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Robotic Intracellular Electrochemical Sensing for Adherent Cells

Cyborg and Bionic Systems 2022年第1期2022卷 75-86页

作者： weikang hu Yanmei Ma Zhen Zhan Danish hussain Chengzhi hu Shenzhen Key Laboratoryof Biomimetic Robotisand Intlligent Systems Department of Mechanicaland Enery EngineeringSouthern University of Science and TechnologyShenzhenChina Department of Mechatronics Engineering National University of Sciences and TechnologyIslamabadPakistan Guangdong Provincial Key Laboratory of human-Augmentation and Rehabilitation Robotics in Universities Southern Universityof Science and TechnologyShenzhenChina

Nanopipette-based observation of intracellular biochemical processes is an important approach to revealing the intrinsic char acteristics and heterogeneity of cells for better investigation of disease progression or early disease ***,the manual operation needs a skilled operator and faces problems such as low throughput and poor *** paper proposes an automated nanopipette-based microoperation system for cell detection,*** nonovershoot positioning of the nanopipette tip in proximity to the cell of interest,cell approaching and proximity detection between nanopipette tip and cell surface,and cell penetration and detection of the intracellular reactive oxygen species(ROS).A robust focus algorithm based on the number of cell contours was proposed for adherent cells,which have sharp peaks while retaining *** automated detection of adherent cells was evaluated on human umbilical cord vein endothelial cells(huVEC)and NIH/3T3 cells,which provided an average of 95.65%true-positive rate(TPR)and 7.59%false-positive rate(FPR)for in-plane cell *** three dimensional nonovershoot tip positioning of the nanopipette was achieved by template matching and evaluated under the interference of *** current feedback was employed for the proximity detection between the nanopipette tip and cell ***,cell penetration and electrochemical detection of ROS were demonstrated on human breast cancer cells and zebrafish embryo *** work provides a systematic approach for automated intracellular sensing for adherent cells,laying a solid foundation for high-throughput detection,diagnosis,and classification of different forms of biochemical reactions within single cells.

关键词： diagnosis proximity retaining

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Tetrathiafulvalene esters with high redox potentials and improved solubilities for non-aqueous redox flow battery applications

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Green Energy & Environment 2024年第5期9卷 899-908页

作者： weikang hu Jiaqi Xu Nanjie Chen Zongcai Deng Yuekun Lai Dongyang Chen College of Materials Science and Engineering Fuzhou UniversityFuzhou350116China College of Chemical Engineering Fuzhou UniversityFuzhou350116China

The exploitation of high performance redox-active substances is critically important for the development of non-aqueous redoxﬂow ***,three tetrathiofulvalene(TTF)derivatives with different substitution groups,namely TTF diethyl ester(TTFDE),TTF tetramethyl ester(TTFTM),and TTF tetraethyl ester(TTFTE),are prepared and their energy storage properties are *** has been found that the redox potential and solubility of these TTF derivatives in conventional carbonate electrolytes increases with the number of ester *** battery with a catholyte of 0.2 mol L^(-1) of TTFTE delivers a speciﬁc capacity of more than 10 Ah L^(-1) at the current density of 0.5 C with two discharge voltage platforms locating at as high as 3.85 and 3.60 V ***/Liþ.Its capacity retention can be improved from 2.34 Ah L^(-1) to 3.60 Ah L^(-1) after 100 cycles by the use of an anion exchange membrane to block the crossover of TTF *** excellent cycling stability of the TIF esters is supported by their well-delocalized electrons,as revealed by the density function theory ***,the introduction of more and larger electron-withdrawing groups is a promising strategy to simultaneously increase the redox-potential and solubility of redox-active ma-terials for non-aqueous redoxﬂow batteries.

