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Effects of different forms of nitrogen addition on microbial extracellular enzyme activity in temperate grassland soil

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Ecological Processes 2022年第1期11卷 483-490页

作者： Lili Dong Björn Berg weiping gu Zhengwen Wang Tao Sun Erguna Forest-Steppe Ecotone Research Station Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China Department of Forest Sciences University of Helsinki00014 HelsinkiFinland Section of Biology University of Gävle80176 GävleSweden Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China

Background:Nitrogen(N)deposition alters litter decomposition and soil carbon(C)sequestration by influencing the microbial community and its enzyme activity.Natural atmospheric N deposition comprises of inorganic N(IN)and organic N(ON)compounds.However,most studies have focused on IN and its effect on soil C cycling,whereas the effect of ON on microbial enzyme activity is poorly understood.Here we studied the effects of different forms of externally supplied N on soil enzyme activities related to decomposition in a temperate steppe.Ammonium nitrate was chosen as IN source,whereas urea and glycine were chosen as ON sources.Different ratios of IN to ON(Control,10:0,7:3,5:5,3:7,and 0:10)were mixed with equal total amounts of N and then used to fertilize the grassland soils for 6 years.Results:Our results show that IN deposition inhibited lignin-degrading enzyme activity,such as phenol oxidase(POX)and peroxidase(PER),which may restrain decomposition and thus induce accumulation of recalcitrant organic C in grassland soils.By contrast,deposition of ON and mixed ON and IN enhanced most of the C-degrading enzyme activities,which may promote the organic matter decomposition in grassland soils.In addition,theβ-N-acetyl-glucosaminidase(NAG)activity was remarkably stimulated by fertilization with both IN and ON,maybe because of the elevated N availability and the lack of N limitation after long-term N fertilization at the grassland site.Meanwhile,differences in soil pH,soil dissolved organic carbon(DOC),and microbial biomass partially explained the differential effects on soil enzyme activity under different forms of N treatments.Conclusions:Our results emphasize the importance of organic N deposition in controlling soil processes,which are regulated by microbial enzyme activities,and may consequently change the ecological effect of N deposition.Thus,more ON deposition may promote the decomposition of soil organic matter thus converting C sequestration in grassland soils into a C source.

关键词： Inorganic N deposition Organic N deposition Soil microbial biomass Microbial enzyme activity Decomposition Grassland

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水泥基材料热湿耦合传输性能

同济大学学报（自然科学版） 2024年第4期52卷 574-581页

作者：闵红光张伟平顾祥林同济大学工程结构性能演化与控制教育部重点实验室上海200092 中冶建筑研究总院(深圳)有限公司广东深圳518055

通过大气环境下水泥基材料热湿耦合传输试验,考察了温湿度梯度对湿传输、温湿度分布的影响。试验结果表明,湿传输随着温湿度梯度的增大而加快,且温度梯度比湿度梯度对湿传输的影响大;温度梯度比湿度梯度对温度分布的影响大;温湿度梯度... 详细信息

通过大气环境下水泥基材料热湿耦合传输试验,考察了温湿度梯度对湿传输、温湿度分布的影响。试验结果表明,湿传输随着温湿度梯度的增大而加快,且温度梯度比湿度梯度对湿传输的影响大;温度梯度比湿度梯度对温度分布的影响大;温湿度梯度对相对湿度分布的影响都比较大。因此,在水泥基材料热湿耦合传输过程中,水泥浆的湿传输比砂浆快,且应考虑热传输对湿传输的影响,但湿传输对热传输的影响可以忽略。计算结果表明,热湿耦合传输模型对水泥基材料具有较好的适用性。

关键词：水泥基材料热湿耦合传输性能耐久性

Artinian Local Rings Whose Annihilating-ideal Graphs Are Star Graphs

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Algebra Colloquium 2015年第1期22卷 73-82页

作者： Houyi Yu Tongsuo Wu weiping gu Department of Mathematics Southwest University Chongqing 400715 China Department of Mathematics Shanghai Jiaotong University Shanghai 200240 China School of Mechanical Electronic & Information EngineeringChongqing College of Humanities Science &: Technology Chongqing 401524 China

