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Dextran sulfate sodium-induced colitis-associated neoplasia:a promising model for the development of chemopreventive interventions

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Acta Pharmacologica Sinica 2007年第9期28卷 1450-1459页

作者： Margie Lee CLAPPER Harry Stanley COOPER wen-chi Lee chang Divisions of Population Science Medical Science Fox Chase Cancer CenterPhiladelphiaPA 19111USA

Individuals diagnosed with ulcerative colitis face a significantly increased risk ofdeveloping colorectal dysplasia and cancer during their *** date,littleattention has been given to the development of a chemopreventive interventionfor this high-risk *** mouse model of dextran sulfate sodium (DSS)-induced colitis represents an excellent preclinical system in which to both charac-terize the molecular events required for tumor formation in the presence of inflam-mation and assess the ability of select agents to inhibit this *** admin-istration of DSS in drinking water results in the establishment of chronic colitisand the development of colorectal dysplasias and cancers with pathological fea-tures that resemble those of human colitis-associated *** incidenceand multiplicity of lesions observed varies depending on the mouse strain used(ie,Swiss Webster,C57BL/6J,CBA,ICR) and the dose (0.7%-5.0%) and schedule(1-15 cycles with or without a subsequent recovery period) of *** incidenceof neoplasia can be increased and its progression to invasive cancer acceleratedsignificantly by administering DSS in combination with a known colon carcinogen(azoxymethane (AOM),2-amino-3-methylimidazo[4,5-f]quinoline (IQ),2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)) or *** recent induction ofcolitis-associated neoplasia in genetically defined mouse strains has providednew insight into the role of specific genes (ie,adenomatous polyposis coli (Apc),p53,inducible nitric oxide synthase (iNOS),Msh2) in the development of colitis-associated *** data from chemopreventive intervention studiesdocument the efficacy of several agents in inhibiting DSS-induced neoplasiaand provide great promise that colitis-associated colorectal neoplasia is a pre-ventable disease.

关键词： colorectal colitis chemoprevention dextran sulfate sodium mouse models

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SuccSite:Incorporating Amino Acid Composition and Informative k-spaced Amino Acid Pairs to Identify Protein Succinylation Sites

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Genomics, Proteomics & Bioinformatics 2020年第2期18卷 208-219页

作者： Hui-Ju Kao Van-Nui Nguyen Kai-Yao Huang wen-chi chang Tzong-Yi Lee Department of Computer Science and Engineering Yuan Ze UniversityTaoyuan 32003TaiwanChina Department of Information Technology University of Information and Communication TechnologyThai Nguyen 1000Vietnam School of Science and Engineering The Chinese University of Hong KongShenzhen 518172China Warshel Institute for Computational Biology The Chinese University of Hong KongShenzhen 518172China Institute of Tropical Plant Sciences Cheng Kung UniversityTainan 701TaiwanChina

Protein succinylation is a biochemical reaction in which a succinyl group(-CO-CH2-CH2-CO-)is attached to the lysine residue of a protein *** succinylation plays important regulatory roles in living ***,studies in this field are limited by the difficulty in experimentally identifying the substrate site specificity of lysine *** facilitate this process,several tools have been proposed for the computational identification of succinylated lysine *** this study,we developed an approach to investigate the substrate specificity of lysine succinylated sites based on amino acid *** experimentally verified lysine succinylated sites collected from public resources,the significant differences in position-specific amino acid composition between succinylated and non-succinylated sites were represented using the Two Sample Logo *** findings enabled the adoption of an effective machine learning method,support vector machine,to train a predictive model with not only the amino acid composition,but also the composition of k-spaced amino acid *** the selection of the best model using a ten-fold crossvalidation approach,the selected model significantly outperformed existing tools based on an independent dataset manually extracted from published research ***,the selected model was used to develop a web-based tool,SuccSite,to aid the study of protein *** proteins were used as case studies on the website to demonstrate the effective prediction of succinylation *** will regularly update SuccSite by integrating more experimental *** is freely accessible at http://***/SuccSite/.

