检索结果-南通市图书馆

Insect Science 2019年第3期26卷 441-452页

作者： Wei-wen Chen Kui Kang Pan Yang wen-qing zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen University Guangzhou China

In insects, the gustatory system plays a crucial role in multiple physiological behaviors, including feeding, toxin avoidance, courtship, mating and oviposition. Gustatory stimuli from the environment are recognized by gustatory receptors. To date, little is known about the function of gustatory receptors in agricultural pest insects. In this study, we cloned a sugar gustatory receptor gene, N/Gr11, from the brown planthopper (BPH), Nilaparvata lugens (Stal), a serious pest of rice in Asia;we then identified its ligands, namely, fructose, galactose and arabinose, by calcium imaging assay. After injection of N/Gr11 doublestranded RNA, we found that the number of eggs laid by BPH decreased. Moreover, we found that N/Gr11 inhibited the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and promoted the phosphorylation of protein kinase B (AKT). These findings demonstrated that N/Gr11 could accelerate the fecundity of BPH through AMPK- and AKT-mediated signaling pathways. This is the first report to indicate that a gustatory receptor modulates the fecundity of insects and that the receptor could be a potential target for pest control.

关键词： brown planthopper fecundity gustatory receptors ligands sugar

Galactose-N/Gr11 inhibits AMPK phosphorylation byactivating the PI3K-AKT-PKF-ATPsignaling cascade viainsulin receptor and Gβγ

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2021年第3期28卷 735-745页

作者： Wei-wen Chen Kui Kang Jun Lv Lei Yue wen-qing zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen UniversityGuangzhouChina

As ligands of the sugar gustatory receptors,sugars have been known to activatethe insulin/insulin-like growth factor signaling pathway;however,the precise pathways thatare activated by the sugar-bound gustatory receptors in insects remain *** this study,we aimed to investigate the signaling cascades activated by NlGr11,a sugar gustatory re-ceptor in the brown planthopper Nilaparvata lugens(Stal),and its ***-boundNGrl1(galactose-NIGr11)activated the-phosphatidylinositol 3-kinase(PI3K)-AKT signaling cascade via insulin receptor(InR)and Gβγin *** addition,galactose-NlGr11inhibited the adenosine monophosphate-activated protein kinase(AMPK)phosphorylationby activating the AKT-phosphofructokinase(PFK)-ATP signaling cascade in ***-tantly,the InR-PI3K-AKT-PFK-AKT signaling cascade was activated and the AMPKphosphorylation was inhibited after feeding the brown planthoppers with galactose ***,these findings confirm that NIGr1l can inhibit AMPK phosphorylationby activating the PI3K-AKT-PFK-ATP signaling cascades via both InR and GBy whenbound to ***,our study provides novel insights into the signaling pathwaysregulated by the sugar gustatory receptors in insects.

关键词： AMPK galactosc Gβγ IlS pathway sugar gustatory receptor

First-principles study on the structure, electronic and magnetic properties of HoSin （n=1-12, 20） clusters

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of physics 2014年第2期9卷 210-218页

作者： Tai-Gang Liu wen-qing zhang Yan-Li Li School of Life Science and Technology Xinxiang Medical University Xinxiang 453003 China School of Mechanics and Electronics Henan Institute of Science and Technology Xinxiang 453003 China Department of Physics Wuhan University of Technology Wuhan 430070 China

The structure, electronic and magnetic properties of HoSin（n= 1 - 12, 20） clusters have been widely investigated by first-principles calculation method based on density flmctional theory （DFT）. From our calculation results, we find that for HoSin（n=1- 12） clusters except n = 7.10, the most stable structures are a replacement of Si atom in the corresponding pure Sin＋1 clusters by Ho atom. The doping of Ho atom makes the stability of Si clusters enhance remarkably, and HoSin（n = 2, 5, 8, 11） clusters are more stable than their neighboring clusters. The magnetic moment of Ho atom in HoSin （n = 1 - 12, 20） clusters mainly comes from of electron of tto, and never quenches.

关键词： structure stability electronic and magnetic properties HoSin cluster

Morph-specific differences in metabolism related to flight in the wing-dimorphic Aphis gossypii

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2010年第6期17卷 527-534页

作者： Sheng-Li Shi Xiao-Xia Liu qing-wen zhang zhang-Wu Zhao Department of Entomology College of Agriculture and Biotechnology China Agricultural University Beijing China Institute of Food Crops Yunnan Academy of Agricultural Sciences Kunming China

The cotton aphid, Aphis gossypii Glover, is a wing-dimorphic species, which causes globally important agricultural losses. In this present study, we compared the biochemical basis of wing polymorphism in A. gossypii with respect to trade-off of energy resources, including glycogen, trehalose, lipids （total lipid, triglyceride and phospholipid）, free fatty acids, and soluble protein between dispersal and reproduction morphs during the wing-bud nymph and adulthood. Total lipid, triglyceride and free fatty acids were significantly higher in winged versus wingless morphs at 12 h of adulthood, the period during which alates are able to fly. By contrast, the wingless morph contained more glycogen than the winged morph from the 4th nymphal stage to adulthood. Trehalose content in the wingless morph was also higher than that in the winged morph during the 3rd and 4th nymphal stages, but vice versa at 12 h of adulthood. Finally, soluble protein content increased from nymphs to adults and was higher during adulthood in aptera versus alate. Whole-body water content in 12-h adults was significantly higher in apterae than that in alatae. These results indicate significant physiological differences between morphs related to specialization for flight.

