检索结果-南通市图书馆

Epigenetic modification regulates both expression of tumor-associated genes and cell cycle progressing in human colon cancer cell lines: Colo-320 and SW1116

在线全文

学校读者我要写书评

暂无评论

Cell Research 2004年第3期14卷 217-226页

作者： Jing Yuan fang, Ying Xuan CHEN, juan LU, Rong LU, Li YANG, Hong Yin ZHU, Wei Qi GU, Lun Gen LU Shanghai Institute of Digestive Disease, Renji Hospital Shanghai Second Medical University, Shanghai 200001, China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China. 1Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai 200001 China.

The aim of this study is to assess the effects of DNA methylation and historic acetylation, alone or in combination, on the expression of several tumor-associated genes and cell cycle progression in two established human colon cancer cell lines: Colo-320 and SW1116. Treatments with 5-aza-2’-deoxycytidine (5-aza-dC) and trichostatin A, alone or in combination, were applied respectively. The methylation status of the CDKN2A promoter was determined by methyla-tion-specific PCR, and the acetylated status of the histones associated with the p21WAF1 and CDKN2A genes was examined by chromatin immunoprecipitation. The expression of the CDKN2A, p21WAF1, p53, p73, APC, c-myc, c-Ki-ras and survivin genes was detected by real-time RT-PCR and RT-PCR. The cell cycle profile was established by flow cytometry. We found that along with the demethylation of the CDKN2A gene promoter in both cell lines induced by 5-aza-dC alone or in combination with TSA, the expression of both CDKN2A and APC genes increased. The treatment

关键词： human colon cancer cell lines tumor-associated genes DNA methylation histone acetylation cell cycle.

Evolutionary rates of mitochondrial sequences and gene orders in Spirurina(Nematoda)are episodic but synchronised

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Water Biology and Security 2022年第2期1卷 49-59页

作者： Hong Zou Hong-Peng Lei Rong Chen fang-Lin Chen wen-Xiang Li Ming Li Dong Zhang Ivan Jakovlic Gui-Tang Wang Key Laboratory of Aquaculture Disease Control Ministry of AgricultureState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of HydrobiologyChinese Academy of SciencesWuhan 430072China State Key Laboratory of Grassland Agro-Ecosystem Institute of Innovation EcologyLanzhou UniversityLanzhou 730000China Bio-Transduction Lab Wuhan 430075China University of Chinese Academy of Sciences Beijing 100049China

In contrast to highly conserved mitogenomic architecture in most metazoan lineages,which indicates that rearrangement events are generally strongly selected against,a limited number of often unrelated lineages exhibit highly elevated architectural evolution *** underlying reasons for this discontinuity in the mitogenomic evolution remain *** we sequenced the mitochondrial genome of the first Camallanoidea species,Camallanus cotti(Nematoda:Chromadorea:Spirurina:Camallanidae),and found that it exhibited a highly disrupted *** hypothesised that disrupted architecture might be a synapomorphic feature of the sister-clades Camallanoidea and *** this study,we sequenced mitogenomes of three freshwater fish-parasitic nematodes:Camallanus lacustris(Camallanidae),and two Philometridae(Dracunculoidea)species,Clavinema parasiluri,and Philometra *** partial agreement with the working hypothesis,both Camallanoidea species had exceptionally large mitogenomes of 18–19 Kbp,albeit the underlying reasons differed:in *** it was the existence of a single enlarged noncoding region of5.5 Kbp.A segment of this region exhibited an inverted base composition skew,which is indicative of a sequence inversion or recombination *** is the second identified chromadorean(first for Spirurina)family that exhibits within-family protein-coding gene rearrangements,and the absence of trnL1 and trnF may be a synapomorphy for *** underlying reason for the disrupted architecture of Camallanidae does not appear to be a particular event shared by their common ancestor,but rather an underlying mechanism that makes disruptive events more likely in this *** disagreement with the working hypothesis,Spiruromorpha and Oxyuridomorpha exhibited even more highly rearranged gene orders and greater overall branch lengths than ***,withininfraorder architecture was highly conserved and leaf nodes very ***

