检索结果-南通市图书馆

Mesenchymal stem cell sheet transplantation combined with locally released simvastatin enhances bone formation in a rat tibia osteotomy model

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mesenchymal stem cell sheet transplantation combined with lo...

2012年浙江省骨科学术年会

作者： Jianwei Wang Yiying Q Tengfei Zhao weiqi yan Kan Xu Zhongli Shi Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University

Nonunion of fractured bones is a common clinical problem for orthopedic surgeons. This study aimed to investigate the effects of simvastatin locally applied from calcium sulphate (CS) combined with mesenchymal stem cell (MSC) sheet on fracture healing. In vitro, the proliferation and differentiation of rat bone marrow derived MSCs stimulated by simvastatin were investigated. In vivo, an osteotomy model was made in rat tibia and fractured tibias were treated with CS, CS/simvastatin, CS/MSC sheet or simvastatin-loaded CS with MSC or untreated (control). Tibias were harvested at 2 or 8 weeks and underwent RT-PCR, X-ray, micro-CT and histological analysis. The expression levels of BMP-2, ALP, OCN, OPG and VEGF of simvastatin-induced MSCs increased with the concentrations of the simvastatin, significantly higher than those in the MSCs group. At 2 weeks, the CS/simvastatin/MSC sheet group showed significantly higher expressions of BMP-2, ALP, OCN, OPG and VEGF;more callus formation around the fracture site compared with other four groups. At 8 weeks, complete bone union was obtained in the CS/simvastatin/MSC sheet group. By contrast, new regenerated bone tissue partially bridged the gap in the CS/simvastatin group and CS/MSC sheet group;the control and CS group showed nonunion of the tibia. These results showed that both simvastatin and MSC sheet contributed to the formation of new bone and the tibia fracture was completely cured by transplantation of MSC sheet with locally applied simvastatin. Such MSC sheet with locally applied simvastatin may contribute to the treatment of fractures, nonunion bone defects in clinic.

关键词： simvastatin mesenchymal stem cells MSC sheet bone formation nonunion

Continuous wave ultrasound enhances vancomycin release and antimicrobial efficacy of antibiotic-loaded acrylic bone cement in vitro and in vivo

在线全文

cnki会议

学校读者我要写书评

暂无评论

Continuous wave ultrasound enhances vancomycin release and a...

2006年浙江省骨科学术会议暨浙江省脊柱脊髓学术会议

作者： Xunzi Cai, Shigui yan, weiqi yan, Xuesong Dai, Haobo Wu Department of Orthopaedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University (310009 )

Although antibiotic-loaded bone cement (ALBC) is used as a drug delivery vehicle to decrease infection rates, the varied clinical effect of the antibiotic release remains controversial. The objective of this study is to investigate the evhancement of continuous wave ultrasound (CWU) on vancomycin release and antimicrobial efficacy of ALBC in vitro and in vivo. We measured vancomycin concentrations after a 0.5-h exposure of CWU. The results showed that CWU increased the drug elution by 2.57%-27.44% compared with the controls in vutro. Ultrasonic intensity and vancomycin load both had a significant effect on the cumulative drug elution at 10.5h, with a significant interaction between each other. We also implanted ALBC specimens into hip joints of sixteen New Zealand White female rabbits after inoculations of Staphylococcus aureus around primary implants for 30d. vancomycin concentrations in the hip cavity and urinary elimination of vancomycin were both measured after intermittent exposures of CWU. The results showed that CWU increased local Tmax by 47.6 μg/mL and urinary elimination of vancomycin by 109.56μg, but failed to prolong local T>MIC. On day 30 after the implantation, assessment in clinical performance, radiology, bacteriology and histology all showed a tendency of decreased bacterial vitality and relieved inflammation in the infected hip treated by CWU. This study suggested that CWU could effectively enhance vancomycin release and antimicrobial efficacy of ALBC, which may be of clinical significance for treating prosthesis-related infections.

关键词：

Biological efficacy of hydroxyapatite/plla immobilized by urease on osteoblast-like cells

在线全文

cnki会议

学校读者我要写书评

暂无评论

Biological efficacy of hydroxyapatite/plla immobilized by ur...

