检索结果-南通市图书馆

Sodium alginate coating simultaneously increases the biosafety and immunotherapeutic activity of the cationic mRNA nanovaccine

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2023年第3期13卷 942-954页

作者： Xing Duan Yi Zhang Mengran Guo Na Fan Kepan Chen Shugang Qin wen Xiao Qian Zheng Hai Huang Xiawei Wei Yuquan Wei Xiangrong Song Department of Critical Care Medicine Department of Clinical PharmacyFrontiers Science Center for Disease-related Molecular NetworkState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610041China West China Hospital Sichuan UniversityChengdu 610041China

The extraordinary advantages associated with mRNA vaccines,including their high efficiency,relatively low severity of side effects,and ease of manufacture,have enabled them to be a promising immunotherapy approach against various infectious diseases and ***,most mRNA delivery carriers have many disadvantages,such as high toxicity,poor biocompatibility,and low efficiency in vivo,which have hindered the widespread use of mRNA *** further characterize and solve these problems and develop a new type of safe and efficient mRNA delivery carrier,a negatively charged SA@DOTAP-mRNA nanovaccine was prepared in this study by coating DOTAP-mRNA with the natural anionic polymer sodium alginate(SA).Intriguingly,the transfection efficiency of SA@DOTAP-mRNA was significantly higher than that of DOTAP-mRNA,which was not due to the increase in cellular uptake but was associated with changes in the endocytosis pathway and the strong lysosome escape ability of SA@*** addition,we found that SA significantly increased the expression of LUC-mRNA in mice and achieved certain spleen ***,we confirmed that SA@DOTAP-mRNA had a stronger antigen-presenting ability in E.G7-OVA tumor-bearing mice,dramatically inducing the proliferation of OVA-specific CLTs and ameliorating the antitumor ***,we firmly believe that the coating strategy applied to cationic liposome/mRNA complexes is of potential research value in the field of mRNA delivery and has promising clinical application prospects.

关键词： Negatively charged mRNA vaccine Sodium alginate Cationic liposome DOTAP Lysosomes escape Cancer Immunotherapy Toxicity

复发性慢性硬膜下血肿脑膜中动脉影像学特征及介入栓塞治疗

在线全文

学校读者我要写书评

暂无评论

介入放射学杂志 2024年第4期33卷 413-418页

作者：刘振生魏庭丰孙勇匡雄伟王友伟吴臣义段晓春齐文涛彭爱军张文扬州大学附属医院介入放射科江苏扬州225001 扬州大学附属医院神经外科江苏扬州225001 扬州大学附属医院超声科江苏扬州225001

目的初步评估复发性慢性硬膜下血肿(chronic subdural hematoma,CSDH)脑膜中动脉形态学和血流动力学特征,及脑膜中动脉栓塞(middle meningeal artery embolization,MMAE)治疗疗效。方法回顾性分析我院2019年5月至2022年12月期间31例(15.2%)外科钻孔引流术后复发并接受MMAE治疗的20例单侧CSDH患者的临床及影像资料。于3D-TOF MRA测量患侧MMA直径、相对血流速度,并与随机抽取同期外科钻孔引流术后未复发的20例CSDH患者作为对照。随访观察MMAE术后影像学结果、临床症状改善及并发症等。结果复发组患侧MMA平均直径(1.55±0.45)mm明显大于对照组(1.32±0.36)mm,两组差异有统计学意义(t=2.118,P=0.028)。复发组相对血流速度(SI比值均数,1.45±0.23)明显大于对照组(SI比值均数,1.23±0.26),两组差异有统计学意义(t=3.493,P=0.001)。复发组血肿外膜强化分级评分(2.25±0.55)分,对照组(1.60±0.68)分,两组差异有统计学意义(t=3.322,P=0.002)。MMAE术后影像学随访血肿完全吸收19例(95%),其中3个月完全吸收8例(40%),6个月完全吸收16例(95%),中位吸收时间16周(IQR:7~22)。除1例复发症状反复外,余患者临床症状均不同程度改善,所有患者均未见手术相关并发症。结论与非复发者相比,外科术后复发侧血肿外膜血供更丰富、MMA管径更粗、血流速度更快,MMAE是治疗此类CSDH安全有效的方法。

关键词：慢性硬膜下血肿复发脑膜中动脉血管造影栓塞

污水处理厂废水排放对近海抗生素污染的贡献:以杭州湾为例

在线全文

学校读者我要写书评

暂无评论

环境工程 2024年第4期42卷 1-8页

作者：李菲菲苏志国曹丰母清林黄备陈吕军温东辉清华大学环境学院北京100084 北京大学环境科学与工程学院北京100087 浙江省海洋生态环境监测中心浙江舟山316021

以污染形势严峻的东海杭州湾(HB)为研究对象,采用超高效液相色谱质谱联用仪,探究6类77种抗生素在污水处理厂、近岸纳污区和近海地区的组成和分布规律。检测结果显示,污水处理厂对抗生素的去除率介于31.1%~88.8%,其中对喹诺酮类抗生素去... 详细信息

