检索结果-南通市图书馆

Sustained-release genistein from nanostructured lipid carrier suppresses human lens epithelial cell growth

International Journal of Ophthalmology(English edition) 2016年第5期9卷 643-649页

作者： jin-Lu Liu wen-ji Zhang Xue-Dong Li Na Yang Wei-San Pan Jun Kong jin-Song Zhang Department of Ophthalmology the Fourth Affiliated Hospital of China Medical UniversityEye Hospital of China Medical UniversityKey Lens Research Laboratory of Liaoning Province Department of Pharmaceutics School of PharmacyShenyang Pharmaceutical University

AIM： To design and investigate the efficacy of a modified nanostructured lipid carrier loaded with genistein（Gen-NLC） to inhibit human lens epithelial cells（HLECs） proliferation.·METHODS： Gen-NLC was made by melt emulsification method. The morphology, particle size（PS）, zeta potentials（ZP）, encapsulation efficiency（EE） and in vitro release were characterized. The inhibition effect of nanostructured lipid carrier（NLC）, genistein（Gen） and Gen-NLC on HLECs proliferation was evaluated by cell counting kit-8（CCK-8） assay, gene and protein expression of the proliferation marker Ki67 were evaluated with real-time quantitative polymerase chain reaction（RT-q PCR） and immunofluorescence analyses.·RESULTS： The mean PS of Gen-NLC was 80.12±1.55 nm with a mean polydispersity index of 0.11±0.02. The mean ZP was-7.14 ±0.38 m V and the EE of Gen in the nanoparticles was 92.3% ±0.73%. Transmission electron microscopy showed that Gen-NLC displayed spherical-shaped particles covered by an outer-layer structure. In vitro release experiments demonstrated a prolonged drug release for 72 h. The CCK-8 assay results showed the NLC had no inhibitory effect on HLECs and Gen-NLC displayed a much more prominent inhibitory effect on cellular growth compared to Gen of the same concentration. The m RNA and protein expression of Ki67 in LECs decreased significantly in Gen-NLC group.·CONCLUSION： Sustained drug release by Gen-NLCs may impede HLEC growth.

关键词： posterior capsular opacification genistein nanostructured lipid carrier human lens epithelial cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Asian Games:How Much Gold is in the Gold Medals?

引用

Women of China 1999年第4期 15-18页

作者： wen ji LIU XIAOHONG

DESPITE China’s glorious victoryin Bangkok at the last AsianGames of this century,in whichthe Chinese team topped the list with129 gold medals,the Chinese athletesare asked to keep their fingers crossedfor the Olympic *** a briefrest,the Chinese athletes have returnedto their training ground in preparationfor the fiercer competition at the

关键词： As The Asian Games:How Much Gold is in the Gold Medals

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Variable phenotypes and outcomes associated with the MMACHC c.482G>A mutation:follow-up in a large CblC disease cohort

引用

World Journal of Pediatrics 2024年第8期20卷 848-858页

作者： Sheng-Nan Wu Hui-Shu E Yue Yu Shi-Ying Ling Li-Li Liang wen-Juan Qiu Hui-wen Zhang Rui-Xue Shuai Hai-Yan Wei Chi-Ju Yang Peng Xu Xi-Gui Chen Hui Zou ji-Zhen Feng Ting-Ting Niu Hai-Li Hu Kai-Chuang Zhang De-Yun Lu Zhu-wen Gong Xia Zhan wen-Jun ji Xue-Fan Gu Yong-Xing Chen Lian-Shu Han Department of Endocrinology and Metabolism Henan Key Laboratory of Children’s Genetics and Metabolic DiseasesChildren’s Hospital Affiliated to Zhengzhou UniversityHenan Children’s HospitalZhengzhou Children’s HospitalNo.255 Gangdu StreetZhengzhouChina Department of Pediatric Endocrinology and Genetics Fujian Children’s HospitalFuzhouChina The Center for Pediatric Liver Diseases Children’s HospitalFudan UniversityShanghaiChina Department of Pediatric Endocrinology/Genetics Shanghai Institute for Pediatric ResearchXinhua HospitalSchool of MedicineShanghai Jiao Tong UniversityNo.1665 Kongjiang RoadShanghaiChina Department of Pediatrics Second Affiliated Hospital of Naval Medical UniversityShanghaiChina Center of Neonatal Disease Screening Jining Maternal and Child Health Care HospitalJiningChina Center of Neonatal Disease Screening Jinan Maternal and Child Health Care HospitalJinanChina Center of Neonatal Disease Screening Shijiazhuang Maternal and Child Health Care HospitalShijiazhuangChina Center of Neonatal Disease Screening Shandong Maternal and Child Health Care HospitalJinanChina Center of Neonatal Disease Screening Hefei Maternal and Child Health Care HospitalHefeiChina

