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Melatonin potentiates glycine currents through distinct PC-PLC/PKC signaling pathway in rat retinal ganglion cells

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Melatonin potentiates glycine currents through distinct PC-P...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： wen-Jie zhao,Min zhang,Xiong-Li YANG*,Zhong-Feng WANG~* Institute of Neurobiology,Institutes of Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032,China

In vertebrate retina,melatonin regulates various physiological *** previous work demonstrated potentiation of glycine currents by melatonin in rat retinal ganglion cells(RGCs) via activating melatonin MT2 receptors. In the present work we investigated the mechanisms underlying the melatonin effects by whole-cell patch-clamp technique. Immunofluorescence double labeling showed that rat RGCs were solely immunoreactive to MT2 *** potentiated glycine currents of rat RGCs and promoted their desensitization,which were reversed by 4-P-PDOT,a specific antagonist of MT2 *** effects of melatonin were blocked by intracellular dialysis of GDP-β-S,a nonhydrolyzable inhibitor of G-proteins,and antibodies against regulator of G-protein signaling(RGS) 2 and 4 *** addition, preincubation with pertussis toxin(PTX) eliminated the melatonin-induced potentiation of glycine *** these results suggest that the melatonin effects were mediated by PTX-sensitive Gi/o ***,D609,a phosphatidylcholine (PC)-specific phospholipase C(PLC) inhibitor,but not U73122,a phosphatidylinositol(PI)-PLC inhibitor, blocked the melatonin *** protein kinase C(PKC) activator PMA potentiated the glycine currents,thus mimicking the effects of melatonin,and the presence of PMA melatonin no longer caused further potentiation of the currents, whereas application of the PKC inhibitor bisindolylmaleimideⅣ(BisⅣ) abolished the melatonin *** melatonininduced potentiation of glycine currents was calcium-independent,as evidenced by the fact that the effects persisted when intracellular Ca was chelated by *** shown by calcium imaging,melatonin induced no increase in intracellular *** cAMP-PKA nor cGMP-PKG signaling pathways seemed to be involved because application of 8-BrcAMP or 8-Br-cGMP failed to mimic the melatonin *** both the PKA inhibitor H-89 and the PKG inhibitor KT5823 did not block the melatonin-induced potent

关键词： melatonin MT2 receptor glycine receptor Gi/o PC-PLC/PKC pathway RGS2/4 ganglion cell retina

来源： cnki会议评论

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基于TENA的虚拟试验实现技术研究

系统仿真学报 2011年第5期23卷 857-863页

作者：代坤赵雯张灏龙施国强张耀磊中国运载火箭技术研究院研究发展中心北京100076

虚拟试验支撑框架研究是引领整个虚拟试验验证系统快速有效发展的核心,也是试验测试领域的研究重点之一。针对虚拟试验验证技术的应用背景,研究了美国国防部开发的试验与训练使能结构(TENA),详细分析了体系结构框架及若干关键技术,重点... 详细信息

虚拟试验支撑框架研究是引领整个虚拟试验验证系统快速有效发展的核心,也是试验测试领域的研究重点之一。针对虚拟试验验证技术的应用背景,研究了美国国防部开发的试验与训练使能结构(TENA),详细分析了体系结构框架及若干关键技术,重点包括TENA元模型,TENA中间件,TENA与HLA的关系。最后提出了采用TENA技术的虚拟试验验证技术的研究思路,并对虚拟试验验证使能支撑框架(VITA)中涉及到的共性技术和应用层面的关键技术进行了简要阐述。

关键词： TENA 虚拟试验元模型中间件

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直线导轨式隔震装置的设计与性能研究

地震工程学报 2025年第1期47卷 50-59页

作者：赵守江柏文故宫博物院北京100009 中国地震局工程力学研究所地震工程与工程振动重点实验室黑龙江哈尔滨150080 地震灾害防治应急管理部重点实验室黑龙江哈尔滨150080

为确保馆藏文物的地震安全,研制出一款新型直线导轨式隔震装置,采用振动台试验和理论分析方法对其有效性进行验证。首先阐述装置的构造和工作机理,推导出装置的刚度和黏滞阻尼系数表达式;然后制作原型样机并通过振动台试验验证其隔震有... 详细信息

为确保馆藏文物的地震安全,研制出一款新型直线导轨式隔震装置,采用振动台试验和理论分析方法对其有效性进行验证。首先阐述装置的构造和工作机理,推导出装置的刚度和黏滞阻尼系数表达式;然后制作原型样机并通过振动台试验验证其隔震有效性;最后依据所测基本参数,建立装置单向水平隔震系统的动力方程并开展数值分析。结果表明,该隔震装置结构简单、性能可靠,具有摩擦系数低、起滑加速度阈值小,以及刚度和阻尼灵活可调的特点;它可以在满足位移限值的情况下达到设定隔震目标,且自复位效能力强、残余位移小。装置理论分析结果与试验实测动力响应吻合程度高,且吻合程度随地震动峰值的提高而进一步增强。该理论分析方法可以用于指导装置参数设计和隔震性能评估。

