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Bacillus velezensis JK-XZ8 prevents and controls crown gall disease on Prunus subhirtella by colonizing and inducing resistance

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Journal of Forestry Research 2022年第3期33卷 1019-1031页

作者： Xitang Zhang weiliang kong Xiaoqin Wu Jianren Ye Co-Innovation Center for Sustainable Forestry in Southern China College of ForestryNanjing Forestry UniversityNanjing210037JiangsuPeople’s Republic of China Jiangsu Key Laboratory for Prevention and Management of Invasive Species Nanjing Forestry UniversityNanjing210037JiangsuPeople’s Republic of China

Crown gall of Prunus subhirtella Miq., flowering cherry, is a soil-borne bacterial disease caused by Agrobacterium tumefaciens Smith & Townsend and has caused serious damage to the species in recent years in China. In this study, the colonization of sapling roots, the biocontrol efficacy, the expression of defense-related genes and the enzyme activity in roots of P. subhirtella inoculated with the Bacillus velezensis JK-XZ8 against A. tumefaciens were determined under greenhouse conditions. The results showed that the JK-XZ8 strain colonized in the rhizosphere and root surfaces of the saplings. Sole application of the strain increased activities of polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) without affecting superoxide dismutase (SOD). SOD and PPO activities increased in the prevention group, and the activity of PAL increased in both the prevention and cure groups. The expression of the defense-related genes PAL and PR-1 of salicylic acid pathway in roots increased in both the prevention and cure groups. Simultaneously on day 3, the expression of both COI and MYC2 genes of the jasmonic acid pathway significantly increased in the two groups. The incidence of crown gall in the prevention and the cure groups were 48.1% and 66.7%, respectively, significantly lower compared to the pathogens alone (87.5%). This study showed that the application of the JK-XZ8 strain reduced the incidence of crown gall in P. subhirtella saplings in the two groups, and the prevention group had better control efficacy. In addition, the JK-XZ8 strain protects against crown gall by developing induced systemic resistance and systemic acquired resistance in the roots of the cherry saplings.

关键词： Bacillus velezensis Prunus subhirtella Crown gall Plant-induced resistance Control effect

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Combining ectomycorrhizal fungi and plant growth-promoting rhizobacteria to enhance salt tolerance of Metasequoia glyptostroboides

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Journal of Forestry Research 2023年第5期34卷 1603-1614页

作者： weiliang kong Xiuqian Xu Zhenqian Li Yahui Wang Xiaoqin Wu Co-Innovation Centre for Sustainable Forestry in Southern China College of ForestryNanjing Forestry UniversityNanjing 210037People’s Republic of China Jiangsu Key Laboratory for Prevention and Management of Invasive Species Nanjing Forestry UniversityNanjing 210037People’s Republic of China

Plant growth and productivity are negatively affected by soil *** study investigated the effects of the rhizosphere-promoting bacterium,Bacillus paramycoides JYZ-SD5,and the ectomycorrhizal fungus,Schizophyllum commune Be,on the growth of Metasequoia glyptostroboides under salt *** in biomass,root growth,root ion distribution and in vivo enzyme activities were determined under different treatments(Be,JYZ-SD5,and Be+JYZ-SD5).The results show that all inoculations increased chlorophyll content,shoot length and root diameter with or without salt stress,and the effect of Be+JYZ-SD5was the ***-SD5 and Be+JYZ-SD5 treatments significantly increased root length,surface area,bifurcation number,tip number,main root length and diameter under salt *** chloroplast structures developed under both single and double *** to the control,root activities of *** in the Be,JYZSD5,and Be+JYZ-SD5 treatments increased by 31.3%,17.2%,and 33.7%.All treatments increased the activities of superoxide dismutase(SOD),peroxidase(POD),Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-*** strongest effect was by Be+*** of root ion distribution showed that,under salt stress,Na^(+)and K^(+)decreased and were concentrated in the epidermis or ***/K ratios also *** Be+JYZ-SD5 treatment increased betaine by 130.3%and 97.9%under 50 mM and 100 mM salt stress,***,these changes result in the activation of physiological and biochemical processes involved in the mitigation of salinity-induced stress in ***.