In this paper, a necessary and sufficient condition is given for a commutative Artinian local ring whose annihilating-ideal graph is a star graph. Also, a complete char- acterization is established for a finite local ... 详细信息

关键词： Artinian rings local rings quotients of polynomial rings annihilating-ideals,star graphs

Efficacy of biofeedback therapy via a mini wireless device on sleep bruxism contrasted with occlusal splint:a pilot study

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The Journal of Biomedical Research 2015年第2期29卷 160-168页

作者： weiping gu Jie Yang FeiMin Zhang XinMin Yin XiaoLong Wei Chen Wang Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University Department of General Dentistry Affiliated Hospital of StomatologyNanjing Medical University Department of Stomatology Jiangsu province Geriatric InstitutionJiangsu Province Official Hospital Department of Prosthodontics Affiliated Hospital of StomatologyNanjing Medical University Department of Stomatology Benq Medical CenterNanjing Medical University College of Automation Engineering Nanjing University of Aeronautics and Astronautics

The putative causes of bruxism are multifactorial and there are no definite measures for bruxism management.The aim of this study was to evaluate the efficacy of biofeedback therapy on sleep bruxism,compared with occlusal splint.Twenty-four volunteers with sleep bruxism were divided into two groups：the GTB group that were treated with biofeedback therapy（n 512） and the GTO group that were treated with occlusal splint（n 512）.A mini pressure sensor integrated with a monitoring circuit by use of a maxillary biofeedback splint was fabricated.To foster the relaxation of the masticatory muscles and the nervous system,the wireless device received signals from bruxism events and vibrations alerted the bruxer when the threshold was exceeded.Total episodes and average duration of bruxism events during8 hours of sleep were analyzed with the monitoring program（TRMY1.0）.After 6 and 12 weeks,the episodes（P 50.001） and duration（P,0.05） in the GTB group declined dramatically.In contrast,there were no significant differences in the GTO group after the treatment（P.0.05）.Furthermore,the episodes had significant differences between the GTB group and the GTO group after the same period of treatment（P 5 0.000）.The results suggest that biofeedback therapy may be an effective and convenient measure for mild bruxers,when compared with occlusal splint therapy.The mini wireless biofeedback method may be of value for the diagnosis and management of bruxism in the future.

关键词： sleep bruxism biofeedback occlusal splint miniaturization vibration wireless technology

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数字党建提升国有企业党建工作质量的探讨

当代石油石化 2024年第6期32卷 60-62页

作者：顾伟萍中石化上海工程有限公司上海200120

党的领导和党的建设是我国国有企业的优势和特色。在加快推进数字化发展的时代背景下,探讨以数字党建提升国有企业党建工作质量、赋能企业高质量发展的动能及原因,分析推动数字党建落地落实的关键因素,并制定一系列应对策略。通过数字... 详细信息

党的领导和党的建设是我国国有企业的优势和特色。在加快推进数字化发展的时代背景下,探讨以数字党建提升国有企业党建工作质量、赋能企业高质量发展的动能及原因,分析推动数字党建落地落实的关键因素,并制定一系列应对策略。通过数字党建赋能,推动党建和业务工作的深度融合,引领国有企业增强核心功能和核心竞争力。

关键词：数字党建国有企业党的建设数字技术

红细胞沉降率结合hs-CRP水平预测脊柱结核患者预后的临床价值

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中国医学创新 2024年第6期21卷 121-125页

作者：顾伟佴伟萍孙朝花扬州市第三人民医院结核科江苏扬州225002 扬州市第三人民医院呼吸科江苏扬州225002

目的:分析红细胞沉降率(ESR)结合超敏C反应蛋白(hs-CRP)预测脊柱结核患者预后的临床价值。方法:回顾性分析2016年1月—2022年1月于扬州市第三人民医院住院的102例脊柱结核患者的临床资料,同时招募60例于本院体检健康人士分别记为研究组... 详细信息

