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The Polycomb Complex PRC1:Composition and Function in Plants

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Journal of Genetics and Genomics 2013年第5期40卷 231-238页

作者： Anne Molitor wen-hui shen Institut de Biologie Moléculaire des Plantes du CNRS Université de Strasbourg12 rue du Général Zimmer67084 StrasbourgFrance Institut J.-P.Bourgin UMR1318 INRA-AgroParisTechINRAroute de Saint Cyr78026 VersaillesFrance

Polycomb group（PcG） proteins are crucial epigenetic regulators conferring transcriptional memory to cell *** assemble into multi-protein complexes,e.g.,Polycomb Repressive Complex 1 and 2（PRC1,PRC2）,which are thought to act in a sequential manner to stably maintain gene ***2 induces histone H3 lysine 27（H3K27） trimethylation（H3K27me3）,which is subsequently read by PRCl that further catalyzes H2A monoubiquitination（H2Aub1）,creating a transcriptional silent chromatin ***2 components are conserved in plants and have been extensively characterized in *** contrast,PRCl composition and function are more diverged between animals and *** more recently,PRC1 existence in plants has been *** we review the aspects of plant specific and conserved PRC1 and highlight critical roles of PRC1 components in seed embryonic trait determinacy,shoot stem cell fate determinacy,and flower development in Arabidopsis.

关键词： Epigenetics Chromatin Histone modifications Polycomb PRC1 Arabidopsis development

A Truncated Arabidopsis NUCLEOSOME ASSEMBLY PROTEIN 1, AtNAP1;3T, Alters Plant Growth Responses to Abscisic Acid and Salt in the Atnap 1;3-2 Mutant

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Molecular Plant 2009年第4期2卷 688-699页

作者： Zi-Qiang Liu Juan Gao Ai-Wu Dong wen-hui shen Institut de Biologie Moleculaire des Plantes (IBMP) du CNRS Universite de Strasbourg (UdS) 12 rue du General Zimmer 67084 Strasbourg Cedex France State Key Laboratory of Genetic Engineering Institute of Plant Biology Department of Biochemistry School of Life Sciences Fudan University Shanghai 200433 People's Republic of China

Chromatin remodeling is thought to have crucial roles in plant adaptive response to environmental stimulus. Here, we report that, in Arabidopsis, the evolutionarily conserved histone chaperone, NUCLEOSOME ASSEMBLY PROTEIN 1 （NAP1）, is involved in plant response to abscisic acid （ABA）, a phytohormone important in stress adaptation. We show that simultaneous loss-of-function of AtNAP1;1, AtNAP1;20 and AtNAP1;3 （the triple mutant m123-1） caused a slight hypersensitive response to ABA in seedling growth. Strikingly, the other triple mutant m123-2 containing a different mutant allele of AtNAP1;3, the Atnap 1;3-2 allele, showed a hyposensitive response to ABA and a decreased tolerance to salt stress. This ABA- hyposensitive and salt response phenotype specifically associated with the Atnapl;3-2 mutant allele. We show that this mutant allele produced a truncated protein, AtNAP1;3T, which lacks 34 amino acids at the C-terminus compared to the wild-type protein AtNAP1;3. We further show that the heterozygous plants containing the Atnapl;3-2 mutant allele as well as transgenic plants overexpressing AtNAP1;3Texhibit ABA-hyposensitive phenotype. It thus indicates that AtNAP1;3T functions as a dominant negative factor in ABA response. The expression of some ABA-responsive genes, including genes encoding protein kinases and transcription regulators, was found perturbed in the mutant and in theAtNAP1;3Ttransgenic plants. Taken together, our study uncovered AtNAP1 proteins as positive regulators and AtNAP1;3Tas a negative regulator in ABA signaling pathways, providing a novel link of chromatin remodeling to hormonal and stress responses.

关键词： Histone chaperone chromatin epigenetics abiotic stress Arabidopsis.

