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Hypomethylation of glycine dehydrogenase promoter in peripheral blood mononuclear cells is a new diagnostic marker of hepatitis B virus-associated hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 35-42页

作者： Li-Li Miao jing-wen wang Hui-Hui Liu Shuai Gao Yu-Chen Fan Kai wang Department of Hepatology Qilu Hospital of Shandong UniversityJinan 250012China Experimental Center Shandong University of Traditional Chinese MedicineJinan 250355China Institute of Hepatology Shandong UniversityJinan 250012China

Background: Glycine dehydrogenase(GLDC) plays an important role in the initiation and proliferation of several human cancers. In this study, we aimed to detect the methylation status of GLDC promoter and its diagnostic value for hepatitis B virus-associated hepatocellular carcinoma(HBV-HCC). Methods: We enrolled 197 patients, 111 with HBV-HCC, 51 with chronic hepatitis B(CHB), and 35 healthy controls(HCs). The methylation status of GLDC promoter in peripheral mononuclear cells(PBMCs) was identified by methylation specific polymerase chain reaction(MSP). The mRNA expression was examined using real-time quantitative polymerase chain reaction(q PCR). Results: The methylation frequency of the GLDC promoter was significantly lower in HBV-HCC patients(27.0%) compared to that in CHB patients(68.6%) and HCs(74.3%)( P < 0.001). The methylated group had lower alanine aminotransferase level( P = 0.035) and lower rates of tumor node metastasis(TNM) Ⅲ/Ⅳ( P = 0.043) and T3/T4( P = 0.026). TNM stage was identified to be an independent factor for GLDC promoter methylation. GLDC mRNA levels in CHB patients and HCs were significantly lower than those in HBV-HCC patients( P = 0.022 and P < 0.001, respectively). GLDC mRNA levels were significantly higher in HBV-HCC patients with unmethylated GLDC promoters than those with methylated GLDC promoters( P = 0.003). The diagnostic accuracy of alpha-fetoprotein(AFP) combined with GLDC promoter methylation for HBV-HCC was improved compared with that of AFP alone(AUC: 0.782 vs. 0.630, P < 0.001). In addition, GLDC promoter methylation was an independent predictor for overall survival of HBV-HCC patients( P = 0.038). Conclusions: The methylation frequency of GLDC promoter was lower in PBMCs from HBV-HCC patients than that from patients with CHB and HCs. The combination of AFP and GLDC promoter hypomethylation significantly improved the diagnostic accuracy of HBV-HCC.

关键词： Hepatocellular carcinoma Glycine dehydrogenase DNA methylation Peripheral blood mononuclear cells

Nitro-oleic acid ameliorates erectile dysfunction in a streptozotocin-induced rat model of diabetes by inhibiting oxidative stress and apoptosis and activating the NO/cGMP pathway

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Asian Journal of Andrology 2024年第1期26卷 57-66页

作者： Chang-Bo Zhao Wei-Bo Chen wen-Zhen wang Fang-Xin Gong Cui-Qin Fan Ye Li Tian Lan wen-jing wang Ming-Zhen Yuan Department of Urology Shandong Provincial HospitalShandong UniversityJinan 250012China Department of Andrology Liaocheng People's HospitalShandong UniversityLiaocheng 252000China Department of Urology The Second Hospital of Shandong UniversityJinan 250012China Department of Physiology School of Basic Medical SciencesShandong UniversityJinan 250012China

The major vascular complications associated with diabetes make the management of diabetic mellitus erectile dysfunction(DMED)a challenging *** factors contributing to DMED include oxidative stress,nitric oxide(NO)/cyclic guanosine monophosphate(cGMP)pathway activation,and apoptosis,while nitro-oleic acid(NO,-OA)has been shown to be beneficial in treating these aspects of this ***,herein,investigated the effects and possible mechanisms of NO,-OA on erectile function as assessed in a streptozotocin-induced rat model of *** results revealed that the erectile function of DMED rats was significantly impaired compared with that of the control ***,in response to 4 weeks of NO,-OA treatment,there was an improvement in erectile *** expression of oxidative stress-related indicators was significantly increased and the NO/cGMP pathway was impaired in the DMED *** expression of proapoptotic factors was increased,while that of antiapoptotic factors was decreased in the DMED ***,the cell morphology in the cavernous tissue of the DMED group also changed ***,-OA treatment significantly reversed all these changes observed in the DMED *** conclusion,NO,-OA treatment partially improved erectile function in DMED rats through mechanisms that included inhibition of oxidative stress,activation of the NO/cGMP pathway,and a reduction in apoptosis.

