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DNA barcoding and molecular phylogeny of Dumasia(Fabaceae:Phaseoleae)reveals a cryptic lineage

Plant Diversity 2020年第5期42卷 376-385页

作者： kai-wen jiang Rong Zhang Zhong-Fu Zhang Bo Pan Bin Tian Key Laboratory of Biodiversity Conservation in Southwest China National Forestry and Grassland AdministrationSouthwest Forestry UniversityKunming650224China Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunming650201YunnanChina University of Chinese Academy of Sciences Beijing100049China Department of Wetland Southwest Forestry UniversityKunming650224China Center for Integrative Conservation Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla666303China Center of Conservation Biology Core Botanical GardensChinese Academy of SciencesMengla666303China Department of Botany and Biodiversity Research University of ViennaRennweg 14A-1030ViennaAustria

Dumasia taxonomy and classification have long been *** within this genus have few morphological differences and plants without flowers or fruits are difficult to accurately *** this study,we evaluated the ability of six DNA barcoding sequences,one nuclear(ITS)and five chloroplast regions(trnH-psbA,matK,rbcL,trnL-trnF,psbB-psbF),to efficiently identify Dumasia *** single markers or their combinations identify obvious barcoding gaps between intraspecific and interspecific genetic *** combined analyses including ITS showed good species resolution and identification *** therefore suggest that ITS alone or a combination of ITS with any cpDNA marker are most suitable for DNA barcoding of *** phylogenetic analyses clearly indicated that Dumasia yunnanensis is not monophyletic and is separated as two independent branches,which may result from cryptic *** results demonstrate that molecular data can deepen the comprehension of taxonomy of Dumasia and provide an efficient approach for identification of the species.

关键词： Cryptic species DNA barcoding Dumasia Internal transcribed spacer(ITS) Plastid genome

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Endodontic management of a fused left maxillary second molar and two paramolars using cone beam computed tomography: A case report

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World Journal of Clinical Cases 2022年第23期10卷 8367-8374页

作者： Xiao-Han Mei Jin Liu Wei Wang Qian-Xia Zhang Tao Hong Shi-Zhu Bai Xiao-Gang Cheng Yu Tian wen-kai jiang Department of Operative Dentistry and Endodontics School of StomatologyThe Fourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina Department of Stomatology Huangshan City People's HospitalHuangshan 245000Anhui ProvinceChina Digital Dentistry Center School of StomatologyThe Fourth Military Medical UniversityXi'an 710032Shaanxi ProvinceChina

BACKGROUND Fused teeth usually involve several complications,such as the development of caries in the groove between fused crowns,tooth impaction,diastemas,aesthetic and periodontal problems,and pulpal pathosis,due to the complex anatomical structure of fused teeth.A thorough diagnosis is paramount to forming an accurate treatment plan and obtaining a favourable *** the advent of cone-beam computed tomography(CBCT),accurate 3-dimensional images of teeth and their surrounding dentoalveolar structures can now be readily obtained,and the technology can accurately provide a minimally invasive approach to acquire detailed diagnostic ***,we utilize CBCT data herein to generate a digital model for the infected region in a patient,and this model enables us to better plan the management of his *** SUMMARY This report details the diagnosis and endodontic treatment of a rare case involving a fused maxillary second molar and two paramolars with apical *** patient experienced pain upon biting and cold sensitivity in the area of the maxillary left *** caries or other defects were identified in these teeth,and a normal response to a pulp electric viability test was *** the aid of CBCT and digital model technology,we initially suspected that the infection originated from the isthmus between the maxillary second molar and two ***,we only treated the isthmus by an endodontic approach and did not destroy the original tooth structure;furthermore,the vital pulp was retained,and good treatment outcomes were observed at the 24-month *** This finding may provide new insights and perspectives on the diagnosis and treatment of fused teeth.

关键词： Cone-beam computed tomography scans Endodontic therapy Fusion of teeth Maxillary molar Digital model technology Case report

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Nuclear phylotranscriptomics and phylogenomics support numerous polyploidization events and hypotheses for the evolution of rhizobial nitrogenfixing symbiosis in Fabaceae

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Molecular Plant 2021年第5期14卷 748-773页

