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Laboratory Simulation of Vertical Hydrocarbon Microseepage

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Acta Geologica Sinica(English Edition) 2011年第1期85卷 223-232页

作者： WANG Guojian chenG Tongjin FAN Ming REN Chun chen weijun Wuxi Research Institute of Petroleum Geology Exploration and Production Research Institute SINOPEC Wuxi 214151 Jiangsu China

Based on a conceptual model of hydrocarbon microseepage, a macro-sized experimental equipment used the matched mixtures of cement and quartz sand as simulated caprock and its overlying strata is first set up to simulate the processes of hydrocarbon microseepage and its nearsurface expressions. The results of the simulation experiments suggest that simulated caprock and its overlying strata have a certain sealed capability; hydrocarbon microseepage is dominated by the pressure of point gas source, and temperature plays only a subordinate role; on the path of hydrocarbon microseepage, the distribution of hydrocarbon concentration is fan-shaped; differential adsorption of aikanes by the simulated caprock and its overlying strata results in the occurrence of a chromatographic effect. Different migrating patterns within simulated caprock are shown by the ratio of i-butane to n-butane. The concentration of hydrocarbon in the surface soil has good correspondence with the pressure of point gas source. These simulation results are significant to further study of the mechanism of anomalies recovered in surface geochemistry exploration.

关键词： hydrocarbon microseepage simulation experiment equipment surface geochemistryexploration

Growth performance,fatty acid composition,and lipid metabolism are altered in groupers(Epinephelus coioides)by dietary fish oil replacement with palm oil

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Animal Nutrition 2022年第1期8卷 102-113页

作者： Yingmei Qin Lingyun He Yanfei Wang Dong Li weijun chen Jidan Ye Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation Fisheries College of Jimei UniversityXiamen 361021China

In this study,we conducted a 56-d feeding trial to investigate the effects of replacing the fish oil(FO)with palm oil(PO)on the performance,tissue fatty acid(FA)composition,and mRNA levels of genes related to hepatic lipid metabolism in grouper(Epinephelus coioides).Five isolipidic(13%crude lipid)and isonitrogenous(48%CP)diets were formulated by incrementally adding PO to the control diet(25%fish meal and 9%added FO)to replace FO in the control *** groups of 30 groupers(initial weight:12.6±0.1 g)were fed one of the diets twice daily,to apparent *** replacement of FO with 50%PO revealed maximum growth without affecting the performance and whole-body proximate compositions,and replacing FO with 100%PO revealed a comparable(P>0.05)growth with that of the control diet,suggesting PO as a suitable alternative to *** analysis of FA profiles in the dorsal muscle and liver though reflected the FA profile of the diet,PO substitutions above 50%could compromise(P<0.05)the FA profile in the liver and flesh of the fish species in comparison with the control ***,the mRNA levels of FAS,G6PD,LPL,PPARА,and D6FAD genes in the liver had positive linear and/or quadratic responses,but the SCD,HSL,ATGL,FABP,SREBP-1C and ELOVL5 had the opposite trend,with increasing dietary PO inclusion levels,whereas the mRNA level of ACC was not affected by dietary *** optimal level of PO substitution for FO was estimated to be 47.1%of the feed,based on the regression analysis of percent weight gains against dietary PO inclusion levels;however,it might affect the FA profile in the liver and flesh of the fish species,and further study is required to investigate whether the changes in tissue FA composition will affect the welfare and market value over a production cycle of grouper.

关键词： Dietary lipid Epinephelus coioides Fatty acid metabolism Growth performance

cDNA cloning and expression of erythropoietin in the plateau zokor(Myospalax baileyi) from the Qinghai-Tibet Plateau

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Chinese Science Bulletin 2012年第9期57卷 997-1006页

作者： WANG ZhenLong chen Yan YANG Jie chen weijun ZHANG YanMing ZHAO XinQuan Department of Bioengineering Zhengzhou University Zhengzhou 450001 China Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Plateau Institute of Biology Chinese Academy of Sciences Xining 810001 China Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100029 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China Department of Epidemiology and Statistics School of Public Health Hebei Medical University Shijiazhuang 050017 China