关键词： Bacillus paramycoides Schizophyllum commune Metasequoia glyptostroboides Salt stress Plant growth

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Geometrical and electrical optimization of NS-SDBD streamwise plasma heat knife for aircraft anti-icing

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Chinese Journal of Aeronautics 2023年第2期36卷 87-99页

作者： Zhi SU Hua LIANG Haohua ZONG Jun LI Xu FANG Biao WEI Jie CHEN weiliang kong Science and Technology on Plasma Dynamics Laboratory Air Force Engineering UniversityXi'an 710038China Institute of Aero-engine School of Mechanical EngineeringXi'an Jiaotong UniversityXi'an 710049China Northwest Institute of Nuclear Technology Xi'an 710024China The Green Aerotechnics Research Institute Chongqing Jiaotong UniversityChongqing 404100China

Ice accretion on aircraft encountering supercooled water droplets in clouds poses great risks to flight performance and *** the aim of optimizing the newly developed streamwise plasma heat knife method for anti-icing,a parametric investigation is carried out in this *** influence of the detailed voltage profile on the heating effects of a Surface Dielectric Barrier Discharge driven by Nanosecond Pulses(NS-SDBD)is investigated,and a comparison of the antiicing performance among different configurations of streamwise plasma heat knife is *** results show that columnar high-temperature regions produced by a multi-streamer discharge appear at small pulse rise time,but become diffuse as the pulse rise time *** optimal pulse rise time exists to provide a wide range and high value of temperature,which is found to be 150 ns for the setup in the present *** influence of the pulse fall time is much weaker than that of the rise *** range and value of the temperature decrease with increasing pulse fall time.A greater pulse width is found to improve the heating effect by increasing the discharge *** a spanwise electrode is placed connecting the streamwise electrodes of the streamwise plasma heat knife at the airfoil leading edge,the anti-icing performance becomes poorer,whereas good performance is achieved when the spanwise electrode is at the edge of the streamwise *** on this,a three-level configuration of the plasma heat knife is proposed,and its anti-icing performance is found to be much better than that of the original configuration.

关键词： Airfoil icing mitigation Dielectric barrier discharge Nanosecond pulse Voltage profile Plasma

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Minimizing airfoil drag at low angles of attack with DBD-based turbulent drag reduction methods

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Chinese Journal of Aeronautics 2023年第4期36卷 104-119页

作者： Zhi SU Haohua ZONG Hua LIANG Jun LI Like XIE Xuecheng LIU weiliang kong Borui ZHENG Science and Technology on Plasma Dynamics Laboratory Air Force Engineering UniversityXi’an 710038China Institute of Aero-engine School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710049China The Green Aerotechnics Research Institute of Chongqing Jiaotong University Chongqing 404100China

Dielectric Barrier Discharge(DBD)based turbulent drag reduction methods are used to reduce the total drag on a NACA 0012 airfoil at low angels of *** interaction of DBD with turbulent boundary layer was investigated,based on which the drag reduction experiments were *** results show that unidirectional steady discharge is more effective than oscillating discharge in terms of drag reduction,while steady impinging discharge fails to finish the mission(*** increase).In the best scenario,a maximum relative drag reduction as high as 64%is achieved at the freestream velocity of 5 m/s,and a drag reduction of 13.7%keeps existing at the freestream velocity of 20 m/*** unidirectional discharge,the jet velocity ratio and the dimensionless actuator spacing are the two key parameters affecting the *** drag reduction magnitude varies inversely with the dimensionless spacing,and a threshold value of the dimensionless actuator spac-ing of 540(approximately five times of the low-speed streak spacing)exists,above which the drag *** the jet velocity ratio smaller than 0.05,marginal drag variation is *** con-trast,when the jet velocity ratio larger than 0.05,the experimental data bifurcates,one into the drag increase zone and the other into the drag reduction zone,depending on the value of dimensionless actuator *** both zones,the drag variation magnitude increases with the jet velocity *** total drag reduction can be divided into the reduction in pressure drag and turbulent friction drag,as well as the increase in friction drag brought by transition *** reduction in tur-bulent friction drag plays an important role in the total drag reduction.

