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Rumen-protected lysine supplementation improved amino acid balance,nitrogen utilization and altered hindgut microbiota of dairy cows

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Animal Nutrition 2023年第4期15卷 320-331页

作者： Xiaoshi Wei Hao Wu Zixiang wang Jinpeng Zhu weijie wang Junhong wang Yanming wang Chong wang College of Animal Science and Technology&College of Veterinary Medicine Zhejiang A&F UniversityHangzhouZhejiang 311300China College of Animal Science Zhejiang UniversityHangzhou 310058China Kemin(China)Technologies Co.Ltd. Zhuhai 519040China

This study was conducted to evaluate the effects of dietary crude protein(CP)and rumen-protected lysine(RPL)supplementation on lactation performance,amino acid(AA)balance,nitrogen(N)utilization and hindgut microbiota in dairy cows.Treatments were in a 2×2 factorial arrangement,and the main effects were CP concentration(16%vs.18%)and RPL supplementation(with or without RPL at40 g/cow per day).Forty cows were randomly allocated to 4 groups:low-CP diet(LP),low-CP diet plus RPL(LPL),high-CP diet(HP),high-CP diet plus RPL(HPL).The experiment was conducted for 8 weeks.Results showed that RPL increased the dry matter intake(P<0.01),milk protein yield(P=0.04)and energy corrected milk(P=0.04),and tended to increase milk fat yield(P=0.06)and fat corrected milk(P=0.05).Cows in the HP group tended to have higher milk urea N(P=0.07).Plasma concentrations of Arg,Ile,Lys,Met,Pro,total essential AA and total nonessential AA were increased by RPL(P<0.05).The total essential AA,total nonessential AA and most AA(except Ile,Phe,Gly and Pro)were increased in the HP group(P<0.05).N excretion was increased in the HP group through an increase in urea N excretion(P<0.01)and an upward trend in plasma urea N(P=0.07).In addition,RPL tended to increase milk protein N secretion(P=0.08),milk N(P=0.07)and microbial protein synthesis(P=0.06),and decreased plasma urea N(P<0.001).In the hindgut,the bacterial community were different between the LP and LPL groups(P<0.01).The probiotic abundances of Christensenellaceae_R-7_group and Acinetobacter were increased by RPL(P=0.03 and 0.03,respectively).The pathogenic abundances of Clostridium_sensu_stricto_1(P<0.001)and Turicibacter(P<0.01)were decreased by RPL.In conclusion,supplementing RPL with low dietary CP could balance AA supply and increase milk protein yield,resulting in an improvement in N utilization efficiency,and altered the composition of the hindgut microbiota to favor the lactation performance of dairy cows.

关键词： Rumen-protected lysine Milk protein N utilization Hindgut microbiota Dairy cow

Construction and application of bioinspired nanochannels based on two-dimensional materials

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Chinese Chemical Letters 2022年第5期33卷 2291-2300页

作者： Jinlin Hao weijie wang Jiawei Zhao Honglin Che Lu Chen Xin Sui College of Materials Science and Engineering Qingdao UniversityQingdao 266071China

With the development of nanotechnology and materials science,bioinspired nanochannels appeared by mimicking the intelligent functions of biological ion channels.They have attracted a great deal of at-tention in recent years due to their controllable structure and tunable chemical properties.Inspired by the layered microstructure of nacre,2D layered materials as excellent matrix material of nanochannel come into our field of vision.Bionic nanochannels based on 2D materials have the advantages of facile preparation,tunable channel size and length,easy expansion,and modification,etc.Therefore,the 2D layered nanofluid system based on bionic nanochannels from 2D layered materials has great potential in biomimetic microsensors,membrane separations,energy conversion,and so on.In this paper,we focus on the construction and application of bionic nanochannels based on 2D layer materials.First,a basic understanding of nanochannels based on 2D materials is briefly introduced,we also present the property of the 2D materials and construction strategies of bionic nanochannels.Subsequently,the application of these nanochannels in responsive channels and energy conversion is discussed.The unsolved challenges and prospects of 2D materials-based nanochannels are proposed in the end.

关键词： Bioinspired nanochannels 2D material Layered structure Energy conversion Responsive nanochannels

Carbon nanofibers membrane bridged with graphene nanosheet and hyperbranched polymer for high-performance osmotic energy harvesting

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Nano Research 2023年第1期16卷 1205-1211页

作者： weijie wang Jinlin Hao Qian Sun Minqi Zhao Huiyi Liu Chao Li Xin Sui College of Materials Science and Engineering Qingdao UniversityQingdao 266071China Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education Key Laboratory of Beijing EnergySchool of Chemistry and EnvironmentBeihang UniversityBeijing 100191China

Reverse electrodialysis(RED),based on ion-selective membranes,is one of the most promising technologies for capturing osmotic energy.As key elements of the RED system,ion-selective membranes must meet the crucial demands of mechanical stability,anti-fouling characteristics,easy fabrication,and high power density;however,this still remains a challenge.In this study,we demonstrated a large-scale,mechanically stable,and high-porosity membrane obtained by combining carbon nanomaterials and hyperbranched polyethyleneimine(h-PEI),thereby achieving a high power density of 5.0 W·m−2 with seawater and river water.Carbon nanofibers(CNFs)were subsequently bridged with graphene and h-PEI to strengthen the interaction between the CNFs,reduce the channel size and increase the space charge density,mechanical strength,and toughness.The large-scale and mechanically stable membrane fabricated using the modified CNFs exhibited anion selectivity and high ionic conductivity,thereby achieving a high-performance osmotic energy conversion.Furthermore,the anti-fouling property of the membrane was confirmed by the stability of the osmotic energy conversion in a solution with algae,which can be attributed to the high porosity of carbon nanomaterials.This economic and convenient method for the ion-selective membrane preparation is believed to be promising for large-scale osmotic energy harvesting.

