检索结果-南通市图书馆

Effect of recombinant human platelet-derived growth factor B on cat corneal endothelial cell viability mediated by adeno-associated virus

引用

International Journal of Ophthalmology(English edition) 2012年第4期5卷 419-423页

作者： wen-Juan Luo, Hui Li Department of Ophthalmology the Affiliated Hospital of Medical College Qingdao University Qingdao 266003 Shandong Province China Department of Pathology the Affiliated Hospital of Medical College Qingdao University Qingdao 266003 Shandong Province China Central Laboratory of the Affiliated Hospital of Medical College Qingdao University Qingdao 266003 Shandong Province China

AIM: To transduce recombinant human platelet-derived growth factor B (PDGF-B) gene adeno-associated virus(AAV) to in vitro cultured cat corneal endothelial cell (CEC) and observe the effect of the expressed PDGF-BB protein on the viability of cat CEC. METHODS: Cat cornea endothelium was torn under microscope and rapidly cultivated in DMEM to form single layer CEC and the passage 2 endothelial cells were used in this study. The recombinant human PDGF-B gene AAV was constructed and transduced into cat CEC directly. Three groups were as following: blank control group, AAV control group and recombinant AAV group. At 24 hours, 48 hours, and 5 days after transduction, total RNA was extracted from the CEC by Trizol and the expression of PDGF-B gene was detected by fluorescence quantitative polymerase chain reaction. Viability of the transduced CEC was detected at 48 hours after transduction by MTT assay. Cell morphology was observed under inverted phase contrast microscope. RESULTS: With the torn endothelium culture technique, we rapidly got single layer cat CEC. At 24 hours, 48 hours and 5 days after transduction, fluorescence quantitative polymerase chain reaction showed there was no significant difference of the expressed PDGF-B gene mRNA between blank control group and AAV control group (P>0.05). In contrast, there were significant differences between two control groups and recombinant AAV group (P<0.05). MTT assay showed that in recombinant AAV group, the expressed PDGF-BB protein could promote the viability of cat CEC. Morphology observation showed at 48 hours after transduction, cells in CEC-AAV-PDGF-B group proliferated into bigger scales in regular triangle to hexagon shape with distinct boundary, while the number of cells was significantly less in the two control groups. CONCLUSION: The recombinant AAV-PDGF-B expresses biological active PDGF-BB protein in cat CEC, which promotes the viability and proliferation of cells.

关键词： platelet-derived growth factor corneal endothelial cell transduction viability proliferation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Relationship between CYP1A1 polymorphisms and invasion and metastasis of breast cancer

引用

Asian Pacific Journal of Tropical Medicine 2013年第10期6卷 835-838页

作者： Hua Wang wen-Jian Wang Laboratory of Department of Surgery First Affiliated HospitalSun Yat-sen University

Objective:To investigate the relationship between CYPIA1 genetic polymorphisms and the invasion and metastasis of breast ***:The CYP1A1 gene polymorphism(an T-C transversion at nucleotide position 3801)was detected by the polymerase chain reaction and restriction fragment length polymorphism in 80 cases with breast cancer and 60 samples of normal breast *** difference in genotypic distribution frequency between the groups,the correlation between the genotypes and the factors related to prognosis were ***:The incidence of homozygous and variant genotypes had no difference between the breast cancer group and controls group(P=0.746).The proportion of variant genotype increased as clinical stage(P=0.006)advanced,as well as with increased numbers of lymph node metastases(P=0.010).Conclusions:In patients with breast cancer there is a correlation between the CYP1A1 CC allele and some factors indicating poor prognosis,including more lymph node metastases as well as a more advanced clinical stage.