关键词： Branch length Gene order rearrangement Inversion Recombination Molecular clock Episodic evolution Camallanidae Philometridae Camallanomorpha

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

气泡羽流空隙率的计算及其不稳定规律的研究

水利学报 2011年第4期42卷 419-424页

作者：宋策程文胡保卫程文娟西安理工大学陕西西安710048 西安交通大学多相流国家重点实验室陕西西安710049 绍兴文理学院浙江绍兴312000

在气液两相流中,气相和液相各自有不同的流场,而液相的速度场取决于气泡的运动特性。空隙率是气液两相流中描述气泡分散相间相互作用的一个重要的参数,通过对气泡羽流的空隙率分布分析,可了解气泡羽流的运动机理与实验条件之间的关系。... 详细信息

在气液两相流中,气相和液相各自有不同的流场,而液相的速度场取决于气泡的运动特性。空隙率是气液两相流中描述气泡分散相间相互作用的一个重要的参数,通过对气泡羽流的空隙率分布分析,可了解气泡羽流的运动机理与实验条件之间的关系。本文采用图像处理的方法测量在高密度气泡下的瞬时空隙率分布。对拍摄的图片进行预处理,得到其灰度图像和灰度值,建立其局部灰度值与投影空隙率间的相关关系,进一步计算出气泡羽流的不同位置处的空隙率值分布,在此基础上分析不同工况下空隙率值的分布情况及其对气泡羽流结构的影响。结果表明:该方法适合用于气液两相流空隙率的测量,在纵横比为1.0时,气泡羽流的结构受压强影响小,羽流结构稳定;纵横比为1.5和2.0时,随着压强的增大,羽流结构呈现不稳定。

关键词：气泡羽流空隙率灰度值纵横比

纳米MnO_2的固相合成及其电化学性能的研究(Ⅰ)──纳米γ-MnO_2的合成及表征

在线全文

学校读者我要写书评

暂无评论

高等学校化学学报 1999年第9期20卷 1434-1437页

作者：李娟夏熙李清文新疆大学应用化学研究所乌鲁木齐830046

用醋酸锰分别与柠檬酸、草酸和8-羟基喹啉在室温及低温下发生固相反应生成锰配合物，经热分解和酸处理等手段最终制备出纳米γ－MnO2用元素分析、化学分析、X射线衍射、热重／差热分析和透射电镜等测试手段对固相反应的可行性作了讨论... 详细信息

用醋酸锰分别与柠檬酸、草酸和8-羟基喹啉在室温及低温下发生固相反应生成锰配合物，经热分解和酸处理等手段最终制备出纳米γ－MnO2用元素分析、化学分析、X射线衍射、热重／差热分析和透射电镜等测试手段对固相反应的可行性作了讨论，并对合成的样品进行了表征．从TEM照片上看，产物粒子呈球形，粒径多为20～30nm．酸处理可大大提高样品的氧化度，但酸化过程中粒子出现团聚现象，粒子形貌也有所改变，似有取向生长的趋势．文中还讨论了低温条件下利用固相反应制备纳米材料的原因．

关键词：固相合成纳米材料二氧化锰电极材料

Ni/Mo_2 N as a Highly Active Hydrodenitrogenation Catalyst

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1996年第9期7卷 849-852页

作者： wen Yu ZHANG Xin Ping WANG Heng fang JIN Qin XIN (State key Laboratory of Catalysis,Dalian Institute of Chemical Physiscs,Chinese Academy Of Sciences,Dalian 116023)(Inner Mongolia Polytechnic Universal,Huhhot 010062) Inner Mongolia Polytech. University Huhhot 010062 State Key Laboratory of Catalysis Dalian Inst. of Chemical Physiscs Chinese Academy of Sciences Dalian 116023

The catalysts were prepared by the temperature programmed reaction (TPR) of MoO3 with NH3 at various temperatures in the range of 573K～973K, and their hydrodenitrogenation (HDN) activities were tested in *** is shown that molybdenum nitrid(Mo2N) was formed above 923K and its intermediate MoO2 formed at about 573～623K under the rapid (5K/min.) TPR ***2N is the most active species for pyridine HDN among MoO3,MoO2,MoS2,and Mo2N. Moreover,it can be promoted by adding Ni *** is shown that the Ni/Mo2N catalyst prepared by adding some NiO into the precursor MoO3 has a steady HDN activity Which is far higher than that of the commercial sulfided NiMo/Al2O3(HR346) catalyst.

