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A human antibody potently neutralizes RSV by targeting the conserved hydrophobic region of prefusion F

2020中国汽车工程学会年会暨展览会

作者： Wang liyan Zhang Ni Sun wen Deng Yuanfa Long Yongsheng Dongfeng Technical Center

China 6 regulation《GB18352.6-2016 limits and measurement methods for emissions from light-duty vehicles》 has imported standard RDE test of Europe *** by driving style and real road condition,RDE has characteristics as its boundary uneasy to control and bad *** it is very difficult to realize accurate calibration on real test *** article aims at analyzing RDE test method ***-RDE test conditions for roller bench usage are built on the basis of the chosen RDE test conditions data of real vehicle and WLTC cycle test results on the roller *** results show that this DFTC-RDE roller bench test condition can be used in engine tasks in different powertrain adaptation on vehicle with a coverage of 90%.Instantaneous emission of DFTC-RDE roller bench test condition equals to that of WLTC test *** can represent RDE test condition instantaneous *** calibration optimization,DFTC-RDE test condition can be applied to RDE emission test.

关键词： real driving emission(RDE) indoors DFTC-RDE test condition contaminant emission engine work area

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Science China(Life Sciences) 2023年第4期66卷 729-742页

作者： Chunyan Yi Caixia Su Xiaoyu Sun Xiao Lu Chuanya Si Caixuan Liu Zhuo Yang Hong Yuan Yuying Huang Jing wen Yonghui He Yaguang Zhang liyan Ma Yao Cong Gan Zhao Zhiyang Ling Bin Wang Bing Sun State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Key Laboratory of Medical Molecular Virology(MOE/MOH) Department of Medical Microbiology and ParasitologySchool of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghai 200032China MedimScience.Co Hangzhou 311217China Shanghai Institute of Infectious Disease and Biosecurity Shanghai Medical CollegeFudan UniversityShanghai 200032China

Respiratory syncytial virus(RSV) continues to pose serious threats to pediatric populations due to the lack of a vaccine and effective antiviral drugs. RSV fusion(F) glycoprotein mediates viral-host membrane fusion and is a key target for neutralizing antibodies. We generated 23 full-human monoclonal antibodies(hm Abs) against prefusion F protein(pre-F) from a healthy adult with natural RSV infection by single B cell cloning technique. A highly potent RSV-neutralizing hm Ab, named as 25-20, is selected, which targets a new site Φ-specific epitope. Site-directed mutagenesis and structural modelling analysis demonstrated that 25-20 mainly targets a highly conserved hydrophobic region located at the a4 helix and a1 helix of pre-F, indicating a site of vulnerability for drug and vaccine design. It is worth noting that 25-20 uses an unreported inferred germline(i GL) that binds very poorly to pre-F, thus high levels of somatic mutations are needed to gain high binding affinity with pre-F. Our observation helps to understand the evolution of RSV antibody during natural infection. Furthermore, by in silico prediction and experimental verification, we optimized 25-20 with KD values as low as picomolar range. Therefore, the optimized 25-20 represents an excellent candidate for passive protection against RSV infection.

关键词： RSV human antibody potent neutralization conserved epitope somatic mutations affinity maturation

Data Analysis of Chang'E-1 Gamma-Ray Spectrometer and Global Distribution of U,K,and Th Elemental Abundances

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Acta Geologica Sinica(English Edition) 2011年第6期85卷 1299-1309页

作者： ZOU Yongliao ZHANG liyan LIU Jianzhong MU Lingli REN Xin ZHANG Guangliang CHANG Jin YAN Jun ZHANG Nan ZHANG Hongbo LU Chang LIU Jianjun ZUO Wei SU Yan wen Weibin BIAN Wei WANG Min XU Chun LI Chunlai OUYANG Ziyuan National Astronomical Observatories the Chinese Academy of Sciences Beijing 100012 China Purple Mountain Observatory the Chinese Academy of Sciences Nanjing 210008 China

Gamma-ray spectrometer （GRS） is one of the main payloads on the Chang＇E-1 （CE-1） lunar probe, mainly aimed to detect the elemental abundances and distributions on the lunar surface. At 03：58 on 28 November 2007, it performed the first observation of the lunar gamma rays. As of 24 October 2008, 2105 h of effective gamma rays spectra had been acquired by CE-1 GRS, which covers the whole surface of the moon. This paper mainly describes the data processing procedures and methods of deriving the elemental abundances by using the CE-1 GRS time series corrected spectra： first, to bin data into pixels for mapping; then, to perform a background deduction of the cumulative spectra and obtain a peak area of the elements; and finally, to use the elemental abundances inversion model to produce the elemental abundances. Based on these processing methods, the global abundance maps of U, K, and Th at a 5°×5° equal-area pixel are acquired by CE-1 GRS data. The paper gives a preliminary analysis of the uncertainties of the elemental abundances.

