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Journal of Integrative Agriculture 2017年第6期16卷 1358-1366页

作者： LIU Niu CHE jun LAI Duo wen jin-jun XU Han-hong State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources & Key Laboratory of Natural Pesticide and Chemical Biology Ministry of Education/South China Agricultural University Guangzhou 510642 P.R.China Shenzhen Longgang Inspection Station of Non-pollution Agricultural Products Shenzhen 510172 P.R. China Institute of Fruit Tree Research Guangdong Academy of Agricultural Sciences Guangzhou 510640 P.R. China

The acetylcholinesterase 2（AChE2）cloned from Plutella xylostella was first successfully expressed in methylotrophic yeast Pichia pastoris *** transformant with high-level expression of the recombinant AChE（rAChE,23.2 U mL-1in supernatant）was selected by plating on increasing concentrations of antibiotic G418 and by using a simple and specific chromogenic reaction with indoxyl acetate as a *** maximum production of r ACh E reached about 11.8 mg of the enzyme protein per liter of *** r ACh E was first precipitated with ammonium sulfate（50%saturation）and then purified with procainamide affinity column *** enzyme was purified 12.1-fold with a yield of 22.8%and a high specific activity of 448.3 U *** was sensitive to inhibition by methamidophos and pirimicarb,the calculated 50% inhibitory concentration（IC50）values of the two pesticides were 0.357 and 0.888 mg L-1,respectively,and the calculated 70% inhibitory concentration（IC70）values were 0.521 and 0.839 mg L-1,*** results suggested that it has a potential application in the detection of pesticide residues.

关键词： Plutella xylostella acetylcholinesterase Pichia pastoris high-level expression

Diagnostic value of interleukin-8 in colorectal cancer:A case-control study and meta-analysis

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World Journal of Gastroenterology 2014年第43期20卷 16334-16342页

作者： wen-jun jin jin-Ming Xu wen-Li Xu Dong-Hua Gu Pei-Wei Li Department of Clinical Laboratory Huzhou Central Hospital Department of Epidemiology and Health Statistics Zhejiang University School of Public Health Department of Pathology Huzhou Central Hospital

AIM: To assess the diagnostic value of serum interleukin-8 (IL-8) in the detection of colorectal cancer (CRC).

关键词： Interleukin-8 Colorectal cancer Diagnostic value Case-control study Meta-analysis

Directed Molecular Evolution of Nitrite Oxido-reductase by DNA-shuffling

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Biomedical and Environmental Sciences 2007年第2期20卷 113-118页

作者： jun-wen LI jin-LAI ZHENG XIN-WEI WANG MIN jin FU-HUAN CHAO Institute of Environment and Health 1 Dali Road Tianjin 300050 China

Objective To devellop directly molecular evolution Of nitrite oxido-reductase using DNA-shuffling technique because nitrobacteria grow extremelly slow and are unable to nitrify effectively inorganic nitrogen in wastewater treatmem. Methods The norB gene coding the ntitrite oxido-reductase in nitrobacteria was cloned and sequenced. Then, directed molecular evolution of nitrite oxido-reductase was developed by DNA-shuffling of 15 norB genes from different nitrobacteria. Results After DNA-shuffling with sexual PeR and staggered extension process PCR, the sequence was differem from its parental DNA fragmems and the homology ranged from 98% to 99%. The maximum nitrification rate of the modified bacterium of X16 by DNA-shuffling was up to 42.9 mg/L.d, which was almost 10 times higher than that of its parental bacteria. Furthermore, the modified bacterium had the same characteristics of its parental bacteria of E. coli and could grow rapidly in normal cultures. Conclusion DNA-shuffling was successfully used to engineer E. coli, which had norB gene and could degrade inorganic nitrogen effectively.