关键词： Mo Ni/Mo2 N as a Highly Active Hydrodenitrogenation Catalyst

Increased Chondrocyte Apoptosis in Kashin-Beck Disease and Rats Induced by T-2 Toxin and Selenium Deficiency

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2017年第5期30卷 351-362页

作者： YANG Hao Jie ZHANG Ying WANG Zhi Lun XUE Sen Hai LI Si Yuan ZHOU Xiao Rong ZHANG Meng fang Qian WANG wen Jun CHEN Chen DENG Xiang Hua CHEN Jing Hong Institute of Endemic Diseases School of Public HealthXi'an Jiaotong University Health Science CenterKey Laboratory of Trace Elements and Endemic DiseasesNational Health and Family Planning CommissionXi'an 710061ShaanxL China Xi'an Jiaotong University Hospital Xi'an 710049ShaanxL China School of Biomedical Sciences the University of QueenslandBrisbane 4072Australia Laboratory for Soft Tissue Research Hospital for Special SurgeryNew York 10021USA

Objective To investigate chondrocyte apoptosis and the expression of biochemical markers associated with apoptosis in Kashin-Beck disease（KBD） and in an established T-2 toxin-and selenium（Se） deficiency-induced rat model. Methods Cartilages were collected from the hand phalanges of five patients with KBD and five healthy children. Sprague-Dawley rats were administered a selenium-deficient diet for 4 weeks prior to T-2 toxin exposure. The apoptotic chondrocytes were observed by terminal deoxynucleotidyl transferase d UTP nick end labeling staining. Caspase-3, p53, Bcl-2, and Bax proteins in the cartilages were visualized by immunohistochemistry, their protein levels were determined by Western blotting, and m RNA levels were determined by real-time reverse transcription polymerase chain reaction. Results Increased chondrocyte apoptosis was observed in the cartilages of children with KBD. Increased apoptotic and caspase-3-stained cells were observed in the cartilages of rats fed with normal and Se-deficient diets plus T-2 toxin exposure compared to those in rats fed with normal and Se-deficient diets. Caspase-3, p53, and Bax proteins and m RNA levels were higher, whereas Bcl-2 levels were lower in rats fed with normal or Se-deficiency diets supplemented with T-2 toxin than the corresponding levels in rats fed with normal diet. Conclusion T-2 toxin under a selenium-deficient nutritional status induces chondrocyte death, which emphasizes the role of chondrocyte apoptosis in cartilage damage and progression of KBD.

关键词： KBD Chondrocyte Apoptosis T-2 toxin Selenium-deficiency

Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第1期15卷 58-66页

作者： You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU fang LIU Division of Nephrology West China Hospital of Sichuan University Chengdu 610041 China

Our intent is to examine the predictive role of Charlson comorbidity index （CCI） on mortality of patients with type 2 diabetic nephropathy （DN）. Based on the CCI score, the severity of comorbidity was categorized into three grades： mild, with CCI scores of 1-2; moderate, with CCI scores of 3-4; and severe, with CCI scores 〉5. Factors influencing mortality and differences between groups stratified by CCI were determined by logistical regression analysis and one-way analysis of variance （ANOVA）. The impact of CCI on mortality was assessed by the Kaplan- Meier analysis. A total of 533 patients with type 2 DN were enrolled in this study, all of them had comorbidity （CCI score 〉1）, and 44.7% （238/533） died. The mortality increased with CCI scores： 21.0% （50/238） patients with CCI scores of 1-2, 56.7% （135/238） patients with CCI scores of 3-4, and 22.3% （53/238） patients with CCI scores 〉5. Logistical regression analysis showed that CCI scores, hemoglobin, and serum albumin were the potential predictors of mortality （P〈0.05）. One-way ANOVA analysis showed that DN patients with higher CCI scores had lower levels of hemoglobulin, higher levels of serum creatinine, and higher mortality rates than those with lower CCI scores. The Kaplan-Meier curves showed that survival time decreased when the CCI scores and mortality rates went up. In con- clusion, CCI provides a simple, readily applicable, and valid method for classifying comorbidities and predicting the mortality of type 2 DN. An increased awareness of the potential comorbidities in type 2 DN patients may provide insights into this complicated disease and improve the outcomes by identifying and treating patients earlier and more effectively.