以污染形势严峻的东海杭州湾(HB)为研究对象,采用超高效液相色谱质谱联用仪,探究6类77种抗生素在污水处理厂、近岸纳污区和近海地区的组成和分布规律。检测结果显示,污水处理厂对抗生素的去除率介于31.1%~88.8%,其中对喹诺酮类抗生素去除率最高,平均为80.3%。污水厂出水排入相应的近岸纳污区,嘉兴(JX)纳污区检出53种抗生素,平均浓度为48180.7 ng/L,上虞(sy)纳污区检出63种抗生素,平均浓度为341.2 ng/L。主成分分析结果表明,污水处理厂是sy纳污区抗生素污染的重要来源,而JX纳污区抗生素受污水处理厂排水的影响小,明显受到含有兽用抗生素的其他污染源影响。近岸纳污区汇集了含抗生素废水后,成为近海抗生素污染的“源”。近海检出51种抗生素,平均浓度达189.5 ng/L。抗生素浓度从近岸向近海扩散存在明显的“距离衰减”趋势,尤其以喹诺酮类抗生素的衰减幅度最大。

关键词：抗生素污水处理厂近岸纳污区近海杭州湾

纤维蛋白原诱导SH-sy5Y细胞凋亡和损伤的机制研究

在线全文

学校读者我要写书评

暂无评论

卒中与神经疾病 2024年第3期31卷 236-241页

作者：文婷婷孟兰霞但亮朱柯东罗佳颖张振涛张兆辉武汉大学人民医院神经内科 430060

目的探讨纤维蛋白原(Fibrinogen,Fg)对人神经母细胞瘤细胞(Human neuroblastoma cells,SH-sy5Y)的影响及其机制。方法采用不同水平的纤维蛋白原(0、2、4、8 mg/mL)处理SH-sy5Y细胞,并使用了多种实验方法来评估其对细胞的影响:CCK8试剂盒... 详细信息

目的探讨纤维蛋白原(Fibrinogen,Fg)对人神经母细胞瘤细胞(Human neuroblastoma cells,SH-sy5Y)的影响及其机制。方法采用不同水平的纤维蛋白原(0、2、4、8 mg/mL)处理SH-sy5Y细胞,并使用了多种实验方法来评估其对细胞的影响:CCK8试剂盒(Cell counting kit-8 viability assay)检测细胞活力;Hoechst33342/碘化丙啶(Propidine iodide,PI)染色试剂盒观察细胞凋亡情况;细胞色素c氧化酶IV(Cytochrome C oxidase IV,COX IV)细胞免疫荧光染色检测线粒体功能损伤;蛋白免疫印迹法检测线粒体凋亡通路相关指标[B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)、Bcl2关联X蛋白(Bcl2 associated X protein,Bax)、细胞色素C(Cytochrome C,Cyt-C)、活化半胱胺酸蛋白酶蛋白-3(Activated caspase-3)]的表达水平。结果SH-sy5Y细胞暴露于纤维蛋白原会显著降低细胞活力,触发神经元凋亡,诱导线粒体功能障碍。此外,Western blot分析发现纤维蛋白原处理也改变了SH-sy5Y细胞中线粒体通路凋亡相关指标(Bcl-2,Bax,Cytochrome C,Activated caspase-3)的表达水平。结论纤维蛋白原引起了SH-sy5Y细胞的损伤和凋亡,其作用机制可能涉及线粒体功能障碍。

关键词：纤维蛋白原 SH-sy5Y细胞细胞凋亡线粒体功能障碍

A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2013年第3期29卷 348-354页

作者： Guang-Fei Deng Shu-Jing Liu Xun-Sha Sun Wei-wen Sun Qi-Hua Zhao Wei-Ping Liao Yong-Hong Yi Yue-Sheng Long Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes Guangzhou Medical University

LIM kinase 1 （LIMK1）, a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI （8.0） software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt ＋884 to ＋966 in the human LIMK1 3＇ untranslated region （UTR） was highly conserved in the mouse Limkl 3＇ UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-sy5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-sy5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3＇ UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3＇-UTR mutant and neuroloqical disorders.

关键词： LIM kinase 1 region post-transcriptsecondary structurethree-prime untranslatedonal regulation RNA

The Constitute of Thick Tight Sandstone Belt and the Main Controlling Factors of Effective Reservoir

在线全文

学校读者我要写书评

暂无评论

Acta Geologica Sinica(English Edition) 2015年第A1期89卷 184-187页

作者： FAN Guangjuan WANG Yongchao Ma Shizhong YAN Baiquan wen Huijian ZHAO Yuejun Northeast Petroleum University College ofGeosciences Daqing Heilongjiang 163318 China Unconventional Oil and Gas Accumulation and Development of Provincial Department of Construction of State KeyLaboratory Daqing Heilong jiang 163318 China Northeast Petroleum University College of Petroleum Engineering Daqing Heilong/iang 163318 China

1 Introduction The western 3D region of the Sulige gas field is located in the Yi-shan slope north of northern Su47 and south of Su48 medium sand belt of the Ordos Basin, the area of it is about 431 km2. The average thickness of He8 is 69.1m, the thickness of it is 83.9m; The effective sand thickness is 14.4 m, the thickness of it is 21.5m.