关键词： Aphis gossypii, lipids, proteins trade-off, wing dimorphism

Two New Oleanane Saponins from Anemone anhuiensis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2000年第8期11卷 697-700页

作者： qing wen zhang wen Cai YE Chun Tao CHE Shou Xun ZHAO Department of Phytochemistry China Pharmaceutical UniversityNanjing 210009 School of Chinese Medicine The Chinese University of Hong KongHong Kong

Two new oleanane triterpene saponins, named as anhuienside C (1) and D (2), were isolated from the rhizomes of Anemone anhuiensis (Ranunculaceae). The structures of the new compounds were elucidated as 3-O-alpha-L-rhamnopyranosyl (1-->2)-beta-D-xylopyranosyl oleanolic acid 28-O-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl ester (1) and 3-O-beta-D-xylopyranosyl oleanolic acid 28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl ester (2), respectively.

关键词： anemone anhuiensis ranunculaceae anhuiensides C and D oleanane saponin

Ligand dose-dependent activation of signaling pathways through the gustatory receptor NlGr11 linked to feeding efficacy in Nilaparvata lugens

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2023年第5期30卷 1420-1430页

作者： Wei-wen Chen Kai Lin Jun Lv Qin Su Meng-Yi zhang Kui Kang wen-qing zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen UniversityGuangzhouChina College of Biology and Agriculture Zunyi Normal UniversityZunyiGuizhou ProvinceChina

Insects often face both conditions with sufficient nutrients and conditions of undernutrition in the *** gustatory receptors,insects sense nutrients and regulate their physiological functions such as feeding and ***,it remains unclear whether signaling pathways activated by gustatory receptors depend on the concentration of nutrients and whether the difference in signaling pathways directly affects insects’physiological ***,we found that a sugar gustatory receptor,NlGr11,from the brown planthopper(BPH),Nilaparvata lugens,activated G protein-coupled signaling and ionotropic pathways when bound to high galactose *** subsequently demonstrated longer feeding times,feeding loads,and higher vitellogenin(NlVg)expression than BPHs exposed to high galactose concentrations,which only activated the ionotropic *** the first time,our findings link plant nutrient conditions,signaling pathways activated by nutrients,and their gustatory receptors,and nutrient dose-dependent feeding efficacy and vitellogenin(Vg)expression in an *** will help us to better understand the molecular mechanism for insect feeding strategies on plants at different stages of nutritional conditions.

关键词： feeding efficacy galactose G protein-coupled signaling pathway insect gustatory receptor ionotropic pathway

Effects of Carrier Gas on Carbon Incorporation in GaN

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics Letters 2014年第3期31卷 122-124页

作者： zhang Jian-Li LIU Jun-Lin PU Yong FANG wen-qing zhang Meng JIANG Feng-Yi National Engineering and Technology Research Center for LED on Si Substrate Nanchang University Nanchang 330047

GaN epitaxial layers were grown on Si （111） substrates by metal organic chemical vapor deposition. Carbon concentrations in the films grown in different ambients were measured by secondary ion mass spectrometry. The results show that the carbon incorporation is strongly dependent on the H2 flow rate when the NH3 flow rate is small, but insensitive to the H2 flow rate when the NH3 flow rate is sufficient large. We conclude that H2 can inhibit the dissociation of NH3 and result in a less active N source; an insufficiently active N source causes more N vacancies, which enhances carbon incorporation.

关键词： Gallium nitride

Involvement of mind the gap in the organization of the tracheal apical extracellular matrix in Drosophila and Nilaparvata lugens

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2020年第4期27卷 756-770页

作者： Xiang-Zhao Yue Dan Li Jun Lv Kai Liu Jie Chen wen-qing zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen UniversityGuangzhouChina

The tracheal apical extracellular matrix(aECM)is vital for expansion of the tracheal lumen and supports the normal structure of the lumen to guarantee air entry and circulation in *** it has been found that some cuticular proteins are involved in the organization of the aECM,unidentified factors still ***,we found that mind the gap(Mtg),a predicted chitin-binding protein,is required for the normal formation of the apical chitin matrix of airway tubes in the model holometabolous insect Drosophila *** to chitin,the Mtg protein was linearly arranged in the tracheal dorsal trunk of the tracheae in *** mtg expression in the tracheae seriously affected the viability of larvae and caused tracheal chitin spiral defects in some *** of mtg mutant showed that mtg was required for normal development of tracheae in *** taenidial folds of some mtg mutant embryos were found on either lateral view of tracheal dorsal trunk or internal view of transmission electron microscopy *** abnormal tracheae were not fully filled with gas and accompanied by a reduction in tracheal width,which are characteristic phenotypes of tracheal aECM ***,in the hemimetabolous brown planthopper(BPH)Nilapat'vata lugens,downregulation of NICPAP1-N(a homolog of mtg)also led to the formation of abnormal tracheal chitin spirals and *** results suggest that mtg and its homolog are involved in the proper organization of the tracheal aECMs in flies and BPH,and that this function may be conserved in insects.