关键词： Directed evolution DNA-shuffling Nitrite oxido-reductase norB gene Nitrobacteria

High mobility group box 1 in the central nervous system:regeneration hidden beneath inflammation

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Neural Regeneration Research 2025年第1期20卷 107-115页

作者： Hanki Kim Bum jun Kim Seungyon Koh Hyo jin Cho Xuelian jin Byung Gon Kim jun Young Choi Department of Brain Science Ajou University School of MedicineSuwonSouth Korea Department of Biomedical Sciences Ajou University Graduate School of MedicineSuwonSouth Korea Department of Neurology Ajou University School of MedicineSuwonSouth Korea Department of Geriatrics The Affiliated Suqian First People’s Hospital of Nanjing Medical UniversitySuqianJiangsu ProvinceChina

High-mobility group box 1 was first discovered in the calf thymus as a DNA-binding nuclear protein and has been widely studied in diverse fields,including neurology and ***-mobility group box 1 in the extracellular space functions as a pro-inflammatory damage-associated molecular pattern,which has been proven to play an important role in a wide variety of central nervous system disorders such as ischemic stroke,Alzheimer’s disease,frontotemporal dementia,Parkinson’s disease,multiple sclerosis,epilepsy,and traumatic brain *** drugs that inhibit high-mobility group box 1 as a damage-associated molecular pattern,such as glycyrrhizin,ethyl pyruvate,and neutralizing anti-high-mobility group box 1 antibodies,are commonly used to target high-mobility group box 1 activity in central nervous system *** it is commonly known for its detrimental inflammatory effect,high-mobility group box 1 has also been shown to have beneficial pro-regenerative roles in central nervous system *** this narrative review,we provide a brief summary of the history of high-mobility group box 1 research and its characterization as a damage-associated molecular pattern,its downstream receptors,and intracellular signaling pathways,how high-mobility group box 1 exerts the repair-favoring roles in general and in the central nervous system,and clues on how to differentiate the pro-regenerative from the pro-inflammatory *** targeting high-mobility group box 1 in the central nervous system may benefit from differentiating between the two functions rather than overall suppression of high-mobility group box 1.

关键词： central nervous system damage-associated molecular pattern ethyl pyruvate glycyrhizzin high mobility group box 1 inflammation neural stem cells neurodevelopment oligodendrocyte progenitor cells redox status regeneration

Activin A receptor type 1C single nucleotide polymorphisms associated with esophageal squamous cell carcinoma risk in Chinese population

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 39-51页

作者： Si-Yun Lin Hou Huang jin-Jie Yu Feng Su Tian Jiang Shao-Yuan Zhang Lu Lv Tao Long Hui-wen Pan jun-Qing Qi Qiang Zhou Wei-Feng Tang Guo-wen Ding Li-Ming Wang Li-Jie Tan jun Yin Department of Thoracic Surgery Zhongshan Hospital Affiliated to Fudan UniversityShanghai 200032China Shanghai Key Laboratory of Clinical Geriatric Medicine Huadong Hospital Affiliated to Fudan UniversityShanghai 200040China Department of Cardiothoracic Surgery The Affiliated People's Hospital of Jiangsu UniversityZhenjiang 212002Jiangsu ProvinceChina Department of Thoracic Surgery Sichuan Cancer Hospital&InstituteChengdu 610042Sichuan ProvinceChina Department of Cardiothoracic Surgery Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210000Jiangsu ProvinceChina Department of Respiratory and Critical Care Medicine Shanghai Xuhui Central HospitalShanghai 200032China

BACKGROUND Transforming growth factor-β(TGF-β)superfamily plays an important role in tumor progression and *** A receptor type 1C(ACVR1C)is a TGF-βtype I receptor that is involved in tumorigenesis through binding to dif-ferent *** To evaluate the correlation between single nucleotide polymorphisms(SNPs)of ACVR1C and susceptibility to esophageal squamous cell carcinoma(ESCC)in Chinese Han *** In this hospital-based cohort study,1043 ESCC patients and 1143 healthy controls were *** SNPs(rs4664229,rs4556933,rs77886248,rs77263459,rs6734630)of ACVR1C were assessed by the ligation detection reaction ***-Weinberg equilibrium test,genetic model analysis,stratified analysis,linkage disequi-librium test,and haplotype analysis were *** Participants carrying ACVR1C rs4556933 GA mutant had significantly decreased risk of ESCC,and those with rs77886248 TA mutant were related with higher risk,especially in older male *** the haplotype analysis,ACVR1C Trs4664229Ars4556933Trs77886248Crs77263459Ars6734630 increased risk of ESCC,while Trs4664229Grs4556933Trs77886248Crs77263459Ars6734630 was associated with lower susceptibility to *** ACVR1C rs4556933 and rs77886248 SNPs were associated with the susceptibility to ESCC,which could provide a potential target for early diagnosis and treatment of ESCC in Chinese Han population.