Background The aim of this study was to characterize the variable phenotypes and outcomes associated with the methylmalonic aciduria and homocystinuria type C protein gene(MMACHC)c.482G>A mutation in 195 Chinese cases with CblC *** We carried out a national,retrospective multicenter study of 195 Chinese patients with CblC disease attributable to the MMACHC c.482G>A variant either in a homozygous or compound heterozygous *** control group consisted of 200 patients diagnosed with CblC disease who did not possess the c.482G>A *** features,including disease onset,symptoms,biochemical metabolites,gene mutation,and follow-up outcomes were reviewed and analyzed in *** median follow-up period spanned 3 years and 8 months,with a range of 1 year and 2 months to 12 years and 10 *** Among 195 patients carrying the c.482G>A variant,125(64.1%)cases were diagnosed by newborn screening(NBS),60(30.8%)cases were detected due to disease onset,and 10(5.1%)cases were identified from sibling *** hundred and seventeen(93.6%)individuals who were diagnosed by NBS,and nine patients who came from sibling diagnoses remained asymptomatic in this *** 69 symptomatic patients of the c.482G>A group,more patients presented with later onset,and the top six common clinical symptoms at disease onset were developmental delay(59.4%),lower limb weakness and poor exercise tolerance(50.7%),cognitive decline(37.7%),gait instability and abnormal posture(36.2%),seizures(26.1%),and psychiatric and behavioral disturbances(24.6%).In the 159 symptomatic patients lacking c.482G>A variants,the most frequently observed clinical manifestations at disease onset included developmental delay(81.8%),lethargy and feeding difficulty(62.9%),lower limb weakness and poor exercise tolerance(54.7%),prolonged neonatal jaundice(51.6%),vomiting(47.2%),and seizures(32.7%).Before treatment,the levels of blood propionylcarnitine,propionylcarnitine/acetylcarnitine ratio

关键词： c.482G>A CblC disease MMACHC gene Newborn screening Outcome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

People Engaged in the Course of Charity

引用

Women of China 1999年第2期 20-22页

作者： wen ji PAN ZHONGMING

THE action of the ShanghaiExpatriate Wives CharityAssociation (SEA) is touching. Themembers of the association cometogether to do works of charity.

关键词： Zhang In People Engaged in the Course of Charity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Indicators of prognosis after liver transplantation in Chinese hepatocellular carcinoma patients

引用

World Journal of Gastroenterology 2009年第33期15卷 4170-4176页

作者： jin Li Lu-Nan Yan Jan Yang Zhe-Yu Chen Bo Li Yong Zeng Tian-Fu wen ji-Chun Zhao wen-Tao Wang jia-Yin Yang Ming-Qing Xu Yu-Kui Ma Division of Liver Transplantation West-China Hospital West China Medical School of Sichuan University Chengdu 610041 Sichuan Province China