关键词： chitin Drosophila melanogaster,extracellular matrix mind the gap Nilapm vata lugens trachea

Design parameter optimization method for a prestressed steel structure driven by multi-factor coupling

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Structural and Civil Engineering 2024年第7期18卷 1066-1083页

作者： Guo-Liang SHI Zhan-Sheng LIU De-Chun LU qing-wen zhang Majid DEZHKAM Ze-Qiang WANG Faculty of Architecture Civil and Transportation EngineeringBeijing University of TechnologyBeijing 100124China The Key Laboratory of Urban Security and Disaster Engineering of the Ministry of Education Beijing University of TechnologyBeijing 100124China Beijing Building Construction Research Institute Co. Ltd.Beijing 100039China

To achieve efficient structural design,it is crucial to reduce the cost of materials while ensuring structural *** study proposes an optimization method for design parameters(DPs)in a prestressed steel structure driven by multi-factor *** accomplish this,a numerical proxy model of prestressed steel structures is established with integration of DPs and mechanical parameters(MPs).A data association-parameter analysis-optimization selection system is established.A correlation between DPs and MPs is established using a back propagation(BP)neural *** correlation provides samples for parameter analysis and optimization *** are used to characterize the safety of the *** on the safety grade analysis,the key DPs that affect the mechanical properties of the structure are obtained.A mapping function is created to match the MPs and the key *** optimal structural DPs are obtained by setting structural materials as the optimization objective and safety energy as the constraint *** theoretical model is applied to an 80-m-span gymnasium and verified with a scale test physical *** MPs obtained using the proposed method are in good agreement with the experimental *** with the traditional design method,the design cycle can be shortened by more than 90%.Driven by the optimal selection method,a saving of more than 20% can be achieved through reduction of structural material ***,the cross-sectional dimensions of radial cables have a substantial influence on vertical *** initial tension and cross-sectional size of the upper radial cable exhibit the most pronounced impact on the stress distribution in that *** initial tension and cross-sectional size of the lower radial cable hold the greatest sway over the stress distribution in that cable.

关键词： structure design association relationship performance analysis optimum selection experimental verification

Antiviral Activity of Recombinant Cyanovirin-N against HSV-1

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2010年第6期25卷 432-439页

作者： Hong YU Zong-tao LIU Rui LV wen-qing zhang Department of Medical Microbiology Medical College Qingdao University Qingdao 266071 China Rizhao Municipal Center for Disease Control and Prevention Rizhao 276826 China

In this study,a standard strain of HSV-1 (strain SM44) was used to investigate the antiviral activity of the recombinant Cyanovirin-N (CV-N) against Herpes simplex virus type 1 (HSV-1) in vitro and in *** effect (CPE) and MTT assays were used to evaluate the effect of CV-N on HSV-1 in Vero *** number of copies of HSV-DNA was detected by real-time fluorescence quantitative PCR (FQ-PCR).The results showed that CV-N had a low cytotoxicity on Vero cells with a CC50 of 359.03±0.56 μg/mL,and that it could not directly inactivate HSV-1 ***-N not only reduced the CPE of HSV-1 when added before or after viral infection,with a 50% inhibitory concentration (IC50) with 2.26 and 30.16μg/mL respectively,but it also decreased the copies of HSV-1 DNA in infected host *** encephalitis model for HSV-1 infection was conducted in Kunming mice,and treated with three dosages of CV-N (0.5,5 & 10 mg/kg) which was administered intraperitoneally at 2h,3d,5d,7d post *** duration for the appearance of symptoms of encephalitis and the survival days were recorded and brain tissue samples were obtained for pathological examination (HE staining).Compared with the untreated control group,in the 5mg/kg CV-N and 10mg/kg CV-N treated groups,the mice suffered light symptoms and the number of survival days were more than 9d and 14d *** staining also showed that in 5mg/kg CV-N and 10mg/kg CV-N treated groups,the brain cells did not show visible changes,except for a slight *** results demonstrated that CV-N has pronounced antiviral activity against HSV-1 both in vitro and in vivo,and it would be a promising new candidate for anti-HSV therapeutics.

关键词： Recombinant cyanovirin-N Herpes simplex virus type 1(HSV-1) Antiviral activity Real-time FQ-PCR Encephalitis