechnologyHefei 230601China School of Biological and Food Engineering Chuzhou UniversityChuzhou 239001China

The study evaluated the stability of an oligopeptide(Lys-Arg-Gln-Lys-Tyr-Asp,KRQKYD)and its transport mechanism by simulating gastrointestinal digestion and a model of human intestinal Caco-2 monolayer cells in *** this study,the effects of environmental factors(temperature,pH and NaCl concentration)and simulated gastrointestinal digestion on the stability of KRQKYD were evaluated by indicators of the levels of alanine transaminase(ALT),aspartate transaminase(AST)and malondialdehyde(MDA)in an alcoholinduced hepatocyte injury *** results showed that KRQKYD still maintained satisfactory hepatocyteprotective activity after treatment with different temperatures(20-80℃),pH(3.0-9.0),NaCl concentration(1%-7%)and simulated gastrointestinal digestion,which indicated that KRQKYD showed good stability to environmental factors and simulated gastrointestinal ***,the intact KRQKYD could be absorbed in a model of Caco-2 monolayer cells with a P_(app)value of(9.70±0.53)×10^(-7)cm/*** with an energy inhibitor(sodium azide),a competitive peptide transporter inhibitor(Gly-Pro)and a transcytosis inhibitor wortmannin did not decrease the level of transepithelial KRQKYD transport,indicating that the transport mechanism of KRQKYD was not associated with energy dependent,vector mediated and *** tight junction disruptor cytochalasin D significantly increased the level of transepithelial KRQKYD transport(P<0.05),suggesting that intact KRQKYD was absorbed by paracellular transport.

关键词： Jinhua ham KRQKYD(Lys-Arg-Gln-Lys-Tyr-Asp) Environmental stability Gastrointestinal digestive Transport mechanism

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Accurate characterization of bubble mixing uniformity in a circular region using computational geometric theory

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Particuology 2024年第3期86卷 149-159页

作者： Hui Sun Jianxin xu Shibo Wang Guangcheng cai Hua Wang Wuliang Yin State Key Laboratory ofComplex Nonferrous Metal Resources Clean Utilization Kunming University ofScience and TechnologyKunming650093China bFaulty of Metallurgical and Energy Engineering Kunming University ofScience and TechnologyKunming650093China Faculty of Science Kunming University ofScience and TechnologyKunming650500China Department of Elec.and Electronic Engineering.University of Manchester ManchesterM139PL UK

The present study proposes a novel method based on the geometric theory for measuring the distribution of bubble swarms in the circular region of a direct-contact heat *** was determined that the mixing is uniform when the average distance between the bubble swarms in the unit circular region is approximately 0.9054,which is the standard reference *** effect of sample size(i.e.,the number of bubbles)on mixing uniformity was investigated to determine the optimal sample *** was verified that the metric's accuracy and stability were higher with a sample size of ***,it was proposed to increase the sample size by filling irregular bubbles using a segmentation method,which enabled a further accurate assessment of the mixing *** mixing uniformity of bubble swarms in the circular region and its maximum internal connection with the square region was accurately *** was revealed that the relative average error increased by approximately 3.47% due to information *** proposed method was demonstrated to be rotationally *** present study provided novel insights into evaluating mixing uniformity,which would guide enhanced heat transfer and the effective evaluation of the spatiotemporal characteristics of transient mixing in circular regions or the cross-sections of chemical transport pipelines.

关键词： Direct-contact heat exchanger Circular region Bubble swarm Distribution uniformity AD-Circle method

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Analytical libration control law for electrodynamic tether system with current constraint

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Astrodynamics 2024年第2期8卷 237-246页

作者： Shidong xu Ti Chen Hao wen Dongping Jin State Key Laboratory of Mechanics and Control for Aerospace Structures Nanjing University of Aeronautics and AstronauticsNanjing210016China

This study focuses on stabilizing the libration dynamics of an electrodynamic tether system(EDTS)using generalized torques induced by the Lorentz *** contrast to existing numerical optimization methods,a novel analytical feedback control law is developed to stabilize the in-plane and out-of-plane motions of a tether by modulating the electric current *** saturation constraint on the current is accounted for by adding an auxiliary dynamic system to the *** enhance the robustness of the proposed controller,multiple perturbations of the orbital dynamics,modeling uncertainties,and external disturbances are approximated using a neural network in which the weighting matrix and approximation error are estimated simultaneously,such that these perturbations are well compensated for during the control design of the ***,a dynamically scaled generalized inverse is utilized to address the singular matrix in the control *** closed-loop system is proven to be ultimately bounded based on Lyapunov stability ***,numerical simulations are performed to demonstrate the effectiveness of the proposed analytical control law.