关键词： Protein succinylation Succinyl group Substrate specificity Amino acid composition k-spaced amino acid pair composition

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Deciphering mycorrhizal fungi in cultivated Phalaenopsis microbiome with next-generation sequencing of multiple barcodes

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Fungal Diversity 2014年第3期66卷 77-88页

作者： Chao-Li Huang Feng-Yin Jian Hao-Jen Huang wen-chi chang wen-Luan Wu chi-Chuan Hwang Ruey-Hua Lee Tzen-Yuh chiang Department of Life Sciences National Cheng Kung UniversityTainanTaiwan 701 Institute of Tropical Plant Sciences National Cheng Kung UniversityTainanTaiwan 701 Department of Engineering Science and Supercomputing Research Center National Cheng Kung UniversityTainanTaiwan 701

Identifying the species composition of a microbial ecosystem is often hampered by difficulties in culturing the organisms and in the low sequencing depth of traditional DNA *** analysis,a huge-scale nucleotide-sequence-based tool,can overcome such *** this study,Sanger sequencing of 500 nrITS clones uncovered 29 taxa of 19 fungal genera,whereas metagenomics with next-generation sequencing identified 512 operational taxonomic units(OTUs)for ITS1/2 and 364 for ITS3/***,high throughput sequencing of PCR amplicons of ITS1/2,ITS3/4,nrLSU-LR,nrLSU-U,mtLSU,and mtATP6,all with at least 1,300×coverage and about 21 million reads in total,yielded a very diverse fungal *** fact that 74%of the OTUs were exclusively uncovered with single barcodes indicated that each marker provided its own insights into the fungal *** deal with the high heterogeneity in the data and to integrate the information on species composition across barcodes,a rank-scoring strategy was ***,205 genera among 64 orders of fungi were identified in healthy Phalaenopsis *** the barcodes utilized,ITS1/2,ITS3/4,and nrLSU-U were the most competent in uncovering the fungal *** barcodes,though detecting different compositions likely due to primer preference,provided complementary and comprehensive power in deciphering the microbial diversity,especially in revealing rare species.

关键词： DNA barcode Metagenomics Mycorrhizal fungi Next-generation sequencing Phalaenopsis orchid Rank scoring

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Verification of gene expression profiles for colorectal cancer using 12 internet public microarray datasets

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World Journal of Gastroenterology 2014年第46期20卷 17476-17482页

作者： Yu-Tien chang Chung-Tay Yao Sui-Lung Su Yu-ching Chou chi-Ming Chu chi-Shuan Huang Harn-Jing Terng Hsiu-Ling Chou Thomas Wetter Kang-Hua Chen chi-wen chang Yun-wen Shih ching-Huang Lai Division of Biomedical Statistics and Informatics School of Public HealthNational Defense Medical Center Department of Emergency Cathay General Hospital Department of Epidemiology School of Public HealthNational Defense Medical Center Division of Colorectal Surgery Cheng Hsin Rehabilitation Medical Center Advpharma Inc. Department of Nursing Far Eastern Memorial Hospital Oriental Institute of Technology Department of Medical Informatics Faculty of MedicineUniversity of Heidelberg69120 HeidelbergGermany School of Nursing College of MedicineChang Gung University

AIM: To verify gene expression profiles for colorectal cancer using 12 internet public microarray datasets.

关键词： Gene expression profiles Colorectal cancer Microarray Gene Expression Omnibus Gene Expression Omnibus Gene Expression Omnibus series

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Gene expression profile of peripheral blood in colorectal cancer

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World Journal of Gastroenterology 2014年第39期20卷 14463-14471页

作者： Yu-Tien chang chi-Shuan Huang Chung-Tay Yao Sui-Lung Su Harn-Jing Terng Hsiu-Ling Chou Yu-ching Chou Kang-Hua Chen Yun-wen Shih chian-Yu Lu ching-Huang Lai Chen-En Jian chiao-Huang Lin chien-Ting Chen Yi-Syuan Wu Ke-Shin Lin Thomas Wetter chi-wen chang chi-Ming Chu Division of Biomedical Statistics and Informatics School of Public Health National Defense Medical Center Division of Colorectal Surgery Cheng Hsin Rehabilitation Medical Center Department of Surgery Cathay General Hospital Department of Epidemiology School of Public Health National Defense Medical Center Advpharma Inc. Department of Nursing Far Eastern Memorial Hospital and Oriental Institute of Technology Department of Nursing School of Medicine Chang Gung University Air Force Combatant Command National Defense Ministry Institute of Medical Biometry and Informatics Heidelberg University Germany Department of Biomedical Informatics and Medical Education University of Washington Seattle United States

AIM: Optimal molecular markers for detecting colorectal cancer (CRC) in a blood-based assay were evaluated.