关键词：文物隔震直线导轨式振动台试验数值模拟

Modeling and control of a rigid-flexible coupling robot for narrow space manipulations

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Science China Technological Sciences 2025年

作者： Kuangda CHEN Huan zhao Jiexin zhang Mei FENG Yiwei WANG Jinliang WANG Han DING State Key Laboratory of Intelligent Manufacturing Equipment and Technology Huazhong University of Science and Technology Key Laboratory of CNC Equipment Reliability Ministry of Education Jilin University School of Medical Equipment Science and Engineering Huazhong University of Science and Technology

Narrow space manipulations are crucial for equipment maintenance, as they eliminate the need for disassembly, thus reducing costs and increasing efficiency. Cable-driven flexible robots possess exceptional capabilities in maneuvering through narrow spaces, thanks to their interaction compliance and distal dexterity. However, their inherent low stiffness causes a decline in the location accuracy of the robot and could even lead to manipulation failure. To address this challenge, this paper proposes a novel type of rigid-flexible coupling robot, which increases the stiffness of the robot while ensuring the reachability within a narrow space. To achieve high-accuracy location in a narrow space, kinematic and control methods are proposed. Specifically,the robot forward kinematics are uniformly represented, and the closed-form inverse kinematics solution is derived. Furthermore, in cases where the robot?s structure fails to align with Pieper?s solution, a two-stage search strategy is utilized to solve the inverse kinematics, resulting in a high success rate of 99.1%. Based on the proposed kinematics, a rigid-dominated control method is then proposed to facilitate localization and pose alignment of target objects in narrow spaces. The practical comparative experiments show that the proposed robot has the capability of high accuracy control in narrow spaces.

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增程式电动客车光纤CAN总线及调制解调器设计

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电源技术 2014年第11期38卷 2169-2170页

作者：陈洪图张洪永贾金林赵文章张一松赛恩斯能源科技有限公司浙江杭州310030 天津百特海洋工程设计有限公司天津300100

以超级电容与锂动力电池的增程式客车为原型车,研究基于光纤的整车CAN网络的设计与应用,解决整车节点之间通讯的串扰问题,并对其性能进行测试;设计光纤CAN调制解调器,以便不具备光纤通信功能的节点与其他节点实时交互,使得通讯更为安全... 详细信息

以超级电容与锂动力电池的增程式客车为原型车,研究基于光纤的整车CAN网络的设计与应用,解决整车节点之间通讯的串扰问题,并对其性能进行测试;设计光纤CAN调制解调器,以便不具备光纤通信功能的节点与其他节点实时交互,使得通讯更为安全、通畅和高效。

关键词：增程式客车光纤CAN网络光纤调制解调器

Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： Jing Wang Bing zhang Lanfang Li Xiaomei Tang Jinyu Zeng Yige Song Chao Xu Kai zhao Guoqiang Liu Youming Lu Xinyan Li Kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury

Nanocarrier-mediated siRNA delivery:a new approach for the treatment of traumatic brain injury-related Alzheimer's disease

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Neural Regeneration Research 2025年第9期20卷 2538-2555页

作者： Jie Jin Huajing zhang Qianying Lu Linqiang Tian Sanqiao Yao Feng Lai Yangfan Liang Chuanchuan Liu Yujia Lu Sijia Tian Yanmei zhao wenjie Ren Institute of Disaster and Emergency Medicine Tianjin UniversityTianjinChina Key Laboratory for Disaster Medicine Technology TianjinChina Henan Medical Key Laboratory for Research of Trauma and Orthopedics The Third Affiliated Hospital of Xinxiang Medical UniversityXinxiangHenan ProvinceChina Clinical Medical Center of Tissue Engineering and Regeneration Xinxiang Medical UniversityXinxiangHenan ProvinceChina Institutes of Health Central Plain Xinxiang Medical UniversityXinxiangHenan ProvinceChina School of Public Health Xinxiang Medical UniversityXinxiangHenan ProvinceChina

Traumatic brain injury and Alzheimer's disease share pathological similarities,including neuronal loss,amyloid-βdeposition,tau hyperphosphorylation,blood-brain barrier dysfunction,neuroinflammation,and cognitive ***,traumatic brain injury can exacerbate Alzheimer's disease-like pathologies,potentially leading to the development of Alzheimer's *** offer a potential solution by facilitating the delive ry of small interfering RNAs across the blood-brain barrier for the targeted silencing of key pathological genes implicated in traumatic brain injury and Alzheimer's disease.U nlike traditional approaches to neuro regeneration,this is a molecula r-targeted strategy,thus avoiding non-specific drug *** review focuses on the use of nanocarrier systems for the efficient and precise delive ry of siRNAs,discussing the advantages,challenges,and future *** principle,siRNAs have the potential to target all genes and non-targetable protein s,holding significant promise for treating various *** the various therapeutic approaches currently available for neurological diseases,siRNA gene silencing can precisely"turn off"the expression of any gene at the genetic level,thus radically inhibiting disease progression;however,a significant challenge lies in delivering siRNAs across the blood-brain *** have received increasing attention as an innovative drug delive ry tool fo r the treatment of brain *** are considered a potential therapeutic strategy with the advantages of being able to cross the blood-brain barrier,targeted drug delivery,enhanced drug stability,and multifunctional *** use of nanoparticles to deliver specific modified siRNAs to the injured brain is gradually being recognized as a feasible and effective *** this strategy is still in the preclinical exploration stage,it is expected to achieve clinical translation in the future,creating a new field of molecular tar

