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Retrofitting low-performance units to abate sewer overflow pollution based on mathematical model and Sobol algorithm

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Journal of Hydrodynamics 2023年第4期35卷 770-781页

作者： Zhi-chao Zhao xiao-min Huang Hai-long Yin Jun-bo wen xiao-wei Fu Changjiang Institute of Survey PlanningDesign and ResearchWuhan430010China Key Laboratory of Yangtze River Water Environment Ministry of EducationTongji UniversityShanghai200092China State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai200092China Hubei Provincial Engineering Research Center for Comprehensive Water Environment Treatment in the Yangtze River Basin Wuhan430010China Key Laboratory of Urban Water Supply Water Saving and Water Environment Governance in the Yangtze River Delta of Ministry of Water ResourcesShanghai200092China

Optimal retrofit of low-performance units(LPUs)is promising to abate overflow pollutant mass loading of sewer systems during *** study presents a combination of mathematical model and Sobol algorithm to help identify LPUs of sewer systems and design retrofitting ***,the solution to minimize the overflow pollutant mass loading from sewers systems can be efficiently *** developed method was demonstrated at a catchment served by one wastewater treatment plant in the Chaohu City,Anhui Province of China,with five pumping stations and a total sewer length of 58.3 *** the catchment,there are three rivers and a small lake to receive overflows from the sewer *** them,one river that was mostly polluted was selected as the object of overflow pollution abatement during wet weather *** identifying the LPUs of the sewer system and developing retrofitting strategies using Sobol sequence,the mitigation of overflow pollution during wet weather period was *** show that the mass loading of chemical oxygen demand(COD)discharged into the target river could be reduced by 40.6%,by implementing optimal retrofit strategy of LPUs,i.e.,increasing the conveyance capacities of two pumping stations by 2.5–3.2 times and augmenting the diameters of 12 sewers by 1.25–1.29 *** further coordinate the abatement of overflow pollution and retrofit investment,Sobol sensitivity analysis was conducted to screen the dominant LPUs to update the optimal retrofit *** applying the updated strategy,the overflow COD mass loading per overflow event was close to that of non-updated strategy,while the retrofitting length of sewers was reduced by 40%.Therefore,on the basis of the presented method,decision-makers can flexibly develop retrofitting strategies of sewer system to abate overflow pollution during wet weathers in a cost-effective way.

关键词： Urban sewer system low-performance units Sobol sensitivity analysis sewer overflow optimal retrofit strategy

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Observed Climatic Variations in the Growing Season of Field Crops in Northeast China from 1992 to 2012

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Journal of Integrative Agriculture 2014年第7期13卷 1451-1461页

作者： LIU Yang JIANG wen-lai xiao Bi-lin LEI bo Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Key Laboratory of Resources Remote Sensing and Digital Agriculture Ministry of Agriculture Department of Irrigation and Drainage China Institute of Water Resources and Hydropower Research

To determine the potential effects of climate change on crop phenological development and productivity, an integrated analysis was conducted based on the observed climatic and phenological records of Northeast China from 1992 to 2012. A set of quality assurance procedures, including repeated record checks, agro-meteorological station selection, internal consistency checks, temporal outlier checks, spatial outlier checks, and interpolation of missing data, were designed and applied to the phenology datasets of spring maize and paddy rice. Our results indicated that almost all phenological dates of spring maize and paddy rice became increasingly delayed from 1992 to 2012, The duration of the growing season was prolonged, particularly for the grain-filling stage （GS3）. The prolonged growing season was beneficial to productivity. For spring maize, the average precipitation during GS3 decreased at a rate of 27.46 mm/decade, and the annual accumulated temperature over 10℃ increased at a rate of 31.07℃/ decade. Farmers initiatively adjusted crop cultivars and selected drought-resistant crops to cope with the challenges of drought.