关键词： Diabetic nephropathy (DN) Charlson comorbidity index (CCI) Mortality

基于整合药理学分析丁香柿蒂汤降逆止呃的分子机制

在线全文

学校读者我要写书评

暂无评论

中国实验方剂学杂志 2019年第3期25卷 100-107页

作者：丁芳芳彭修娟唐文强张瑞瑞刘峰陕西国际商贸学院陕西咸阳712046 陕西步长制药有限公司西安710075

目的:探讨经典名方丁香柿蒂汤降逆止呃的分子作用机制,为该方的后续研究与开发具有提供参考。方法:利用中药整合药理学平台,从多成分、多靶标的维度揭示丁香柿蒂汤降逆止呃的潜在分子机制。结果:丁香柿蒂汤降逆止呃作用主要与89个化学... 详细信息

目的:探讨经典名方丁香柿蒂汤降逆止呃的分子作用机制,为该方的后续研究与开发具有提供参考。方法:利用中药整合药理学平台,从多成分、多靶标的维度揭示丁香柿蒂汤降逆止呃的潜在分子机制。结果:丁香柿蒂汤降逆止呃作用主要与89个化学成分相关,共有靶标1个[大麻素受体1(CNR1)],CNR1一方面通过抑制γ-氨基丁酸(GABA),多巴胺和5-羟色胺(5-HT)的释放而抑制呃逆的发生;另一方面,CNR1激活可抑制胃肠动力、延迟胃排空,推测可能是通过脑-肠轴发挥调节胃肠运动的作用。同时,丁香柿蒂汤降逆止呃作用可能与神经系统、核苷酸代谢相关。结论:丁香柿蒂汤可能是通过影响神经递质的释放、线粒体能量代谢等调节胃肠运动,基于多靶标、多通路共同干预实现其降逆止呃的作用。

关键词：经典名方丁香柿蒂汤整合药理学呃逆作用机制神经系统核苷酸代谢

氟化物和抗菌材料联合运用在龋病防治中的研究进展

在线全文

学校读者我要写书评

暂无评论

牙体牙髓牙周病学杂志 2024年第3期29卷 177-181页

作者：陈鸿炜袁一方周子超张倩霞蒋文凯王胜朝口颌系统重建与再生全国重点实验室国家口腔疾病临床医学研究中心陕西省口腔医学重点实验室第四军医大学口腔医院牙体牙髓病科联勤保障部队第九二八医院

龋病是在以细菌为主的多种因素影响下，发生在牙体硬组织的一种慢性进行性破坏性疾病。龋病的发生因素和病理结构特点提示我们单独使用抗菌材料或再矿化材料都只能起到有限的龋病防治效果，而将二者联合运用则是一种更为积极有效的龋病... 详细信息

龋病是在以细菌为主的多种因素影响下，发生在牙体硬组织的一种慢性进行性破坏性疾病。龋病的发生因素和病理结构特点提示我们单独使用抗菌材料或再矿化材料都只能起到有限的龋病防治效果，而将二者联合运用则是一种更为积极有效的龋病防治手段。氟化物是最常用的再矿化材料，将其与抗菌材料联合运用，不仅可以减轻过量使用氟化物带来的不良后果，还可以有效阻止龋病的发生发展，是近年来人们研究的热点。本文就氟化物和抗菌材料联合运用在龋病防治中的研究进展作一综述。

关键词：龋病氟化物抗菌材料联合运用