Chromatin-remodeling factor OsINO80 is involved in regulation of gibberellin biosynthesis and is crucial for rice plant growth and development

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Journal of Integrative Plant Biology 2018年第2期60卷 144-159页

作者： Chao Li Yuhao Liu wen-hui shen Yu Yu Aiwu Dong State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and Development Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China Institut de Biologie Moléculaire des Plantes UPR2357 CNRSUniversité de Strasbourg 12 rue du General Zimmer 67084 Strasbourg CedexFrance

The phytohormone gibberellin（GA） plays essential roles in plant growth and development. Here,we report that OsINO80, a conserved ATP-dependent chromatin-remodeling factor in rice（Oryza sativa）, functions in both GA biosynthesis and diverse biological processes. OsINO80-knockdown mutants, derived from either T-DNA insertion or RNA interference, display typical GA-deficient phenotypes, including dwarfism, reduced cell length, late flowering, retarded seed germination and impaired reproductive development. Consistently, transcriptome analyses reveal that OsINO80 knockdown results in downregulation by more than two-fold of over 1,000 genes, including the GA biosynthesis genes CPS_1 and GA_3ox_2, and the dwarf phenotype of OsINO80-knockdown mutants can be rescued by the application of exogenous GA3. Chromatin immunoprecipitation（Ch IP） experiments show that OsINO80 directly binds to the chromatin of CPS1 and GA_3ox_2 loci. Biochemical assays establish that OsINO80 specially interacts with histone variant H_2A.Z and the H_2A.Z enrichments at CPS_1 and GA_3ox_2 are decreased in OsINO80-knockdown mutants. Thus, our study identified a rice chromatin-remodeling factor,OsINO80, and demonstrated that OsINO80 is involved in regulation of the GA biosynthesis pathway and plays critical functions for many aspects of rice plant growth and development.

关键词： GA Chromatin-remodeling factor OsINO80 is involved in regulation of gibberellin biosynthesis and is crucial for rice plant growth and development Figure

A Non-canonical Transferred DNA Insertion at the BRI1 Locus in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2009年第4期51卷 367-373页

作者： Zhong Zhao Yan Zhu Mathieu Erhardt Ying Ruan wen-hui shen Pre-National Laboratory of Crop Germplasm Innovation and Utilization Hunan Agricultural University Changsha 410128 China Institut de Biologie Moléculaire des Plantes du CNRS (UPR 2357) Université de Strasbourg (LIDS) 12 rue du Général Zimmer 67084 Strasbourg cedex France

Agrobacterium-mediated transformation is widely used in transgenic plant engineering and has been proven to be a powerful tool for insertional mutagenesis of the plant gsnome. The transferred DNA （T-DNA） from Agrobacterium is integrated into the plant genome through illegitimate recombination between the T-DNA and the plant DNA. Contrasting to the canonical insertion, here we report on a locus showing a complex mutation associated with T-DNA insertion at the BRI1 gene in Arabidopsis thaliana. We obtained a mutant line, named salads for its phenotype of dwarf stature and proliferating rosette. Molecular characterization of this mutant revealed that in addition to T-DNA a non-T-DNA-Iocalized transposon from bacteria was inserted in the Arabidopsis genome and that a region of more than 11.5 kb of the Arabidopsis genome was deleted at the insertion site. The deleted region contains the braesinosteroid receptor gene BRI1 and the transcription factor gene WRKY13. Our finding reveals non-canonical T-DNA insertion, implicating horizontal gene transfer and cautioning the use of T-DNA as mutagen in transgenic research.

关键词： Agrobacterium Arabidopsis BRI1 gene flux transposon Tn5393 T-DNA transformation.