关键词： climate change quality assurance phenology growing season field crop

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阳离子PLGA纳米颗粒作为核酸疫苗递送载体的能力及其特性评价

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中国生物制品学杂志 2020年第3期33卷 254-259页

作者：杨蕴琦卢渊录罗新茹嘉喜赵翔孙世琪冉旭华闻晓波郭慧琛黑龙江八一农垦大学动物科技学院黑龙江大庆163319 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室OIE/国家口蹄疫参考实验室甘肃兰州730046 中国农业科学院农业环境与可持续发展研究所北京100081

目的对阳离子聚乳酸-羟基乙酸共聚物[poly(D,L-lactide-co-glycolide),PLGA]纳米颗粒作为核酸疫苗载体的能力及其特性进行评价。方法采用双重乳化法制备阳离子PLGA纳米颗粒,检测其粒径分布和表面电势,获得合成的最适方案。通过考察其形... 详细信息

目的对阳离子聚乳酸-羟基乙酸共聚物[poly(D,L-lactide-co-glycolide),PLGA]纳米颗粒作为核酸疫苗载体的能力及其特性进行评价。方法采用双重乳化法制备阳离子PLGA纳米颗粒,检测其粒径分布和表面电势,获得合成的最适方案。通过考察其形貌、吸附效率、抵抗核酸酶降解能力、细胞毒性、细胞摄取及转染真核表达质粒的能力,分析其作为核酸疫苗载体的适用性及可行性。结果由两种试剂修饰PLGA制备的纳米颗粒粒径分布、表面电势及分散性最佳;透射电镜及扫描电镜下观察粒径均一且呈规则球形;对质粒DNA的吸附效率可达65%;能够有效保护吸附于纳米颗粒的质粒DNA免受核酸酶降解;两种细胞系中的细胞毒性试验均显示细胞存活率高于80%;共聚焦显微镜下可观察到其能被细胞内化;间接免疫荧光试验结果表明,其能有效转染真核表达质粒并使其正确表达。结论成功制备了具有递送核酸疫苗能力的阳离子PLGA纳米颗粒,为核酸疫苗载体的发展奠定了理论基础。

关键词： PLGA 纳米颗粒递送系统核酸疫苗

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Equilibrium Structures and Isomerization Reactions of the Unsaturated Germylenoid H_2C=GeLiF

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Chinese Journal of Structural Chemistry 2007年第5期26卷 613-617页

作者： LI wen-Zuo CHENG Jian-bo bGONG Bao-An xiao Cui-Ping Science and Engineering College of Chemistry and Biology Yantai University Yantai 264005 China Key Laboratory for Supramolecular Structure and Materials of Ministry of Education Jilin University Changchun 130012 China

The unsaturated germylenoid H2C=GeLiF has been studied by using DFT method at the B3LYP/6-311＋G （d, p） level. Geometry optimization calculations indicate that H2C=GeLiF has three equilibrium configurations, in which the p-complex is the lowest in energy and the most stable structure. Two transition states for isomerization reactions of H2C=GeLiF are located and the energy barriers are calculated. For the most stable one, vibrational frequencies and infrared intensities have been predicted.

关键词： unsaturated germylenoid H2C=GeLiF DFT B3LYP isomerization

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新城疫病毒F和HN基因真核表达载体的构建

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黑龙江八一农垦大学学报 2007年第4期19卷 54-57页

作者：闻晓波宋佰芬姜海芳崔玉东黑龙江八一农垦大学动物科技学院大庆163319 黑龙江八一农垦大学生命科技学院

根据GenBank中新城疫病毒(NDV)F、HN基因序列和真核细胞表达载体pEGPF-N1的克隆位点,分别设计一对引物,通过PCR方法获得了F、HN基因,经回收纯化,将产物连接到克隆载体pMD18-T上。酶切鉴定和序列分析后,再将目的基因亚克隆到真核细胞表... 详细信息

根据GenBank中新城疫病毒(NDV)F、HN基因序列和真核细胞表达载体pEGPF-N1的克隆位点,分别设计一对引物,通过PCR方法获得了F、HN基因,经回收纯化,将产物连接到克隆载体pMD18-T上。酶切鉴定和序列分析后,再将目的基因亚克隆到真核细胞表达载体pEGPF-N1上,经鉴定,成功构建含有NDVF和HN基因的真核细胞表达载体pEGPF-N1-F和pEGPF-N1-HN,为下一步研究NDVF和HN蛋白在细胞凋亡中的生物学功能奠定基础。