关键词： Activin A receptor type 1C Single nucleotide polymorphisms Esophageal squamous cell carcinoma Genetic susceptibility Hospital-based cohort study

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论邓小平后勤思想的形成历程

长沙理工大学学报（社会科学版） 2007年第1期22卷 9-11页

作者：文进军湖南科技大学法学院湖南湘潭411201

邓小平后勤思想作为一个理论体系,不仅具有丰富的内涵,且有一个明晰的形成历程,邓小平在汲取古代后勤思想和毛泽东后勤思想的基础上,对后勤工作的地位和作用有自己独特的理解和认识,为后勤工作提供了不竭的理论源泉。

关键词：邓小平后勤思想思维历程

急诊内镜套扎联合奥曲肽治疗肝硬化合并食管静脉曲张出血的疗效分析

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医学临床研究 2012年第5期29卷 938-940页

作者：文进军湖南省衡阳市第一人民医院消化科湖南衡阳421001

[目的]探讨急诊内镜套扎联合奥曲肽治疗肝硬化合并食管静脉曲张出血的疗效.[方法]将121例肝硬化合并食管静脉曲张出血患者分为观察组（n=61）与对照组（n=60）,观察组患者接受急诊内镜套扎联合奥曲肽治疗,对照组患者接受奥曲肽治疗.[结... 详细信息

[目的]探讨急诊内镜套扎联合奥曲肽治疗肝硬化合并食管静脉曲张出血的疗效.[方法]将121例肝硬化合并食管静脉曲张出血患者分为观察组（n=61）与对照组（n=60）,观察组患者接受急诊内镜套扎联合奥曲肽治疗,对照组患者接受奥曲肽治疗.[结果]①观察组止血成功率显著高于对照组（P＜0.05）,早期再出血发生率显著低于对照组（P＜0.05）;两组患者病死率相比差异无统计学意义（P＞0.05）.②观察组止血时间、奥曲肽治疗天数、输血量、住院时间显著少于对照组（均P＜0.05）.③观察组不良反应发生率显著高于对照组（P＜0.05）.[结论]急诊内镜套扎联合奥曲肽治疗肝硬化合并食管静脉曲张出血的患者的效果显著.

关键词：肝硬化/并发症食管和胃静脉曲张/并发症胃肠出血/治疗外科手术,内窥镜奥曲肽/治疗应用

Curcumin in gastric cancer treatment:A commentary on mechanistic insights and future directions

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 265-267页

作者： Xin-Yue Wei wen-Bo Cao Sai-jun Mo Zhi-Yan Sun Department of Pathophysiology School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450001Henan ProvinceChina School of Basic Medical Science Zhengzhou UniversityZhengzhou 450001Henan ProvinceChina Department of Basic Science of Oncology School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450001Henan ProvinceChina Department of Special Service No.988 Hospital of the Joint Service Support Force of PLAZhengzhou 450042Henan ProvinceChina

The study by Yang et al presents a comprehensive investigation into the thera-peutic potential of curcumin for gastric cancer(GC).Using network pharma-cology,the researchers identified 48 curcumin-related genes,31 of which overlap with GC *** genes,including ESR1,EGFR,CYP3A4,MAPK14,CYP1A2,and CYP2B6,are linked to poor survival in GC *** docking con-firmed strong binding affinity of curcumin to these *** vitro experiments demonstrated that curcumin effectively inhibits the growth and proliferation of BGC-823,suggesting its therapeutic potential in GC through multiple targets and pathways.

