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Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis

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Plant Diversity 2018年第6期40卷 292-298页

作者： Yanxia Jia weiqi li Germplasm Bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences

Storage oil breakdown plays an important role in the life cycle of many plants by providing the carbon skeletons that support seedling growth immediately following germination. 1-Butanol, a specific inhibitor of phospholipase D(PLD)-dependent production of the signalling molecule phosphatidic acid(PA), inhibited Arabidopsis seed germination. N-Acylethanolamines(NAEs), which have been shown to inhibits PLDa1 activity, have no effect on seed germination. However, mobilization profile of triacylglycerols(TAG) that induced by each compound has not been reported. To gain deeper insights into the mode of mobilization of TAG during NAE 12:0 or 1-butanol treatment, we conducted a detailed comparative analysis of the effect of NAE 12:0, DMSO, 1-butanol and tert-butanol on Arabidopsis seed germination and fatty acid composition, tert-butanol and DMSO served as the corresponding controls treatment respectively. Our data show that 1-butanol, but not the inactive tert-butanol isomer, inhibited Arabidopsis seed germination, which is accompanied by a with retardation of the mobilization of triacylglycerols(TAG). In contrast, NAE 12:0 did not affect mobilization of TAG, nor did it significantly delay seed germination as monitored by radicle and cotyledon emergence. 1-Butanol induced RNA degradation in seeds and seedlings. We speculate that the large-scale degradation of RNA under the induction of 1-butanol may lead to abnormal gene expression in genes necessary for seed germination, including the genes needed for the mobilization of oil bodies, and thus cause a delay of seed germination. To the best of our knowledge, we report for the first time that 1-butanol delays the mobilization of TAG.

关键词： Germination 1-butanol N-Acylethanolamines Triacylglycerols Fatty acid

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Flavonoid scutellarin positively regulates root length through NUTCRACKER

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Plant Diversity 2021年第3期43卷 248-254页

作者： Xing Huang weiqi li Xudong Zhang Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunming650201China University of the Chinese Academy of Sciences Beijing100049China

Exploring approaches to regulate meristem is of special importance and broad *** this study,we found that the flavonoid scutellarin,which has a 6-hydroxyl and a 7-glucoside,increased root length through the transcription factor NUTCRACKER(NUC).This root lengthening disappeared in NUCknockout and reappeared in NUC-rescue *** induced NUC expression and promoted the division of cortex/endodermal *** contrast,naringenin,which has same chemical backbone but without 6-hydroxyl and with 7-hydroxyl group,showed the opposite or no *** results demonstrate that scutellarin promotes root length through NUC-mediated regulatory pathways and reveal that flavonoids with and without the 6-hydroxyl and 7-glucoside have positive and negative effects on meristem size,respectively。

关键词： Root length Meristem size Flavonoid Scutellarin Naringenin NUTCRACKER

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Characterisation of manganese toxicity tolerance in Arabis paniculata

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Plant Diversity 2021年第2期43卷 163-172页

作者： Ting Tang Faqing Tao weiqi li School of life Sciences Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated UtilizationKey Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted SoilsHunan University of Science and TechnologyXiangtan411201China Plant Germplasm and Genomics Center Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunming650201China

Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food *** paniculata is a pioneer species of Brassicaceae found in mining areas,and has the ability to accumulate heavy ***,little is known about the genetic mechanisms of Mn tolerance in *** this study,we found that Mn tolerance and ability to accumulate Mn were higher in *** than in Arabidopsis *** mechanisms underlying the response and recovery of *** to Mn toxicity were further investigated using transcriptome analysis.A total of 69,862,281 base pair clean reads were assembled into 61,627 high-quality unigenes,of which 41,591(67.5%)and 39,297(63.8%)were aligned in the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO),*** response to Mn toxicity,genes were expressed in twelve distinct patterns,which can be divided into four general categories:initial,stable,dose-dependent,and *** that were differentially expressed during Mn response and recovery belong to several dominant KEGG *** early response to Mn toxicity in *** includes the upregulation of genes involved in glutathione ***-binding cassette(ABC)transporter proteins were up-regulated during the entire response phase,and genes involved in glycerophospholipid metabolism were upregulated during the late phase of the Mn *** in the phenylpropanoid pathway were differentially expressed in the repair process after Mn *** findings reveal ideal material and genetic resources for phytoremediation in Mn-contaminated areas and highlight new knowledge and theoretical perspectives on the mechanisms of Mn tolerance.