关键词： Breast cancer CytochromeP450 CYP1Al Gene polymorphism Invasion Metastasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Combustion Wave Propagation of a Modular Porous Burner with Annular Heat Recirculation

引用

Journal of Thermal Science 2020年第1期29卷 98-107页

作者： SONG Fuqiang wen Zhi FANG Yuan WANG Enyu LIU Xunliang School of Energy and Environmental Engineering University of Science and Technology BeijingBeijing 100083China School of Energy and Environmental Engineering Hebei University of TechnologyTianjin 300401China

A numerical investigation of the different arrangements of porous media in a combustor with annular heat recirculation is *** effect of annular heat recirculation and porous block arrangement on the characteristics of combustion wave propagation is numerically *** show that power input,heat capacity of porous matrix,arrangement of porous blocks,and annular heat recirculation are major factors that influence the propagation of combustion *** overall temperature of ceramic porous burner is higher than that of ceramic-metal type burner due to the lower heat storage capacity of the former,especially for the temperature *** flame temperature is higher upstream and lower downstream with metal foams in the annulus than that without metal *** flame temperature of uniformity type burner is more uniform than that of gradually-varied and modular type *** flame front moves more slowly with metal foams in the annulus than that without metal foams due to the better preheating effect of metal *** flame position moves downstream,and the flame temperature gradually decreases and is eventually extinguished due to the low preheating temperature.

关键词： flashback start-up modular porous burner heat recirculation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Choice for graphene as conductive additive for cathode of lithium-ion batteries

引用

Journal of Energy Chemistry 2019年第3期28卷 19-26页

作者： Ying Shi Lei wen Songfeng Pei Minjie Wu Feng Li Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences

Graphene is a promising conductive additive for the lithium-ion batteries(LIBs) and shows great potential especially with its fast development of the large scale fabrication technology. This work has explored the influence of the incorporation of graphenes prepared by three typical methods on the electrochemical performance of the LiCoO_2-based cathode focusing on the choice for the effective graphene as conductive additive for the cathode of LIBs. Through the comparison of the intrinsic characteristics of graphenes and the electrochemical performance of electrodes with graphene, it is found that graphene with low disorder degree and large size is not suitable for LiCoO_2 cathodes as conductive additive. Conversely, the graphene with oxygen functional groups, relatively low surface area and proper size displays much better electrochemical performance when it is used as conductive additive. This work also demonstrates the transmission mechanism for different graphenes as conductive additives in the LiCoO_2 materials, and further reveals that the conductivity of graphene is not the only factor as conductive additives, surface chemistry and sheet size of the graphene are also essential factors which greatly influence the electrochemical performance of electrode. In addition, when combined with Super P, only 1% graphene is enough to construct an efficient conductive network in the electrode. This study also gives a new sight on the practical application of graphene as conductive additive for high performance LIBs.

关键词： Graphene Conductive additive Lithium-ion batteries LiCoO2 cathode

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Omega-Liutex method for the interaction among wave, current and fixed vertical cylinder

引用

Journal of Hydrodynamics 2023年第1期35卷 83-94页

作者： Bo Han Zhi Pan Wei-wen Zhao De-cheng Wan Computational Marine Hydrodynamics Lab(CMHL) School of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Wuhan Second Ship Design and Research Institute Wuhan430205China

This paper presents a numerical investigation of the interaction among wave,flow and a fixed cylinder using the naoe-FOAM-SJTU solver,which is a viscous solver for hydrodynamics based on *** simulation considers the strong nonlinear interaction among a regular wave,flow,and a single fixed cylinder due to viscosity,which is common in offshore *** study focus on the vortex induced by viscosity and its significant role in hydrodynamic performance computation.A mesh convergence study is conducted prior to the simulation,and a proper set of mesh is *** simultaneous generation of the regular wave and flow is realized using the numerical field boundary,and the wave propagation is *** results of wave elevation and pressures captured by wave gauges and probes around the fixed cylinder are compared with experiment *** structure of vortices is displayed at significant times,and the phenomena of wave elevation and pressure changes around the cylinder are analyzed in detail using the Omega-Liutex identification method.