关键词： apoptosis diabetic mellitus erectile dysfunction nitro-oleic acid NO/cGMP pathway oxidative stress

Passive particles driven by self-propelled particle:The wake effect

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Chinese Physics B 2024年第4期33卷 500-507页

作者：郑凯选汪静文王世锋聂德明 Institute of Fluid Mechanics China Jiliang UniversityHangzhou 310018China Department of Mechanical Engineering Hangzhou Vocational&Technical CollegeHangzhou 310018China

This work focuses on numerically studying hydrodynamic interaction between a passive particle and a self-propelled particle,termed a squirmer,by using a two-dimensional lattice Boltzmann method(LBM).It is found that the squirmer can capture a passive particle and propel it simultaneously,provided the passive particle is situated within the squirmer's *** research shows that the critical capture distance,which determines whether the particle is captured,primarily depends on the intensity of the squirmer's *** stronger dipolarity of squirmer results in an increased critical capture ***,the Reynolds number is found to have minimal influence on this ***,the passive particle,when driven by the squirmer's wake,contributes to a reduction in the squirmer's *** results in a mutual acceleration for both *** findings can provide valuable perspectives for formulating the principles of reducing the drag of micro-swimmers and help to achieve the goal of using micro-swimmers to transport goods without physical tethers.

关键词： lattice Boltzmann method(LBM) self-propelled particles particle-laden flow

Medical imaging for the diagnosis,recurrence and metastasis evaluation of clear cell sarcoma

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World Journal of Clinical Cases 2024年第29期12卷 6258-6261页

作者： wen-jing wang Xia wang Dong-Ming Hui Jun-Bang Feng Chuan-Ming Li Department of Medical Imaging Chongqing University Central HospitalChongqing 400014China Department of Radiology Chongqing Western HospitalChongqing 400050China

Clear cell sarcoma(CCS)of soft tissue is extremely rare,accounting for approximately 1%of all soft tissue *** is very difficult to diagnose CCS based on clinical *** resonance imaging(MRI)provides highresolution images of soft tissues and pathological features such as mucus,necrosis,bleeding,and fat through high and low signals on T1 weighted image(T1WI)and T2 weighted image(T2WI).On the other hand,the paramagnetism of melanin in CCS shortens the relaxation time of T1 and T2,and high signal intensity on T1WI and low signal intensity on T2WI can be *** is different from most other soft tissue *** present,the treatment method for CCS is surgical *** can effectively display the tumour edge,extent of surrounding oedema,and extent of fat involvement,which is highly important for guiding surgical resection and predicting postoperative *** an invasive sarcoma,CCS has a high risk of *** of the pathological condition of the resected tumour,MRI or computed tomography(CT)should be performed every 1-2 years to assess recurrence at the primary site and to screen for metastasis in the lungs,liver,and *** necessary,PET-CT can be performed to evaluate the overall condition of the patient.

关键词： Clear cell sarcoma Recurrence Metastasis Computed tomography Magnetic resonance imaging Editorial

Wedelolactone attenuates sepsis-associated acute liver injury by regulating the macrophage M1/M2 polarization balance through the PI3K/AKT/NF-κB signalling pathway

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Traditional Medicine Research 2024年第11期9卷 1-11页

作者： wang-Ting Li Jin-Yi Chen Shao-Jie Huang Dong-Mei Hu Xing-Ru Tao Fei Mu jing-Yi Zhao Chao Guo Jia-Lin Duan jing-wen wang Department of Pharmacology Shaanxi University of Chinese MedicineXianyang 712046China Department of Pharmacy Xijing HospitalFourth Military Medical UniversityXi’an 710032China Institute of Medical Research Northwestern Polytechnical UniversityXi’an 710032China.

