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Frontiers of Chemical Science and Engineering 2016年第3期10卷 396-404页

作者： weixia wang Shuai Zhou Zhong Xin Yaoqi Shi Shicheng Zhao Shanghai Key Laboratory of Multiphase Materials Chemical Engineering East China University of Science and Technology Shanghai 200237 China State Key Laboratory of Chemical Engineering East China University of Science and Technology Shanghai 200237 China Shanghai Key Laboratory of Catalysis Technology for Polyolefin Shanghai Research Institute of Chemical Industry Shanghai 200062 China

Foamable high melt strength polypropylene （HMSPP） was prepared by grafting styrene （St） onto polypropylene （PP） and simultaneously introducing poly- dimethylsiloxane （PDMS） through a one-step melt extru- sion process. The effect of PDMS viscosity on the foaming behavior of HMSPP was systematically investigated using supercritical CO2 as the foaming agent. The results show that the addition of PDMS has little effect on the grafting reaction of St and HMSPP exhibits enhanced elastic response and obvious strain hardening effect. Though the CO2 solubility of HMSPP with PDMS （PDMS-HMSPP） is lower than that of HMSPP without PDMS, especially for PDMS with low viscosity, the PDMS-HMSPP foams exhibit narrow cell size distribution and high cell density. The fracture morphology of PDMS-HMSPP shows that PDMS with low viscosity disperses more easily and uniformly in HMSPP matrix, leading to form small domains during the extrusion process. These small domains act as bubble nucleation sites and thus may be responsible for the improved foaming performance of HMSPP.

关键词： high melt strength polypropylene (HMSPP),polydimethylsiloxane (PDMS) supercritical CO2 foaming behavior

Cloning and Functional Analysis of Calcineurin Subunits A and B in Development and Fecundity of Nilaparvata lugens(Stål)

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Rice science 2022年第2期29卷 143-154页

作者： wang weixia ZHU Tingheng WAN Pinjun WEI Qi HE Jiachun LAI Fengxiang FU Qiang State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China College of Biotechnology and Bioengineering Zhejiang University of TechnologyHangzhou 310014China

Serine/threonine phosphatase calcineurin(CN)is a unique but confounding calcium/calmodulin-mediated enzyme,which is composed of a catalytic subunit A(CNA)and a regulatory subunit B(CNB).We cloned six transcripts for CNA named from NlCNA-X1 to NlCNA-X6,one CNB named NlCNB1 and one CNB homologous gene NlCNBH1 from Nilaparvata *** of them are constitutively transcripted in various tissues and developmental *** primary structure of the six isoforms showed obvious differences in the length and composition of the amino acid sequence between the two binding domains of Ca^(2+)/calmodulin(CaM)and ***^(2+)-binding EF-hand motifs were found in NlCNB1 and *** specific gene silencing of NlCNA,NlCNB1 and NlCNBH1 respectively by RNAi resulted in drastical reduction in survival rate,female weight,eclosion rate and fecundity of *** results showed that NlCNA,NlCNB1 and NlCNBH1 were required for *** growth and *** negative effects of NlCNB1 silence on nymph mortality(97%),molting malformation(90%)and female sterile(50%)were more serious than those of NlCNA or ***-PCR and enzyme-linked immunosorbent assay(ELISA)analyses indicated that the nymphs with silenced NlCNA,NlCNB1 or NlCNBH1 showed impaired hormone and energy *** nymphs,the contents of 20-hydroxyecdysone(20E)after NlCNB1 RNAi and phenoloxidase after NlCNA RNAi were particularly *** results suggested that NlCNA is involved in immunity of *** by regulation of phenoloxidase,while NlCNB1 may control the growth and development of *** by 20E signaling pathway in addition to interact with *** of 70 ng/μL dsNlCNB1 resulted in 77.0%down regulation of NlCNB1,and the nymph mortality was up to 57.9%at 10 d after ***,NlCNB1 could be a potential candidate target used for strategy design in control of *** results revealed the importance of CN in the regulation of the growth and development of ***,which provided

关键词： Nilaparvata lugens calcineurin calcineurin catalytic subunit calcineurin regulatory subunit 20-hydroxyecdysone phenoloxidase

Versatile functions of RNA m6A machinery on chromatin

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Journal of Molecular Cell Biology 2022年第3期14卷 3-11页

作者： Tanjing Song Suli Lv Neng Li Xuefeng Zhao Xianyun Ma Yingying Yan weixia wang Lidong Sun Department of Biochemistry and Molecular Biology School of Basic Medicineongji Medical College Huazhong University of Science and TechnologyWuhan 430030China

m6A,a conserved and abundant modification on RNA,regulates RNA processing and *** m6A machinery,including writers,erasers,and readers of m6A,is indispensable for m6A installation and ***,recent studies have revealed that m6A machinery can be recruited to chromatin by pleiotropic factors,including nascent RNA,transcription factors,regulatory RNA,histone modifications,and epigenetic ***,recruitment of m6A machinery can directly regulate chromatin biology,such as transcription,DNA damage repair,and DNA recombination beyond installation of m6A on nascent ***,we discuss recent evidence showing that m6A machinery is targeted to chromatin and the direct biological consequences along with the underlying mechanisms.