All organisms respond to variation in their environments and manage environmental stress through metabolic *** plateau zokor(Myospalax baileyi) is an endemic and keystone subterranean rodent species that inhabits the Qinghai-Tibet Plateau between 2800 and 4200 m above sea *** is a hypoxic-tolerant mammal with a high ratio of oxygen utilization that enables it to cope with its harsh *** explore the molecular mechanism of altitude acclimatization of the plateau zokor,we cloned the zokor erythropoietin(Epo) gene and used real-time PCR to compare Epo mRNA levels in zokors inhabiting 16 different *** full-length zokor Epo open reading frame was 579 bp that encoded a precursor peptide of 192 amino acids with a signal peptide of 26 *** Epo gene of the plateau zokor was 81%-95% homologous to that of human,mouse,rat,root vole and the Golan Heights blind mole rat,with the highest homology(95%) to species of the genus *** mRNA was detected mainly in the zokor kidney and spleen among 8 selected *** level of Epo mRNA increased in the liver and kidney with increases in *** increase in the kidney was 5 times that in the ***,expression of Epo mRNA in the kidney of zokors living at the highest altitude(4268 m) was 12-fold higher than that of zokors living at the lowest(2492 m) *** findings provide essential information for understanding the possible role of Epo in adaptation to hypoxia in the plateau zokor.

关键词：促红细胞生成素高原鼢鼠克隆和表达青藏高原 cDNA mRNA水平环境压力基因检测

Petroleum Distribution Characteristics of the Americas and the Exploration Prospect Analysis

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Acta Geologica Sinica(English Edition) 2018年第1期92卷 378-393页

作者： HAO Qingqing LI Lintao ZUO Yinhui chen weijun WU Lei YI Junjie Institute of Mineral Resources Research China Metallurgical Geology Bureau Beijing 101300 China CNOOC Research Institute Beijing 100028 China College of Energy Resources Chengdu University of Technology Chengdu 610059 Sichuan China

The world＇s present demand for oil and gas is still in a rapid growth period, and traditional oil and gas resources account for more than 60% of the global oil and gas supply. The Americas is the world＇s second largest production and consumption center of liquid fuel, and is also the world＇s largest natural gas producer. In 2016, the Americas had 85.3 billion tons of proven oil reserves and 18.7 trillion m3 of proven natural gas reserves, which account for 35.4% and 10.0% of world＇s total reserves, respectively. It produced 1267.1 Mt of oil and 1125.4 billion m3 of natural gas, which account for 28.9% and 31.7% of the world＇s total production, respectively. The crude oil and natural gas reserves are mainly distributed in the U.S., Canada and Venezuela. The U.S. is the earliest and most successful country in shale gas exploration and development, and its shale gas is concentrated in the southern, central and eastern U.S., including the Marcellcus shale, Barnett shale, EagleFord shale, Bakken shale, Fayettevis shale, Haynsvill shale, Woodford shale and Monterey/Santos shale. The potential oil and gas resources in the Americas are mainly concentrated in the anticline and stratigraphic traps in the Middle- Upper Jurassic slope deposition of the North Slope Basin, the Paleozoic Madsion group dolomite and limestone in the Williston Basin, dominant stratigraphic traps and few structural traps in the Western Canada Sedimentary Basin, the Eocene structural-stratigraphic hydrocarbon combination, structural- unconformity traps and structural hydrocarbon combination, and the Upper Miocene stratigraphic- structural hydrocarbon combination in the Maracaibo Basin of Venezuela, the stratigraphic-structural traps and fault horst, tilting faulted blocks and anticlines related to subsalt structure and basement activity in the Campos Basin, the subsalt central low-uplift belt and supra-salt central low-uplift belt in the Santos Basin of Brazil, and the structural-stratigraphic traps in

关键词： Americas oil and gas supply and demand U.S. Canada Venezuela unconventional oil andgas resources

The Distribution of Petroleum Resources and Characteristics of Main Petroliferous Basins along the Silk Road Economic Belt and the 21st-Century Maritime Silk Road

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Acta Geologica Sinica(English Edition) 2017年第4期91卷 1457-1486页

作者： HAO Qingqing ZUO Yinhui LI Lintao chen weijun YI Junjie WU Lei Institute of Mineral Resources Research China Metallurgical Geology Bureau Beijing 101300 China College of Energy Resources Chengdu University of Technology Chengdu 610059 Sichuan China CNOOC Research Institute Beijing 100028 China

