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Folding and everting distal end of graft flap to reduce orifice stenosis following onlay urethroplasty

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Asian Journal of Andrology 2003年第2期5卷 159-161页

作者： RuiJIANG Jiang-HuaCHEN hong-weili Tong-LiangCHEN KidneyDiseasesCenter FirstAffiliatedHospitalZhejiangUniversityMedicalCollegeHangzhou310003China DepartmentofUrology AffiliatedHospitalLuzhouMedicalCollegeLuzhou646000China

Aim: Orifice stenosis remained to be a common complication of hypospadias repair. We had modified the preputial island flap urethroplasty by folding and everting the distal end of the pedicle graft flap to prevent the neoorifice from stenosis. Methods: Sixteen patients had undergone hypospadias repair using a modified onlay island flap technique. A urethral catheter was retained for 8 days to 10 days after operation. Results: Satisfactory results were seen in all the patients with a cosmetically fine appearance. One patient had a urinary tract infection and another, urethrocutaneous fistula and both were amply treated. No glanular adhesion or stenosis occurred. A long-term follow up of 6 months to 4 years (mean: 2 years) in 15 patients did not find any complication. Conclusion: The modified preputial island flap urethroplasty technique is an easy, reliable and effective approach to reduce orifice stenosis in hypospadias repair.

关键词： hypospadias urethra complications

西藏阿里地区地表温度反演及其影响因素响应特征

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高原科学研究 2024年第2期8卷 14-24页

作者：徐涛洪卫丽杜玉龙鱼静李浩辙陕西地矿区研院有限公司陕西咸阳712000

地表温度是衡量区域生态环境的重要指标之一。西藏自治区阿里地区地处青藏高原西北部,属全球气候变化敏感区,探究阿里地区地表温度的时空分布及相关影响因素,对研究该地区热环境变化过程、监测基础生态变化具有重要意义。文章以2000—2... 详细信息

地表温度是衡量区域生态环境的重要指标之一。西藏自治区阿里地区地处青藏高原西北部,属全球气候变化敏感区,探究阿里地区地表温度的时空分布及相关影响因素,对研究该地区热环境变化过程、监测基础生态变化具有重要意义。文章以2000—2020年间阿里地区TRIMSLST-TP产品为数据源,采用归一化分级方法将LST划分为5个等级,进而采用趋势分析及重心迁移等方法研究阿里地区20年间地表温度时空演变特征,同时选取DEM、NDVI及草地覆盖度3个地表参数,探讨LST影响因子的作用效力。结果表明:(1)阿里地区LST除中温区面积减少外,其余温区面积均有所增加,增加面积由小到大依次为:低温区<次低温区<高温区<次高温区;(2)2000—2020年阿里地区LST年际变化规律为波动下降-迅速上升-小幅下降-缓慢上升,年均温度增长0.06℃;(3)2000—2020年阿里地区低温区、次低温区及中温区重心呈现从东北向西南迁移趋势,次高温区与高温区重心呈现西南向东北方向迁移的趋势;(4)影响因素中,DEM对LST影响为负向,NDVI和草地覆盖度对LST影响为正向。

关键词：地表温度时空演化归一化植被指数时空分布气候变化阿里地区

Personal experience with the procurement of 32 liver allografts

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World Journal of Gastroenterology 2005年第25期11卷 3939-3943页

作者： Guang-WenZhou Cheng-hongPeng hong-weili DepartmentofSurgery RuijinHospitalShanghaiSecondMedicalUniversityShanghai200025China