关键词： Chang'E-1 payloads gamma rays elemental abundances

HBV co-infection attenuate HCV neutralizing antibody response through altering circulating T follicular helper cell differentiation by HBV surface antigen

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HBV co-infection attenuate HCV neutralizing antibody respons...

Vitamin B6 regulates IL-33 homeostasis to alleviate type 2 inflammation

第十二届全国免疫学学术大会

作者： Qu Xiaowang wen Bo Zhang Jian Tang Ping Huang liyan Xie Ting Liu wenpei Translational Medicine Institute University of South China

Hepatitis C virus(HCV)and Hepatitis B virus(HBV)co-infection is very common in high risk population,we previously reported that HBV co-infection with HCV attenuate HCV glycoprotein binding activity in injection drug u... 详细信息

关键词： HBV HCV T follicular helper cell neutralizing antibody Co-infection

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Cellular & Molecular Immunology 2023年第7期20卷 794-807页

作者： Songling Zhu Shufen Zhong Kebin Cheng Li-Sha Zhang Jiu-wu Bai Zu Cao Su Wang wen Chen Shipeng Cheng liyan Ma Zhiyang Ling Yuying Huang Wangpeng Gu Xiaoyu Sun Chunyan Yi Meng Zhao Shuo Liang Jin-Fu Xu Bing Sun Yaguang Zhang Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei 230022China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of Sciences320 Yueyang RoadShanghai 200031China Department of Respiratory and Critical Care Medicine Shanghai Pulmonary HospitalInstitute of Respiratory MedicineSchool of MedicineTongji University507 Zhengmin RoadShanghai 200433China

Interleukin-33(IL-33)is a crucial nuclear cytokine that induces the type 2 immune response and maintains immune *** fine-tuned regulation of IL-33 in tissue cells is critical to control of the type 2 immune response in airway inflammation,but the mechanism is still ***,we found that healthy individuals had higher phosphate-pyridoxal(PLP,an active form of vitamin B6)concentrations in the serum than asthma *** serum PLP concentrations in asthma patients were strongly associated with worse lung function and *** a mouse model of lung inflammation,we revealed that PLP alleviated the type 2 immune response and that this inhibitory effect relied on the activity of IL-33.A mechanistic study showed that in vivo,pyridoxal(PL)needed to be converted into PLP,which inhibited the type 2 response by regulating IL-33 *** mice heterozygous for pyridoxal kinase(PDXK),the conversion of PL to PLP was limited,and IL-33 levels were increased in the lungs,aggravating type 2 ***,we found that the mouse double minute 2 homolog(MDM2)protein,an E3 ubiquitin-protein ligase,could ubiquitinate the N-terminus of IL-33 and sustain IL-33 stability in epithelial *** reduced MDM2-mediated IL-33 polyubiquitination and decreased the level of IL-33 through the proteasome *** addition,inhalation of PLP alleviated asthma-related effects in mouse *** summary,our data indicate that vitamin B6 regulates MDM2-mediated IL-33 stability to constrain the type 2 response,which might help develop a potential preventive and therapeutic agent for allergy-related diseases.

关键词： Type 2 inflammation IL-33 Vitamin B6

Ga_2O_3和P_2O_5掺入对碲钡锶铌系玻璃Raman性能的影响

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硅酸盐学报 2010年第11期38卷 2065-2070页

作者：温磊张丽艳陈伟胡丽丽中国科学院上海光学精密机械研究所高功率激光单元技术研发中心上海201800

研究了在TeO_2-BaCO_3-SrCO_3-Nb_2O_5(TBSN)玻璃体系中单独引入P_2O_5(TBSN-P)或同时引入P_2O_5和Ga_2O_3对TBSN玻璃的Raman散射和热稳定性的影响。结果表明:TBSN-P体系中,相对Raman散射截面随P_2O_5含量的增加逐渐减小,Raman增益带宽则显著增加;在TBSN-10Ga-P[(7-x)TeO_2-5BaO-5SrO-5Nb_2O_5-10Ga_2O_3-xP_2O_5(x=0,4,8,12,16,20,摩尔比,下同)]与TBSN-16P-Ga-[(69-y)TeO_2-5BaO-5SrO-5Nb_2O_5-16P_2O_5-yGa_2O_3(y=0,2,4,6,8,10)]系列中,Ga_2O_3的存在使得350～1250 cm^(-1)的整体谱形更加平坦,但Raman增益带宽和相对Raman散射截面均有所下降。计算了各玻璃样品的Raman增益系数和增益带宽,发现TBSN-4P玻璃具有最大的Raman增益系数(8.70×10^(-12)m/W),TBSN-24P玻璃具有最大Raman增益带宽(621 cm^(-1)),分别为熔石英玻璃的45倍和2.95倍。对玻璃的热稳定性研究表明,在TBSN-P体系中,P_2O_5的引入可以使玻璃的热稳定性能显著提高,而在Ga_2O_3与P_2O_5同时被引入时,Ga_2O_3含量的增加会导致玻璃的热稳定性变差。

