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Exercise combined with administration of adipose-derived stem cells ameliorates neuropathic pain after spinal cord injury

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Neural Regeneration Research 2023年第8期18卷 1841-1846页

作者： Xing Cheng Gu-Ping Mao wen-Jie Hu Zheng-Ran Yu Yi-Yang Xu Wei Chen Xiang Li Xiao-Lin Zeng wen-wu zhang Jie-wen Chen Yong Wan Le Wang Department of Spine Surgery Orthopedic Research InstituteThe First Affiliated Hospital of Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Orthopedics and TraumatologyGuangzhouGuandong ProvinceChina Department of Joint Surgery and Sport Medicine Orthopedic Research InstituteThe First Affiliated Hospital of Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Orthopedics and TraumatologyGuangzhouGuandong ProvinceChina Department of Rehabilitation Medicine The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuandong ProvinceChina Department of Spine Surgery Orthopedics Center of Guangdong Provincial People’s Hospital and Guangdong Academy of Medical SciencesGuangzhouGuandong ProvinceChina State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhouGuandong ProvinceChina

Experimental studies have shown that exercise and human adipose-derived stem cells(ADSCs)play positive roles in spinal cord injury(SCI).However,whether ADSCs and/or exercise have a positive effect on SCI-induced neuropathic pain is still ***,there is a need to explore the effects of exercise combined with administration of ADSCs on neuropathic pain after *** this study,a thoracic 11(T11)SCI contusion model was established in adult C57BL/6 *** was initiated from 7 days post-injury and continued to 28 days post-injury,and approximately 1×105 ADSCs were transplanted into the T11 spinal cord lesion site immediately after *** function and neuropathic pain-related behaviors were assessed weekly using the Basso Mouse Scale,von Frey filament test,Hargreaves method,and cold plate *** studies(Eriochrome cyanine staining and immunohistochemistry)were performed at the end of the experiment(28 days post-injury).Exercise combined with administration of ADSCs partially improved early motor function(7,14,and 21 days postinjury),mechanical allodynia,mechanical hypoalgesia,thermal hyperalgesia,and thermal *** of ADSCs reduced white and gray matter loss at the lesion *** addition,fewer microglia and astrocytes(as identified by expression of ionized calcium-binding adapter molecule 1 and glial fibrillary acidic protein,respectively)were present in the lumbar dorsal horn in the SCI+ADSCs and SCI+exercise+ADSCs groups compared with the sham *** findings suggest that exercise combined with administration of ADSCs is beneficial for the early recovery of motor function and could partially ameliorate SCIinduced neuropathic pain.

关键词： adipose-derived stem cells allodynia exercise glial fibrillary acidic protein hyperalgesia hypoalgesia ionized calcium-binding adapter molecule 1 motor function spinal cord injury

Research of multilayers in EUV, soft X-ray and X-ray

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光学精密工程 2005年第4期13卷 512-518页

作者： WANG Zhan-shan WANG Feng-li zhang Zhong WANG Hong-chang wu wen-juan zhang Shu-min XU Yao GU Zhong-xiang CHENG Xin-bin L1 Cun-xia wu Yong-rong WANG Bei QIN Shu-jin CHEN Ling-yan Institute of Precision Optical Engineering Department of Physics Tongji University Shanghai 200092 China

To develop beam splitters for soft X-ray laser Michelson interferometer at 13.9 nm, Mo/Si multilayers of 100 nm thickness deposited on both sides of silicon nitride were fabricated by using DC magnetron sputtering. Initial evaluation of their reflectivity and transmission showed that reflectivity and transmission were above 10% and 25%. The broadband analyzers have been designed, fabricated and characterized for 13～20 nm polarization measurements. The measured results are in good agreement with the design. The supermirrors with different angular intervals at 0.154 nm have been designed, fabricated and characterized.

关键词： EUV X射线多层性激光宽带光纤

Delay of ZGA initiation occurred in 2-cell blocked mouse embryos

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Cell Research 2003年第3期13卷 179-185页

作者： JIA JING QIU,wu wen zhang,ZHI Li wu,YI HONG WANG,MIN QIAN,YI PING LIInstitute,of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences. Chinese Academy of Sciences,200031 Shanghai,ChinaSchool of Life Science,East China Normal University. 200062 Shanghai,China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 200031 Shanghai China

One-cell mouse embryos from KM strain and B6C3F1 strain were cultured in M16 medium,in which2-cell block generally *** of KM strain exhibited 2-cell block,whereas B6C3F1 embryos,which are regarded as a nonblocking strain,proceeded to the 4-cell stage in our culture *** is oftenassumed that the block of early development is due to the failure of zygotic gene activation（ZGA）in *** this study we examined protein synthesis patterns by two-dimensional gel electrophoresis of[35S]methionine radiolabeled 2-cell *** from the blocking strain and the nonblocking strainwere compared in their development both in vitro and in *** detection of TRC expression,a markerof ZGA,at 42 h post hCG in KM embryos developed in vitro suggested that ZGA was also initiated even inthe 2-cell arrested ***,a significant delay of ZGA was observed in KM strain as comparedwith normally developed B6C3F1 *** the very beginning of major ZGA as early as 36 h post

关键词： monse embryo two-cell block zygotic gone activation (ZGA).