关键词： Colorectal cancer Gene expression Microarray Internet

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The Study of the Management Competence Gap Analysis： Top and Middle Managers of Taipei 101

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Journal of Modern Accounting and Auditing 2011年第10期7卷 1122-1127页

作者： Yu chin-Cheh Liao Pei-wen Chao chia-chi chang chin-Shin National Taiwan Normal University Taiwan China

Business has managerial talent showing great foresight. High quality human resources management is the way to attain high performance and a competitive advantage. This study uses documents, interviews, expert opinions and questionnaires to discuss management competence of the Taipei 101. Finally, we propose ways to supply Taipei 101 T＆D, and improve managers＇ competence.

关键词： Taipei 101 management competence

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Interconnections between diabetic corneal neuropathy and diabetic retinopathy:diagnostic and therapeutic implications

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Neural Regeneration Research 2025年第8期20卷 2169-2180页

作者： Mingyi Yu Faith Teo En Ning chang Liu Yu-chi Liu Tissue Engineering and Stem Cell Group Singapore Eye Research InstituteSingapore Yong Loo Lin School of Medicine National University of SingaporeSingapore Department of Cornea and External Eye Disease Singapore National Eye CenterSingapore Ophthalmology and Visual Sciences Academic Clinical Program Duke-NUS Medical SchoolSingapore

Diabetic corneal neuropathy and diabetic retinopathy are ocular complications occurring in the context of diabetes *** corneal neuropathy refers to the progressive damage of corneal *** retinopathy has traditionally been considered as damage to the retinal ***,growing evidence suggests that diabetic retinopathy is a complex neurovascular disorder resulting from dysfunction of the neurovascular unit,which includes both the retinal vascular structures and neural *** retinopathy is one of the leading causes of blindness and is frequently screened for as part of diabetic ocular ***,diabetic corneal neuropathy is commonly overlooked and underdiagnosed,leading to severe ocular surface *** studies have found that these two conditions tend to occur together,and they share similarities in their pathogenesis pathways,being triggered by a status of chronic *** review aims to discuss the interconnection between diabetic corneal neuropathy and diabetic retinopathy,whether diabetic corneal neuropathy precedes diabetic retinopathy,as well as the relation between the stage of diabetic retinopathy and the severity of corneal *** also endeavor to explore the relevance of a corneal screening in diabetic eyes and the possibility of using corneal nerve measurements to monitor the progression of diabetic retinopathy.

关键词： cornea corneal nerves diabetes diabetic corneal neuropathy diabetic retinopathy retina

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Metal-organic frameworks with dinuclear metal centers for synergistically boosting CO2 photoreduction

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Science china Chemistry 2025年第1期68卷 201-208页

作者： Hui-Feng Wang wen-Jie Shi Yu-Xin Yang Bao-Xia Dong Tong-Bu Lu Di-chang Zhong School of Chemistry and Chemical Engineering Yangzhou University Institute for New Energy Materials and Low Carbon Technologies School of Materials Science and Engineering Tianjin University of Technology

Dinuclear metal synergistic catalysis （DMSC） has been proved powerful in boosting CO2reduction,while this has not been achieved in heterogeneous catalysts with long-range order *** this study,we,by introduction of metal catalytic centers into an indium-based metal-organic framework （MOF）,obtained a series of isostructural In-MOFs,including three MOFs with mononuclear metal catalytic center （M-In）,three MOFs with dinuclear homometallic catalytic centers （MM-In）,and three MOFs with dinuclear heterometallic catalytic centers (MM’-In,M/M’=Co（II）,Ni（II）,Zn（II）).It was found that all these MOFs can serve as catalysts for photochemical CO2reduction to *** with M-In,the catalytic activities of MM-In were obviously enhanced,and that of MM’-In was further significantly *** example,the CO yield of Co Zn-In reaches a record value of 12,700μmol g-1h-1,which is 4.0,8.1,9.4,and 18.6-fold higher than that of Co Co-In,Zn Zn-In,Co-In,and Zn-In,***,the catalytic activities of Co Ni-In and Ni Zn-In also show different fold increase than those of corresponding dinuclear homometallic and mononuclear metallic *** results of control experiments and theoretical calculation reveal that the higher catalytic activities of MM-In over M-In can be ascribed to the DMSC effect,and the higher catalytic activity of MM’-In over MM-In can be ascribed to the strengthened DMSC *** work reveals the relationship between DMSC effect and bimetal centers in CO2reduction,which provides new insights into the DMSC and paves a new way to design highly active catalysts for photochemical CO2reduction.