AIM: To introduce the American Pittsburgh's method of rapid liver procurement under the condition of brain death and factors influencing the quality of donor ***: To analyze 32 cases of allograft liver procurement retrospectively and observe the clinical outcome of orthotopic liver ***: Average age of donors was 38.24±12.78 years,with a male:female ratio of 23:9. The causes of brain death included 21 cases of trauma (65.63%) and nine cases of cerebrovascular accident (28.13%). Fourteen grafts (43.75%) had hepatic arterial anomalies, seven cases only right hepatic arterial anomalies (21.88%), five cases only left hepatic arterial anomalies (15.63%) and two cases of both right and left hepatic arterial anomalies (6.25%) among them. Eight cases (57.14%) of hepatic arterial anomalies required arterial reconstruction prior to transplantation. Of the 32 grafts evaluated for early function, 27 (84.38%) functioned well, whereas three (9.38%) functioned poorly and two (6.25%) failed to function at all. Only one recipient died after transplantation and thirty-one recipients recovered. Four recipients needed retransplantation. The variables associated with less than optimal function of the graft consisted of donor age (35.6±12.9 years vs 54.1±4.3 years, P＜0.05), duration of donor's stay in the intensive care unit (ICU) (3.5±2.4 d vs 7.4±2.1 d, P＜0.005), abnormal graft appearance (19.0% vs 100%, P＜0.05), and such recipient problems as vascular thromboses during or immediately following transplantation (89.3% vs 50.0%, P＜0.005).CONCLUSION: During liver procurement, complete heparization, perfusion in situ with localized low temperature and standard technique procedures are the basis ensuring the quality of the graft. The hepatic arterial anomalies should be taken care of to avoid injury. The donor age,duration of donor's staying in ICU, abnormal graft appearance and recipient problem are important factors influencing the quality of the liver graft.

关键词： Liver transplantation Liver procurement Donor Arterial anomalies

Establishment of a new pig model for auxiliary partial orthotopic liver transplantation

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World Journal of Gastroenterology 2005年第6期11卷 917-921页

作者： Cheng-hongPeng Liu-BinShi hong-WeiZhang Shu-YouPeng Guang-WenZhou hong-weili TransplantationCenter RuijinHospitalShanghai200025China TransplantationCenter HuashanHospitalShanghai200040China DepartmentofGeneralSurgery theSecondAffiliatedHospitalMedicalCollegeZhejiangUniversityHangzhou310009ZhejiangProvinceChina

AIM: To establish a new pig model for auxiliary partial orthotopic liver transplantation (APOLT).METHODS: The liver of the donor was removed from its body. The left lobe of the liver was resected in vivo and the right lobe was used as a graft. After the left lateral lobe of the recipient was resected, end-to-side anastomoses of suprahepatic inferior vena cava and portal vein were performed between the donor and recipient livers,respectively. End-to-end anastomoses were made between hepatic artery of graft and splenic artery of the *** drainage was placed in donor common bile ***: Models of APOLT were established in 5 pigs with a success rate of 80%. Color ultrasound examination showed an increase of blood flow of graft on 5th d compared to the first day after operation. When animals were killed on the 5th d after operation, thrombosis of hepatic vein (HV) and portal vein (PV) were not found. Histopathological examination of liver samples revealed evidence of damage with mild steatosis and sporadic necrotic hepatocytes and focal hepatic lobules structure disorganized in graft. Infiltration of inflammatory cells was mild in portal or central vein area. Hematologic laboratory values and blood chemical findings revealed that compared with group A (before transplantation), mean arterial pressure (MAP), central venous pressure (CVP), buffer base (BB), standard bicarbonate (SB) and K+ in group B (after portal vein was clamped) decreased (P＜0.01). After reperfusion of the graft, MAP, CVP and K+ restored ***: Significant decrease of congestion in portal vein and shortened blocking time were obtained because of the application of in vitro veno-venous bypass during complete vascular clamping. This new procedure,with such advantages as simple vessel processing, quality anastomosis, less postoperative hemorrhage and higher success rate, effectively prevents ischemia reperfusion injury of the host liver and deserves to be spread.

关键词： Auxiliary partial orthotopic liver transplantation Model pig

Protective effect of doxorubicin induced heat shock protein 72 on cold preservation injury of rat livers

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World Journal of Gastroenterology 2004年第9期10卷 1375-1378页

作者： HaoChen Ying-YanYu Ming-JunZhang Xia-XingDeng Wei-PingYang JunJi Cheng-hongPeng hong-weili DepartmentofSurgeryandCenterofOrganTransplantation RuijinHospitalShanghaiSecondMedicalUniversityShanghai200025China CenterofOrganTransplantation RuijinHospitalShanghaiSecondMedicalUniversityShanghai200025China AnimalLaboratory RuijinHospitalShanghaiSecondMedicalUniversityShanghai200025China