关键词： Non-aqueous redox flow batteries Tetrathiofulvarene Redox potential Solubility Substituent effect

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Synergism of preintercalated manganese ions and lattice water in vanadium oxide cathodes for high-capacity and long-life Zn-ion batteries

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Journal of Energy Chemistry 2024年第9期96卷 709-717页

作者： Mengjing Wu Rongrong Li Kai Yang Lijiang Yin weikang hu Xiong Pu Key Laboratory of the Ministry of Education for Advanced Catalysis Material Institute of Physical ChemistryCollege of Chemistry and Materials ScienceZhejiang Normal UniversityJinhua 321004ZhejiangChina CAS Center for Excellence in Nanoscience Beijing Key Laboratory of Micro-nano Energy and SensorBeijing Institute of Nanoenergy and NanosystemsChinese Academy of SciencesBeijing 101400China School of Nanoscience and Engineering University of Chinese Academy of SciencesBeijing 100049China Chilwee Power Co.Ltd. Changxing 313100ZhejiangChina

Aqueous Zn-ion batteries(AZIBs)are recognized as a promising energy storage system with intrinsic safety and low cost,but its applications still rely on the design of high-capacity and stable-cycling cathode *** this work,we present an intercalation mechanism-based cathode materials for AZIB,*** vanadium oxide with pre-intercalated manganese ions and lattice water(noted as MVOH).The synergistic effect between Mn^(2+)and lattice H_(2)O not only expands the interlayer spacing,but also significantly enhances the structural *** in-situ and ex-situ characterizations clarify the Zn^(2+)/H^(+)co–(de)intercalation mechanism of MVOH in aqueous *** demonstrated remarkable structure stability,excellent kinetic behaviors and ion-storage mechanism together enable the MVOH to demonstrate satisfactory specific capacity of 450 mA h g^(−1)at 0.2 A g^(−1),excellent rate performance of 288.8 mA h g^(−1)at 10 A g^(−1)and long cycle life over 20,000 cycles at 5 A g^(−1).This work provides a practical cathode material,and contributes to the understanding of the ion-intercalation mechanism and structural evolution of the vanadium-based cathode for AZIBs.

关键词： Zn-ion batteries Vanadium oxide Pre-intercalation Lattice water Manganese ion

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Antibacterial Electrospun Nanofibrous Materials for Wound Healing

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Advanced Fiber Materials 2023年第1期5卷 107-129页

作者： Zijian Wang weikang hu Wang Wang Yu Xiao Yun Chen Xinghuan Wang Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Biomedical Engineering and hubei Province Key Laboratory of Allergy and Immune Related Disease TaiKang Medical School(School of Basic Medicine Sciences)Wuhan UniversityWuhan 430071China Ministry of Education Key Laboratory of the Green Preparation and Application for Functional Materials Hubei Key Laboratory of Polymer MaterialsSchool of Materials Science and EngineeringHubei UniversityWuhan 430062China

One of the leading causes of wound healing delays is bacterial infection,which limits the process of restoring the histological and functional integrity of the *** nanofibrous materials(ENMs)are biocompatible and biodegradable,and they can provide specific physical,chemical,and biological cues to accelerate wound *** on this fact,a series of multifunctional ENMs for complex clinical applications,particularly infected skin injuries,have been ***-biotics,antimicrobial peptides(AMPs),metals and metal oxides(MMOs),and antibacterial polymers have previously been incorporated into ENMs through advanced material processing techniques,endowing ENMs with enhanced and excellent antibacterial *** review summarizes wound healing issues and provides recent advances in antibacterial ENMs created by cutting-edge *** future of clinical and translational research on ENMs is also discussed.

关键词： Electrospinning Nanofiber Wound dressing Antibacterial activity Skin tissue engineering

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Defect electrons accelerate iron cycle of novel Fe-based Fenton:Long-term effective quinoline degradation

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Chinese Chemical Letters 2023年第6期34卷 598-605页

作者： weikang hu Ming Yang Qingyun Yan Jiahui Ji Yan Bao Jinlong Zhang Mingyang Xing Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Feringa Nobel Prize Scientist Joint Research CenterFrontiers Science Center for Materiobiology and Dynamic ChemistryInstitute of Fine ChemicalsSchool of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghai 200237China Shanghai Engineering Research Center for Multi-media Environmental Catalysis and Resource Utilization East China University of Science and TechnologyShanghai 200237China

