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Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors

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Journal of Environmental Sciences 2008年第6期20卷 732-738页

作者： YAN Qingyun YU Yuhe feng weisong YU Zhigang CHEN Hongtao Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Ocean University of China Qingdao 266003 China

To explore the relationships between community composition and the environment in a reservoir ecosystem, plankton communities from the Three Gorges Reservoir Region were studied by PCR-denaturing gradient gel electrophoresis fingerprinting. Bacterial and eukaryotic operational taxonomic units （OTUs）, generated by DGGE analysis of the PCR-amplified 16S and 18S rRNA genes, were used as surrogates for the dominant ＂biodiversity units＂. OTU composition among the sites was heterogeneous; 46.7% of the total bacterial OTUs （45） and 64.1% of the eukaryotic OTUs （39） were identified in less than half of the sampling sites. Unweighted pair group method with arithmetic averages （UPGMA） clustering of the OTUs suggested that the plankton communities in the Xiangxi Rive sites were not always significantly different from those from the Yangtze River sites, despite clear differences in their environmental characterizations. Canonical correspondence analysis （CCA） was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of NO3^--N, dissolved oxygen （DO）, and SiO3^2--Si, whereas, the eukaryotic community was mainly correlated with the concentrations of DO, PO4^3--P, and SiO3^2--Si.

关键词： plankton community environmental factors denaturing gradient gel electrophoresis (DGGE) spatial pattern Three Gorges Reservoir Region

Impacts of algal blooms removal by chitosan-modified soils on zooplankton community in Taihu Lake,China

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Journal of Environmental Sciences 2010年第10期22卷 1500-1507页

作者： Jiajia Ni Yuhe Yu weisong feng Qingyun Yan Gang Pan Bo Yang Xiang Zhang Xuemei Li Key Laboratory of Biodiversity and Conservation of Aquatic Organisms Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China. Graduate School of the Chinese Academy of Sciences Beijing 100039 China State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China

It is important to assess the effect on zooplankton when perform the environmental protection or restoration technology, especially removing algal blooms, because algae were the major primary producer in algal lakes. The influence on zooplankton community after half a year of algal blooms removed by chitosan-modified soils in Taihu Lake was assessed and the rationality of carrying out the process semiannually was evaluated in the present study. Morphological composition and genetic diversity of zooplankton community were investigated by microscope checkup and polymerase chain reaction-denatured gradient gel electrophoresis （PCR-DGGE）. A total of 44 zooplankton taxa （23 protozoa, 17 rotifers, 3 copepoda and 1 cladocera） were detected by microscope checkup, and a total of 91 bands （28 bands amplified by primers F1427-GC and R1616, 63 bands amplified by primers Fung-GC and NS1） were detected by PCR-DGGE. The results of cluster analysis or detrended correspondence analysis indicated that there was no considerable difference in morphological composition of zooplankton and DGGE profiles between experimental and control sites, and DGGE profiles could represent the biologic diversity. The study showed that zooplankton community could recover original condition after half year of algal blooms removed by chitosan-modified soils and it was acceptable to apply this process semiannually. In addition, the results revealed that PCR-DCJGE could be applied to investigate the impacts of the environmental protection or restoration engineering on zooplankton community diversity.

关键词： algal blooms Microcystis aeruginosa zooplankton community chitosan-modified soils Taihu Lake

Restoration processes of pollution zones in Hanjiang River

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Journal of Environmental Sciences 1996年第2期8卷 178-184页

作者： Shen Yunfen Tan Yuyun feng weisong Gu Manru (Institute of Hydrobiology, Chinese Academy of Sciences, Wuchang 430072, China) He Chengying (Environmental Protection Station of Xiantao City ,China) Li Aiguo (Environmental Protection Station of Xiangfan Ci Institute of Hydrobiology Chinese Academy of Sciences Wuchang 430072 China Environmental Protection Station Xiantao City China Environmental Protection Station Xiangfan City China

Two pollution zones in middle and lower reaches of Hanjiang River were selected for studying restoration processes. In each zones 6 stations were set up in upper stream of sewage outfall, 50m, 100 or 150m, 250 or 525m and 1250 or 3500m apart from the outfall. Chemical monitoring and microbial community biomonitoring were carried out simultaneously. Either the chemical monitoring or the biological monitoring proved the self-purification process of water body along with the increased distance from the sewage outfall. 4 biological parameters (species number of protozoa, percentage of phytomastigophora, diversity index and heterotrophy index) and parameter Seq of the colonization process all have statistically significant correlations with chemical comprehensive pollution indexes Pa and Pb.