作者： Yiyong Zhao Rong Zhang kai-wen jiang Ji Qi Yi Hu Jing Guo Renbin Zhu Taikui Zhang Ashley N.Egan Ting-Shuang Yi Chien-Hsun Huang Hong Ma State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development Ministry of Education Key Laboratory of Biodiversity and Ecological EngineeringInstitute of Plant BiologyCenter of Evolutionary BiologySchool of Life SciencesFudan University2005 Songhu RoadShanghai 200433China Department of Biology The Huck Institutes of the Life SciencesThe Pennsylvania State UniversityUniversity ParkPA 16802USA Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesLanhei RoadKunming 650201China Key Laboratory of Biodiversity Conservation in Southwest China State Forestry AdministrationSouthwest Forestry UniversityKunming 650224PR China Ningbo Botanical Garden Herbarium Ningbo 315201PR China Xishuangbanna Tropical Botanical Garden Chinese Academy of SciencesMenglunYunnan 666303PR China Department of Biology Utah Valley UniversityOremUT 84058USA

Fabaceae are the third largest angiosperm family,with 765 genera and~19500 *** are important both economically and ecologically,and global Fabaceae crops are intensively studied in part for their nitrogen-fixing ***,resolution of the intrasubfamilial Fabaceae phylogeny and divergence times has remained elusive,precluding a reconstruction of the evolutionary history of symbiotic nitrogen fixation in ***,we report a highly resolved phylogeny using>1500 nuclear genes from newly sequenced transcriptomes and genomes of 391 species,along with other datasets,for a total of 463 legumes spanning all 6 subfamilies and 333 of 765 *** subfamilies are maximally supported as *** clade comprising subfamilies Cercidoideae and Detarioideae is sister to the remaining legumes,and Duparquetioideae and Dialioideae are successive sisters to the clade of Papilionoideae and *** clock estimation revealed an early radiation of subfamilies near the K/Pg boundary,marked by mass extinction,and subsequent divergence of most tribe-level clades within~15 million *** analyses of thousands of gene families support 28 proposed putative whole-genome duplication/whole-genome triplication events across Fabaceae,including those at the ancestors of Fabaceae and five of the subfamilies,and further analyses supported the Fabaceae ancestral *** evolution of rhizobial nitrogen-fixing nodulation in Fabaceae was probed by ancestral character reconstruction and phylogenetic analyses of related gene families and the results support the hypotheses of one or two switches)to rhizobial nodulation followed by multiple ***,these results provide a foundation for further morphological and functional evolutionary analyses across Fabaceae.

关键词： Fabaceae Leguminosae nuclear phylogeny divergence times whole-genome duplication rhizobial nodulation

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A chromosome-scale genome sequence of pitaya (Hylocereus undatus) provides novel insights into the genome evolution and regulation of betalain biosynthesis

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Horticulture Research 2021年第1期8卷 2260-2274页

作者： Jian-ye Chen Fang-fang Xie Yan-ze Cui Can-bin Chen Wang-jin Lu Xiao-di Hu Qing-zhu Hua Jing Zhao Zhi-jiang Wu Dan Gao Zhi-ke Zhang wen-kai jiang Qing-ming Sun Gui-bing Hu Yong-hua Qin State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural Affairs/Lingnan Guangdong Laboratory of Modern AgricultureCollege of HorticultureSouth China Agricultural University510642 GuangzhouGuangdongChina Novogene Bioinformatics Institute 100083 BeijingChina Horticulture Research Institute Guangxi Academy of Agricultural Sciences530007 NanningGuangxiChina Institute of Fruit Tree Research Guangdong Academy of Agricultural Sciences/Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization(MOA)/Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research510640 GuangzhouChina

Pitaya(Hylocereus)is the most economically important fleshy-fruited tree of the Cactaceae family that is grown worldwide,and it has attracted significant attention because of its betalain-abundant ***,the lack of a pitaya reference genome significantly hinders studies focused on its evolution,as well as the potential for genetic improvement of this ***,we employed various sequencing approaches,namely,PacBio-SMRT,Illumina HiSeq paired-end,10×Genomics,and Hi-C(high-throughput chromosome conformation capture)to provide a chromosome-level genomic assembly of‘GHB’pitaya(***,2n=2x=22 chromosomes).The size of the assembled pitaya genome was 1.41 Gb,with a scaffold N50 of~127.15 *** total,27,753 protein-coding genes and 896.31Mb of repetitive sequences in the *** genome were *** has undergone a WGT(whole-genome triplication),and a recent WGD(whole-genome duplication)occurred after the gamma event,which is common to the other species in Cactaceae.A total of 29,328 intact LTR-RTs(~696.45Mb)were obtained in ***,of which two significantly expanded lineages,Ty1/copia and Ty3/gypsy,were the main drivers of the expanded genome.A high-density genetic map of F1 hybrid populations of‘GHB’×‘Dahong’pitayas(***)and their parents were constructed,and a total of 20,872 bin markers were identified(56,380 SNPs)for 11 linkage *** importantly,through transcriptomic and WGCNA(weighted gene coexpression network analysis),a global view of the gene regulatory network,including structural genes and the transcription factors involved in pitaya fruit betalain biosynthesis,was *** data present a valuable resource for facilitating molecular breeding programs of pitaya and shed novel light on its genomic evolution,as well as the modulation of betalain biosynthesis in edible fruits.