H3K36 Methylation Is Involved in Promoting Rice Flowering

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Molecular Plant 2013年第3期6卷 975-977页

作者： Pengfei Sui Jinlei Shi xueying Gao wen-hui shen Aiwu -Dong State Key Laboratory of Genetic Engineering Institute of Plant Biology Department of Biochemistry School of Life Sciences Fudan University Shanghai 200433 RR. China Institut de Biologie Moleculaire des Plantes du CNRS Universite de Strasbourg 12 rue du General Zimmer 67084 Strasbourg Cedex France

Dear Editor, Histone H3 lysine 36 （H3K36） methylation is a conserved epigenetic mark in all eukaryotes （Berr et al., 2011; Wagner and Carpenter, 2012）. Reverse genetic analysis in Arabidopsis had uncovered a crucial role of H3K36 di- and tri-methylation （H3K36me2 and H3K36me3） in flowering-time regulation （reviewed in Berr et al., 2011）.

关键词： H3K36 Methylation Involved

SDG714 Regulates Specific Gene Expression and Consequently Affects Plant Growth via H3K9 Dimethylation

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Journal of Integrative Plant Biology 2010年第4期52卷 420-430页

作者： Bo Ding Yan Zhu Zhong-Yuan Bu wen-hui shen Yu Yu Ai-Wu Dong State KeyLaboratory of Genetic Engineering Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China Institut de Biologie Moleculaire des Plantes du CNRS Universite Louis Pasteur de Strasbourg 67084 Strasbourg Cedex France B. Ding and Y. Zhu contributed equally to this paper

Histone lysine methylation is known to be involved in the epigenetic regulation of gene expression in all eukaryotes including plants. Here we show that the rice SDG714 is primarily responsible for dimethylation but not trimethylaUon on histone H3K9 in vivo. Overexpression of YFP-SDG714 in Arabidopsis significantly inhibits plant growth and this inhibition is associated with an enhanced level of H3K9 dimethylation. Our microarray results show that many genes essential for the plant growth and development were downregulated in transgenic Arabidopsis plants overexpressing YFP-SDG714. By chromatin immunoprecipitation analysis, we show that YFP-SDG714 is targeted to specific chromatin regions and dimethylate the H3Kg, which is linked with heterochromatinization and the downregulation of genes. Most interestingly, when YFP-SDG714 production is stopped, the inhibited plants can partially restore their growth, suggesting that the perturbation of gene expression caused by YFP-SDG714 is revertible. Taken together, our results point to an important role of SDG714 in H3K9 dimethylation, suppression of gene expression and plant growth, and provide a potential method to regulate gene expression and plant development by an on-off switch of SDG714 expression.

关键词： Specific Gene Expression Consequently Affects

Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis

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Journal of Integrative Plant Biology 2016年第12期58卷 959-970页

作者： Zhongfei Li Bin Li Jian Liu Zhihao Guo Yuhao Liu Yan Li wen-hui shen Ying Huang Hai Huang Yijing Zhang Aiwu Dong State Key Laboratory of Genetic Engineering International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research Collaborative Innovation Center for Genetics and Development Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China National Center for Protein Science Shanghai State Key Laboratory of Molecular Biology Shanghai State Key Laboratory of Molecular Andrology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China Institut de Biologie Moléculaire des Plantes du CNRS Université de Strasbourg 12 rue du General Zimmer 67084 Strasbourg Cedex France

Polycomb group proteins are important repressors of numerous genes in higher eukaryotes. However, the mechanism by which Polycomb group proteins are recruited to specific genes is poorly understood. In Arabidopsis, LIKE HETEROCHROMATIN PROTEIN 1 （LHP1）, also known as TERMINAL FLOWER 2, was originally proposed as a subunit of polycomb repressive complex 1 （PRC1） that could bind the tri-methylated lysine 27 of histone H3 （H3K27me3） established by the PRC2. In this work, we show that LHP1 mainly functions with PRC2 to establish H3K27me3, but not with PRC1 to catalyze monoubiquitination at lysine 119 of histone H2A. Our results show that complexes of the transcription factors ASYMMETRIC LEAVES 1 （AS1） and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED＇t-like homeobox （KNOX） genes BREVIPEDICELLU5 and KNAT2 via direct interactions with LHP1. Additionally, our transcriptome analysis indicated that there are probably more common target genes of AS1 and LHP1 besides Class-I KNOX genes during leaf development in Arabidopsis.