关键词： Arabis paniculata Manganese toxicity Transcriptome Gene expression

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Impacts of CO2 elevation on the physiology and seed quality of soybean

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Plant Diversity 2020年第1期42卷 44-51页

作者： Guowei Zheng Jia Chen weiqi li College of Chinese Material Medica Yunnan University of Chinese MedicineKunmingYunnanPeople's Republic of China Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnanPeople's Republic of China Yunnan Key Laboratory of Dai and Yi Medicines Yunnan University of Chinese MedicineKunmingYunnanPeople's Republic of China

Understanding the responses of crops to elevated atmospheric carbon dioxide concentrations(E[CO2])is very important in terms of global food *** present study investigates the effects of CO2 enrichment(to 800μmol mol^-1)on the physiology of soybean plants and the nutritional value of their seeds under growth chamber *** photosynthesis of soybean was significantly promoted by E[CO2]at all growth stages,but leaf area and specific leaf weight were not *** levels of mineral elements in the leaves decreased under *** soil properties after soybean cultivation under E[CO2]were not affected,except for a decrease in available ***,the levels of soluble sugars in the seeds were not affected by E[CO2].but the levels of natural antioxidants *** addition,the level of oleic acid decreased under E[CO2].However,levels of fatty acid peroxidation and saturation were *** conclusion.E[CO2] appears to have positive effects on the growth of cultivated soybean plants,but its influence on the nutritional values of soybean seeds is ***?2019 Kunming Institute of *** Academy of Sciences.

关键词： CO2 elevation Isoflavone Tocopherol Fatty acid Mineral element

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Electronic and Spectroscopic Properties of La2@C112 Isomers

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Chemical Research in Chinese Universities 2018年第2期34卷 241-246页

作者： WANG Mingqian WANG Boning li weiqi ZHOU Xin YANG li TIAN Weiquan Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage Ministry of Industry and Information School of Chemistry and Chemical Engineering Harbin Institute of Technology Harbin 150080 P. R. China Department of Physics Harbin Institute of Technology Harbin 150001 P. R. China College of Chemistry and Chemical Engineering Chongqing University Chongqing 401331 P. R. China

Among the 3352 isolated pentagon rule（IPR） isomers and 129073 non-IPR isomers satisfying adjacent pentagon pairs（APPs）≤2 of fullerene C112, the lowest-energy IPR and non-IPR isomers of C112 and C112^6- have been fully screened by the density functional tight-binding（DFTB） and density functional theory（DFT） methods for stu- dying the electronic and spectroscopic properties of La2@C112. The structural features and infrared and absorption spectra of those isomers were analyzed in detail, and the characteristic fingerprint absorption peaks were assigned. To clarify the relative stabilities of La2@C112 isomers at high temperature, entropy contributions were determined at the B3LYP level. IPR isomer La2@C112（C2：860136） is not the lowest-energy isomer but is one of the most important isomers. This is the first work that considers non-IPR C112 isomers when exploring the structure and properties of La2@C112.

关键词： C112 fullerene La-endohedral metallofullerene(La-EMF) Thermostability IR spectnan UV-Vis spectrum

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Graphdiyne oxide substrate-enhanced peroxidase-mimicking performance of Ru nanoparticles with physiological pH preference

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Nano Research 2024年第3期17卷 1123-1131页

作者： Cong Xu Wenjie Ma Haozhi Wang Leihou Shao weiqi li Ping Yu Lanqun Mao Beijing National Laboratory for Molecular Science Key Laboratory of Analytical Chemistry for Living BiosystemsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Marine Resource Utilization in South China Sea School of Materials Science and EngineeringHainan UniversityHaikou 570228China College of Chemistry Beijing Normal UniversityBeijing 100875China