AIM: To observe the protective effect of heat shock protein 72 (HSP 72) induced by pretreatment of doxorubicin (DXR) on long-term cold preservation injury of rat ***: Sprague-Dawley rats were administered intravenously DXR at a dose of 1 mg/kg body mass in DXR group and saline in control group. After 48 h, the rat liver was perfused with cold Linger's and University of Wisconsin (UW) solutions and then was preserved in UW solution at 4℃ for 24, 36 and 48 h. AST, ALT, LDH and hyaluronic acid in preservative solution were determined. Routine HE,immunohistochemical staining for HSP 72 and electron microscopic examination of hepatic tissues were ***: After 24, 36 and 48 h, the levels of AST, ALT and hyaluronic acid in preservative solution were significantly higher in control group than in DXR group (P0.05). Hepatic tissues in DXR group were morphologically normal and significantly injured in control group. HSP 72 was expressed in hepatocytes and sinusoidal endothelial cells in DXR group but not in control ***: Pretreatment of DXR may extend the time of rat liver cold preservation and keep liver alive. The expression of HSP 72 in liver can prevent hepatocytes and sinusoidal endothelial cells from long-term cold preservation injury.

关键词：阿霉素肝损伤抗肿瘤药冷冻保存热休克蛋白-72 预处理缺血再灌注

Rapid eradication of antibiotic-resistant bacteria and biofilms by MXene and near-infrared light through photothermal ablation

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Science China Materials 2021年第3期64卷 748-758页

作者： Fan Wu Huiling Zheng Wenzhao Wang Qiong Wu Qi Zhang Jiayu Guo Bangzheng Pu Xinyuan Shi Jiebo Li Xiangmei Chen weili hong Institute of Medical Photonics Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100191China Department of Microbiology and Infectious Disease Center School of Basic Medical SciencesPeking University Health Science CenterBeijing100191China State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of the Chemical PhysicsChinese Academy of SciencesDalian116023China

With the development and rising of antimicrobial resistance,rapid and effective killings of bacteria are urgently needed,especially for antibiotic-resistant bacteria and bacterial biofilms that are usually hard to be treated with conventional ***,a rapid and broad-spectrum antibacterial strategy is demonstrated through photothermal ablation with MXene and ***3C2 MXenes,when combined with 808 nm light,show significant antibacterial effects in just 20 *** antibacterial strategy is effective to 15 bacterial species tested,including methicillin-resistant Staphylococcus aureus(MRSA)and vancomycin-resistant Enterococci(VRE).In addition,the rapid antibacterial strategy works for MRSA biofilms,by damaging the structures as well as killing bacteria in ***,the investigation of the antibacterial mechanisms shows that Ti3C2 with light kills bacteria mainly physically through inserting/contact and photothermal *** work broadens the potential applications of MXene and provides a way to eradicate bacteria and biofilms physically,without the likelihood of resistance development.

关键词： MXenes 2D materials antibiotic-resistance bacteria photothermal

Rapid antimicrobial susceptibility testing for mixed bacterial infection in urine by AI-stimulated Raman scattering metabolic imaging

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Medicine in Novel Technology and Devices 2022年第4期16卷 1-7页

作者： Weifeng Zhang Xun Chen Jing Zhang Xiangmei Chen Liqun Zhou Pu Wang weili hong Instiute of Medical Photonics Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100191China School of Engineering Medicine Beihang UniversityBeijing100191China Department of Urology Peking University First HospitalBeijing100034China Department of Microbiology and Infectious Disease Center School of Basic Medical SciencesPeking University Health Science CenterBeijing100191China

Urinary tract infection with mixed microorganisms may lead to false-positive resistance *** antimicrobial susceptibility testing(AST)performed in clinical laboratories is based on bacterial culture and takes a long time for mixed bacterial ***,we propose a machine learning-based single-cell metabolism inactivation concentration(ML-MIC)model to achieve rapid AST for mixed bacterial *** *** and *** as a demonstration of mixed bacteria,we performed feature extraction and multi-feature analysis on stimulated Raman scattering(SRS)images of bacteria with the ML-MIC model to determine the subtypes and AST of the mixed ***,we assessed the AST of mixed bacteria in urine and obtained single-cell metabolism inactivation concentration in only 3 ***,we demonstrated that SRS imaging of bacterial metabolism can be extended to mixed bacterial infection cases for rapid AST.