关键词： metal-organic frameworks dinuclear metal centers synergistic catalysis CO2 reduction photocatalysis

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Plecanatide-mediated activation of guanylate cyclase-C suppresses inflammation-induced colorectal carcinogenesis in Apc+/Min-FCCC mice

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2017年第1期8卷 47-59页

作者： wen-chi L chang Shet Masih Anusha Thadi Viren Patwa Apoorva Joshi Harry S Cooper Vaseem A Palejwala Margie L Clapper Kunwar Shailubhai Cancer Prevention and Control Program Fox Chase Cancer Center Baruch S.Blumberg Institute Department of Pathology Fox Chase Cancer Center R and D Center Synergy Pharmaceuticals Inc.

AIM To evaluate the effect of orally administered plecanatide on colorectal dysplasia in Apc^(+/Min-FCCC) mice with dextran sodium sulfate(DSS)-induced inflammation. METHODS Inflammation driven colorectal carcinogenesis was induced in Apc^(+/Min-FCCC) mice by administering DSS in their drinking water. Mice were fed a diet supplemented with plecanatide(0-20 ppm) and its effect on the multiplicity of histopathologically confirmed polypoid,flat and indeterminate dysplasia was evaluated. Plecanatide-mediated activation of guanylate cyclase-C(GC-C) signaling was assessed in colon tissues by measuring cyclic guanosine monophosphate(cG MP) by ELISA, protein kinase G-II and vasodilator stimulated phosphoprotein by immunoblotting. Ki-67, c-myc and cyclin D1 were used as markers of proliferation. Cellular levels and localization of b-catenin in colon tissues were assessed by immunoblotting and immunohistochemistry, respectively. Uroguanylin(UG) and GC-C transcript levels were measured by quantitative reverse transcription polymerase chain reaction(RT-PCR). A mouse cytokine array panel was used to detect cytokines in the supernatant of colon explant cultures. RESULTS Oral treatment of Apc^(+/Min-FCCC) mice with plecanatide produced a statistically significant reduction in the formation of inflammation-driven polypoid, flat and indeterminate dysplasias. This anti-carcinogenic activity of plecanatide was accompanied by activation of cG MP/GC-C signaling mediated inhibition of Wnt/b-catenin signaling and reduced proliferation. Plecanatide also decreased secretion of pro-inflammatory cytokines(IL-6, IL-1 TNF), chemokines(MIP-1, IP-10) and growth factors(GCSF and GMCSF) from colon explants derived from mice with acute DSS-induced inflammation. The effect of plecanatidemediated inhibition of inflammation/dysplasia on endogenous expression of UG and GC-C transcripts was measured in intestinal tissues. Although GC-C expression was not altered appreciably, a statistically significant i

关键词： Guanylate cyclase-C Uroguanylin Plecanatide Inflammation Colorectal cancer

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曼氏无针乌贼(Sepiella japonica)精子表面蛋白17(Sp17)基因克隆以及组织表达特异性分析

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海洋与湖沼 2017年第5期48卷 1044-1051页

作者：周林李颖吕振明史会来吴常文迟长凤浙江海洋大学海洋科学与技术学院国家海洋设施养殖工程技术研究中心海洋生物种质发掘与利用国家地方联合实验室舟山316022 浙江省海洋水产研究所舟山316021

精子表面蛋白17(Sp17)是参与受精过程的关键分子。本研究采用RT-PCR以及RACE技术克隆得到了曼氏无针乌贼(Sepiella japonica)Sp17简称Sj-Sp17 c DNA的全长序列,序列全长1463bp,5′和3′非编码区分别为92bp和222bp,预测的开放阅读框(ORF... 详细信息

精子表面蛋白17(Sp17)是参与受精过程的关键分子。本研究采用RT-PCR以及RACE技术克隆得到了曼氏无针乌贼(Sepiella japonica)Sp17简称Sj-Sp17 c DNA的全长序列,序列全长1463bp,5′和3′非编码区分别为92bp和222bp,预测的开放阅读框(ORF)全长1149bp。编码的蛋白理论分子量为42.0548k Da,等电点4.66,是一种亲水性蛋白,不存在跨膜区以及信号肽序列,含有丰富的螺旋结构(54%)。同源氨基酸序列比对发现,与其他软体动物的相似性最高仅为44%,表明Sp17在进化中并不保守。基于Sp17氨基酸序列构建的系统进化分析表明,曼氏无针乌贼和加州双斑蛸(Octopus bimaculoides)进化关系最近。组织特异性分析发现Sp17在曼氏无针乌贼的生殖系统中均有较高的表达量,其中在精巢和储精囊中有显著表达。Sp17基因的成功克隆以及组织表达特异性分析对于深入研究其细胞定位以及生物学功能具有重要意义。

关键词：曼氏无针乌贼 Sp17 cDNA 生物信息学 real-time PCR

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