关键词： restoration process microbial community biomonitoring river pollution zone.

Genetic fingerprinting of plankton community provides new insights into aquatic ecology

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Progress in Natural Science:Materials International 2006年第9期16卷 893-898页

作者： YAN Qingyun, YU Yuhe and feng weisong ( Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China Graduate School of the Chinese Academy of Sci-ences, Beijing 100039, China) Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Over the past two decades, molecular techniques have been widely used in ecological study and molecular ecology has been one of the most important branches of ecology. Meanwhile, genetic fingerprinting analyses have significantly enhanced our knowledge of the diversity and evolutionary relations of the planktonic organisms. Compared with conventional approaches in ecological study (e.g. morphological classification), genetic fingerprinting techniques are simpler and much more effective. This review provides an overview of the principles, advantages and limitations of the commonly used DNA fingerprinting techniques in plankton research. The aim of this overview is to assess where we have been, where we are now and what the future holds for solving aquatic ecological problems with molecular-level information.

关键词： genetic fingerprinting plankton community aquatic ecology genetic diversity.

ECOLOGICAL STUDY ON PROTOZOA IN THE SEDIMENT OF THE THREE-GORGES AREA OF THE CHANGJIANG RIVER

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Chinese Journal of Oceanology and Limnology 1988年第3期6卷 272-280页

作者：顾曼如冯伟松沈韫芬 Institute of Hydrobiology Academia Sinica Wuhan

This paper reports on the study on the protozoan community structure of the immersed sediment in the Three-Gorges Area of the Changjiang River. Protozoa observed included 7 phytoflagellates, 12 zooflagellates, 49 amoebae and 16 ciliates. Sarcodina dominated in sediment, ciliates in water. Analysis of species and horizontal density distribution of protozoan in sediment in 28 sampling points reflected the pollution degree of water quality at every site. It was shown that heavy metal contamination in sediment has some effects on the distribution of protozoan species in the Changjiang River.

关键词： contamination immersed dominated terrestrial interested sedimentation grateful appeared river dilute

污水处理厂中浮游生物群落DNA指纹及其与水质指标的关系

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环境科学学报 2008年第8期28卷 1526-1533页

作者：张翔余育和冯伟松周伟颜庆云中国科学院水生生物研究所武汉市龙王嘴污水处理厂武汉430079

以武汉龙王嘴污水处理系统为研究对象,揭示了污水处理各阶段中浮游生物群落的DNA指纹拓扑结构,进而探索了其与浮游生物群落结构和环境理化因子的关系.首先建立了污水处理系统中浮游生物群落总DNA提取方法,然后用原核与真核特异性引物对... 详细信息

以武汉龙王嘴污水处理系统为研究对象,揭示了污水处理各阶段中浮游生物群落的DNA指纹拓扑结构,进而探索了其与浮游生物群落结构和环境理化因子的关系.首先建立了污水处理系统中浮游生物群落总DNA提取方法,然后用原核与真核特异性引物对流程(A2/O氧化沟工艺)中不同阶段的浮游生物群落总DNA进行PCR扩增,用变性梯度凝胶电泳(DGGE)检测,并对平行水样分别进行常规理化因子和浮游生物物种的检测与鉴定.结果显示,各采样点理化因子、物种组成与浮游生物DNA指纹的统计分析结果十分吻合,厌氧、缺氧和好氧阶段间差异较小,进水、出水分别与其它采样点间差异较大.研究结果表明,污水中浮游生物群落具有丰富的多样性,其DNA指纹在空间距离较短的污水处理过程中发生了改变,而且其改变的趋势与生物组成和理化指标的趋势相符,说明浮游生物群落DNA指纹与水质指标密切相关.