关键词： SANDSTONE RESERVOIRS SEDIMENTARY rocks SEDIMENTARY structures HYDRAULIC structures CHINA

Combined endovascular brachytherapy, sorafenib, and transarterial chemobolization therapy for hepatocellular carcinoma patients with portal vein tumor thrombus

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第43期23卷 7735-7745页

作者： Zi-Han Zhang Qing-Xin Liu wen Zhang Jing-Qin Ma Jian-Hua Wang Jian-Jun Luo Ling-Xiao Liu Zhi-Ping Yan Department of Interventional Radiology Zhongshan HospitalFudan University and Shanghai Institute of Medical Imaging

AIM To evaluate the safety and efficacy of combined endovascular brachytherapy(EVBT),transarterial chemoembolization(TACE),and sorafenib to treat hepatocellular carcinoma(HCC) patients with main portal vein tumor thrombus(MPVTT).METHODS This single-center retrospective study involved 68 patients with unresectable HCC or those who were unfit for liver transplantation and percutaneous frequency ablation according to the BCLC classification. All patients had Child-Pugh classification grade A or B,Eastern Cooperative Oncology Group(ECOG)performance status of 0-2,and MPVTT. The patients received either EVBT with stent placement,TACE,and sorafenib(group A,n = 37),or TACE with sorafenib(group B,n = 31). The time to progression(TTP) and overall survival(OS) were evaluated by propensity score *** In the entire cohort,the 6-,12-,and 24-mo survival rates were 88.9%,54.3%,and 14.1% in group A,and 45.8%,0%,and 0% in group B,respectively(P < 0.001). The median TTP and OS were significantly longer in group A than group B(TTP: 9.0 mo vs 3.4 mo,P < 0.001; OS: 12.3 mo vs 5.2 mo,P < 0.001). In the propensity score-matched cohort,the median OS was longer in group A than in group B(10.3 mo vs 6.0 mo,P < 0.001). Similarly,the median TTP was longer in group A than in group B(9.0 mo vs 3.4 mo,P < 0.001). Multivariate Cox analysis revealed that the EVBT combined with stent placement,TACE,and sorafenib strategy was an independent predictor of favorable OS(HR = 0.18,P < 0.001). CONCLUSION EVBT combined with stent placement,TACE,and sorafenib might be a safe and effective palliative treatment option for MPVTT.

关键词： Hepatocellular carcinoma Transarterial chemoembolization Endovascular brachytherapy Main portal vein tumor thrombus Sorafenib

Melatonin ameliorated okadaic-acid induced Alzheimer-like lesions

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第3期25卷 22-26页

作者： Yi-peng WANG,Xian-tao LI,Shi-jie LIU,Xin-wen ZHOU,Xiao-chuan WANG,Jian-zhi WANG2 TongjiMedical College,Huazhong University of Science and Technology,Wuhan 430030,China Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

AIM: To explore the protective effects of melatonin (Mel) on the abnormal phosphorylation of neuronal cytoskeletal proteins. METHODS: We generated a neuroblastoma (SH-sy5Y) cell system in which cytoskeletal proteins are abnormally phosphorylated resulting in microtubule disruption due to the marked inhibition of protein phosphatase activities by okadaic acid (OA). RESULTS: OA-induced declines in cell viability and mitochondrial metabolic activity were remarkably prevented by Mel. In addition, the hyperphosphorylation/accumulation of neurofilament- (NF-) H/M subunits and the disruption of microtubules, induced by OA, were significantly inhibited by Mel. CONCLUSION: Our results suggest multiple protective functions of Mel against a series of pathological lesions known to culminate in AD, including abnormal phosphorylation of cytoskeletal proteins, microtubule disassembly and mitochondrion-initiated cell toxicity.

关键词： Alzheimer disease protein phosphatase okadaic acid neurofilament proteins melatonin

Mono-2-ethylhexyl Phthalate Promotes Migration and Invasion by Regulating the Epithelial-Mesenchymal Transition in SHsy5Y Cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第3期35卷 276-281页

作者： XU Qi ZHANG Bo LI Xu YE Jia Ming HUO Chuan Yi YIN Jian Li ZHANG Ru Xuan ZHAO Wei Sen QI wen YE Lin Department of Occupational and Environmental Health School of Public HealthJilin UniversityChangchun 130000JilinChina Department of Pediatric Neurology the First Hospital of Jilin UniversityChangchun 130000JilinChina

Neuroblastoma(NB)is the most common extracranial solid tumor of *** accounts for 8%–10%of all malignant tumors in children^([1])and results in a heavy burden to the life and health of *** is a complex disease,and its... 详细信息

关键词： SHsy5Y Transition malignant