Background:Liver injury caused by sepsis seriously impairs the normal physiology of the ***(WED)has an obvious anti-inflammatory effect against liver damage caused by various ***,further research is needed to determine if WED might mitigate acute liver damage linked to sepsis by influencing macrophage ***:We first assessed the effect of WED on lipopolysaccharides-triggered liver injury by biochemistry assay and tissue *** factors were assessed using the ELISA *** expression of Cluster of Differentiation 86(CD86)and Cluster of Differentiation 206(CD206)was measured by immunofluorescence *** protein levels of inducible nitric oxide sythase(iNOS),Arginase 1(Arg-1),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),PI3K phosphorylation(p-PI3K),AKT phosphorylation(p-AKT),inhibitor of kappa B kinase(IKK),inhibitor of kappa B(IκB),and nuclear factor kappa-B(NF-κB)p65 were quantified by western blot ***:WED decreased the level of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and malondialdehyde,and increased the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX).Moreover,WED exerted effective anti-inflammatory effects by decreasing the level of Tumor necrosis factor-α(TNF-α)and Interleukin 6(IL-6)and increasing the level of Interleukin 10(IL-10)in serum and *** not only decreased CD86 and iNOS expression but also increased CD206 and Arg-1 *** also downregulated the increased expression of PI3K,AKT,p-PI3K,p-AKT,IKK,and NF-κB p65 induced by lipopolysaccharides,while up-regulated the decreased expression of Iκ***,LY294002 with WED decreased the expression of protein PI3K,AKT,p-PI3K,p-AKT,IKK and NF-κB p65,and raised the expression of IκBα.Conclusion:Wedelolactone could attenuate sepsis-associated acute liver injury,and its mechanism may be associated with balancing pro-inflammatory and anti-inflamm

关键词： Wedelactone sepsis liver injury macrophage polarization PI3K/AKT/NF-κB

Gut microbiota in gastrointestinal diseases during pregnancy

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World Journal of Clinical Cases 2022年第10期10卷 2976-2989页

作者： Zhong-Zhen Liu jing-Hua Sun wen-jing wang BGI-Shenzhen Shenzhen 518083Guangdong ProvinceChina College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Gut microbiota(GM)is a micro-ecosystem composed of all microorganisms in the human *** interaction between GM and the host plays an important role in maintaining normal physiological functions in the *** of the GM may cause various *** has been demonstrated to be associated with human health and disease,and changes during individual development and *** is a complicated physiological ***,the immune system,metabolism,and GM undergo drastic changes during *** diseases during pregnancy,such as hepatitis,intrahepatic cholestasis of pregnancy,and pre-eclampsia,can affect both maternal and fetal *** dysregulation of GM during pregnancy may lead to a variety of diseases,including gastrointestinal ***,we review recent research articles on GM in pregnancy-related gastrointestinal diseases,discuss the interaction of the GM with the host under normal physiological conditions,gastrointestinal diseases,and pregnancy-specific *** more attention is paid to reproductive health,the pathogenic mechanism of GM in gastrointestinal diseases during pregnancy will be further studied to provide a theoretical basis for the use of probiotics to treat these diseases.

关键词： Gut microbiota Microbiome Pregnancy Gastrointestinal diseases Hormones Immunity Metabolites

The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2022年第3期17卷 594-600页

作者： jing-Jun Zhao Zheng-Hui wang Ying-Jie Zhang wen-jing wang Ai-Fang Cheng Pei-jing Rong Chun-Lei Shan Center of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina Center of Rehabilitation Medicine the First Affiliated Hospital of Xinxiang Medical UniversityXinxiangHenan ProvinceChina Shanghai Research Institute of Acupuncture and Meridian ShanghaiChina Institute of Acupuncture and Moxibustion Chinese Academy of Chinese Medical SciencesBeijingChina