关键词： Wave-current interaction vortex Omega-Liutex method naoe-FOAM-SJTU solver numerical simulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regulation of radicals by hydrogen-donor solvent in direct coal liquefaction

引用

Frontiers of Chemical Science and Engineering 2022年第12期16卷 1689-1699页

作者： Wang Li wen-Ying Li Xing-Bao Wang Jie Feng State Key Laboratory of Clean and Efficient Coal Utilization Taiyuan University of TechnologyTaiyuan 030024China

Radicals are important intermediates in direct coal *** radicals can cause the cleavage of chemical *** high temperatures,radical fragments can be produced by the splitting of large organic molecules,which can break strong chemical bonds through the induction pyrolysis of *** reaction between the formation and annihilation of coal radical fragments and the effect of hydrogen-donor solvents on the radical fragments are discussed in lignite *** the hydroxyl and ether bonds as indicators,the effects of different radicals on the cleavage of chemical bond were investigated employing density functional theory calculations and lignite hydrogenolysis *** showed that the adjustment of the coal radical fragments could be made by the addition of hydrogendonor *** showed that the transition from coal radical fragment to H radical leads to the variation of product *** synergistic mechanism of hydrogen supply and hydrogenolysis of hydrogen-donor solvent was proposed.

关键词： direct coal liquefaction hydrogen-donor solvent induced pyrolysis radical mechanism density functional theory calculations

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recurrent testicular undifferentiated pleomorphic sarcoma

引用

Asian Journal of Andrology 2023年第4期25卷 541-543页

作者： Jun-Ping Han wen-Xiao Luo Jun-wen Zeng wen-Xia Ma Qiang Zhao Yong-Qiang Xie Zheng-Chao Zhang Department of Pathology The Third Affiliated Hospital of Gansu University of Traditional Chinese MedicineBaiyin 730900China Department of Urology The Third Affiliated Hospital of Gansu University of Traditional Chinese MedicineBaiyin 730900China Department of General Surgery The Second Hospital of Lanzhou UniversityLanzhou 730000China

Dear Editor,Undifferentiated pleomorphic sarcoma(UPS)is a subtype of soft tissue sarcoma first reported by O’Brien and Stout in 1964.1 It is a common soft tissue sarcoma in adults,commonly occurring in the extremities and retroperitoneum.2,3 However,the incidence of testicular UPS is extremely rare in China and the rest of the *** addition,radical orchiectomy is the preferred treatment for this disease,but the role of postoperative radiotherapy and chemotherapy remains *** paper reports the diagnosis,treatment process,and prognosis of a case of recurrent testicular *** performing a literature review,it was found that postoperative radiotherapy and chemotherapy may prolong the progression-free survival of the patient,so we present this paper to raise clinicians’understanding of the *** study was approved by the Ethics Committee of The Third Affiliated Hospital of Gansu University of Traditional Chinese Medicine(Baiyin,China;approval ***-X2-2020-018).The patient was informed of the content and significance of the study and signed an informed consent form.

关键词： sarcoma diagnosis approval

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Reversible isomerization of donor-acceptor Stenhouse adduct derivatives in water through dendritic confinement

引用

Science China Chemistry 2024年第5期67卷 1636-1646页

作者： Jiaxing Zhang Qingyun Zhao Xinyan Su Afang Zhang wen Li International Joint Laboratory of Biomimetic and Smart Polymers School of Materials Science and EngineeringShanghai UniversityShanghai 200444China

Modulating reversible isomerization of hydrophobic dyes in aqueous solutions is greatly desired. Here we report on reversible isomerization of solvatochromic donor-acceptor Stenhouse adducts(DASAs) in water through the confinement from dendritic oligoethylene glycols(OEGs). Dendronization of DASAs with dendritic OEGs affords them characteristic thermoresponsiveness. These dendronized DASAs spontaneously isomerize in water from hydrophobic linear state into hydrophilic cyclic state at room temperature due to the strong hydration. However, hydrophobic microenvironment through thermally dehydration and collapse of the dendritic OEGs at elevated temperatures confines hydration of the DASA moieties and mediates their interactions with the collapsed hydrophobic OEG domains, affording their isomerization recovery in water efficiently from the hydrophilic cyclic state into the hydrophobic linear state. The confinement-mediated reversible isomerization of DASA moieties in water can be repeated through alternative photo-irradiation and thermal dehydrations, exhibiting excellent fatigue resistance.