关键词： Mixed bacterial infections Antimicrobial susceptibility testing Stimulated Raman scattering Machine learning

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高光谱相干拉曼散射技术及其应用

激光与光电子学进展 2022年第6期59卷 47-66页

作者：吴凡李商羽洪维礼岳蜀华王璞北京航空航天大学生物与医学工程学院北京市生物医学工程高精尖创新中心北京100191 北京大学生物医学前沿创新中心北京100871

生物样品存在着丰富的物质信息,每一种物质都有其自身独特的性质及功能,对这些物质在生物体内的产生、转运、分布等进行研究可以使我们更加深入地了解生物系统。具备良好化学特异性的光学显微镜为这些研究提供了强大且可靠的工具。拉曼... 详细信息

生物样品存在着丰富的物质信息,每一种物质都有其自身独特的性质及功能,对这些物质在生物体内的产生、转运、分布等进行研究可以使我们更加深入地了解生物系统。具备良好化学特异性的光学显微镜为这些研究提供了强大且可靠的工具。拉曼散射技术由于解决了荧光显微镜所需的外源性标记所带来的问题,极大地促进了生物医学的发展。高光谱相干拉曼散射(hyperspectral coherent Raman scattering,HS-CRS)技术可对不同成分物质进行无标记化学成像,通过同时提供图像和光谱信息,对样品内物质的性质、含量和分布状况做出判断,为科研人员提供了更多维度的物质信息。HS-CRS技术在近几十年内进行了不断的技术更新并在生物医学领域完成新的技术应用,本文对主要的HS-CRS技术的实现形式及方案进行了介绍,并总结了HS-CRS技术在生物医学领域的应用。

关键词：高光谱成像相干拉曼散射显微术无标记成像医用光子学

Liver biopsy in evaluation of complications following liver transplantation

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World Journal of Gastroenterology 2004年第11期10卷 1678-1681页

作者： Ying-YanYu JunJi Guang-WenZhou Bai-YongShen HaoChen 3i-QiYan Cheng-hongPeng hong-weili TransplantationCenterandInstituteofDigestiveSurgery RuijinHospitalShanghaiSecondMedicalUniversityShanghai200025China

AIM: To analyze the role of liver biopsies in differential diagnosis after liver transplantation. METHODS: A total of 50 biopsies from 27 patients with liver dysfunction out of 52 liver transplantation cases were included. Biopsies were obtained 0-330 d after operation, in which, 44 were fine needle biopsies, another 6 were wedge biopsies during surgery. All tissues were stained with haemotoxylin-eosin. Histochemical or immunohistochemical stain was done. RESULTS: The rate of acute rejection in detected cases and total transplantation cases was 48.2% and 25.0%, chronic rejection rate in detected cases and total transplantation cases was 14.8% and 7.7%, preservation-repeffusion injury in detected cases and total transplantation cases was 25.9% and 13.5%, hepatic artery thrombosis rate in detected cases and total transplantation cases was 11.1% and 5.8%, intrahepatic biliary injury rate in detected cases and total transplantation cases was 7.4 % and 3.8%, CMV infection rate in detected cases and total transplantation cases was 3.7% and 1.9%, hepatitis B recurrence rate in detected cases and total transplantation cases was 3.7% and 1.9%, the ratio of suspicious drug-induced hepatic injury in detected cases and total transplantation cases was 11.1% and 5.8%. CONCLUSION: Acute rejection and preservation-reperfusion injury are the major factors in early liver dysfunction after liver transplantation. Hepatic artery thrombosis and prolonged cold preservation may result in intrahepatic biliary injury. Acute rejection and viral infection may involve in the pathogenesis of chronic rejection. Since there are no specific lesions in drug-induced hepatic injury, the diagnosis must dosely combine clinical history and rule out other possible complications.

关键词：肝脏活组织检查肝移植并发症手术治疗