关键词： electrodynamic tether system(EDTS) libration dynamics analytical control current constraint

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Non-Gaussian feature of the output field from a double qubit-cavity ultrastrong coupling system

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Communications in Theoretical Physics 2024年第7期76卷 30-36页

作者： Sheng-wen xu Shi-yu Dai Teng Zhao Center for Quantum Science and Technology Jiangxi Normal UniversityNanchang 330022China

We investigate the non-Gaussian feature of radiation in a circuit quantum electrodynamics(QED)system where two qubits are strongly coupled to a single-mode *** the regime of ultrastrong coupling(USC),the rotating-wave approximation is not valid,and the Rabi Hamiltonian contains counter-rotating wave terms,leading to level crossing and avoided crossings in the energy *** further analyze the intensity-amplitude correlation of the output field in these two novel *** the USC regime,the creation and annihilation operators in the correlation function are replaced,allowing for the identification of non-Gaussian features in the output *** findings reveal that despite the absence of squeezing effects in the output light,significant non-Gaussian characteristics are ***,we demonstrate that as the driving or coupling strength increases,the non-Gaussian features of the output field become more *** suggests that USC systems hold broad potential applications in the realms of nonlinear optics and the generation of non-Gaussian states.

关键词： ultrastrong coupling non-Gaussian intensity-amplitude correlation

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DeepCRBP:improved predicting function of circRNA-RBP binding sites with deep feature learning

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Frontiers of Computer Science 2024年第2期18卷 235-237页

作者： Zishan xu Linlin SONG Shichao LIU wen ZHANG College of Informatics Huazhong Agricultural UniversityWuhan 430070China

1 Introduction Recently,there is growing evidence that it is essential to predict the interactions between circRNAs and RBP binding sites for diagnosing diseases and providing a potential target to treat diseases[1].Many studies have predicted the binding sites of circRNA-RBPs by using deep learning methods based on the sequence information of circRNAs for each ***,many methods focus on diversified view of sequence encoding for improving the performance of the predictive models.

关键词： learning Deep function

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Large-Scale Multi-Objective Optimization Algorithm Based on Weighted Overlapping Grouping of Decision Variables

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Computer Modeling in Engineering & Sciences 2024年第7期140卷 363-383页

作者： Liang Chen Jingbo Zhang Linjie Wu Xingjuan cai Yubin xu Shanxi Key Laboratory of Big Data Analysis and Parallel Computing Taiyuan University of Science and TechnologyTaiyuan030024China School of State Key Laboratory of Novel Software Technology Nanjing UniversityNanjing210008China

The large-scale multi-objective optimization algorithm(LSMOA),based on the grouping of decision variables,is an advanced method for handling high-dimensional decision ***,in practical problems,the interaction among decision variables is intricate,leading to large group sizes and suboptimal optimization effects;hence a large-scale multi-objective optimization algorithm based on weighted overlapping grouping of decision variables(MOEAWOD)is proposed in this ***,the decision variables are perturbed and categorized into convergence and diversity variables;subsequently,the convergence variables are subdivided into groups based on the interactions among different decision *** the size of a group surpasses the set threshold,that group undergoes a process of weighting and overlapping ***,the interaction strength is evaluated based on the interaction frequency and number of objectives among various decision *** decision variable with the highest interaction in the group is identified and disregarded,and the remaining variables are then reclassified into ***,the decision variable with the strongest interaction is added to each *** minimizes the interactivity between different groups and maximizes the interactivity of decision variables within groups,which contributed to the optimized direction of convergence and diversity exploration with different *** was subjected to testing on 18 benchmark large-scale optimization problems,and the experimental results demonstrate the effectiveness of our *** with the other algorithms,our method is still at an advantage.