Expression and functional identification of the hypothetical adhesin P32 from Mycoplasma genitalium

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Journal of Microbiology and Immunology 2006年第3期4卷 200-206页

作者： LIN BO LI YI MOU wu wen BO zhang MIN JUN YU Institute of Pathogenic Biology Medical College Nanhua University Hengyang P. R. China

Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid sequence with adhesin protein P30 from M. pneumoniae. Since M. pneumoniae mutants lacking P30 protein is defective in cytadherence, P32 protein has been proposed to be an essential adhesin implicated in the adherence of M. genitaliurn to host cells. The prokaryotic expression vector pET-30 （＋）/p32 was constructed in the present study, and the recombinant protein was expressed in E. coli and purified under denaturing condition. As demonstrated by the immuno- blotting analysis, the recombinant protein could react with rabbit antisera against M. genitalium, and adherence inhibition assays were performed with antisera against this recombinant protein. It was demonstrated that P32 protein apperared to be an adhesion protein of M. genitalium, thus providing the experimental basis for better understanding of the pathogenesis of M. genitalium infection and for the development of the related vaccines against the infection.

关键词： Mycoplasma genitaliurn Adhesin P32 Recombinant protein Expression Functional identification

The Generation of Eukaryotic Expression Vectors of shRNA Specific for Stat6

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生物医学工程学杂志 2005年第S1期22卷 73-74页

作者： Ming-Sheng zhang Yun-Feng ZHOU~Δ Zhi-Guo LUO Jian-Ping wu wen Jie zhang(Department of Radio-Chemotherapy, Zhongnan Hospital, Cancer Research Center, wuhan University,wuhan 430071, China)

Trends of alkaline phosphatase to prealbumin ratio in patients with hepatitis B linked to hepatocellular carcinoma development

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World Journal of Gastroenterology 2025年第2期31卷 42-51页

作者： wen-Chong Zhen Jing Sun Xue-Ting Bai Qian zhang Zi-Han Li Yi-Xin zhang Rong-Xuan Xu Wei wu Zhi-Han Yao Chun-wen Pu Xiao-Feng Li School of Public Health Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Graduate School Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Dalian Public Health Clinical Center Dalian Municipal Research Institute for Public HealthDalian 116001Liaoning ProvinceChina Department of Epidemiology and Health Statistics Dalian Medical UniversityDalian 116044Liaoning ProvinceChina

BACKGROUND Chronic hepatitis B often progresses silently toward hepatocellular carcinoma(HCC),a leading cause of mortality *** detection of HCC is crucial,yet *** To investigate the role of dynamic changes in alkaline phosphatase to prealbumin ratio(APR)in hepatitis B progression to *** Data from 4843 patients with hepatitis B(January 2015 to January 2024)were *** incidence rates in males and females were compared using the log-rank *** were evaluated using Kaplan–Meier *** Linear Mixed-Effects Model was applied to track the fluctuation of APR levels over ***,Joint Modeling of Longitudinal and Survival data was employed to investigate the temporal relationship between APR and HCC *** The incidence of HCC was higher in *** ensure the model’s normality assumption,this study applied a logarithmic transformation to APR,yielding *** levels were higher in females(t=5.26,P<0.01).A 1-unit increase in ratio correlated with a 2.005-fold higher risk of HCC in males(95%CI:1.653-2.431)and a 2.273-fold higher risk in females(95%CI:1.620-3.190).CONCLUSION Males are more prone to HCC,while females have higher APR *** no baseline APR link,rising APR indicates a higher HCC risk.

关键词： Alkaline phosphatase to prealbumin ratio Chronic hepatitis B patients Hepatocellular carcinoma Retrospective cohort study Linear Mixed-Effect Mode Joint Modelling of Longitudinal and Survival data

MAGE-A4在肿瘤诊断与免疫治疗中的作用研究进展

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解放军医学杂志 2023年第7期48卷 834-840页

作者：吴文章张凡贾祺李玉民兰州大学第二医院普外科兰州730030 甘肃省消化系统肿瘤重点实验室兰州730030

肿瘤免疫治疗已成为继放化疗、靶向治疗后最终攻克癌症的希望之一,而肿瘤相关性抗原是免疫治疗的重要靶点。黑色素瘤相关抗原-A4(MAGE-A4)作为癌睾丸抗原,是MAGE-A亚家族的重要成员,具有很好的组织学特异性,在多种肿瘤组织中呈高表达,... 详细信息