关键词： Dielectric barrier discharge Dimensionless scaling Plasma actuator Turbulent friction drag Wake

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Experimental study on dynamic stall control based on AC-DBD actuation

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Plasma Science and Technology 2021年第11期23卷 96-109页

作者： Hesen YANG Hua LIANG Guangyin ZHAO Bo WANG Shengwu ZHANG weiliang kong Science and Technology on Plasma Dynamics Laboratory Air Force Engineering UniversityXi'an 710038People's Republic of China State Key Laboratory of Aerodynamics China Aerodynamics Research and Development CenterMianyang 621000People's Republic of China National Key Laboratory of Rotorcraft Aeromechanics Nanjing University of Aeronautics and AstronauticsNanjing 210016People's Republic of China Institute of Noise&Vibration Naval University of EngineeringWuhan 430030People's Republic of China Academician Workstation Foundation of the Green Aerotechnics Research Institute of Chonging Jiaotong University Chongqing 401120People's Republic of China

To explore AC-DBD's ability in controlling dynamic stall,a practical SC-1095 airfoil of a helicopter was selected,and systematic wind tunnel experiments were carried out through direct aerodynamic *** effectiveness of dynamic stall control under steady and unsteady actuation is *** influence of parameters such as constant actuation voltage,pulsed actuation voltage,pulsed actuation frequency and duty ratio on dynamic stall control effect is studied under the flow condition of k=0.15 above the airfoil,and the corresponding control mechanism is *** actuation can effectively reduce the hysteresis loop area of dynamic lift,and control the peak drag and moment *** unsteady actuation,there is an optimal duty ratio DC=50%,which has the best effect in improving the lift and drag characteristics,and there is a threshold of pulsed actuation voltage in dynamic stall *** optimal dimensionless frequency will not be found;different F+have different control advantages in different aerodynamic coefficients of different pitching *** actuation has obvious control advantages in improving the lift-drag characteristics and hysteresis,while steady actuation can better control the large nose-down moment.

关键词： dynamic stall plasma flow control AC-DBD parameter control law

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Control of flow separation over a wing model with plasma synthetic jets

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Plasma Science and Technology 2022年第6期24卷 135-147页

作者： Zhi SU Guozheng SONG Haohua ZONG Hua LIANG Jun LI Like XIE Xuecheng LIU weiliang kong Science and Technology on Plasma Dynamics Laboratory Air Force Engineering UniversityXi'an 710038People's Republic of China Institute of Aero-Engine School of Mechanical EngineeringXi’an Jiaotong UniversityXi'an 710049People's Republic of China The Green Aerotechnics Research Institute of Chongqing Jiaotong University Chongqing 404100People's Republic of China

An array of 30 plasma synthetic jet actuators(PSJAs)is deployed using a modified multichannel discharge circuit to suppress the flow separation over a straight-wing *** lift and drag of the wing model are measured by a force balance,and the velocity fields over the suction surface are captured by a particle imaging velocimetry *** show that the flow separation of the straight wing originates from the middle of the model and expands towards the wingtips as the angle of attack *** flow separation can be suppressed effectively by the PSJAs *** best flow control effect is achieved at a dimensionless discharge frequency of F^+=1,with the peak lift coefficient increased by 10.5%and the stall angle postponed by 2°.To further optimize the power consumption of the PSJAs,the influence of the density of PSJAs on the flow control effect is investigated.A threshold of the density exits(with the spanwise spacing of PSJAs being 0.2 times of the chord length in the current research),below which the flow control effect starts to deteriorate *** addition,for comparison purposes,a dielectric barrier discharge(DBD)plasma actuator is installed at the same location of the *** the same power consumption,4.9%increase of the peak lift coefficient is achieved by DBD,while that achieved by PSJAs reaches 5.6%.