关键词： Decision variable grouping large-scale multi-objective optimization algorithms weighted overlapping grouping direction-guided evolution

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Study on metal recovery process and kinetics of oxidative leaching from spent LiFePO_(4)Li-batteries

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Chinese Journal of Chemical Engineering 2024年第4期68卷 94-102页

作者： Xiaoming Zhang wen Xie Xiaolei Zhou wenjie Zhang Jiawei wen Xin Wang Guoyong Huang Shengming xu College of New Energy and Materials China University of PetroleumBeijing 102249China State Key Laboratory of Heavy Oil Processing College of Chemical EngineeringChina University of PetroleumBeijing 102249China Beijing Spacecrafts Beijing 100094China Ganfeng Lithium Group Co. Ltd.Xinyu 338000China Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing 100084China Beijing Key Laboratory of Fine Ceramics Tsinghua UniversityBeijing 100084China

A green environmental protection and enhanced leaching process was proposed to recover all elements from spent lithium iron phosphate(LiFePO_(4)) lithium *** order to reduce the influence of Al impurity in the recovery process,NaOH was used to remove *** impurity removal,the spent LiFePO_(4) cathode material was used as raw material under the H_(2)SO_(4) system,and the pressure oxidation leaching process was adopted to achieve the preferential leaching of *** E-pH diagram of the Fe-P-Al-H_(2)O system can determine the stable region of each element in the recovery process of spent LiFePO_(4)*** the optimal conditions(500 r·min^(-1),15 h,363.15 K,0.4 MPa,the liquid-solid ratio was 4:1 ml·g^(-1)and the acid-material ratio was 0.29),the leaching rate of Li was 99.24%,Fe,Al,and Ti were 0.10%,2.07%,and 0.03%,*** Fe and P were precipitated and recovered as FePO_(4)·2H_(2)*** kinetic analysis shows that the process of high-pressure acid leaching of spent LiFePO_(4) materials depends on the surface chemical *** the life cycle assessment(LCA)of the spent LiFePO_(4) whole recovery process,eight midpoint impact categories were selected to assess the impact of recovery *** results can provide basic environmental information on production process for recycling industry.

关键词： Spent LiFePO_(4)Li-batteries Oxidative leaching Kinetic study Life cycle assessment

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Performance-control-orientated hybrid metal additive manufacturing technologies:state of the art,challenges,and future trends

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International Journal of Extreme Manufacturing 2024年第3期6卷 274-328页

作者： Jiming Lv Yuchen Liang Xiang xu Gang xu Hongmei Zhang Haifei Lu Kaiyu Luo Jie cai Jinzhong Lu School of Mechanical Engineering Jiangsu UniversityZhenjiang 212013People’s Republic of China School of Mechanical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003People’s Republic of China

Metal additive manufacturing(AM)technologies have made significant progress in the basic theoretical field since their invention in the ***,performance instability during continuous processing,such as thermal history,residual stress accumulation,and columnar grain epitaxial growth,consistently hinders their broad application in standardized industrial *** overcome these challenges,performance-control-oriented hybrid AM(HAM)technologies have been *** technologies,by leveraging external auxiliary processes,aim to regulate microstructural evolution and mechanical properties during metal *** paper provides a systematic and detailed review of performance-control-oriented HAM technology,which is categorized into two main groups:energy field-assisted AM(EFed AM,***,electromagnetic,and heat)technologies and interlayer plastic deformation-assisted AM(IPDed AM,*** shock peening,rolling,ultrasonic peening,and friction stir process)*** review covers the influence of external energy fields on the melting,flow,and solidification behavior of materials,and the regulatory effects of interlayer plastic deformation on grain refinement,nucleation,and ***,the role of performance-control-oriented HAM technologies in managing residual stress conversion,metallurgical defect closure,mechanical property improvement,and anisotropy regulation is thoroughly reviewed and *** review concludes with an analysis of future development trends in EFed AM and IPDed AM technologies.