关键词： Curcumin Gastric cancer Network pharmacology Mechanism of action In vitro experiments

Study on Heterosis and Parent-offspring Correlation between Supper Fibre Varieties and Insect-resistant Transgenic Varieties in Upland Cotton

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Study on Heterosis and Parent-offspring Correlation between ...

腹部常用穴穴区微循环血流灌注量的差异及不同温度艾灸的影响

湖北省遗传学会、江西省遗传学会2006年学术年会暨学术讨论会

作者： TANG wen-wu, XIAO wen-jun, HUANG Ying-jin, WU Xiu-lan ( College of Agronomy, Jiangxi Agricultural University, Nanchang 330045 Dept of biology, Zhaoqing University, Zhaoqing 526061)

62 cross combinations were made between 32 supper fibre varieties and 8 insect-resistant transgenic varieties, Analyzed the heterosis and parent-offspring correlation. The result showed that F1 hybrids had strongest yield heterosis. The boll weight has the highest heterosis, lint percentage was the second importance, while boll number and seed index has negative heterosis among the yield components. Fiber quality traits in F1 hybrids had low mid-parents heterosis, but had high comparison heterosis. The positive correlation between F1 hybrids and male, female in boll weight and fiber quality characters were significant at 0.05 levels, respectively. Positive correlation between F1 hybrids and male in gross period, lint percentage were significant at 0.05 levels, positive correlation between F1 hybrids and female in No. of fruit branches was significant at 0.05 levels.

关键词： insect-resistant cotton heterosis parent-offspring correlation

来源： cnki会议评论

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南京中医药大学学报 2017年第4期33卷 339-343页

作者：蒋颖戴文君姜劲峰南京中医药大学第二临床医学院江苏南京210023 无锡市中医院针灸科江苏无锡214071

目的观察正常生理状态下腹部常用穴穴区微循环的规律特点,比较不同温度艾灸对其微循环的影响差异。方法纳入52例正常青年志愿者(男18,女34),采用激光多普勒血流仪依次测量其腹部的神阙、肓俞、天枢、大横、水分、气海、关元、中脘穴穴... 详细信息

目的观察正常生理状态下腹部常用穴穴区微循环的规律特点,比较不同温度艾灸对其微循环的影响差异。方法纳入52例正常青年志愿者(男18,女34),采用激光多普勒血流仪依次测量其腹部的神阙、肓俞、天枢、大横、水分、气海、关元、中脘穴穴区皮肤微循环值;随后从中选取神阙、天枢穴进行不同温度(40~43℃和>43℃,<44℃)艾灸干预,连续灸7次,每次2种温度各2min,观察不同温度艾灸对该两穴穴区皮肤微循环的影响。结果 (1)正常生理状态下,神阙穴穴区微循环值明显高于其他所测穴位穴区的微循环值(P<0.01);肓俞穴微循环值高于天枢穴(P<0.05)、大横穴(P<0.01);天枢穴微循环值高于大横穴(P<0.01)。(2)艾灸后神阙、天枢穴微循环值均升高且神阙穴在2种温度艾灸时的微循环均值、最大值、与艾灸前的差值皆大于天枢穴(P<0.01);在艾灸刺激下,神阙穴微循环值较天枢穴先出现变化并且晚进入平台期;神阙穴微循环值对艾灸温度变化的反应比天枢穴敏感。结论腹部常用穴位中,神阙穴穴区的皮肤微循环值最高;腹部水平平脐的穴位整体表现出越接近脐,其皮肤微循环值越高的趋势。同等温灸条件下神阙穴穴区皮肤微循环值的升高速度、幅度和持续升高时间都优于天枢穴。初步明确神阙穴在腹部常用穴位中具有微循环的特殊性与优越性,为穴位微循环结构特异性提供有效的证据支撑。

关键词：神阙穴微循环艾灸