Modulating electronic structure of metal nanoparticles via metal–support interaction has attracted intense interest in the field of catalytic ***,the roles of supporting substrates in regulating catalytic properties of nanozymes remain *** this study,we find that the use of graphdiyne oxide(GDYO)as the substrate for self-terminating growth of Ru nanoparticles(Ru@GDYO)endows the peroxidase-like activity of Ru nanoparticles with intrinsic physiological pH preference and natural horseradish peroxidase(HRP)comparable *** nanoparticles electrolessly deposited onto GDYO possess a partially oxidized electronic structure owing to limited charge transfer between Ru and GDYO,contributing to the intrinsic physiological pH preference of the peroxidase-mimicking *** importantly,the substrate GDYO plays an influential factor in enhancing catalytic activity,that is,the activity of Ru@GDYO is much higher than that of Ru nanoparticles deposited on other carbon substrates including graphene oxides and *** demonstrate the application of Ru@GDYO nanozyme in neutral solutions,we employ Ru@GDYO with nicotinamide adenine dinucleotide(NAD+)-dependent dehydrogenases in physiological conditions to realize a sustainable cascade reaction by means of forming continuous NAD^(+)/dihydronicotiamide adenine dinucleotide(NADH)*** finding represents a promising perspective on designing high-performance peroxidase-mimicking nanozymes with broader applicability,raising fundamental understanding of structure–activity relationship,and investigating new applications of nanozymes in biological systems.

关键词： nanozyme graphdiyne reactive oxygen species electroless deposition metal–support interaction

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Theoretical Studies on the Electronic Structure of Nano-graphenes for Applications in Nonlinear Optics

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Chemical Research in Chinese Universities 2022年第2期38卷 579-587页

作者： CHEN Kaichun ZHENG Xuelian YANG Cuicui TIAN Wei Quan li weiqi YANG ling Chongqing Key Laboratory of Theoretical and Computational Chemistry College of Chemistry and Chemical EngineeringChongqing UniversityHuxi CampusChongqing 401331P.R.China School of Physics Harbin Institute of TechnologyHarbin 150001P.R.China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006P.R.China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage Institute of Theoretical and Simulational ChemistrySchool of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001P.R.China

In this work,azulene is introduced into nano-graphene with coronene center to enhance the second-order nonlinear optical(NLO)*** sum-over-states(SOS)model based calculations demonstrate that dipolar contributions are larger than octupolar contributions to the static first hyperpolarizability(〈β0〉)in most nano-graphenes except those with high symmetry(e.g.,a C2v nano-graphene has octupolar contributionsΦJ=3 up to 59.0%of the〈β0〉).Nano-graphenes containing two parallel orientating azulenes(i.e.,Out-P and Out-Ps)have large dipole moments,while their ground state is *** B/N/BN atoms into the positions with a high spin density transfers the ground state of Out-P and Out-Ps to closed-shell singlet,and the Out-Ps-2N has a large〈β0〉of 1621.67×10−30 *** addition of an electron donor(NH2)at the pentagon end enhances the〈β0〉to 1906.22×10−30 *** two-dimensional second-order NLO spectra predicted by using the SOS model find strong sum frequency generations and difference frequency generations,especially in the near-infrared and visible *** strategies to stabilize the electronic structure and improve the NLO properties of azulene-defect carbon nanomaterials are proposed,and those strategies to engineer nano-graphenes to be semiconducting while maintaining theπ-framework are exten-dable to other similar systems.

关键词： Nonlinear optics Azulene-defect nano-graphene Two-dimensional second-order nonlinear optical spectrum Sum frequency generation Difference frequency generation

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Highly sensitive gas sensing material for polar gas molecule based on Janus group-Ⅲ chalcogenide monolayers: A first-principles investigation

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Science China(Technological Sciences) 2020年第8期63卷 1566-1576页

作者： PANG KaiJuan WEI YaDong li weiqi ZHOU Xin JIANG YingJie YANG JianQun li XingJi GAO Lang JIANG YongYuan School of Physics Harbin Institute of TechnologyHarbin 150001China School of Chemistry and Chemical Engineering Harbin Institute of TechnologyHarbin 150001China School of Materials Science and Engineering Harbin University of Science and TechnologyHarbin 150080China School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001China