关键词： hybrid additive manufacturing in-situ/interlayer plastic deformation auxiliary energy fields microstructure customization mechanical properties enhancement

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Graphitic carbon nitride nanosheets mitigate cadmium toxicity in Glycine max *** promoting cadmium retention in root and improving photosynthetic performance

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Journal of Environmental Sciences 2024年第5期139卷 543-555页

作者： Kai xu Yunyu Guo Chenghua Xing Ronglong Fu Bin Zou Rongchuan Liu Luyi cai Jianfang Yan Xi-Lin Wu Miaozhen cai College of Geography and Environmental Sciences Zhejiang Normal UniversityJinhua 321004China College of Agriculture Jinhua PolytechnicJinhua 321007China

Cadmium(Cd)pollution poses a serious threat to plant growth and *** have shown great application potential for alleviation of Cd toxicity to *** this study,we applied graphitic carbon nitride nanosheets(g-C_(3)N_(4)NSs)for alleviation of Cd-toxicity to soybean(Glycine max L.).The g-C_(3)N_(4)NSs supplementation significantly improved plant growth and reduced oxidative damage in the Cd-toxicated soybean seedlings through hydroponic ***,the g-C_(3)N_(4)NSs dynamically regulated the root cell wall(RCW)components by increasing pectin content and modifying its demethylation via enhancing pectin methylesterase(PME)activity,therefore greatly enhanced stronger RCW-Cd retention(up to 82.8%)and reduced Cd migration to the ***,the g-C_(3)N_(4)NSs reversed the Cd-induced chlorosis,increased photosynthetic efficiency because of enhancement in F_v/F_mration,Y(Ⅱ)and sugars *** results provide new insights into the alleviation of Cd toxicity to plants by g-C_(3)N_(4)NSs,and shed light on the application of low-cost and environmental-friendly carbon-based NMs for alleviating heavy metal toxicity to plants.

关键词： Cadmium Carbon nitride nanosheets Cell wall Pectin Pectin methylesterase Soybean

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Intracellular redox potential-driven live-cell synthesis of CdSe quantum dots in Staphylococcus aureus

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Science China Chemistry 2024年第3期67卷 990-999页

作者： cai-Qin Wu An-An Liu xu Li Jia-Wei Tu Juan Kong Ling-Ling Yang Jian-Hong Jia Chuan Wang Bin Hu Zhi-Xiong Xie Dai-wen Pang The Institute for Advanced Studies College of Chemistry and Molecular SciencesCollege of Life SciencesHubei Key Laboratory of Cell HomeostasisWuhan UniversityWuhan 430072China Research Center for Analytical Sciences College of ChemistryState Key Laboratory of Medicinal Chemical BiologyTianjin Key Laboratory of Biosensing and Molecular RecognitionFrontiers Science Center for New Organic MatterFrontiers Science Center for Cell Responsesand Haihe Laboratory of Sustainable Chemical TransformationsNankai UniversityTianjin 300071China

Biosynthesized semiconductor quantum dots(QDs) have bright fluorescence,adjustable particle sizes,and environmental friendliness,endowing them with convenience and potential for biological *** to the unclear mechanism of the cellular environment on the live-cell synthesis of QDs,it is still difficult to regulate their optical ***,the critical role of the intracellular redox environment in regulating the fluorescence properties of biosynthesized CdSe QDs in Staphylococcus aureus(***) has been *** glutathione peroxidase(GPx) activity directly affects the intracellular H_(2)O_(2) produced in the SeO_(3)^(2-) reduction,which further manipulates the glutathione redox cycle to determine the content of low-valence *** a result,the fluorescence intensity of the synthesized CdSe QDs increases by 60% in the GPx overexpressed *** cellular redox potential that is controlled by the GSH redox cycle provides the driving force for the reduction of SeO_(3)^(2-),facilitating the synthesis of CdSe QDs in *** *** proposed mechanism of the cellular redox state provides a new perspective for regulating the synthesis of semiconductor nanomaterials in live cells.

关键词： redox H_(2)O_(2) peroxidase quantum dot Staphylococcus aureus

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