关键词：新城疫病毒 F基因 HN基因真核细胞表达载体

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PRRSV核衣壳蛋白(N蛋白)的克隆及其原核表达

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生物技术 2008年第1期18卷 18-21页

作者：赵喜红闻晓波冉旭华周恩民黑龙江八一农垦大学动物科技学院黑龙江大庆163319

目的:采用基因工程手段表达PRRSV VR 2332菌株的核衣壳蛋白(N蛋白)。方法:利用RT-PCR方法扩增出猪繁殖与呼吸综合征病毒(PRRSV)VR 2332菌株的核衣壳蛋白(N蛋白)基因并将其克隆到原核表达载pET30a(+)上,得到重组质粒rpET30a-PRRSV/N,并... 详细信息

目的:采用基因工程手段表达PRRSV VR 2332菌株的核衣壳蛋白(N蛋白)。方法:利用RT-PCR方法扩增出猪繁殖与呼吸综合征病毒(PRRSV)VR 2332菌株的核衣壳蛋白(N蛋白)基因并将其克隆到原核表达载pET30a(+)上,得到重组质粒rpET30a-PRRSV/N,并将其转入受体菌***21(DE3)pLysS感受态细胞中,经IPTG诱导,通过SDS-PAGE电泳检测和Western blotting分析表达产物的特性。结果:SDS-PAGE电泳检测和Western blotting分析结果表明,表达的蛋白大小约为19kDa,符合预期结果,并能与抗PRRSV N蛋白的单克隆抗体发生特异性反应,说明表达的蛋白具有良好的免疫原性,可应用于临床PRRSV抗体的检测。结论:在大肠杆菌表达系统内成功表达了PRRSV核衣壳蛋白(N蛋白)。

关键词：猪繁殖与呼吸综合征病毒(PRRSV) 核衣壳蛋白原核表达 Western blotting

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新城疫病毒F和HN蛋白的原核表达

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生物技术 2008年第3期18卷 11-13页

作者：冉旭华闻晓波赵喜红刘长凤黑龙江八一农垦大学动物科技学院黑龙江大庆163319

目的:高水平表达新城疫病毒(Newcastle Disease Virus,NDV)标准强毒F48E8株的F基因和HN基因。方法:利用PCR方法扩增出新城疫病毒(Newcastle Disease Virus,NDV)标准强毒F48E8株的去掉N端信号肽和C端跨膜区的F基因和HN基因并将其克隆到... 详细信息

目的:高水平表达新城疫病毒(Newcastle Disease Virus,NDV)标准强毒F48E8株的F基因和HN基因。方法:利用PCR方法扩增出新城疫病毒(Newcastle Disease Virus,NDV)标准强毒F48E8株的去掉N端信号肽和C端跨膜区的F基因和HN基因并将其克隆到原核表达载pET30a上,得到重组质粒rpET30a-NDV/F和rpET30a-NDV/HN,转化到受体菌Rosetta-gamiTM2感受态细胞中,经IPTG诱导表达,SDS-PAGE电泳和Western blot检测。结果:表达的F蛋白大小约为40kD,HN蛋白大小约为51kD左右,符合预期结果。Western blot分析表明,能与抗His-tag单克隆抗体发生特异性反应。结论:重组蛋白在原核表达系统内得到了高水平的表达。说明了去除F和HN基因N端信号肽和C端跨膜区的必要性。

关键词：新城疫病毒(NDV) F蛋白 HN蛋白原核表达

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Natural products as potent inhibitors of hypoxia-inducible factor-1α in cancer therapy

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Chinese Journal of Natural Medicines 2020年第9期18卷 696-703页

作者： ZHONG Jin-Cheng LI xiao-bo LYU wen-Yu YE wen-Cai ZHANG Dong-Mei College of Pharmacy Jinan UniversinyGuangzhou 510632.China Guangdong Province Key Laboratory of Pharmacodynamic Constituents 0f Traditional Chinese Medicine and New Drugs Research Jinan UniversityGuangzhou 510632China

Hypoxia is a prominent feature of tumors. Hypoxia-inducible factor-1α(HIF-1α), a major subunit of HIF-1, is overexpressed in hypoxic tumor tissues and activates the transcription of many oncogenes. Accumulating evidence has demonstrated that HIF-1α promotes tumor angiogenesis, metastasis, metabolism, and immune evasion. Natural products are an important source of antitumor drugs and numerous studies have highlighted the crucial role of these agents in modulating HIF-1α. The present review describes the role of HIF-1α in tumor progression, summarizes natural products used as HIF-1α inhibitors, and discusses the potential of developing natural products as HIF-1α inhibitors for the treatment of cancer.