关键词： Raman放大 Raman增益碲酸盐玻璃

基于区间熵物元模型的烟草工业立体仓库物流效率评价研究

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中国烟草学报 2018年第3期24卷 113-119页

作者：车文刀荣贵杨立言红塔烟草(集团)责任有限公司物流中心

【目的】为有效评估烟草工业企业立体仓库物流效率,对A烟草工业企业立体仓库的物流作业进行分析。【方法】建立烟草工业企业立体仓库物流效率指标评价体系,对各项评价指标进行定义,引用区间熵值法用于权重的计算,基于物元理论建立综合... 详细信息

【目的】为有效评估烟草工业企业立体仓库物流效率,对A烟草工业企业立体仓库的物流作业进行分析。【方法】建立烟草工业企业立体仓库物流效率指标评价体系,对各项评价指标进行定义,引用区间熵值法用于权重的计算,基于物元理论建立综合评价模型。【结果】最后,采集A烟草工业企业立体仓库运营期间的实际数据,对烟草工业立体仓库物流效率进行综合评价,其综合评价等级为"良"。【结论】结果验证了该综合评价方法的可行性,对改善仓库物流效率具有一定指导意义。

关键词：烟草工业立体仓库物元模型熵值法物流效率评价指标

集体声誉、产权性质与债务融资——基于中国高校系上市公司的准自然实验

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中国软科学 2022年第4期 183-192页

作者：章刚勇王立彦文芳广东金融学院广东广州510520 北京大学光华管理学院北京100871

我国A股市场较为独特的高校系上市公司为研究集体声誉如何作用于个体行为提供了一项准自然实验,从债务融资规模和期限结构等视角研究了集体声誉、产权性质与企业外部债务融资之间的关系。研究发现,集体声誉对上市公司的融资规模具有较... 详细信息

我国A股市场较为独特的高校系上市公司为研究集体声誉如何作用于个体行为提供了一项准自然实验,从债务融资规模和期限结构等视角研究了集体声誉、产权性质与企业外部债务融资之间的关系。研究发现,集体声誉对上市公司的融资规模具有较显著的正向作用,但当个体行为能较好地被市场观测时,集体声誉作用较弱,个体声誉作用较强,表现为与其他非高校系上市公司相比,高校系上市公司的债务期限较短。此外,随着以商业银行为主体的借贷市场市场化程度的提高,样本期间,银行信贷政策并未偏袒于经行业和资本规模匹配的国有上市公司。因此,高度重视高校声誉对高校系上市公司的影响,完善高校系上市公司治理结构至关重要,深入推进金融和产权市场化改革。

关键词：集体声誉债务融资高校系上市公司

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天然气水合物横波速度等效介质模型预测方法

石油地球物理勘探 2020年第1期55卷 117-125,8页

作者：孟大江文鹏飞张如伟赵斌李延自然资源部海底矿产资源重点实验室广东广州510075 广州海洋地质调查局广东广州510075

由于天然气水合物具有多种充填模式,水合物既可作为固体骨架又可作为孔隙充填物赋存于地层中,因此天然气水合物岩石物理模型与常规油气的岩石物理模型具有较大的差异,常规横波预测方法不再适用。为此,开展了利用等效介质模型进行天然气... 详细信息

由于天然气水合物具有多种充填模式,水合物既可作为固体骨架又可作为孔隙充填物赋存于地层中,因此天然气水合物岩石物理模型与常规油气的岩石物理模型具有较大的差异,常规横波预测方法不再适用。为此,开展了利用等效介质模型进行天然气水合物横波预测的方法研究。首先分析矿物组分、孔隙度和水合物饱和度对纵横波速度的影响;然后利用纵波时差、密度、泥质含量、孔隙度、饱和度等常规测井资料根据等效介质模型构建约束优化方程,其中以纵波速度、密度等常规测井资料为约束,孔隙度和饱和度作为优化变量以寻找最优解;采用收敛快、计算结果可靠的信赖域算法求解该约束最优化问题。最后利用神狐海域天然气水合物的钻井资料对方法进行验证,计算的横波速度和饱和度与实际资料吻合度较高。

关键词：等效介质模型天然气水合物岩石物理横波速度预测约束最优化