Previous studies have shown that vagus nerve stimulation can improve patients' locomotor *** stimulation of the auricular vagus nerve,which is the only superficial branch of the vagus nerve,may have similar effects to vagus nerve ***,the precise mechanisms remain poorly *** this study,rat models of cerebral ischemia/reperfusion injury were established by modified Longa ***-four hours later,7-day auricular vagus nerve stimulation was *** results showed that auricular vagus nerve stimulation promoted the secretion of acetylcholine,inhibited the secretion of interleukin-1β,interleukin-6,and tumor necrosis factor-α,and reduced connexin 43 phosphorylation in the ischemic penumbra and motor cortex,promoting locomotor function recovery in rats with cerebral ischemia/reperfusion *** findings suggested that auricular vagus nerve stimulation promotes the recovery of locomotor function in rats with cerebral ischemia/reperfusion injury by altering the secretion of acetylcholine and inflammatory factors and the phosphorylation of connexin *** study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine on November 8,2019(approval ***191108014).

关键词： auricular vagus nerve stimulation connexin 43 gap junction inflammatory factor ischemia/reperfusion injury neurological function neuroprotection stroke

Highly efficient generation of blastocyst-like structures from spliceosomes-repressed mouse totipotent blastomere-like cells

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Science China(Life Sciences) 2023年第3期66卷 423-435页

作者： Pengfei Zhang Xuzhao Zhai Boyan Huang Shu Sun wen jing wang Man Zhang GMU-GIBH Joint School of Life Sciences The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and DiseasesThe Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou LaboratoryGuangzhou Medical UniversityGuangzhou 510005China Center for Cell Lineage and Atlas(CCLA) Bioland LaboratoryGuangzhou Regenerative Medicine and Health Guangdong LaboratoryGuangzhou 510005China

Mammalian embryogenesis begins with a totipotent ***-like structures can be captured by aggregated cells with extended pluripotent properties in a three-dimensional(3D)culture ***,the efficiency of generating blastoids is low,and it remains unclear whether other reported totipotent-like stem cells retain a similar *** this study,we demonstrated that spliceosomal repression-induced totipotent blastomere-like cells(TBLCs)form blastocyst-like structures within around 80%of all *** addition,we generated blastoids initiating from a single ***-blastoids express specific markers of constituent cell lineages of a blastocyst and resemble blastocyst in cell-lineage ***,singlecell RNA sequencing revealed that TBLC-blastoids share a similar transcriptional profile to natural embryos,albeit composed of fewer primitive endoderm-like ***,TBLC-blastoids can develop beyond the implantation stage in vitro and induce decidualization in *** summary,our findings provided an alternative cell type to efficiently generate blastoids for the study of early mouse embryogenesis.

关键词： blastoids TBLCs early embryogenesis totipotent

Nucleic acid drug vectors for diagnosis and treatment of brain diseases

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Signal Transduction and Targeted Therapy 2023年第2期8卷 604-656页

作者： Zhi-Guo Lu Jie Shen Jun Yang jing-wen wang Rui-Chen Zhao Tian-Lu Zhang jing Guo Xin Zhang State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing100190P.R.China University of Chinese Academy of Sciences Beijing100049P.R.China

Nucleic acid drugs have the advantages of rich target selection,simple in design,good and enduring *** have been demonstrated to have irreplaceable superiority in brain disease treatment,while vectors are a decisive factor in therapeutic *** physiological barriers,such as degradation and clearance in circulation,blood-brain barrier,cellular uptake,endosome/lysosome barriers,release,obstruct the delivery of nucleic acid drugs to the brain by the *** acid drugs against a single target are inefficient in treating brain diseases of complex *** between individual patients lead to severe uncertainties in brain disease treatment with nucleic acid *** this Review,we briefly summarize the classification of nucleic acid ***,we discuss physiological barriers during drug delivery and universal coping strategies and introduce the application methods of these universal strategies to nucleic acid drug ***,we explore nucleic acid drug-based multidrug regimens for the combination treatment of brain diseases and the construction of the corresponding *** the following,we address the feasibility of patient stratification and personalized therapy through diagnostic information from medical imaging and the manner of introducing contrast agents into ***,we take a perspective on the future feasibility and remaining challenges of vector-based integrated diagnosis and gene therapy for brain diseases.

关键词： treatment diagnosis replace