关键词： flow separation plasma synthetic jet density dielectric barrier discharge

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Research on Intelligent Technology Management and Service Platform

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Journal on Artificial Intelligence 2020年第3期2卷 149-155页

作者： Ning Chen Hui Li Xinxin Fan weiliang kong Yonghong Xie Xinjiang Research Institute of Building Sciences(Co. Ltd.)UrumqiChina School of Automation and Electrical Engineering University of Science and Technology BeijingBeijingChina School of Computer and Communication Engineering University of Science and Technology BeijingBeijingChina Beijing Key Laboratory of Knowledge Engineering for Materials Science BeijingChina

In recent years,there are some problems in science and technology management,such as untimely task supervision and independent information system,which makes it difficult to achieve accurate,quantitative and standardized *** storage of scientific research test data is scattered,and there are many deficiencies in the management,promotion and use of existing intellectual *** this paper,on the basis of the knowledge economy,intelligence economy under the conditions of knowledge management concept and cutting-edge technology,technology management and service management related data,information,knowledge,method of blend *** on science and technology management,knowledge management as the core of management and service platform of science and technology technical route,main function,construction principles and *** order to realize the management systematization of science and technology,intelligent,better educated,multi-level and multi-angle application services.

关键词： Knowledge management demand science and technology management information services

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飞机过冷大水滴结冰气象条件运行设计挑战

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航空学报 2023年第1期44卷 1-15页

作者：陈勇孔维梁刘洪中国商用飞机有限责任公司上海210016 上海交通大学航空航天学院上海200240

过冷大水滴(SLD)环境是一种普遍存在的危险气象,已造成多起空难事故。因其结冰特征异常、导致失事快而受到广泛重视。20余年来,国内外在过冷大水滴结冰机理、模拟方法、试验技术等方面开展了大量研究,但结冰保护和适航取证研究一直进步... 详细信息

过冷大水滴(SLD)环境是一种普遍存在的危险气象,已造成多起空难事故。因其结冰特征异常、导致失事快而受到广泛重视。20余年来,国内外在过冷大水滴结冰机理、模拟方法、试验技术等方面开展了大量研究,但结冰保护和适航取证研究一直进步缓慢。国际最新SLD结冰适航条款的取证是目前中国大型民机面临的一大挑战。更重要的是在严厉的SLD结冰条款要求下飞机结冰条件的运行能力是否提高,这是一直以来缺乏探讨的问题。按基础研究到工程应用的逻辑,依次介绍了过冷大水滴结冰机理、模拟技术、结冰风险评估和防冰方法等方面的研究进展,并对飞机结冰保护设计及运营策略进行了探讨。综述认为飞机SLD结/防冰设计中多个环节技术仍不成熟,包括数值/试验模拟、结冰环境探测和高效防除冰技术,导致SLD结冰安全设计技术风险大、成本高。单纯提升防除冰能力的综合收益低,飞机应重点提升冰环境感知、结冰探测、防除冰精确性和容冰能力。为此需突破精准结冰保护、结冰准确感知和过冷大水滴试验模拟技术,而这有赖于过冷大水滴环境产生方法和溢流结冰机理等基础问题研究的进步。

关键词：过冷大水滴(SLD) 结冰异常性结冰安全防护适航取证运行效率

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拟蕈状芽孢杆菌JYZ-SD5与菌根真菌互作对水杉生长和耐盐性的影响

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林业科学 2023年第1期59卷 99-109页

作者：李振倩吴小芹孔维亮南方现代林业协同创新中心、南京林业大学林学院南京210037

【目的】研究盐胁迫下拟蕈状芽孢杆菌(JYZ-SD5)和裂褶菌(Be)互作对水杉生长和耐盐性的影响,以探究其作用机制,为利用有益微生物提高木本植物耐盐性以及盐碱地生物修复利用提供参考依据。【方法】盐胁迫下采用盆栽试验探讨拟蕈状芽孢杆菌... 详细信息