Heterogeneous Fenton has been widely used in the disposal of organic pollutants,however,slow regeneration of≡Fe(II)remains limitation for its practical application of long-term ***,we come up with a novel Fe-based heterogeneous Fenton catalyst named as FeS_(x)O_(y)-X(X is the ratio of ethylene glycol to N,N-dimethylformamide).With the help of the abundant defect electrons in Sulfur vacancies,≡Fe(Ⅱ)regeneration on the surface of FeS_(x)O_(y)-1:1 was accelerated,resulting in a stable proportion of≡Fe(Ⅱ)on the surface,which maintained continuously stable generation of hydroxyl radical(·OH)and singlet oxygen(^(1)O_(2)).Thus,without any organic reagents or cocatalysts,FeS_(x)O_(y)-1:1 based Fenton system achieved effective long-term degradation of 560 mg/L quinoline within only 7 days,which was evidently better than reported Fe S and SV-Fe S_(2)(SV:Sulfur vacancy).The system had excellent adaptability to water quality and the COD removal rate of biochemical wastewater was as high as 79.8%.

关键词： Fenton reaction Quinoline Defect electron Iron cycle Heterogeneous catalyst

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Facile fabrication of quaternized chitosan-incorporated biomolecular patches for non-compressive haemostasis and wound healing

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Fundamental Research 2024年第5期4卷 1243-1253页

作者： Zesheng Chen Yixuan Zhang Kexin Feng Tao hu Bohan huang Jinlan Tang Junjie Ai Liang Guo weikang hu Zijian Wang Department of Urology Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research CenterZhongnan Hospital of Wuhan UniversityWuhan 430071China Ministry of Education Key Laboratory of the Green Preparation and Application for Functional Materials Hubei Key Laboratory of Polymer MaterialsSchool of Materials Science and EngineeringHubei UniversityWuhan 430062China Department of Plastic Surgery Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Clinical Laboratory Hubei Provincial Hospital of Traditional Chinese MedicineWuhan 430061China

Cell-free wound dressings (WDs) with desirable effectiveness and safety have received much attention in the field of regenerative medicine. However, the weak linkages between bioactive polymers and the spatial structure of WDs frequently result in interventional treatment failure. Herein, we create a series of quaternized chitosan (QCS)-incorporated composite hydrogels (referred to as GHCH-n) by UV cross-linking and then convert them into microneedle patches (MNPs). QCS, which is positively charged and amphiphilic, is essential for broad-spectrum antibacterial and haemostatic activities. QCS is proven to be slightly toxic, so it is immobilized into the methacrylate gelatine (GelMA) molecular cage to minimize adverse effects. A polydimethylsiloxane micro-mould is used to shape the MNPs. MNPs can pierce tissue, seal off bleeding sites, and cling to wounds securely. Thus, MNPs can cooperate with GHCH-n hydrogels to halt bleeding and accelerate wound healing. This study recommends GHCH-10 MNPs as an advanced biomaterial. Several preclinical research models have thoroughly validated the application effect of GHCH-10 MNPs. This research also proposes a novel strategy for integrating the nature of bioactive polymers and the structure of composite biomaterials. This strategy is not only applicable to the fabrication of next-generation WDs but also shows great potential in expanding interdisciplinary domains.

关键词： Quaternized chitosan Patches Biocompatible Antibacterial Haemostatic Wound healing

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ELECTROCHEMICAL　PROPERTIES　OF　THE　HYDROGEN　ABSORPTION　OF　AMORPHOUS　Ml－Ni　ALLOY　FILMS

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Acta Metallurgica Sinica(English Letters) 1995年第2期8卷 123-125页

作者： hu weikang ZHANG Yunshi SONG Deying LUO Daojun WANG Yun(Institute of New Energy Material Chemistry.Nankai University.Tianjin.China) Inst of New Energy Material Chemistry Nankai Univ Tianjin China

The amorphous Ml-Ni films were prepared by means of ion beam sputtering the electrochemical hydrogen storage,discharge ability and durability of amorphous film electrodes are investigated,The results show that the maximum capacities of the amorphous MlNi_(1.79),MlNi_(2.52),and MlNi_(3.44) samples are 90,100 and 142 mAh/g,respectively,and the MlNi_(3.44) amorphous film does not off and disintegrate into fine particles a fier more than 580 cycles.