2006年浙江省骨科学术会议暨浙江省脊柱脊髓学术会议

作者： Xin Huang, Disheng yang, weiqi yan Dept. of Orthopaedic Surgery, The Second Affiliated Hospital, Medical College, Zhejiang University (310009)

Aim Polymer composites reinforced by bioactive ceramics and glasses have been proposed as a novel class of bioactive *** precipitate hydroxyapatite(HA)on the 3D polymer surface in only few days,We used immobilized urease method to incorporate HA onto the polymer Poly L-lactic Acid (PLLA)and evaluated their effects on cell growth and differentiation with osteoblastic-like cell model． Materials and Methods Osteoblastic-like MG63 cells were seeded on the HA／PLLA and PLLA(as

关键词：

铜催化草酸二甲酯加氢副产物1,2-丙二醇生成机理的DFT研究

在线全文

cnki会议

学校读者我要写书评

暂无评论

燃料化学学报（中英文） 2024年第4期52卷 553-564页

作者：伦国栋严伟琦周静红朱贻安李伟华东理工大学化学工程联合国家重点实验室上海200237 华东理工大学化工学院上海200237

利用密度泛函理论对Cu(111)及Cu_(2)O(111)表面上草酸二甲酯加氢副产物1,2-丙二醇(1,2-PDO)的生成机理进行了探究,计算了两种表面上1,2-PDO生成的不同反应路径基元步骤的热力学数据以及所涉及物种的吸附行为,进行了局域态密度以及差分... 详细信息

利用密度泛函理论对Cu(111)及Cu_(2)O(111)表面上草酸二甲酯加氢副产物1,2-丙二醇(1,2-PDO)的生成机理进行了探究,计算了两种表面上1,2-PDO生成的不同反应路径基元步骤的热力学数据以及所涉及物种的吸附行为,进行了局域态密度以及差分电荷密度分析,阐明了铜催化剂的主要活性位点及1,2-PDO生成的主要路径。结果表明,1,2-PDO主要由乙二醇和甲醇于Cu_(2)O(111)表面通过Guerbet醇缩合反应生成,具体包括醇脱氢、羟醛缩合以及不饱和醛加氢三个过程。Cu_(2)O(111)表面Cu_(us)^(+)及O_(suf)^(-)位点形成的Lewis酸碱对能够促进反应物、产物及反应中间体的吸附且对于1,2-PDO生成过程的整体催化活性更高。Cu_(2)O(111)表面的O_(suf)^(-)位点是醇类脱氢生成醛、羟醛缩合过程中生成烯醇物种以及不饱和醛类中间体加氢的主要活性中心,而C-C偶联反应则发生在Cu_(us)^(+)金属位点上。论文研究结果可为铜催化剂设计和改性以及草酸酯加氢工艺的优化提供理论指导。

关键词：草酸二甲酯加氢 Cu催化剂 1,2-丙二醇密度泛函理论反应机理

Effect of Woven Fabric Biodegradable Polyglycolic Acid on Joint Resurfacing with Fresh Chondrocytes in a Rabbit Model

在线全文

学校读者我要写书评

暂无评论

Effect of Woven Fabric Biodegradable Polyglycolic Acid on Jo...

2009年浙江省骨科学学术年会

作者： Orthopaedic Research Center The 2nd Affiliated Hospital of Medical School Zhejiang University Hangzhou China Zhongli Shi weiqi yan Jie Feng Binggang Guan yangbo Liu Shigui yan

To evaluate the effectiveness of the cell-material in situ on joint resurfacing,a woven fabric polyglycolic acid(PGA) treated with fresh chondrocytes was used for repairing cartilage ***-thickness defects were created in the weight-bearing surfaces of the femoral intercondylar fossa in a rabbit model. The defect was filled with and without PGA under surgical *** implantation,chondrocytes were co-cultured with PGA for one *** animals were sacrificed at eight weeks after implantation and evaluated grossly and histological *** examination showed that for PGA/chondrocytes group,the repaired tissue appeared similar in color and texture to the surrounding articular *** for the untreated control,no cartilage-like tissue was observed at all defects,but connective fibrous *** analysis revealed neochondrogenesis and clusters of cartilage matrix with specific safranin-O staining for the PGA/cell *** Gross and histological evaluation indicated a significantly higher score for PGA/cell group than for PGA and control *** results suggest that the woven fabric PGA may facilitate the formation of cartilage tissues by providing a biodegradable and good-handle vehicle for the delivery to and retention of organized cell matrix constructs in vivo *** might therefore enhance neochondrogenesis because of the superior biodegradable and biocompatible of PGA scaffold sheet,while the more suitable biological environment might sustain cell growth and in situ cell function,suggesting a promising candidate for functional tissue engineering of clinical environment.