目的:分析红细胞沉降率(ESR)结合超敏C反应蛋白(hs-CRP)预测脊柱结核患者预后的临床价值。方法:回顾性分析2016年1月—2022年1月于扬州市第三人民医院住院的102例脊柱结核患者的临床资料,同时招募60例于本院体检健康人士分别记为研究组与对照组。比较两组ESR、hs-CRP水平,分析研究组临床资料,采用多因素logistic回归分析影响脊柱结核患者预后的独立因素,以受试者操作特征(ROC)曲线评价ESR、hs-CRP对脊柱结核患者预后的预测价值。结果:研究组ESR、hs-CRP水平均高于对照组(P0.05);ESR、hs-CRP水平比较,差异均有统计学意义(P<0.05)。多因素分析结果显示,ESR、hs-CRP均为影响脊柱结核患者预后的危险因素(P<0.05);ESR、hs-CRP水平联合预测脊柱结核患者预后效果最佳,其曲线下面积(AUC)为0.771,高于单独预测的0.713、0.719(Z=3.526,P=0.027;Z=3.507,P=0.032),敏感度与特异度分别为86.54%、79.02%。结论:脊柱结核患者ESR、hs-CRP水平异常,预后不良患者ESR、hs-CRP水平高于预后良好患者,ESR、hs-CRP水平对脊柱结核患者预后的价值较高。

关键词：红细胞沉降率超敏C反应蛋白脊柱结核敏感度特异度预后良好

超高剂量率FLASH照射降低小鼠肠道正常组织损伤

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中华放射肿瘤学杂志 2024年第2期33卷 152-157页

作者：王伟平古兆琦高强裘家琪王健胡克查皓张福泉中国医学科学院、北京协和医学院、北京协和医院放射治疗科北京100730 清华大学工程物理系北京100084 北京华清加高能电子科技有限公司北京100091

目的研究与常规剂量率照射相比,超高剂量率(FLASH)照射是否可降低小鼠肠道的辐射损伤。方法FLASH照射和常规剂量率照射均使用电子线,剂量率分别为750 Gy/s和0.5 Gy/s。105只小鼠采用简单随机化法随机分组。取21只进行体重观察比较,每组7... 详细信息

目的研究与常规剂量率照射相比,超高剂量率(FLASH)照射是否可降低小鼠肠道的辐射损伤。方法FLASH照射和常规剂量率照射均使用电子线,剂量率分别为750 Gy/s和0.5 Gy/s。105只小鼠采用简单随机化法随机分组。取21只进行体重观察比较,每组7只,用9 Gy FLASH和常规剂量率照射小鼠全腹后,隔日测小鼠体重,与对照组进行三组间比较。取24只进行病理检查,对照组小鼠5只,用12 Gy FLASH(10只)和常规剂量率(9只)照射小鼠全腹后3.5 d,取小鼠肠道做病理切片,HE染色后比较两组小鼠每毫米小肠的再生隐窝数与再生隐窝百分比。另取20只,每组10只,分别用FLASH和常规剂量率照射小鼠全腹后,观察记录小鼠生存情况。各组10只小鼠,进行分次照射观察体重,其中FLASH照射为4.5 Gy×2次,常规剂量率照射为9 Gy×1次。再各取10只小鼠,进行进行分次照射观察生存情况,其中FLASH照射为6 Gy×2次,常规剂量率照射为12 Gy×1次。两次照射间隔1 min。使用EBT3胶片监测FLASH和常规剂量率照射小鼠实际受照射剂量。符合正态分布的变量,以x¯±s表示,组间比较采用独立样本t检验。使用log-rank法比较小鼠生存期组间差异。结果经9 Gy全腹照射后,FLASH组小鼠的体重显著高于常规剂量率组,FLASH组和常规剂量率组小鼠照射后7 d,体重分别为(19.8±0.8)g和(18.0±1.8)g(P=0.036),照射后15 d,体重分别为(22.0±1.0)g和(21.2±0.5)g(P=0.075),照射后第25 d,体重分别为(24.2±1.4)g和(22.0±1.2)g(P=0.012)。经12 Gy全腹照射后,FLASH组和常规剂量率组小鼠的平均生存时间分别为4 d和4.7 d(P=0.029)。经12 Gy全腹照射后,FLASH组和常规剂量率组小鼠肠再生隐窝平均数目为2.9∶1.2个/mm(P=0.041),肠再生隐窝百分比为34.1%∶14.1%。经6 Gy×2次FLASH照射的小鼠生存期长于经12 Gy×1次常规剂量率照射者,经4.5 Gy×2次FLASH照射后,小鼠体重高于经9 Gy×1次常规剂量率照射者。结论FLASH照射后小鼠体重、生存和肠再生隐窝数均高于常规剂量率照射,提示FLASH照射的肠道损伤低于常规剂量率照射。