关键词： amorphous film.Ml-Ni alloy electrochemical hydrogen absorption

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DL-GAN生成对抗网络的半监督语义分割模型

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计算机工程与应用 2024年第19期60卷 221-229页

作者：刘凡段先华胡维康江苏科技大学计算机学院江苏镇江212100

语义分割目前主流的全监督学习方式、数据质量和数量决定了网络的训练效果。只有花费大量的标注成本,才能得到质量高且数据量大的训练数据。根据上述情况,出现了基于半监督学习方式的语义分割。半监督学习可以节省数据的标注成本,很好... 详细信息

语义分割目前主流的全监督学习方式、数据质量和数量决定了网络的训练效果。只有花费大量的标注成本,才能得到质量高且数据量大的训练数据。根据上述情况,出现了基于半监督学习方式的语义分割。半监督学习可以节省数据的标注成本,很好地解决需要大量标注成本的问题,越来越多人开始关注半监督学习的图像语义分割。根据图像语义分割方法目前的发展现状,提出了一种结合DeepLabv2的生成对抗网络(DL-GAN)的半监督语义分割的模型。将DeepLabv2作为生成对抗网络的生成网络,完全卷积的网络作为判别器网络;对生成网络进行改进,首次将CBAM注意力机制和深度可分离卷积结合应用于DeepLabv2,将其作为生成网络,具体一是在DeepLabv2最后的卷积层前添加CBAM注意力机制,二是将DeepLabv2网络中Resnet残差块的标准卷积替换为深度可分离卷积,使整个模型将权重参数更为合理地分配,提高模型的表征能力且计算更为高效,加快训练效率;用空洞卷积替换判别器的标准卷积,提升整个判别器的感受野,提高训练效果,提升语义分割精度。该方法在PASCAL VOC 2012数据集上的实验结果相对于Affinitynet网络平均交并比提高6.3个百分点,证明了提出方法是有效的。

关键词：生成对抗网络注意力机制语义分割深度可分离卷积

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改进的最大最小特征值之差的频谱感知算法

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电子技术应用 2014年第8期40卷 119-121,125页

作者：赵知劲胡伟康杭州电子科技大学通信工程学院浙江杭州310018

针对传统的最大最小特征值之差的频谱感知算法(DMM),从提高特征值估计精度出发,引入了信号矩阵拆分重组的过程,提出了一种改进的协作频谱感知算法(IDMM)。该算法在逻辑上增加了协作用户数,降低了协作用户数少对频谱感知性能造成的影响... 详细信息

针对传统的最大最小特征值之差的频谱感知算法(DMM),从提高特征值估计精度出发,引入了信号矩阵拆分重组的过程,提出了一种改进的协作频谱感知算法(IDMM)。该算法在逻辑上增加了协作用户数,降低了协作用户数少对频谱感知性能造成的影响。理论分析和仿真结果均表明,IDMM算法性能明显优于DMM算法。

关键词：频谱感知随机矩阵理论最大最小特征值之差拆分重组

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基于贝叶斯预测密度的弱匹配追踪频谱检测

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计算机应用研究 2015年第7期32卷 2119-2122页

作者：赵知劲胡伟康杭州电子科技大学通信工程学院杭州310018 中国电子科技集团第36研究所通信系统信息控制技术国家级重点实验室浙江嘉兴314001

针对在低信噪比、观测点数较少情况下稀疏度的欠估计问题,提出了一种基于贝叶斯预测密度的弱匹配追踪频谱检测算法。该算法利用贝叶斯预测密度理论推导出罚函数,然后引入弱匹配策略于Co Sa MP算法,提高频谱支撑集估计性能,且减弱受稀疏... 详细信息

针对在低信噪比、观测点数较少情况下稀疏度的欠估计问题,提出了一种基于贝叶斯预测密度的弱匹配追踪频谱检测算法。该算法利用贝叶斯预测密度理论推导出罚函数,然后引入弱匹配策略于Co Sa MP算法,提高频谱支撑集估计性能,且减弱受稀疏度估计准确度的影响。仿真结果表明,当信噪比高于3 d B时,利用400个观测样本该算法就能获得90%以上的频谱检测概率,宽带频谱感知性能优于已有算法。

关键词：宽带频谱感知贝叶斯预测密度稀疏度弱匹配追踪

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