关键词：

在线全文

cnki会议

学校读者我要写书评

暂无评论

台州某电镀公司废水处理工程设计

广东化工 2023年第20期50卷 83-87页

作者：阎伟琦浙江伟星实业发展股份有限公司浙江临海317000

台州某电镀公司生产过程中产生槽液、清洗废水、含镍、含铬等废水,种类繁多,且水量不统一,处理难度较大。企业结合水质特点及实际情况采用分类收集+预处理+混凝絮凝工艺,再分别对每股废水做进一步处理后,调节废水pH进行生化好氧步骤,出... 详细信息

台州某电镀公司生产过程中产生槽液、清洗废水、含镍、含铬等废水,种类繁多,且水量不统一,处理难度较大。企业结合水质特点及实际情况采用分类收集+预处理+混凝絮凝工艺,再分别对每股废水做进一步处理后,调节废水pH进行生化好氧步骤,出水水质达到相关要求。

关键词：电镀废水混凝絮凝好氧工程设计

股前外侧血流桥接嵌合穿支皮瓣游离移植治疗伴主干动脉缺损的上肢复合组织缺损的临床效果

在线全文

学校读者我要写书评

暂无评论

中华烧伤与创面修复杂志 2024年第2期40卷 172-179页

作者：刘飞闫炜琪马强刘亦斌杨智斌宁夏医科大学总医院手足踝外科银川750001 宁夏医科大学总医院烧伤整形美容科银川750001 陆军军医大学(第三军医大学)第二附属医院整形美容科重庆400037

目的探讨股前外侧血流桥接嵌合穿支皮瓣游离移植治疗伴主干动脉缺损的上肢复合组织缺损的临床效果。方法该研究为回顾性观察性研究。2019年5月—2022年1月,宁夏医科大学总医院手足踝外科收治11例符合入选标准的伴主干动脉缺损的上肢复... 详细信息

目的探讨股前外侧血流桥接嵌合穿支皮瓣游离移植治疗伴主干动脉缺损的上肢复合组织缺损的临床效果。方法该研究为回顾性观察性研究。2019年5月—2022年1月,宁夏医科大学总医院手足踝外科收治11例符合入选标准的伴主干动脉缺损的上肢复合组织缺损患者,其中男7例、女4例,年龄18~56岁。清创后,皮肤软组织缺损面积为20 cm×6 cm~32 cm×10 cm,无效腔或深部组织裸露面积为7 cm×4 cm~10 cm×7 cm。3例患者桡动脉缺损,长度为4~7 cm;2例患者尺动脉缺损,长度为5~8 cm;4例患者尺、桡动脉均缺损,长度为3~7 cm;2例患者尺、桡动脉及肱动脉均缺损,长度为4~8 cm。设计并切取股前外侧血流桥接嵌合穿支皮瓣,切取皮瓣面积为22 cm×7 cm~32 cm×11 cm、嵌合肌瓣面积为7 cm×4 cm~10 cm×7 cm,“T”形血管蒂中桥接血管的长度为4~8 cm。移植皮瓣时,将血管蒂近端同受区血管近端吻合、血管蒂远端同前臂远端较正常的血管吻合,利用肌瓣填塞无效腔。对组织瓣供区创面采用直接闭合或移植皮片处理。术后,观察皮瓣血运及成活情况、远端肢体成活情况、移植皮片成活情况。术后2~4周,行CT血管造影(CTA)检查,观察吻合动脉近远端的通畅情况。随访时观察皮瓣质地、供区创面愈合情况。结果1例患者(前臂完全离断)于术后第5天出现肢体远端血运障碍,CTA检查提示桥接的动脉和皮瓣远端吻合口动脉均栓塞,急诊探查显示远端肢体肌肉及皮肤软组织坏死较多,遂行截肢术;其余10例患者皮瓣均未发生血管危象且全部成活,远端肢体全部成活,供区移植皮片成活良好。术后2~4周,吻合的动脉近远端通畅情况良好。随访11~37个月,皮瓣质地良好,所有供区创面愈合良好。结论运用股前外侧血流桥接嵌合穿支皮瓣修复伴主干动脉缺损的上肢复合组织缺损可提高保肢成功率,值得在临床推广。

关键词：显微外科手术穿支皮瓣上肢股前外侧皮瓣血流桥接皮瓣嵌合皮瓣创面修复

Repair of full-thickness articular cartilage defects using woven fabric tissue engineered cartilage in rabbits

在线全文

学校读者我要写书评

暂无评论

Repair of full-thickness articular cartilage defects using w...

