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The H^(+)-pyrophosphatase IbVP1 regulates carbon flux to influence the starch metabolism and yield of sweet potato

Horticulture Research 2021年第1期8卷 343-354页

作者： weijuan fan Yandi Zhang Yinliang Wu Wenzhi Zhou Jun Yang Ling Yuan Peng Zhang Hongxia Wang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China Department of Plant and Soil Sciences and Kentucky Tobacco Research and Development Center University of KentuckyLexingtonKY 40546USA

Storage roots of sweet potato are important sink organs for photoassimilates and energy,and carbohydrate metabolism in storage roots affects yield and starch *** previous study showed that sweet potato H+-pyrophosphatase(IbVP1)plays a vital role in mitigating iron deficiency and positively controls fibrous root ***,its roles in regulating starch production in storage roots have not been *** this study,we found that IbVP1 overexpression in sweet potato improved the photosynthesis ability of and sucrose content in source leaves and increased both the starch content in and total yield of sink *** 13C-labeled sucrose feeding,we determined that IbVP1 overexpression promotes phloem loading and sucrose long-distance transport and enhances Pi-use *** sweet potato plants overexpressing IbVP1,the expression levels of starch biosynthesis pathway genes,especially AGPase and GBSSI,were upregulated,leading to changes in the structure,composition,and physicochemical properties of stored *** study shows that the IbVP1 gene plays an important role in regulating starch metabolism in sweet *** of the VP1 gene in genetic engineering of sweet potato cultivars may allow the improvement of starch production and yield under stress or nutrient-limited conditions.

关键词： cultivar metabolism starch

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Engineering geological conditions and features of geologic hazards in Wudongde reservoir of Jinshajiang River valley

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Global Geology 2010年第3期13卷 145-150页

作者： LIU weijuan fan Jianhua ZHANG Yange College of Construction Engineering Jilin University Changchun 130026 China

There is a great difference between the distribution and evolvement characteristics of slope geological hazard in the same geographical location and climatic conditions,taking the similar structural-genetic connection in Wudongde reservoir area of Jinshajiang River valley for example. In all engineering geological conditions,the chronologic age and attitude of strata,and the lithologic association factors control the distributions and evolvement characteristics of slope geological hazard in the studied area. The study shows that the slopes in geological evolution are in different stages. The conclusion helps to understand the types and the intensity of geological disasters.

关键词： Jinshajiang River Wudongde reservoir engineering geological condition slope geological hazard distribution and evolvement characteristics

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Development of Transgenic Sweet Potato(Ipomoea batatas) Plants Tolerant to Salt Stress

Development of Transgenic Sweet Potato(Ipomoea batatas) Plan...

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2010全国植物生物学研讨会

作者： weijuan fan Peng Zhang Shanghai Center for Cassava Biotechnology Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

Sweet potato is a herbaceous perennial root crop and provides major feedstock for food and biofuel industries in *** stresses,such as salinity,drought and low temperature,are critical delimiters for high productivity ... 详细信息

关键词： sweet potato over-expression Na+/H+ antiporter betaine aldehyde dehydrogenase

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Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq

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Molecular Plant 2021年第4期14卷 556-570页

作者： Mengjiao Chen weijuan fan Feiyang Ji Hua Hua Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong fan Qin Wang Shufeng Zhang Guiling Liu Zhe Sun Changgeng Tian Fengling Zhao Jianli Zheng Qi Zhang Jiaxin Chen Jie Qiu Xin Wei Ziru Chen Peng Zhang Dong Pei Jun Yang Xuehui Huang Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai Chenshan Botanical GardenShanghai 201602China State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Tai'an Academy of Agricultural Sciences Tai'an 271000ShandongChina National Genomics Data Center Bio-Med Big Data CenterCAS Key Laboratory of Computational BiologyCAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China CAS Center for Excellence of Molecular Plant Sciences Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200233China These authors contributed equally to this article.

Many important crops(e.g.,tuber,root,and tree crops)are *** these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid *** this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing *** applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic *** causative genes at these loci were detected based on functional *** together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient *** OutcrossSeq software and its instruction manual are available for downloading at ***/tool/***.

关键词： genotyping genetic mapping outcrossing polyploid

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Engineering geological conditions and features of geologic hazards in Wudongde reservoir of Jinshajiang River valley

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Global Geology 2010年第Z1期 145-150页

作者： LIU weijuan,fan Jianhua and ZHANG Yange College of Construction Engineering,Jilin University,Changchun 130026,China

关键词： Jinshajiang River Wudongde reservoir engineering geological condition slope geological hazard distribution and evolvement characteristics

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Haplotype-based phylogenetic analysis and population genomics uncover the origin and domestication of sweetpotato

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Molecular Plant 2024年第2期17卷 277-296页

作者： Mengxiao Yan Ming Li Yunze Wang Xinyi Wang M-Hossein Moeinzadeh Dora G.Quispe-Huamanquispe weijuan fan Yijie fang Yuqin Wang Haozhen Nie Zhangying Wang Aiko Tanaka Bettina Heider Jan F.Kreuze Godelieve Gheysen Hongxia Wang Martin Vingron Ralph Bock Jun Yang Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai 201602China College of Life Sciences Chongqing Normal UniversityChongqing 401331China Biotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural SciencesChengdu 610061China College of Life Sciences Shanghai Normal UniversityShanghai 200234China Department of Computational Molecular Biology Max Planck Institute for Molecular GeneticsIhnestraBe 63-7314195 BerlinGermany Department of Biotechnology Ghent University9000 GhentBelgium Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Crop Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China Graduate School of Bioagricultural Sciences Nagoya UniversityChikusaNagoya 464-8601Japan international Potato Center(CIP) LimaPeru CAS Center for Excellence of Molecular Plant Sciences Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200233China Max-Planck-Institut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany

The hexaploid sweetpotato(lpomoea batatas)is one of the most important root crops ***,its genetic origin remains controversial,and its domestication history remains *** this study,we used a range of genetic evidence and a newly developed haplotype-based phylogenetic analysis to identify two probable progenitors of *** diploid progenitor was likely closely related to lpomoea ae-quatoriensis and contributed the B,subgenome,IbT-DNA2,and the lineage 1 type of chloroplast genome to *** tetraploid progenitor of sweetpotato was most likely *** 4x,which donated the B2 subgenome,IbT-DNA1,and the lineage 2 type of chloroplast *** most likely originated from reciprocal crosses between the diploid and tetraploid progenitors,followed by a subsequent whole-genome *** addition,we detected biased gene exchanges between the subgenomes;the rate of B,to B2 subgenome conversions was nearly three times higher than that of B2 to B subgenome *** analyses revealed that genes involved in storage root formation,maintenance of genome stabil-ity,biotic resistance,sugar transport,and potassium uptake were selected during the speciation and domestication of *** study sheds light on the evolution of sweetpotato and paves the way forimprovementofthiscrop.

关键词： sweetpotato origin gene conversion domestication IbT-DNA HPA

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基于大数据的医院抢救车内急救药品应用分析

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护理学杂志 2019年第14期34卷 36-39页

作者：石莹范伟娟何英赵丽萍中南大学湘雅护理学院湖南长沙410006 中南大学湘雅二医院党委宣传部中南大学湘雅二医院康复科

目的分析综合医院抢救车内急救药品使用情况,为进一步规范抢救车内急救药品配置提供参考。方法通过信息管理中心收集1所三级甲等综合医院2016~2018年35个专科抢救用药信息,基于年消耗量、用药频度及专科使用率进行统计分析。结果3年内,... 详细信息

目的分析综合医院抢救车内急救药品使用情况,为进一步规范抢救车内急救药品配置提供参考。方法通过信息管理中心收集1所三级甲等综合医院2016~2018年35个专科抢救用药信息,基于年消耗量、用药频度及专科使用率进行统计分析。结果3年内,肾上腺素、去甲肾上腺素的年消耗量、用药频度及专科使用率均较高;异丙肾上腺素、氨茶碱、异丙嗪均较低。急救药品清单中50%的药品专科使用率低于60%。各药品的年消耗量、用药频度及专科使用率间均存在相关性(均P<0.05)。结论当前抢救车内急救药品的基数与种类有待调整,且需考虑专科差异。

关键词：抢救车急救药品限定日剂量用药信息年消耗量用药频度专科使用率

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Development of Transgenic Sweet Potato(Ipomoea batatas) Plants Tolerant to Salt Stress

Development of Transgenic Sweet Potato(Ipomoea batatas) Plan...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： weijuan fan and Peng Zhang~* Shanghai Center for Cassava Biotechnology,Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,300 Fenglin Road,Shanghai 200032,China

关键词： sweet potato over-expression Na+/H+ antiporter betaine aldehyde dehydrogenase

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Effects of carbon content on the microstructure and tensile properties of a low-density steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 391-401页

作者： Yongxuan Shang Mingyu fan Shuyong Jiang Zhongwu Zhang Key Laboratory of Superlight Materials and Surface Technology Ministry of EducationCollege of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001China State Key Laboratory of Metal Material for Marine Equipment and Application Iron&Steel Research Institute of Ansteel Group CorporationAnshan 114009China

Carbon can change the phase components of low-density steels and influence the mechanical *** this study,a new method to control the carbon content and avoid the formation ofδ-ferrite by decarburization treatment was *** microstructural changes and mechanical characteristics with carbon content induced by decarburization were systematically ***-Jaoul(C-J)analysis was employed to examine the work hardening characteristics during the tensile *** decarburization by heat treatments,the carbon content within the austenite phase decreased,while Mn and Al were almost unchanged;this made the steel with full austenite transform into the austenite and ferrite dual ***,(Ti,V)C carbides existed in both matrix phase and the mole fraction almost the *** addition,the formation of other carbides *** loss induced a decrease in strength due to the weakening of the carbon solid *** the steel with the single austinite,the deformation mode of austenite was the dislocation planar glide,resulting in the formation of *** the dual-phase steel,the deformation occurred by the dislocation planar glide of austenite first,with the increase in strain,the cross slip of ferrite took place,forming dislocation cells in *** the late stage of deformation,the work hardening of austinite increased rapidly,while that of ferrite increased slightly.

关键词： low-density steels carbon content decarburization strengthening mechanisms work hardening behavior

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Cloning and characterization of a vacuolar cation/proton antiporter in sweet potato（Ipomoea batatas）

Cloning and characterization of a vacuolar cation/proton ant...

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第六届中国植物逆境生理学与分子生物学学术研讨会

作者： weijuan fan and Peng Zhang SIBS-ETH Shanghai Center for Cassava Biotechnology,Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,300 Fenglin Road,Shanghai 200032,China

＜正＞Sweet potato is a herbaceous perennial root crop and has been considered as an important source of food products and biofuels in China because of its stable yield and high tolerance to drought,salinity and infertile *** increase our understanding of the mechanism of salinity resistance in sweet potato,a vacuole Na+/H+antiporter gene,named IbNHX2,was identified by differential screening of a sweet potato cDNA *** IbNHX2cDNA is2458b

关键词： sweet potato Na+/H+ antiporter gene salt resistance functional analysis

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