Gas sensors play an indispensable role in industrial,personal safety and environmental protection,and have been widely investigated by *** some cases,the sensitivity of a two-dimensional material sensor can be enhanced by generating vacancies or applying an external electric *** results can be achieved using Janus 2D materials,which have an inherent electric field and are good candidates for high-sensitivity gas *** this study,the electronic and transport properties of Janus group-Ⅲchalcogenide monolayers(Ga2SSe,In2SSe)were investigated for detecting CO2 and NO2 using first-principles *** several parameters that affect the performance of gas sensors,such as adsorption distances,adsorption energies,charge transfers and density of states(DOS),a detailed comparison of Janus group-Ⅲchalcogenide monolayers has been provided with their pristine systems,Janus group-Ⅲchalcogenide monolayers(Ga2SSe,In2SSe)have high *** was mainly ascribed to the built-in electric field caused by the out-of-plane asymmetric structure of Janus monolayer which enhances the dipole-dipole interaction between the polar gas molecule and the 2D *** the variation of transmission spectra of the Janus group-Ⅲchalcogenide monolayer before and after adsorbing molecules further proves the feasibility of this kind of material as a high-sensitivity gas sensor.

关键词： group-III chalcogenide monolayers gas sensors transmission spectrum electronic structure first-principles calculations

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Fluidic memristor:Bringing chemistry to neuromorphic devices

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The Innovation 2023年第3期4卷 102-103页

作者： Tianyi Xiong weiqi li Ping Yu Lanqun Mao Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living BiosystemsInstitute of ChemistryChinese Academy of Sciences(CAS)Beijing 100190China College of Chemistry Beijing Normal UniversityBeijing 100875China University of Chinese Academy of Sciences Beijing 100049China

Neuromorphic devices,devices with resistance switch dynamic emulating the behavior of neurons,are experiencing a surge in development due to the demand for artificial intelligence and therapeutic devices such as neuroprosthetics.1 Toward reproducing the behaviors of neurons with artificial devices,neuromorphic devices based on two-terminalmemristors(devices with a single-valued relationship between charge Q and magnetic flux 4,i.e.,d4=MdQ)with various mechanisms(e.g.,redox,phase change,magnetic tunnelling,and ferroelectricity)have been developed for various applications,like in-memory computing or intelligent sensing.2 However,the performances and functions of these devices still lag behind those of real *** example,biological neurons are strongly responsible for chemical changes in electrolyte solutions induced by transmitters and regulators,while most neuromorphic devices only show responses to the stimulations of electrical *** and responding to these chemical signals could not only improve the performances of neuromorphic computing but also offer brand-new functions in brain-computer ***,the fabrication of neuromorphic devices responsible for chemical information remains a great problem since the founding of the memristor.

关键词： artificial founding chemistry

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Aging process design based on the morphological evolution ofγprecipitates in a 4th generation nickel-based single crystal superalloy

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Journal of Materials Science & Technology 2023年第16期147卷 176-188页

作者： Jiachen Xu Xinbao Zhao weiqi li Hao liu Quanzhao Yue Huanchang Duan Yuefeng Gu Ze Zhang Institute of Superalloys Science and Technology School of Materials Science and EngineeringZhejiang UniversityHangzhou 310027ZhejiangChina Polytechnic Institute Zhejiang UniversityHangzhou 310027ZhejiangChina

In order to meet the design requirements of the aging treatment process of a 4th generation nickel-based single crystal superalloy(Ni-SX)developed independently,the effects of aging temperatures and aging times on the precipitation and morphological evolution ofγprecipitates are *** morphological evolution behavior ofγprecipitates during the aging process is summarized subsequently and the coarsening behavior ofγprecipitates is discussed by comparing with the lifshitz-Slyozov-Wagner model(LSW)and the trans-interface diffusion-controlled model(TIDC).It is demonstrated that primary aging temperature and secondary aging time dominate the size and squareness ofγprecipitates respectively,a narrow primary aging temperature range and a suitable secondary aging time are allowed to obtain the optimized morphology ofγ*** optimal aging process of the Ni-SX investigated in the present work is obtained for 1100-1120°C/4 h and 870°C/16 h,confirmed by the corresponding creep *** coarsening growth ofγprecipitates in short-term aging also conforms to the LSW model ***,the aging process design rules of various Ni-SXs of different generations are also summarized.

关键词： Nickel-based single crystal superalloy Aging temperature Aging time γprecipitates Creep

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