2006年浙江省骨科学术会议暨浙江省脊柱脊髓学术会议

作者： Zhongli Shi, weiqi yan, Jie Feng, Binggang Guan, yangbo Liu Orthopaedic Research Center, The 2nd Affiliated Hospital of Medical School, Zhejiang University (310009)

Aims The repair and regeneration of damaged articular cartilage remains one of the most challenging problems in ***,interest has focused on the transplantation of chondrogenic cells to repair cartilage defects,however the appropriate cell in scaffold for neocartilage tissue is still *** this study,a woven fabric PGA(polyglycolic acid)combined with chondrocytes or MSCs (mesenchymal stem cells)was used for repairing full cartilage defects．-The effectiveness of the cell-material composite on joint resurfacing was evalnated in rabbit *** and Methods A

关键词：

急性闭合性跟腱断裂经皮微创修复术后关节僵硬与踝关节功能预后的危险因素分析

在线全文

cnki会议

学校读者我要写书评

暂无评论

中华骨与关节外科杂志 2023年第12期16卷 1094-1099页

作者：张宁刘亦斌闫玮琪康志学刘飞宁夏医科大学总医院手足踝外科银川750004

目的:分析急性闭合性跟腱断裂经皮微创修复术后关节僵硬与踝关节功能预后的危险因素。方法:回顾性分析2019年1月至2022年1月接受经皮微创修复术治疗的120例急性闭合性跟腱断裂患者的病例资料,根据Arner-Lindholm评定法评估术后预后情况... 详细信息

目的:分析急性闭合性跟腱断裂经皮微创修复术后关节僵硬与踝关节功能预后的危险因素。方法:回顾性分析2019年1月至2022年1月接受经皮微创修复术治疗的120例急性闭合性跟腱断裂患者的病例资料,根据Arner-Lindholm评定法评估术后预后情况,将其分为预后良好组(90例)和预后不良组(30例),探究急性闭合性跟腱断裂患者术后关节僵硬与踝关节功能改善情况。应用单因素分析及多因素logistic回归分析探究急性闭合性跟腱断裂患者术后预后情况的主要危险因素。结果:单因素分析显示急性闭合性跟腱断裂患者术后预后不良情况发生与性别、年龄、断裂位置、损伤范围、踝关节积液情况无关(P>0.05);主要与断端间隙、伤口感染、并发踝关节骨折、术后外固定时间、术后负重时间、延迟治疗、腓肠神经损伤、再断裂的情况有关(P<0.05)。以急性闭合性跟腱断裂患者术后预后情况为因变量,选取单因素分析中P<0.05的变量作为自变量,行多因素logistic回归分析,结果发现,并发踝关节骨折、断端间隙、延迟治疗、伤口感染、再断裂、术后负重时间、腓肠神经损伤、术后外固定时间为急性闭合性跟腱断裂患者术后发生预后不良情况的主要因素(P<0.05)。与术后1个月相比,术后3个月患者的踝关节活动度、踝关节跖屈肌力、跟腱长度、跟腱完全断裂评分(ATRS)、美国足踝外科协会(AOFAS)踝-后足评分有所提升;与术后3个月相比,术后7个月患者的踝关节活动度、跟腱长度、踝关节跖屈肌力、AOFAS评分、ATRS评分显著上升(P<0.05)。结论:通过经皮微创修复术对急性闭合性跟腱断裂进行干预,可有效改善患者踝关节功能,缓解跟腱活动度。并发踝关节骨折、延迟治疗、腓肠神经损伤、断端间隙、伤口感染、再断裂、术后负重及外固定时间为患者预后不良情况发生的主要危险因素。

关键词：急性闭合性跟腱断裂经皮微创修复术关节僵硬踝关节功能