关键词： m6A chromatin RNA methylation histone modifications epigenetics

The difference between multi-drug resistant cell line A549/Gem and its parental cell A549

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The Chinese-German Journal of Clinical Oncology 2009年第4期8卷 190-194页

作者： weixia wang Xiaoqing Liu Chuanhao Tang Department of Lung Cancer Cancer Center of PLA The Affiliated 307 Hospital Academy of Military Medical Sciences Beijing 100071 China

Objective:To discuss the difference between multi-drug resistant cell line A549/Gem and its parental cell A549 on the basis of establishment of human gemcitabine-resistant cell line A549/Gem so as to elaborate the possible mechanisms of gemcitabine ***:Human gemcitabine-resistant non-small cell lung cancer cell line A549/Gem was estab-lished by the method of repeated clinical serous peak concentration plus gradually increasing concentration of gemcitabine from its parental cell human lung adenocarcinoma cell line A549 which was sensitive to *** the course of inducement,we had monitored their morphology,checked their resistance indexes and resistant pedigree by MTT method,gathered their growth curves and calculated their doubling time,examined their DNA contents and cell cycles by FCM;at the same time,we had measured their expressions of P53,EGFR,Cerb-B-2,PTEN,PCNA,c-myc,VEGF,MDR-1,Bcl-2,nm23,MMP-9,TIMP-1,and CD44v6 proteins via immunocytochemistry staining,RRM1 and ERCC1 mRNA by real-time fluorescent ***:The resistance index of A549/Gem' cells(the deputy of cells in the process of inducement) to gemcitabine was 163.228,and the cell line also exhibited cross-resistance to vinorelbine,taxotere,fluorouraci,etoposide and cisplatin,but kept sensitivity to paclitaxol and *** doubling time of A549/Gem' was shorter and figures in G0-G1 phases were increased than A549 *** with A549 cells,A549/Gem' cells achieved EGFR and c-myc proteins expressions,nm23 protein expression enhanced,P53,Cerb-B-2 and Bcl-2 proteins expressions reduced,PTEN,PCNA and MDR-1 proteins expressions vanished,but those of MMP-9,VEGF,CD44v6 and TIMP-1 proteins changed ***,expressions of RRM1 and ERCC1 mRNA were augmented *** resistance index of A549/Gem cells to gemcitabine was 129.783,and the cell line also held cross-resistance to vinorelbine,taxotere,etoposide,cisplatin and sensitivity to *** t

关键词： gemcitabine multi-drug resistance non-small cell lung cancer (NSCLC) gene

Eco-budget-A Model of Urban Environmental Management

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Ecological Economy 2005年第4期1卷 13-17页

作者： Hao weixia Teng Li wang Ziyan Research Center of Development in 21^st Century Dalian （Jniuersit） of Technology Dalian 116023 PR.China

In this paper, a new model of urban environmental resource management is introduced. The article analyzes the gap between urban environmental management and the management of economy and human resources. The significa... 详细信息

关键词： environmental management eco-budget

Research on Key Technology of Efficient Production of Bovine Embryos

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Agricultural Biotechnology 2020年第4期9卷 144-145,148页

作者： Guizhi TONG Hongguang wang Yongsheng HAN Xintao LI weixia wang Bin SONG Branch of Animal Husbandry and Veterinary Medicine Heilongjiang Academy of Agricultural SciencesQiqihar 161005China Technical Identification Station for Agricultural Products and Veterinary Feed in Heilongjiang Province Harbin 150000China Branch of Animal Science Jilin Academy of Agricultural SciencesChangchun 136100China

[Objectives]This study was conducted to explore the effects of three different splitting fluids on the bisection effects of bovine morulae and blastocysts.[Methods]With the help of a micromanipulator,the morulae and blastocysts that were routinely produced in the body from the 6 th to the 8 th d were divided into half-embryos in vitro to observe their development,and the half-embryos with a restored morphology and a blastocyst trophoblast were selected.[Results]The success rates of bisection of morulae in PBS+0.2 mol/L sucrose and PBS+5%PVP were significantly higher than that in PBS(P0.05).When blastocysts were bisected in PBS+0.2 mol/L sucrose and PBS+5% PVP,the success rates of bisection were significantly higher than that of PBS(P0.05).The development rate of half-embryos bisected from blastocysts was significantly higher than that of morulae.[Conclusions]This study improves the efficiency of bovine embryo bisection,which is beneficial to the application of embryo bisection technology in cattle breeding.

关键词： Cattle Embryo bisection Blastocyst development rate

Effects of Bovine Embryos at Different Developmental Stages on Bisection Effect

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Agricultural Biotechnology 2021年第5期10卷 60-62页

作者： Guizhi TONG Yongsheng HAN Hongbao wang Xintao LI weixia wang Xueying SONG Branch of Animal Husbandry and Veterinary Heilongjiang Academy of Agricultural SciencesQiqihar 161005China Jilin Academy of Agricultural Sciences Changchun 136100China

[Objectives]This study was conducted to explore the effects of embryos at different developmental stages on the bisection effect of embryos,improve the efficiency of bovine embryo bisection,and facilitate the application of embryo bisection technology in cattle breeding.[Methods]The effects of two different bisection solutions on the bisection of morulae and blastocysts in vitro were *** morulae and blastocysts produced in vitro from cattle that developed to the 6th to 8th d were bisected by hands,and demi-embryos were cultured in *** development was observed.[Results]Morulae were bisected in PBS solution and PBS+0.2 mol/L sucrose,and the success rates of bisection were 50%and 95.2%,*** success rate of bisecting morulae in PBS+0.2 mol/L sucrose was significantly higher than that in PBS(P0.05).The success rates of blastocyst bisection in PBS solution and PBS+0.2 mol/L sucrose were 51.6%and 95.1%,*** success rate of blastocyst bisection in PBS+0.2 mol/L sucrose was significantly higher than that in PBS(P0.05).[Conclusions]There were no significant differences between the success rates of bisecting bovine morulae and blastocysts in PBS+0.2 mol/L sucrose,which were both significantly better than those in pure PBS bisection solution,proving that PBS+0.2 mol/L sucrose bisection solution is suitable for bovine embryo bisection.

关键词： Cattle Embryo division Success rate Development rate Morula Blastocyst