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Deciphering and engineering high-light tolerant cyanobacteria for efficient photosynthetic cell factories

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Chinese Journal of Chemical Engineering 2021年第2期34卷 82-91页

作者： Yaru Xie Lei Chen Tao Sun weiwen zhang Laboratory of Synthetic Microbiology School of Chemical Engineering&TechnologyTianjin UniversityTianjin 300072China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering Ministry of Education of ChinaTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072China Center for Biosafety Research and Strategy Tianjin UniversityTianjin 300072China Law School Tianjin UniversityTianjin 300072China

Development and utilization of“liquid sunshine”could be one of key solutions to deal with the issues of fossil fuel depletion and increasing carbon *** are the only prokaryotes capable of performing oxygenic photosynthesis,and their activity accounts for~25%of the total carbon fixation on *** importantly,besides their traditional roles as primary producers,cyanobacteria could be modified as“photosynthetic cell factories”to produce renewable fuels and chemicals directly from CO_(2) driven by solar energy,with the aid of cutting-edging synthetic biology *** their large-scale biotechnological application in the future,many challenges still need to be properly addressed,among which is cyanobacterial cell factories inevitably suffer from high light(HL)stress during large-scale outdoor cultivation,resulting in photodamage and even cell death,limiting their *** this review,we critically summarized recent progress on deciphering molecular mechanisms to HL and developing HL-tolerant chassis in cyanobacteria,aiming at facilitating construction of HLresistant chassis and promote the future application of the large-scale outdoor cultivation of cyanobacterial cell ***,the future directions on cyanobacterial chassis engineering were discussed.

关键词： High-light Stress Chassis Cell factories Cyanobacteria

Effect of microstructure on the mechanical and corrosion behaviors of a hot-extruded nickel aluminum bronze

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Acta Metallurgica Sinica(English Letters) 2010年第2期23卷 113-120页

作者： Datong zhang Ruiping CHEN weiwen zhang Zongqiang LUO Yuanyuan LI School of Mechanical and Automotive Engineering South China University of Technology Guangzhou 510640 China

In this paper, the influence of microstructure on the corrosion behavior of a hotextruded nickel aluminum bronze was studied. Three kinds of samples subjected to the hot-extrusion, anneMing and quenching conditions were prepared and immersion tests in 3.5% NaCl solution were carried out. Microstructures and corrosion surface morphologies of the samples were observed by SEM. It was found that the retained β＇ martensite and （α＋kiii） lamella eutectoid in the as hot-extruded material were eliminated after annealing, and corrosion resistance of the alloy was improved. As to the as-quenched material, its corrosion rate was higher than that of the as hotextruded material since the volume fraction of β＇ phase which was anodic to α phase increased after quenching. As regards the mechanical properties, the as-quenched sample possesses the highest hardness and tensile strength among the three kinds of samples, while the as-annealed sample possesses the highest elongation. That is to say, heat treatment plays different roles in the mechanical properties and corrosion resistance of the experimental alloy.

关键词： Nickel aluminum bronze Heat treatment Microstructure Mechanical properties Corrosion behavior

The Philosophy of ＂Naturalness＂ in the Laoziand Its Value For Contemporary Society

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Frontiers of Philosophy in China 2017年第3期12卷 340-357页

作者： zhang weiwen School of Philosophy Beijing Normal University Beijing 100875 China

This article aims to show that the concept of ＂naturalness＂ in the Laozi is able to provide cultural guidance concerning values for contemporary social development. Specifically, the Laozi＇s concept of ＂naturalness＂-- manifested in the text＇s exhortation to ＂honor the dao and exalt the de＂ and its statement that ＂the dao models itself on naturalness＂--has profound ontological, political and social implications concerning ＂naturalness＂ that are strongly expressed through a variety of propositions including ＂achieving all through non-action＂ and ＂downsizing the state and simplifying the people.＂ With respect to the question about individuals living a life of appropriateness and establishing their destiny, the Laozi emphasizes such cultivation methods as ＂sticking to simplicity and authenticity＂ and ＂watching in quietude and observing in depth,＂ which are also infused with the conception of ＂naturalness,＂ which stresses the notion that understanding the harmony between man and nature can provide useful lessons for the development of contemporary human society.