The Silk Road Economic Belt and the 21st-Century Maritime Silk Road Initiative, abbreviated as the Belt and Road Initiative, is a primary development strategy of China＇s future international cooperation. Especially, the energy resource cooperation, including oil and gas resources cooperation, is an important part of this initiative. The Belt and Road has undergone complicated geological evolution, and contains abundant mineral resources such as oil, gas, coal, uranium, iron, copper, gold and manganese ore resources. Among these, Africa holds 7.8% of the world＇s total proven oil reserves. The oil and gas resources in Africa are relatively concentrated, with an overall low exploration degree and small consumption demand. Nigeria and Libya contain the most abundant oil resources in Africa, accounting for 2.2% and 2.9% of the world＇s total reserves, respectively. Nigeria and Algeria hold the richest natural gas resources in Africa, occupying 2.8% and 2.4% of the world＇s total reserves, respectively. Africa＇s oil and gas resources are mainly concentrated in Egypt, Sultan and Western Sahara regions in the northern Africa, and the Gulf of Guinea, Niger River and Congo River area in the western Africa. The Russia--Central Asia area holds rich petroleum resources in Russia, Kazakhstan, Turkmenistan and Uzbekistan. The potential oil and gas areas include the West Siberia Basin, East Siberia Basin and sea continental shelf in Russia, the northern and central Caspian Basin in Kazakhstan, the right bank of the Amu-Darya Basin, the East Karakum uplift and the South Caspian Basin in Turkmenistan, and the Amu-Daria Basin, Fergana Basin, Afghan-Tajik Basin and North Ustyurt Basin in Uzbekistan. The Middle East oil and gas resources are mainly distributed in the Zagros foreland basin and Arabian continental margin basin, and the main oil-producing countries include Saudi Arabia, Iran and Iraq. The Asia Pacific region is a new oil and gas consumption center, with rapid growth of oil a

关键词： petroliferous basin pay zone Africa Middle East Central Asia Russia Asia Pacific region

Generation of breathing solitons in the propagation and interactions of Airy–Gaussian beams in a cubic–quintic nonlinear medium

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Chinese Physics B 2018年第11期27卷 528-533页

作者： weijun chen Ying Ju Chunyang Liu Liankai Wang Keqing Lu School of Science Changchun University of Science and Technology Changchun 130022 China Institute of Electronics and Information Engineering Tianjin Polytechnic University Tianjin 300387 China

Using the split-step Fourier transform method, we numerically investigate the generation of breathing solitons in the propagation and interactions of Airy–Gaussian（AiG） beams in a cubic–quintic nonlinear medium in one transverse dimension. We show that the propagation of single AiG beams can generate stable breathing solitons that do not accelerate within a certain initial power range. The propagation direction of these breathing solitons can be controlled by introducing a launch angle to the incident AiG beams. When two AiG beams accelerated in opposite directions interact with each other,different breathing solitons and soliton pairs are observed by adjusting the phase shift, the beam interval, the amplitudes,and the light field distribution of the initial AiG beams.

关键词： cubic-quintic nonlinear medium Airy-Gaussian beams propagation and interactions breathing solitons

A high-performance electrochromic device assembled with WO_(3)/Ag and TiO_(2)/NiO composite electrodes towards smart window

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Frontiers of Materials Science 2024年第1期18卷 123-135页

作者： Haorui Liu Jikai Yang Decai Nie Chunlei Liu Liumenghan Zheng Yining Mu weijun chen Chongqing Research Institute Changchun University of Science and TechnologyChongqing 400000China School of Physics Changchun University of Science and TechnologyChangchun 130022China

The choice of cathode and anode materials for electrochromic devices plays a key role in the performance of electrochromic smart *** this research,WO_(3)/Ag and TiO_(2)/NiO composite thin films were separately prepared by the hydrothermal method combined with *** electrochromic properties of the single WO_(3) thin film were optimized,and TiO_(2)/NiO composite films showed better electrochromic performance than that of the single NiO ***_(3)/Ag and TiO_(2)/NiO composite films with excellent electrochromic properties were respectively chosen as the cathode and the anode to construct a WO_(3)/Ag‒TiO_(2)/NiO electrochromic *** response time(tc=4.08 s,tb=1.08 s),optical modulation range(35.91%),and coloration efficiency(30.37 cm^(2)·C^(-1))of this electrochromic device are better than those of WO_(3)-NiO and WO_(3)/Ag-NiO electrochromic *** work provides a novel research idea for the performance enhancement of electrochromic smart windows.