关键词：浮游生物 DNA指纹理化指标物种组成 PCR-DGGE 活性污泥

Microsatellite DNA Markers for Euglena mutabilis SchmitzFACHB-851

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Microsatellite DNA Markers for Euglena mutabilis SchmitzFACH...

湖北省西凉湖浮游动物群落周年动态变化及其与环境因子的关系

中国动物学会原生动物学分会第十二次学术讨论会

作者： Zhang wenjing, Yu yuhe, Shen yunfen, Miao Wei and feng weisong (Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China)

＜正＞ Microsatellite loci of one species may be applied in other closely related species, so that tedious procedures to isolate microsatellite markers in a novel species may not be necessary. Isolation of microsatellite markers was not previously reported in Euglena mutabilis. Here we report the cross-species amplification of microsateUite primers designed from the Euglena gracilis FACHB-848 in ***.

关键词： Microsatellite loci Euglena mutablilis sequences

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湖泊科学 2011年第4期23卷 619-625页

作者：吴利冯伟松张堂林徐昊余育和合肥师范学院生命科学系合肥230061 中国科学院水生生物研究所武汉430072 皖南医学院药学系芜湖241000

2007年11月至2008年10月对西凉湖浮游动物进行了4次调查,共检出浮游动物224种,其中原生动物112种,轮虫90种,枝角类15种,桡足类7种.秋季浮游动物物种数最多,为111种,冬季最少,为75种,春季和夏季分别为107种和88种.冬季浮游动物丰度最高,... 详细信息

2007年11月至2008年10月对西凉湖浮游动物进行了4次调查,共检出浮游动物224种,其中原生动物112种,轮虫90种,枝角类15种,桡足类7种.秋季浮游动物物种数最多,为111种,冬季最少,为75种,春季和夏季分别为107种和88种.冬季浮游动物丰度最高,为126731ind./L,春季最低,为71356ind./L,夏季和秋季分别为109743ind./L和107650ind./L,原生动物和轮虫的丰度是西凉湖浮游动物丰度的主体.浮游动物群落结构和环境因子的典型对应分析表明,水温、pH、水深、总磷、化学需氧量和溶解氧是与西凉湖浮游动物群落结构相关性较强的环境因子.冬季浮游动物群落结构与总磷、化学需氧量和溶解氧呈显著正相关;春季、夏季、秋季三个季节的浮游动物群落结构与pH、水温和水深呈显著正相关,表明西凉湖浮游动物群落结构的季节变化与水温及营养状况密切相关.

关键词：浮游动物群落结构水质西凉湖

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停用氧化塘的污染现状

应用与环境生物学报 2005年第2期11卷 175-178页

作者：冯伟松范晓鹏缪炜沈韫芬梁勇徐盈中国科学院水生生物研究所武汉430072 华中科技大学环境学院武汉430074

根据国家标准《水质——微型生物群落监测——PFU法》(GB/T12990 -91),两次对湖北鸭儿湖生物氧化塘进行了生物监测与评价.共检出原生动物192种,其中植鞭毛虫48种、动鞭毛虫46种、纤毛虫82种、肉足虫16种.试验所得生物学参数,即PFU原生... 详细信息

根据国家标准《水质——微型生物群落监测——PFU法》(GB/T12990 -91),两次对湖北鸭儿湖生物氧化塘进行了生物监测与评价.共检出原生动物192种,其中植鞭毛虫48种、动鞭毛虫46种、纤毛虫82种、肉足虫16种.试验所得生物学参数,即PFU原生动物群落结构和功能参数表明:如今Ⅰ号氧化塘整体状况与1982年相比变化较大,Ⅱ号氧化塘相比1982年也有所变化,但不是很大.其它3个氧化塘,第一次试验结果为从Ⅲ号氧化塘到Ⅴ号氧化塘依次变好;第二次试验结果则按Ⅴ、Ⅲ、Ⅳ顺序变好.两次试验所得此三塘的生物学参数之间差别并不大.鸭儿湖氧化塘水体状况与1982年所得结果相比有了很大改善.

关键词： PFU法原生动物群落生物监测氧化塘