关键词： evolution expanded linkage

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The Chromosome-Level Reference Genome of Tea Tree Unveils Recent Bursts of Nonautonomous LTR Retrotransposons in Driving Genome Size Evolution

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Molecular Plant 2020年第7期13卷 935-938页

作者： Qun-Jie Zhang Wei L i Kui Li Hong Nan Cong Shi Yun Zhang Zhang-Yan Dai Yang-Lei Lin Xiao-Lan Yang Yan Tong Dan Zhang Cui Lu Li-Ying Feng Chen-Feng Wang Xiao-Xin Liu Jian-An Huang wen-kai jiang Xing-Hua Wang Xing-Cai Zhang Evan E.Eichler Zhong-Hua Liu and Li-Zhi Gao Institution of Genomics and Bioinformatics South China Agricultural UniversityGuangzhou 510642China Novogene Bioinformatics Institute Building 301Zone A10 Jiuxianqiao North RoadChaoyang DistrictBeijing 100083China School of Life Sciences Nanjing UniversityNanjing 210023China Plant Germplasm and Genomics Center Kunming Institute of BotanyChinese Academy of SciencesKunming 650204China University of the Chinese Academy of Sciences Beijing 100039China Agro-biological Gene Research Center Guangdong Academy of Agricultural SciencesGuangzhou510640China Hunan Agricultural University Changsha 410128China Yunnan Pu’er Tea Tree Breeding Station No.212 Zhenxing AvenueSimao DistrictPu ErYunnan 665099China John A.Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA Howard Hughes Medical Institute University of WashingtonSeattleWA 98195USA

Dear Editor,The tea tree Camellia sinensis,a member of the genus Camellia in the Theaceae family,includes two major cultivated varieties,*** ***(CSA\Assam type)and *** ***(CSS;Chinese type)(Ming and Bartholomew,2007).Due to the high economic importance of the tea tree,considerable efforts have been made to explore genetic basis of the biosynthesis of natural metabolites that determine health benefits and diverse tea flavors(Shi et al.,2011;Li et al.,2011;Li et al.,2015;Xia et aL,2017;Liu et al.,2019).

关键词： Evolution Tree Reference

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Impacts of the annual cycle of background SST in the tropical Pacific on the phase and amplitude of ENSO

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 12-17页

作者： Song jiang Congwen Zhu Ning jiang State Key Laboratory of Severe Weather(LASW) Chinese Academy of Meteorological SciencesBeijingChina College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijingChina

The dominant annual cycle of sea surface temperature(SST)in the tropical Pacific exhibits an antisymmetric mode,which explains 83.4%total variance,and serves as a background of El Niño-Southern Oscillation(ENSO).However,there is no consensus yet on its anomalous impacts on the phase and amplitude of *** on data during 1982-2022,results show that anomalies of the antisymmetric mode can affect the evolution of ENSO on the interannual scale via Bjerknes feedback,in which the positive(negative)phase of the antisymmetric mode can strengthen El Niño(La Niña)in boreal winter via an earlier(delayed)seasonal cycle transition and larger(smaller)annual *** magnitude of the SST anomalies in the equatorial eastern Pacific can reach more than±0.3◦C,regulated by the changes in the antisymmetric mode based on random sensitivity *** reveal the spatial pattern of the annual cycle associated with the seasonal phase-locking of ENSO evolution and provide new insight into the impact of the annual cycle of background SST on ENSO,which possibly carries important implications for forecasting ENSO.