关键词： Laozi nature philosophy value

Towards understanding the mechanism of n-hexane tolerance in Synechocystis *** 6803

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Chinese Journal of Chemical Engineering 2023年第7期59卷 128-134页

作者： Tao Sun Shubin Li Guangsheng Pei Lei Chen weiwen zhang Laboratory of Synthetic Microbiology School of Chemical Engineering&TechnologyTianjin UniversityTianjin 300072China Frontier Science Center for Synthetic Biology&Key Laboratory of Systems Bioengineering Ministry of Education of ChinaTianjin 300072China Center for Biosafety Research and Strategy Tianjin UniversityTianjin 300072China

Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as"autotrophic cell factories"for biosynthesis of various biofuels directly from CO_(2).However,the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial *** this study,RNAseq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis *** *** enrichment analysis of the transcriptomic data showed that signal transduction systems were induced *** further identify regulatory genes related to n-hexane tolerance,a library of transcriptional regulators(TRs)deletion mutants was then screened for their roles in nhexane *** results showed that a knockout mutant of slr0724 that encodes an Hta R suppressor protein was more tolerant to n-hexane than the wild type,indicating the involvement of slr0724 in nhexane *** study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis *** 6803,which could contribute to the further engineering of nhexane tolerance in cyanobacteria.

关键词： Transcriptome n-Hexane tolerance Metabolomics Synechocystis

An Al–Al interpenetrating-phase composite by 3D printing and hot extrusion

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International Journal of Minerals,Metallurgy and Materials 2023年第4期30卷 678-688页

作者： Yulin Lin Di Wang Chao Yang weiwen zhang Zhi Wang School of Mechanical and Automotive Engineering South China University of TechnologyGuangzhou 510640China Guangdong Key Laboratory for Processing and Forming of Advanced Metallic Materials South China University of TechnologyGuangzhou 510640China

We report a process route to fabricate an Al–Al interpenetrating-phase composite by combining the Al–Mg–Mn–Sc–Zr lattice structure and Al_(84)Ni_(7)Gd_(6)Co_(3)nanostructured structure. The lattice structure was produced by the selective laser melting and subsequently filled with the Al_(84)Ni_(7)Gd_(6)Co_(3)amorphous powder, and finally the mixture was used for hot extrusion to produce bulk samples. The results show that the composites achieve a high densification and good interface bonding due to the element diffusion and plastic deformation during hot *** bulk samples show a heterogeneous structure with a combination of honeycomb lattice structure with an average grain size of less than1 μm and nanostructured area with a high volume fraction of nanometric intermetallics and nanograin α-Al. The heterogeneous structure leads to a bimodal mechanical zone with hard area and soft area giving rise to high strength and acceptable plasticity, where the compressive yield strength and the compressive plasticity can reach ~745 MPa and ~30%, respectively. The high strength can be explained by the rule of mixture,the grain boundary strengthening, and the back stress, while the acceptable plasticity is mainly owing to the confinement effect of the nanostructured area retarding the brittle fracture behavior.

关键词： Al-based composites heterogeneous structure additive manufacturing mechanical properties

Synthetic biology:Recent progress,biosafety and biosecurity concerns,and possible solutions

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Journal of Biosafety and Biosecurity 2019年第1期1卷 22-30页

作者： Fangzhong Wang weiwen zhang Center for Biosafety Research and Strategy Tianjin UniversityTianjinPR China Laboratory of Synthetic Microbiology School of Chemical Engineering and TechnologyTianjin UniversityTianjinPR China Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjinPR China SynBio Research Platform Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)PR China

Synthetic biology is a new interdisciplinary research area that uses engineering principles as guidelines for biological *** research goals to modify existing biological systems or to create new ones,the recent applications of synthetic biology have expanded approaches and tools for conventional biological *** this article,we first briefly review the development and progress of synthetic biology over the past *** the contributions of synthetic biology to basic life science research,human health,environmental protection,and even economic growth have been widely observed,potential biosafety,biosecurity,and ethical risks related to synthetic biology have also emerged in recent years as technology becomes less expensive,more mature,and more *** provide a brief assessment of the risks associated with the possible misuse or abuse of this technology in various areas and discuss concerns from three points of view:biosafety,biosecurity risks,and ***,to address challenges arising from the rapid progress of synthetic biology,technical,ethical,and regulatory measures were developed or discussed in recent years,including laboratory level precautionary measures for biosafety and biosecurity related to synthetic biology(such as genetic safeguards and firewalls),ethical codes of conduct for biological scientists,and regulations or oversight rules from personal,national,and international perspectives.A brief summary of these efforts is provided.