肿瘤免疫治疗已成为继放化疗、靶向治疗后最终攻克癌症的希望之一,而肿瘤相关性抗原是免疫治疗的重要靶点。黑色素瘤相关抗原-A4(MAGE-A4)作为癌睾丸抗原,是MAGE-A亚家族的重要成员,具有很好的组织学特异性,在多种肿瘤组织中呈高表达,在正常组织(除睾丸、胎盘外)中均呈低表达。MAGE-A4参与细胞功能的调节,具有调控细胞周期、诱导细胞分化和生长、参与上皮-间质转化等重要作用,与肿瘤的发生、发展、转移及预后密切相关。目前,以MAGE-A4为靶点的T细胞受体转基因T细胞(TCR-T)免疫治疗,其Ⅰ期临床试验结果显示总体反应率(ORR)为23.7%、病情稳定率为47.4%,Ⅱ期临床试验正在开展,提示以MAGE-A4为靶点的TCR-T免疫治疗具有较高的临床应用价值。本文总结了MAGE-A4的结构和生物学功能及其在多种实体肿瘤中的最新研究进展和临床应用,旨在为MAGE-A4的临床转化及免疫靶向治疗提供理论基础。

关键词：黑色素瘤相关抗原-A4 结构功能恶性肿瘤免疫治疗

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Real-world evidence on the efficacy and safety of vonoprazanamoxicillin dual therapy for Helicobacter pylori treatment in elderly patients

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World Journal of Gastroenterology 2025年第1期31卷 68-78页

作者： wen Gao Jing-wen Li Hui Ye Xue-Zhi zhang Jian-Xiang Liu Hong Cheng Department of Gastroenterology Peking University First HospitalBeijing 100034China School of Medicine Tsinghua MedicineTsinghua UniversityBeijing 100034China Department of Traditional Chinese Medicine and Integrative Medicine Peking University First HospitalBeijing 100034China

BACKGROUND A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of Helicobacter pylori(***).While substantial research supports the efficacy and safety of vonoprazan and amoxicillin(VA)dual therapy in the general population,there is still a lack of studies specifically focusing on its safety in elderly *** To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for *** in elderly *** As a real-world retrospective study,data were collected from elderly patients aged 60 years and above who accepted VA dual therapy(vonoprazan 20 mg twice daily+amoxicillin 1000 mg thrice daily for 14 days)for *** eradication in the Department of Gastroenterology at Peking University First Hospital between June 2020 and January *** status was evaluated by^(13)C-urease breath test 6 weeks after *** adverse events(AEs)during treatment were *** In total,401 cases were ***-one cases were excluded due to loss to follow-up,lack of re-examination,or unwillingness to take *** total of 380 included cases comprised 250 who received VA dual therapy as first-line treatment and 130 who received VA dual therapy as rescue *** was successfully eradicated in 239 cases(95.6%)in the first-line treatment group and 116 cases(89.2%)in the rescue treatment *** overall incidence of AEs was 9.5%for both ***,9.2%of patients experienced an AE in the first-line treatment group and 10.0%in the rescue treatment *** patients discontinued treatment due to AE,with a discontinuation rate of 1.3%.No serious AE *** The VA dual therapy regimen as a first-line treatment and a rescue therapy was effective and safe for elderly patients aged 60 and older.

关键词： Helicobacter pylori Vonoprazan Amoxicillin Dual therapy Elderly

Return to work in young and middle-aged colorectal cancer survivors:Factors influencing self-efficacy,fear,resilience,and financial toxicity

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World Journal of Gastroenterology 2025年第1期31卷 79-92页

作者： Dan Hu Yue Li Hua zhang Lian-Lian Wang wen-wen Liu Xin Yang Ming-Zhao Xiao Hao-Ling zhang Juan Li Department of Nursing The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Nursing National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Gastrointestinal Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Biomedical Science Advanced Medical and Dental InstituteUniversity Sains MalaysiaPenang 13200Malaysia Academic Affairs Office The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Return to work(RTW)serves as an indication for young and middle-aged colorectal cancer(CRC)survivors to resume their normal social ***,these survivors encounter significant challenges during their RTW ***,scientific research is necessary to explore the barriers and facilitating factors of returning to work for young and middle-aged CRC *** To examine the current RTW status among young and middle-aged CRC survivors and to analyze the impact of RTW self-efficacy(RTW-SE),fear of progression(FoP),eHealth literacy(eHL),family resilience(FR),and financial toxicity(FT)on their RTW *** A cross-sectional investigation was adopted in this *** September 2022 to February 2023,a total of 209 participants were recruited through a convenience sampling method from the gastrointestinal surgery department of a class A tertiary hospital in *** investigation utilized a general information questionnaire alongside scales assessing RTW-SE,FoP,eHL,FR,and *** analyze the factors that influence RTW outcomes among young and middle-aged CRC survivors,Cox regression modeling and Kaplan-Meier survival analysis were *** A total of 43.54%of the participants successfully returned to work,with an average RTW time of 100 *** regression univariate analysis revealed that RTW-SE,FoP,eHL,FR,and FT were significantly different between the non-RTW and RTW groups(P<0.05).Furthermore,Cox regression multivariate analysis identified per capita family monthly income,job type,RTW-SE,and FR as independent influencing factors for RTW(P<0.05).CONCLUSION The RTW rate requires further *** levels of RTW-SE and FR were found to significantly increase RTW among young and middle-aged CRC *** professionals should focus on modifiable factors,such as RTW-SE and FR,to design targeted RTW support programs,thereby facilitating their timely reintegration into mainstream society.

关键词： Return to work Colorectal neoplasms Return-to-work self-efficacy Fear of progression Family resilience Financial toxicity