AIM： To identify prognostic factors of patients with hepatocellular carcinoma （HCC）, who were treated by orthotopic liver transplantation （OLT）. METHODS： From January 2000 to October 2006, 165 patients with HCC underwent OLT. Various clinicopathological risk factors for actuarial and recurrencefree survival were identified using the Kaplan-Meier method with the log-rank test. The Cox proportional hazards model was used to identify independently predictive factors for actuarial and recurrence-free survival, which were used to propose new selection criteria. We compared the outcome of the subgroup patients meeting different criteria. Survival analysis was performed using the Kaplan-Meier method with the log-rank test. RESULTS： The median follow-up was 13.0 mo （2.8-69.5 too）. Overall, 1-, 2-, 3- and 5-year actuarial survival was 73.3%, 45.6%, 35.4% and 32.1%, respectively. One-, 2-, 3- and 5-year overall recurrencefree survival was 67.0%, 44.3%, 34.5% and 34.5%, respectively. In univariate analysis, number of tumors, total tumor size, lobar distribution, differentiation, macrovascular invasion, microvascular invasion, capsulation of the tumor, and lymph node metastasis were found to be associated significantly with actuarial and tumor-free survival. By means of using the multivariate Cox proportional hazards model, total tumor size and macrovascular invasion were found to be independent predictors of actuarial and tumor-free survival. When the selection criteria were expanded into the proposed criteria, there was no significant difference in 1-, 2-, 3- and 5-year actuarial and tumor-free survival of the 49 patients who met the proposed criteria （97.6%, 82.8%, 82.8% and 82.8%, and 90.7%, 82.8%, 68.8% and 68.8%, respectively） compared with that of patients who met the Milan or University of California, San Francisco （UCSF） criteria. CONCLUSION： Macrovascular invasion and total tumor diameter are the strongest prognostic factors. The proposed criteria do not adversely aff

关键词： Hepatocellular carcinoma Prognosis Liver transplantation Outcome assessment Survival

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic sequencing of a patient with Kallmann syndrome plus 5α-reductase type 2 deficiency

引用

Asian Journal of Andrology 2017年第3期19卷 386-387页

作者： wen ji Lu-Yao Zhang Fu-Cheng Li Yu Wang Wei He Qi-Qi Yin Zhi-Hong Liao Department of Endocrinology 1st Affiliated Hospital of Sun Yat-sen University Guangzhou 510080 China Department of Medical Genetics Genome Research Center Zhongshan School of Medicine Sun Yabsen University Guangzhou 510080 China.

Dear Editor, Kallmann syndrome （KS） is a phenotypically and genetically heterogeneous disorder featured by hypogonadotropic hypogonadism and congenital hyposmia or anosmia, accompanined with renal dysplasia, hearing loss, and craniofacial defects sometimes. More than 20 genes have been identified causing KS either alone or in combination.

关键词： @@

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

第二深度空间(5000～10000m)油、气形成与聚集的深层物理与动力学响应

引用

地球物理学报 2013年第12期56卷 4164-4188页

作者：滕吉文杨辉中国科学院地质与地球物理研究所北京100029

在当今全球各国对油、气能源的需求和我国在工业化进程中油气能源十分紧缺的前提下，共享世界油、气能源的同时，必须立足于本土，迅速建立起安全、稳定，且可保证持续供给的油、气能源战略后备基地．油、气田勘探和开采中，尚存在着大... 详细信息

在当今全球各国对油、气能源的需求和我国在工业化进程中油气能源十分紧缺的前提下，共享世界油、气能源的同时，必须立足于本土，迅速建立起安全、稳定，且可保证持续供给的油、气能源战略后备基地．油、气田勘探和开采中，尚存在着大于或远大于通常所规范的油、气生成稳定温度和深度限定，而向深部勘察油、气的理念又受到传统油、气成因理论的严峻挑战．为此，近些年来，通过对沉积建造、结晶基底，深部油气生成的物理（主指温度、压力）条件、物源和聚集储存空间的研究，提出：①沉积盆地中存在双相（陆相＋海相）沉积建造，而基底则分为中、新生代沉积基底和古老的变质岩结晶基底，即双层基底；②地壳深部介质的物理一化学属性和变质岩及存储空间为油、气的生成和聚集提供了物质基础；③厘定了在有机成因主导下，油、气混合成因（有机＋无机）的新理念；④第二深度卒间（5000-10000m）的油、气探杏和开发将必是未来深部发现大型和超大型油、气田和深化研究的必然轨迹．