关键词： Synthetic biology Biosafety Biosecurity Ethics Genetic firewall Genetic safeguard Code of conduct Governance regulation

Identification of genes involved in cadmium-ion tolerance in evolutionary Synechocystis *** 6803 tolerant to both cadmium and high light

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Chinese Journal of Chemical Engineering 2024年第10期74卷 74-82页

作者： Jie Xiong Zhengxin Dong Yaru Xie weiwen zhang Tao Sun Lei Chen Laboratory of Synthetic Microbiology School of Chemical Engineering&TechnologyTianjin UniversityTianjin 300072China Frontier Science Center for Synthetic Biology&Key Laboratory of Systems Bioengineering Ministry of Education of ChinaTianjin 300072China Center for Biosafety Research and Strategy Tianjin UniversityTianjin 300072China

Photosynthetic cyanobacteria have shown great potential as“autotrophic cell factories”for the synthesis of fuels and ***,poor tolerance to various environmental stressors such as high light and heavy metals is an important factor limiting their economic *** numerous studies have focused on the tolerance mechanism of cyanobacteria to individual stressors,their response to simultaneous stresses remains to be *** investigate the mechanism of cross tolerance to heavymetal Cd^(2+) and high light,the model cyanobacterium Synechocystis *** 6803 tolerant to both Cd^(2+) and high light was obtained via about 800 days’cross-adaptive laboratory *** evolutionary strains capable of tolerating both 5.5 μmol·L^(-1) Cd^(2+) and 600 μmol·m^(-2)·s^(-1) high light were successfully obtained,achieving about 83%enhancement of Cd^(2+) tolerance compared with the parent *** different response of parent and evolutionary strains to Cd^(2+) was elucidated via ***,a total of 15 genes that were mutated during evolution were identified by whole-genome ***,by single-gene knockout and complementation analysis,four genes including ssl2615,sll1732,ssr1480,and sll1659 involved in the improvement of Cd^(2+) tolerance under high-light condition were successfully *** work explored the tolerance mechanism of Synechocystis *** 6803 to cadmium under high-light condition and provided valuable reference for deciphering multitolerance mechanism of cyanobacteria in the future.

关键词： Synechocystis Adaptive laboratory evolution Cross tolerance Cadmium-ion tolerance

Control of equiaxed grains in a complicated Cu-Ni based alloy prepared by centrifugal casting

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China Foundry 2011年第1期8卷 141-144页

作者： Luo Zongqiang zhang weiwen Xin Baoliang Li Yuanyuan School of Mechanical and Automotive Engineering South China University of Technology Guangzhou 510640 China

A complicated Cu-Ni based alloy was developed to fabricate wear-resisting bush for high temperature *** concern focuses on the control of equiaxed grains in the developed alloy ingot prepared by centrifugal *** results show that the equiaxed grains are determined by the pouring temperature of the melt,the cooling rate and the rotation speed of the *** the decrease in pouring temperature,the fraction of the equiaxed grains in the transverse section of the ingot increases and the average length of columnar grain *** the pouring temperature is confined below 1,250℃,complete equiaxed grains can be *** on the optimal centrifugal casting processing,the tensile strength of the developed alloy ingot with complete equiaxed grains reaches to 810 MPa and 435 MPa at room temperature and 500℃,respectively,which is 14% and 110% higher than that of common commercial QAl10-4-4 *** wear rate of the developed alloy is 7.0 × 10-8 and 3.8 × 10-7 mm3?N-1?mm-1 at room temperature and 500℃,respectively,which is 5 times and 39 times lower than that of QAl10-4-4 alloy.

关键词： copper-nickel based alloy centrifugal casting macrostructures

Human genome editing after the "CRISPR babies":The double-pacing problem and collaborative governance

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Journal of Biosafety and Biosecurity 2023年第1期5卷 8-13页

作者： Leifan Wang Lijun Shang weiwen zhang Tianjin University School of Law Tianjin 300072China Center for Biosafety Research and Strategy Tianjin UniversityTianjin 300072China School of Human Sciences London Metropolitan UniversityLondon N78DBUK Biological Security Research Centre Metropolitan UniversityLondon N78DBUK School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

How to ensure the safe,effective,and ethical use of emerging biotechnologies,such as clustered regularly interspaced short palindromic repeats(CRISPR)-based genome editing,is a global *** occurrence of the"CRISPR babies"in 2018 publicly brought this issue into sharp focus,and led to comprehensive regulatory reforms in China and various countries around the *** current article analyzes this event-driven regulatory reform in China by elaborating the most salient provisions designed to prevent risk and protect individual rights,public health,and social morality relating to human genome editing in four important sectors of law:biosecurity law,civil code,criminal law and patent *** highlights that,although regulation is being undertaken,the gaps between the law and advancing technology remain discernible,at both a national and transnational level(i.e.,the "double-pacing problem").Further attention and collaboration will be required to address the ongoing challenges associated with the use of human genome editing.

关键词： Human genome editing CRISPR babies Double-pacing problem Collaborative governance China