关键词： Annual cycle SST anomaly Antisymmetric mode ENSO

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Cohort study on the treatment of BRAF V600E mutant metastatic colorectal cancer with integrated Chinese and western medicine

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World Journal of Clinical Oncology 2025年第1期16卷 25-33页

作者： jiang-Yu Bian Yu-Fang Feng wen-Ting He Tong Zhang Department of Oncology Xiyuan Hospital of China Academy of Chinese Medical SciencesBeijing 100091China Department of Oncology The Fourth Clinical Medical College of Xinjiang Medical UniversityUrumqi 830001China Department of Oncology Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical UniversityUrumqi 830001China

BACKGROUND Patients with BRAF V600E mutant metastatic colorectal cancer(mCRC)have a low incidence rate,poor biological activity,suboptimal response to conventional treatments,and a poor *** the previous cohort study on mCRC conducted by our team,it was observed that integrated Chinese and Western medicine treatment could significantly prolong the overall survival(OS)of patients with colorectal ***,we further explored the survival benefits in the population with BRAF V600E mutant *** To evaluate the efficacy of integrated Chinese and Western medicine in the treatment of BRAF V600E mutant metastatic colorectal *** A cohort study was conducted on patients with BRAF V600E mutant metastatic colorectal cancer admitted to Xiyuan Hospital of China Academy of Chinese Medical Sciences and Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region from January 2016 to December *** patients were divided into two *** A total of 34 cases were included,with 23 in Chinese-Western medicine cohort(cohort A)and 11 in Western medicine cohort(cohort B).The median overall survival was 19.9 months in cohort A and 14.2 months in cohort B,with a statistically significant difference(P=0.038,hazard ratio=0.46).The 1-3-year survival rates were 95.65%(22/23),39.13%(9/23),and 26.09%(6/23)in cohort A,and 63.64%(7/11),18.18%(2/11),and 9.09%(1/11)in cohort B,*** analysis showed statistically significant differences in median OS between the two cohorts in the right colon,liver metastasis,chemotherapy,and first-line treatment subgroups(P<0.05).CONCLUSION Integrated Chinese and Western medicine can prolong the survival and reduce the risk of death in patients with BRAF V600E mutant metastatic colorectal cancer,with more pronounced benefits observed in patients with right colon involvement,liver metastasis,combined chemotherapy,and first-line treatment.

关键词： Metastatic colorectal cancer BRAF V600E mutation Integrated Chinese and Western medicine Cohort study

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替加环素联合治疗泛耐药肺炎克雷伯菌菌血症病例分析

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中国临床药理学杂志 2017年第8期33卷 742-744页

作者：郑敬苑鑫刘永辉牛文凯李逢将柏长青军事医学科学院附属医院呼吸与危重症医学科北京100071

目的探讨替加环素治疗泛耐药肺炎克雷伯菌所致菌血症的临床疗效。方法对1例使用替加环素治疗泛耐药肺炎克雷伯菌菌血症的病例进行分析。结果和结论替加环素是联合治疗泛耐药肺炎克雷伯菌菌血症的一种选择。

关键词：替加环素泛耐药肺炎克雷伯菌菌血症

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Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He Li Li Sai Wang Shao-jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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Applying blockchain-based method to smart contract classification for CPS applications

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Digital Communications and Networks 2022年第6期8卷 964-975页

作者： Zigui jiang kai Chen Hailin wen Zibin Zheng School of Software Engineering Sun Yat-Sen UniversityChina School of Mathematics Sun Yat-Sen UniversityChina School of Computer Science and Engineering Sun Yat-Sen UniversityChina

Smart contract has been the core of blockchain systems and other blockchain-based systems since Blockchain *** operations on blockchain are performed through the invocation and execution of smart *** leads to extensive combinations between blockchain,smart contract,Internet of Things(IoT)and Cyber-Physical System(CPS)applications,and then many blockchain-based IoT or CPS applications emerge to provide multiple benefits to the economy and *** this case,obtaining a better understanding of smart contracts will contribute to the easier operation,higher efficiency and stronger security of those blockchain-based systems and *** existing studies on smart contract analysis are based on similarity calculation and smart contract ***,smart contract is a piece of code with special characteristics and most of smart contracts are stored without any category labels,which leads to difficulties of smart contract *** the back end of a blockchain-based Decentralized Application(DApp)is one or several smart contracts,DApps with labeled categories and open source codes are applied to achieve a supervised smart contract classification.A three-phase approach is proposed to categorize DApps based on various data *** this approach,5,659 DApps with smart contract source codes and pre-tagged categories are first obtained based on massive collected DApps and smart contracts from Ethereum,State of the DApps and *** feature extraction and construction methods are designed to form multi-feature vectors that could present the major characteristics of ***,a fused classification model consisting of KNN,XGBoost and random forests is applied to the multi-feature vectors of all DApps for performing DApp *** experimental results show that the method is *** addition,some positive correlations between feature variables and categories,as well as several user behavior patterns of DAp

关键词： Blockchain Blockchain-based application Smart contract DApP classification Solidity

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