关键词： blood-brain barrier cognitive dysfunction exosomes gene silencing immunotherapy inorganic nanomaterials liposome nanoparticles neuroinflammation polymer nanoparticles stem cells

加速康复外科在结直肠癌手术中的效果评价及影响因素分析

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肠外与肠内营养 2018年第4期25卷 228-233页

作者：高向东黄彬沈烨卢健闻兆章南京大学医学院江苏南京210002 张家港澳洋医院江苏张家港215600

目的:评估结直肠手术应用加速康复外科(ERAS)的临床效果,分析影响ERAS实施效果的相关因素。方法:回顾性分析了张家港澳洋医院2015年1月到2017年2月收治的167例因结直肠癌行结直肠切除的病人的临床资料。其中,2015年1月到2016年3月,共89... 详细信息

目的:评估结直肠手术应用加速康复外科(ERAS)的临床效果,分析影响ERAS实施效果的相关因素。方法:回顾性分析了张家港澳洋医院2015年1月到2017年2月收治的167例因结直肠癌行结直肠切除的病人的临床资料。其中,2015年1月到2016年3月,共89例病人采用传统的围手术期处理方案,设为传统方案组。从2016年4月到2017年2月,共78例病人采用ERAS围手术期处理方案,设为ERAS组。对两组病人基线资料,围手术期情况以及术后结局进行比较分析。结果:ERAS组和传统方案组相比,两组的基线数据如年龄、性别、BMI、肿瘤部位、肿瘤组织学分级及分期之间没有显著性差异(P>0.05)。术中情况比较,ERAS组更多的采用腹腔镜的手术方式,术中需要输血的病人比例低于传统方案组(P<0.05)。术后结局比较,ERAS组在术后排气时间、术后住院时间以及术后ICU住院时间均低于传统方案组,且术后I级并发症以及II级并发症的发生率降低,差异具有统计学意义。单因素及多因素分析的结果表明,腹腔镜的手术方式是保证ERAS实施效果的独立保护性因素(OR:0.33,95%CI=0.22~0.47,P=0.005),而术前CRP水平则是影响ERAS实施效果的独立危险因素(OR:3.97,95%CI=3.26~4.35,P=0.016)。结论:本研究再次证实在结直肠外科中,ERAS方案的实施可以缩短病人术后排气时间、术后住院时间以及术后ICU住院时间。同时,ERAS可以降低术后I-II级并发症的发生。并且对于结直肠手术病人,腹腔镜的应用以及术前病人CRP水平关系到ERAS的实施效果。

关键词：加速康复外科结直肠癌影响因素效果评价

Laser assisted phase transformation of carbon-based transition metal oxide electrode for high-performance supercapacitor application

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Science China Chemistry 2025年

作者： Mingzhen Xiu Jianghong zhang Yu Lu Xun Cao Kang Huang Siyu Zhu Bowei zhang Yu Liang Chunwang zhao Junsheng Wu Yizhong Huang School of Materials Science and Engineering Nanyang Technological University Energy Research Institute Interdisciplinary Graduate Programme Nanyang Technological University Institute of Sustainability for Chemicals Energy and Environment Agency for Science Technology and ResearchSingapore Institute of Advanced Materials and Technology University of Science and Technology Beijing School of Materials Science and Technology Shenyang University of Chemical Technology School of Materials Science and Hydrogen Energy Foshan University

Transitional metal oxides have a wide range of applications due to their superior *** enhanced performance of metal oxide relies on their desirable oxidation states,which is challenging to achieve and requires complex *** study demonstrates a facile strategy enabling rapid phase transformation of transition metal *** approach involves synthesis of a tetragonal Mn3O4/carbon cloth composite using the liquid plasma discharge deposition （LPDD） technique,followed by laser irradiation with the presence of ethylene glycol under an N2gas *** resulting face-centered cubic MnO/carbon cloth electrode exhibits an excellent capacitance of 1,125 F g-1at 2 A g-1,with remarkable capacitance retention of 95%after10,000 *** approach can be extended to other transition metals including Co,Cu,Cr,Fe,Ni,and *** simulations reveal the significance of ethylene glycol and the underlying mechanism associated with the phase transformation of transition metal *** present study offers a powerful technique for the phase transformation of transition metal oxide which can guide the development of new electrode materials with enhanced electrochemical properties for high-performance energy storage applications.

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