关键词： WO_(3)/Ag composite film TiO_(2)/NiO composite film electrochromic device

A molecular link between autophagy and circadian rhythm in plants

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Journal of Integrative Plant Biology 2022年第5期64卷 1044-1058页

作者： weijun chen Zhaotun Hu MengTing Yu Sirui Zhu Junjie Xing Limei Song Wenxuan Pu Feng Yu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biologyand Hunan Province Key Laboratory of Plant Functional Genomics and Developmental RegulationHunan UniversityChangsha 410082China Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province School of Biological and Food EngineeringHuaihua CollegeHuaihua 418008China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125China Technology Center China Tobacco Hunan Industrial Co.Ltd.Changsha 410007China

Extremely high or low autophagy levels disrupt plant survival under nutrient ***,autophagy has been reported to display rhythms in ***,the mechanism of circadian regulation of autophagy is still ***,we observed that autophagy has a robust rhythm and that various autophagy-related genes(ATGs)are rhythmically expressed in *** immunoprecipitation(Ch IP)and dual-luciferase(LUC)analyses showed that the core oscillator gene TIMING OF CAB EXPRESSION 1(TOC1)directly binds to the promoters of ATG(ATG1 a,ATG2,and ATG8 d)and negatively regulates autophagy activities under nutritional ***,autophagy defects might affect endogenous rhythms by reducing the rhythm amplitude of TOC1 and shortening the rhythm period of CIRCADIAN CLOCK-ASSOCIATED 1(CCA1).Autophagy is essential for the circadian clock pattern in seedling development and plant sensitivity to nutritional *** together,our studies reveal a plant strategy in which the TOC1-ATG axis involved in autophagy-rhythm crosstalk to fine-tune the intensity of autophagy.

关键词： ATG8d autophagy activities circadian clock nutritional stress TOC1

Study of copper-cysteamine based X-ray induced photodynamic therapy and its effects on cancer cell proliferation and migration in a clinical mimic setting

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Bioactive Materials 2022年第1期7卷 504-514页

作者： Xiangyu chen Jiayi Liu Ya Li Nil Kanatha Pandey Taili chen Lingyun Wang Eric Horacio Amador weijun chen Feiyue Liu Enhua Xiao Wei chen Department of Radiology The Second Xiangya Hospital of Central South UniversityChangshaHunanChina Department of Physics The University of Texas at ArlingtonArlingtonTX76019USA Department of Oncology Xiangya HospitalCentral South UniversityChangshaHunan Province410011China Department of Oncology The Second Xiangya Hospital of Central South UniversityChangshaHunanChina

Copper-cysteamine as a new generation of sensitizers can be activated by light,X-rays,microwaves,or ultrasound to produce reactive oxygen species.X-ray induced photodynamic therapy(X-PDT)has been studied extensively;however,most of the studies reported so far were conducted in the laboratory,which is not conducive to the clinical translation *** this contribution,for the first time,we investigated the treatment efficiency of copper-cysteamine(Cu-Cy)based X-PDT by mimicking the clinical conditions with a clinical linear accelerator and building deep-seated tumor models to study not only the effectiveness but also its effects on the cell migration and proliferation in the level of the cell,tissue,and *** results showed that,without X-ray irradiation,Cu-Cy nanoparticles(NPs)had a low toxicity in HepG2,SK-HEP-1,Li-7,and 4T1 cells at a concentration below 100 mg/***,for the first time,it was observed that Cu-Cy mediated X-PDT can inhibit the proliferation and migration of these cell lines in a dose-dependent *** markers of migration and cell proliferation,proliferating cell nuclear antigen(PCNA)and E-cadherin,from tumor tissue in the X-PDT group were remarkably different from that of the control ***,the MRI assessment showed that the Cu-Cy based X-PDT inhibited the growth of deeply located tumors in mice and rabbits(p<0.05)without any obvious toxicities in ***,these new findings demonstrate that Cu-Cy NPs have a safe and promising clinical application prospect in X-PDT to improve the efficiency of radiotherapy(RT)for deep-seated tumors and effectively inhibit tumor cell proliferation and migration.

关键词： Copper-cysteamine Nanoparticle Photodynamic therapy X-ray Cell migration Proliferation