关键词： Hypoxia-inducible factor-1α Hypoxia Cancer Natural products Anticancer treatment

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猪圆环病毒2型ORF3基因的原核表达

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中国畜牧兽医 2010年第6期37卷 171-174页

作者：冉旭华李树东闻晓波王密杨焕民倪宏波侯喜林朴范泽邵磊李晓娟李冬野周恩民黑龙江八一农垦大学动物科技学院预防兽医学省重点实验室大庆163319

用原核表达系统表达猪圆环病毒2型ORF3基因,分析ORF3蛋白的抗原性。根据GenBank上公布的猪圆环病毒2型(PCV-2)核苷酸序列进行分析,针对ORF3基因设计并合成1对特异性引物,PCR扩增该基因,并将此片段克隆入原核表达载体pET-32a(+)上,命名为... 详细信息

用原核表达系统表达猪圆环病毒2型ORF3基因,分析ORF3蛋白的抗原性。根据GenBank上公布的猪圆环病毒2型(PCV-2)核苷酸序列进行分析,针对ORF3基因设计并合成1对特异性引物,PCR扩增该基因,并将此片段克隆入原核表达载体pET-32a(+)上,命名为pET32a-ORF3,转化大肠杆菌Rosetta-blue(DE3)pLacⅠ感受态细胞,1.0mmol/L IPTG37℃诱导表达。结果表明,PCR扩增得到315bp的片段,重组蛋白大小约为29ku,与预期大小相符。Western blotting分析结果表明,重组蛋白能与抗His-tag的单克隆抗体反应,不与PCV-2阳性血清发生反应。猪圆环病毒2型ORF3基因能在大肠杆菌中成功表达,但与阳性血清之间没有反应原性,为进一步研究PCV-2ORF3蛋白的功能及特性奠定基础。

关键词：猪圆环病毒2型 ORF3基因原核表达

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Distribution of ^(56)Ni Yields of Type Ia Supernovae and its Implication for Progenitors

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Chinese Journal of Astronomy and Astrophysics 2008年第1期8卷 71-80页

作者： bo Wang Xiang-Cun Meng xiao-Feng Wang Zhan-wen Han National Astronomical Observatories / Yunnan Observatory Chinese Academy of Sciences Kunming 650011 Graduate School of Chinese Academy of Sciences Beijing 100049 Tsinghua Center for Astrophysics （THCA） and Department of Physics Tsinghua University Beijing 100084 Astronomy Department University of California at Berkeley CA 94720 USA

The amount of 56↑Ni produced in Type Ia supernova （SN Ia） explosion is probably the most important physical parameter underlying the observed correlation of SN Ia luminosities with their light curves. Based on an empirical relation between the 56↑Ni mass and the light curve parameter △m15, we obtained rough estimates of the 56↑Ni mass for a large sample of nearby SNe Ia with the aim of exploring the diversity in SN Ia. We found that the derived 56↑Ni masses for different SNe Ia could vary by a factor of ten （e.g., MNi = 0.1 - 1.3 M⊙）, which cannot be explained in terms of the standard Chandrasekhar-mass model （with a 56↑Ni mass production of 0.4 - 0.8 M⊙）. Different explosion and/or progenitor models are clearly required for various SNe Ia, in particular, for those extremely nickel-poor and nickel-rich producers. The nickel-rich （with MNi 〉 0.8 M⊙） SNe Ia are very luminous and may have massive progenitors exceeding the Chandrasekhar-mass limit since extra progenitor fuel is required to produce more 56↑Ni to power the light curve. This is also consistent with the finding that the intrinsically bright SNe Ia prefer to occur in stellar environments of young and massive stars. For example, 75% SNe Ia in spirals have △ml5 〈 1.2 while this ratio is only 18% in E/S0 galaxies. The nickel-poor SNe Ia （with MNi 〈 0.2 M⊙） may invoke the sub- Chandrasekhar model, as most of them were found in early-type E/S0 galaxies dominated by the older and low-mass stellar populations. This indicates that SNe Ia in spiral and E/S0 galaxies have progenitors of different properties.

关键词： stars evolution - supernovae general - white dwarfs

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