关键词：双相沉积结晶基底深部温、压条件油、气混合成因第二深度空间

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

不确定跳变系统反馈预测控制

引用

控制与决策 2010年第6期25卷 916-920,933页

作者：闻继伟刘飞江南大学自动化研究所江苏无锡214122

针对一类受限不确定离散Markov跳变系统,提出一种反馈预测控制器设计方法.为便于工程应用,该方法考虑了各模态下的动态系统参数存在多胞不确定性,以及各模态间的跳变转移概率部分未知的情形.通过优化无穷时域的二次型性能指标来确定预... 详细信息

针对一类受限不确定离散Markov跳变系统,提出一种反馈预测控制器设计方法.为便于工程应用,该方法考虑了各模态下的动态系统参数存在多胞不确定性,以及各模态间的跳变转移概率部分未知的情形.通过优化无穷时域的二次型性能指标来确定预测控制器及其对应的椭圆不变集,控制器保证了闭环系统鲁棒均方稳定.同时,用所求得的控制增益在线构造了一组渐近稳定的多面体不变集,在一定程度上扩大了系统状态可行集的范围.数值示例验证了所提方法的可行性和有效性.

关键词： Markov跳变系统跳变转移概率反馈预测控制多面体不变集半正定规划线性规划

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transformation of Nitrogen State in High-Level N-Doped Gem-Quality Diamond Crystal Annealed at High Temperature and High Pressure

引用

Chinese Physics Letters 2012年第10期29卷 151-154页

作者： HUANG Guo-Feng jiA Xiao-Peng MA Hong-An BAI Hong-Bo YIN ji-wen HU Yi-Ga Department of Physics and Electronic Information Engineering Chifeng CollegeChifeng 024000 National Lab of Superhard Materials Jilin UniversityChangchun 130012

The colourless IaA-type gem-quality diamond crystals containing a high concentration of nitrogen(1500–1700 ppm)were successfully prepared by annealing the as-grown Ib-type N-doped diamonds at a high temperature and high pressure in China-type cubic anvil high-pressure *** were carried out at pressures of 6.5–7.0 GPa and temperatures from 1900 K to 2100 *** treatment on high-level N-doped diamond crystals shows that the colour of the diamond crystals is obviously reduced from green to colourless after annealing treatment within 1 h at a higher temperature,which is induced by nitrogen aggregation in the diamond lattice indicated by infrared(IR)*** is further revealed that active energy of the nitrogen atom transforming from the dispersed form to the aggregated form is much lower than that in the standard Ib-type diamond crystals with nitrogen concentration less than 300 *** colourless IaA-type diamond crystal prepared by annealing at 2100 K displays the same properties in IR spectra as the high-quality natural diamonds which are classified into the IaA type.

关键词： annealing treatment Transformation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaporation of saline colloidal droplet and deposition pattern

引用

Chinese Physics B 2020年第1期29卷 366-372页

作者： Hong-Hui Sun Wei-Bin Li wen-jie ji Guo-Liang Dai Yong Huan Yu-Ren Wang Ding Lan National Microgravity Laboratory Institute of MechanicsChinese Academy of SciencesBeijing 100190China State Key Laboratory of Nonlinear MechanicsLNM Institute of MechanicsChinese Academy of SciencesBeijing 100190China School of Engineering Sciences University of Chinese Academy of SciencesBeijing 100049 China

The dynamic process of the evaporation and the desiccation of sessile saline colloidal droplets,and their final deposition are *** the evaporation,the movement of the colloidal particles shows a strong dependence on the salt concentration and the droplet *** final deposition pattern indicates a weakened coffee-ring effect in this mixed droplet *** microscopic observation reveals that as evaporation proceeds,the particle motion trail is affected by the salt concentration of the droplet *** Marangoni flow,which is induced by surface tension gradient originating from the local evaporative peripheral salt enrichment,suppresses the compensation flow towards the contact line of the *** inhomogeneous density and concentration field induced by evaporation or crystallization can be the major reason for various *** last stage,the distribution and crystallization of Na Cl are affected by the colloidal particles during the drying of the residual liquid film.

关键词： saline droplet evaporation deposition pattern disease diagnosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：