【目的】研究盐胁迫下拟蕈状芽孢杆菌(JYZ-SD5)和裂褶菌(Be)互作对水杉生长和耐盐性的影响,以探究其作用机制,为利用有益微生物提高木本植物耐盐性以及盐碱地生物修复利用提供参考依据。【方法】盐胁迫下采用盆栽试验探讨拟蕈状芽孢杆菌JYZ-SD5和裂褶菌Be对水杉相关生理代谢指标和耐盐性的影响。【结果】1)与对照相比,各接菌处理均提高了水杉的生物量和根系生长参数,其中双接种JYZ-SD5+Be效果优于单接种,双接种处理的水杉幼苗株高和地径分别提高65.47%和63.46%,根长、根表面积、根体积、总根尖数和分枝数分别显著提高1.05、1.51、2.2、1.88和2.31倍(P<0.05)。2)在盐胁迫下,与未接种对照相比,各接菌处理均有效缓解了盐胁迫对水杉叶片的损害,盐害症状减轻;单接种JYZ-SD5分别显著提高了水杉可溶性糖、可溶性蛋白和脯氨酸12.4%、62.58%和38.99%(P<0.05),单接种Be以及双接种处理分别显著提高水杉脯氨酸122%和35.64%(P<0.05)。3)盐胁迫下,与未接种对照相比,各接菌处理均有效降低水杉H2O2含量、O_(2)·-产生速率、丙二醛含量和电解质渗漏,其中单接种JYZ-SD5分别显著降低26.58%、9%、15.85%和18.76%(P<0.05),单接种Be分别降低8.91%、7.77%、8.54%和22.09%,双接种JYZ-SD5+Be分别显著降低15.58%、26.38%、53.66%和30.39%(P<0.05);同时各接菌处理均显著降低了水杉的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性。4)盐胁迫下,与未接种对照相比,各接菌处理提高了水杉茎和叶中的K+含量,降低了Na^(+)含量,从而降低了Na^(+)/K^(+)比,尤以单接种Be效果最好,分别显著降低水杉茎和叶Na^(+)/K^(+)比23.84%和26.58%(P<0.05)。【结论】根际促生细菌JYZ-SD5与外生菌根真菌Be可促进水杉生长,并可通过提高水杉植株水分吸收、渗透调节物质积累、维持细胞膜稳定性、降低膜脂质过氧化水平、活性氧积累及抗氧化酶活以及降低Na+/K+比来缓解盐胁迫对水杉的损伤,提高水杉对盐胁迫的耐受性。

关键词：拟蕈状芽孢杆菌JYZ-SD5 裂褶菌Be 水杉盐胁迫耐盐性

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壁面表面能影响冰枝尖端生长机制研究

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上海交通大学学报 2018年第8期52卷 918-923页

作者：沈佳瑛孔维梁刘洪上海交通大学航空航天学院上海200240

通过实验研究过冷度和表面能对近绝热表面上壁面冰前缘形貌和生长速度的影响,理论计算了不同壁面接触角的壁面冰生长速度,并与实验结果对比.结果表明:在近绝热壁面上和过冷度小于2K时,冰形态为单冰枝,壁面自由能对结冰速度有明显影响,... 详细信息

通过实验研究过冷度和表面能对近绝热表面上壁面冰前缘形貌和生长速度的影响,理论计算了不同壁面接触角的壁面冰生长速度,并与实验结果对比.结果表明:在近绝热壁面上和过冷度小于2K时,冰形态为单冰枝,壁面自由能对结冰速度有明显影响,但对冰尖端形态改变很小.壁面冰枝前缘尺度和自由冰枝接近,壁面冰枝前缘形状的改变不足以解释生长速度的改变量.说明壁面表面能并非通过传热影响结冰,而是改变冰自身性质.

关键词：过冷水壁面结冰接触角尖端形貌生长速度

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