关键词： dendrimers donor-acceptor Stenhouse adducts isomerization confinement thermoresponsiveness

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-temperature friction behavior of amorphous carbon coating in glass molding process

引用

Friction 2021年第6期9卷 1648-1659页

作者： kangsen LI Gang XU Xiaobin wen Jun ZHOU Feng GONG Guangdong Provincial Key Laboratory of Micro/Nano Optomechatronics Engineering College of Mechatronics and Control EngineeringShenzhen UniversityShenzhen 518060China Shenzhen Kingmag Precision Techonology Co.LTD. Shenzhen 518172China

In the glass molding process,the sticking reaction and fatigue wear between the glass and mold hinder the service life and functional application of the mold at the elevated *** improve the chemical inertness and anti-friction properties of the mold,an amorphous carbon coating was synthesized on the tungsten carbide-cobalt(WC–8Co)substrate by magnetron *** friction behavior between the glass and carbon coating has a significant influence on the functional protection and service life of the ***,the glass ring compression tests were conducted to measure the friction coefficient and friction force of the contact interface between the glass and amorphous carbon coating at the high ***,the detailed characterization of the amorphous carbon coating was performed to study the microstructure evolution and surface topography of the amorphous carbon coating during glass molding process by scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),Ramon spectroscopy,and atomic force microscope(AFM).The results showed that the amorphous carbon coating exhibited excellent thermal stability,but weak shear friction *** friction coefficient between the glass and coating depended on the ***,the service life of the coating was governed by the friction force of the contact interface,processing conditions,and composition *** work provides a better understanding of the application of carbon coatings in the glass molding.

关键词： glass molding process high temperature friction behavior amorphous carbon coating

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Silk-derived peptide nanospirals assembled by self-propelled wormlike filaments

引用

Nano Research 2023年第1期16卷 1414-1420页

作者： Huijuan wen Jinrong Yao Xin Chen Shengjie Ling Zhengzhong Shao State Key Laboratory of Molecular Engineering of Polymers Advanced Material LaboratoryDepartment of Macromolecular ScienceFudan UniversityShanghai 200433China School of Physical Science and Technology ShanghaiTech University393 Middle Huaxia RoadShanghai 201210China

Swirl-like nanospiral is a common structure found in free-swimming biological systems,such as microtubules and actin filaments or slender *** is desired for artificially designed dynamic ***,the spiral formation has rarely been reported in both engineered peptides and regenerated ***,we report that such a unique assembly behavior can be achieved by using a fusion peptide consisting of a silk-derived peptide(i.e.,GAGAGAGY)and a hydrophobic,photoresponsive azobenzene(Azo)*** this fusion structure,GAGAGAGY acts as a domain that spontaneously forms an elongated filament in an aqueous solution,while Azo acts as a"light-operated switch"that can undergo photoinduced isomerization to modulate the self-propulsion forces and assembly *** this design,the critical factors that affect the assembly of Azo-GAGAGAGY filament,including(i)length and flexibility of filaments;(ii)propulsion,and(iii)excluded volume interactions force the tip of the filament to wind up,can be regulated to realize the spiral *** addition,the configurations of Azo-GAGAGAGY filaments,such as straight nanoribbons,wavy nanoribbons,single-circle spiral,and multiple-circle spiral,can be facilely mediated by changing the preparation procedure,concentration,and pH value of Azo-GAGAGAGY solution,as these changes have significant influences on self-propulsion *** findings can help in the better understanding of nonequilibrium thermodynamics and collective behavior of biological *** findings can be used as a guideline for the designs of nanoactuators,microswimmers,transformable microrobots,and intelligent drug carriers.

关键词： nanospiral silk-derived peptide self-assembly worm-like filaments photoinduced isomerization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：