关键词：放射疗法超高剂量率损伤肠道

Targeted gene panel provides advantages over whole-exome sequencing for diagnosing obesity and diabetes mellitus

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Journal of Molecular Cell Biology 2023年第6期15卷 40-52页

作者： Hairong Yu Haoyong Yu Rong Zhang Danfeng Peng Dandan Yan Yunjuan gu Yuqian Bao weiping Jia Hong Zhang Cheng Hu Shanghai Diabetes Institute Shanghai Key Laboratory of Diabetes MellitusShanghai Clinical Center for DiabetesShanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200233China Department of Endocrinology Affiliated Hospital of Nantong UniversityNantong 226001China Institute for Metabolic Disease Fengxian Central Hospital Affiliated to Southern Medical UniversityShanghai 201499China

A small fraction of patients diagnosed with obesity or diabetes mellitus has an underlying monogenic cause.Here,we constructed a targeted gene panel consisting of 83 genes reported to be causative for monogenic obesity or diabetes.We performed this panel in 481 patients to detect causative variants and compared these results with whole-exome sequencing(WES)data available for 146 of these patients.The coverage of targeted gene panel sequencing was significantly higher than that of WES.The diagnostic yield in patients sequenced by the panel was 32.9%with subsequent WES leading to three additional diagnoses with two novel genes.In total,178 variants in 83 genes were detected in 146 patients by targeted sequencing.Three of the 178 variants were missed by WES,although the WES-only approach had a similar diagnostic yield.For the 335 samples only receiving targeted sequencing,the diagnostic yield was 32.2%.In conclusion,taking into account the lower costs,shorter turnaround time,and higher quality of data,targeted sequencing is a more effective screening method for monogenic obesity and diabetes compared to WES.Therefore,this approach could be routinely established and used as a first-tier test in clinical practice for specific patients.

关键词： molecular diagnosis monogenic diabetes mellitus monogenic obesity targeted panel whole-exome sequencing

新工科建设下环境工程专业碳中和创新人才培养探究

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中国现代教育装备 2024年第11期 75-78页

作者：谭小飞熊炜平周成赟顾岩岭曹嵘晖湖南大学环境科学与工程学院湖南长沙410082 长沙理工大学湖南长沙410082

探讨了新工科建设下的环境工程专业碳中和创新人才培养。分析了新工科建设下环境工程专业碳中和创新人才培养现状和培养目标,针对性地提出了碳中和创新人才培养的措施:一是通过优化课程设置,增强学生对碳中和的认识和理解,提升学生掌握... 详细信息

探讨了新工科建设下的环境工程专业碳中和创新人才培养。分析了新工科建设下环境工程专业碳中和创新人才培养现状和培养目标,针对性地提出了碳中和创新人才培养的措施:一是通过优化课程设置,增强学生对碳中和的认识和理解,提升学生掌握和运用碳中和技术的能力;二是通过多种手段强化科研教学,引导学生在科研中不断提高技术水平和创新能力;三是注重实践教学,让学生在实践中获得技能和经验,提升实际操作和解决问题的能力;四是通过碳中和职业规划指导,帮助学生更好地了解和把握该领域的就业机会和趋势,增强其就业竞争力。

关键词：新工科建设环境工程专业碳中和创新人才人才培养