关键词： Arabidopsis thaliana ASYMMETRIC LEAVES 1

Determination of Blood Indices of Guide Black-Fur Sheep in Qinghai Province of China

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Animal Husbandry and Feed Science 2009年第11期1卷 13-15页

作者： ZOU wen-hui shen Lei School of Life Science and Engineering Northwest University for Nationalities Lanzhou 730030 China

[ Objective] The research was aimed to provide the basis for the development and utilization of Guide black-fur sheep and its disease di- agnosis and prevention. [ Methed] Blood samples were collected from 15 healthy adult Guide black-fur sheep in Black-Fur Sheep Farm o＇f Guinan County in Qinghai Province. Eighteen blood indices belonging to three classes, erythrocyte, leukocyte and platelet, were determined. E Resultl The blood indices of the Guide black-fur sheep were as follows： the white blood cell count, （7.52 ±2.72） × 109/L; the red blood cell count, （13.04 ± 2.81 ） × 1012/L; the mean corpuscular hemoglobin, （85.36 ＋27.05） g/L; the mean corpuscular volume, （37.22 ± 1.93） fL; the mean corpuscular hemoglobin concentration, （13.19 ±0.96） pg; the platelet count, （370.67 ± 139.60） × 109/L; the lymphocyte absolute count, （14.11 ＋9.36） x 109/L; the absolute count of intermediate cell, （0.79 ±0.46）× 109/L; and the absolute neutrophil count, （0.81 ＋0.71 ） ×109/L.- Conclusion] The reseamh lays the foundation for further Drotectina the aenetic resources of the Guide black-fur sheep and imDrovinQ their production performance.

关键词： Guide black-fur sheep Blood indices Determination

Multifunctional carbon materials from rugose rose for energy storage and water purification

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Frontiers of Chemical Science and Engineering 2024年第9期18卷 1-13页

作者： Peng-hui Li hui Zhou wen-Juan Wu Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources Nanjing Forestry UniversityNanjing 210037China College of Light Industry and Food Engineering Nanjing Forestry UniversityNanjing 210037China College of Chemical Engineering East China University of Science and TechnologyShanghai 200237China State Key Laboratory of Chemical Engineering Shanghai 200237China

With the advancement of social process,the resource problem is becoming more prominent,biomass materials come into being,and it is becoming more and more important to explore and prepare efficient and multifunctional biomass materials to alleviate the problems of energy storage and water *** this paper,nitrogen-doped hierarchical porous carbon materials(NRRC)were produced by one-step carbonization of withered rose as raw material and melamine as nitrogen source with KOH-activated *** resulting nitrogen-doped porous carbon material had the most abundant pores and the best microspherical graded pore structure,with a specific surface area of up to 1393 m2·g^(-1),a pore volume of 0.68 cm3·g^(-1),and a nitrogen-doped content of 5.52%.Electrochemical tests showed that the maximum specific capacitance of NRRC in the three-electrode system was 346.4 F·g^(-1)(0.5 A·g^(-1)),which was combined with favorable capacitance retention performance and cycling *** NRRC//NRRC symmetric supercapacitors were further assembled,and the maximum energy density of a single device was 23.88 Wh·kg^(-1),which still maintains excellent capacitance retention and cyclic charging/discharging *** example,the capacitance retention rate was always close to 96.27%with almost negligible capacitance loss after 10000 consecutive charge/discharge cycles(current density:10 A·g^(-1)).Regardless of the three-electrode or two-electrode system,the super capacitive performance of NRRC porous carbon materials was comparable to the electrochemical performance of many reported biomass porous carbon materials,which showed better energy storage advantages and practical application *** addition,NRRC porous carbon materials had excellent water purification *** dye adsorption test confirmed that NRRC had a high adsorption capacity(491.47 mg·g^(-1))for methylene *** undoubtedly also showed a potential and promising avenue for high value-added utilization

关键词： rose carbon adsorption electrode material electrochemical performance