关键词： carbon nanofibers graphene nanosheet bridged structure hyperbranched polymer osmotic energy harvesting

A pair of G-type lectin receptor-like kinases modulates nlp20-mediated immune responses by coupling to the RLP23 receptor complex

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Journal of Integrative Plant Biology 2023年第5期65卷 1312-1327页

作者： Yazhou Bao Yixin Li Qin Chang Rubin Chen weijie wang Qian Zhang Shuxian Chen Guangyuan Xu Xiaodan wang Fuhao Cui Daolong Dou Xiangxiu Liang MOA Key Laboratory of Pest Monitoring and Green Management Department of Plant PathologyCollege of Plant ProtectionChina Agricultural UniversityBeijing100193China College of Plant Protection Nanjing Agricultural UniversityNanjing 210095China Collegeof LifeSciences SouthChinaAgricultural UniversityGuangzhou 510642China

Plant cells recognize microbial patterns with the plasma-membrane-localized pattern-recognition receptors consisting mainly of receptor kinases(RKs) and receptor-like proteins(RLPs). RKs, such as bacterial flagellin receptor FLS2, and their downstream signaling components have been studied extensively. However, newly discovered regulatory components of RLP-mediated immune signaling, such as the nlp20 receptor RLP23, await identification. Unlike RKs, RLPs lack a cytoplasmic kinase domain, instead recruiting the receptor-like kinases(RLKs) BAK1 and SOBIR1. SOBIR1 specifically works as an adapter for RLP-mediated immunity. To identify new regulators of RLP-mediated signaling, we looked for SOBIR1-binding proteins(SBPs) in Arabidopsis thaliana using protein immunoprecipitation and mass spectrometry,identifying two G-type lectin RLKs, SBP1 and SBP2, that physically interacted with SOBIR1.SBP1 and SBP2 showed high sequence similarity,were tandemly repeated on chromosome 4, and also interacted with both RLP23 and BAK1. sbp1 sbp2 double mutants obtained via CRISPR-Cas9 gene editing showed severely impaired nlp20-induced reactive oxygen species burst, mitogenactivated protein kinase(MAPK) activation, and defense gene expression, but normal flg22-induced immune responses. We showed that SBP1 regulated nlp20-induced immunity in a kinase activityindependent manner. Furthermore, the nlp20-induced the RLP23–BAK1 interaction, although not the flg22-induced FLS2–BAK1 interaction, was significantly reduced in sbp1 sbp2. This study identified SBPs as new regulatory components in RLP23 receptor complex that may specifically modulate RLP23-mediated immunity by positively regulating the interaction between the RLP23 receptor and the BAK1 co-receptor.

关键词： immune responses pattern-recognition receptors receptor-like proteins SBPs SOBIR1

HBV precore G1896A mutation promotes growth of hepatocellular carcinoma cells by activating ERK/MAPK pathway

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Virologica Sinica 2023年第5期38卷 680-689页

作者： Baoxin Zhao Hongxiu Qiao Yan Zhao Zhiyun Gao weijie wang Yan Cui Jian Li Zhanjun Guo Xia Chuai Sandra Chiu Institute of Medical and Health Science Hebei Medical UniversityShijiazhuang050017China Department of Pathogen Biology Hebei Medical UniversityShijiazhuang050017China Department of Gastroenterology and Hepatology The Fourth Hospital of Hebei Medical UniversityShijiazhuang050000China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega ScienceChinese Academy of SciencesWuhan430207China Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei230026China

Chronic hepatitis B virus(HBV)infection is one of the leading causes of hepatocellular carcinoma(HCC).The HBV genome is prone to mutate and several variants are closely related to the malignant transformation of liver disease.G1896A mutation(G to A mutation at nucleotide 1896)is one of the most frequently observed mutations in the precore region of HBV,which prevents HBeAg expression and is strongly associated with HCC.However,the mechanisms by which this mutation causes HCC are unclear.Here,we explored the function and molecular mechanisms of the G1896A mutation during HBV-associated HCC.G1896A mutation remarkably enhanced the HBV replication in vitro.Moreover,it increased tumor formation and inhibited apoptosis of hepatoma cells,and decreased the sensitivity of HCC to sorafenib.Mechanistically,the G1896A mutation could activate ERK/MAPK pathway to enhanced sorafenib resistance in HCC cells and augmented cell survival and growth.Collectively,our study demonstrates for the first time that the G1896A mutation has a dual regulatory role in exacerbating HCC severity and sheds some light on the treatment of G1896A mutation-associated HCC patients.

关键词： HBV G1896A mutation Hepatocellular carcinoma(HCC) Proliferation Apoptosis ERK/MAPK

Defining Proximity Proteome of Histone Modifications by Antibody-mediated Protein A-APEX2 Labeling

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 87-100页

作者： Xinran Li Jiaqi Zhou Wenjuan Zhao Qing Wen weijie wang Huipai Peng Yuan Gao Kelly J.Bouchonville Steven M.Offer Kuiming Chan Zhiquan wang Nan Li Haiyun Gan Shenzhen Key Laboratory of Synthetic Genomics Guangdong Provincial Key Laboratory of Synthetic GenomicsCAS Key Laboratory of Quantitative Engineering BiologyShenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Cold Spring Harbor Laboratory Cold Spring HarborNY 11724USA Department of Molecular Pharmacology and Experimental Therapeutics Mayo ClinicRochesterMN 55905USA Mayo Clinic College of Medicine RochesterMN 55905USA Mayo Clinic Cancer Center RochesterMN 55905USA Department of Biomedical Sciences City University of Hong KongHong Kong Special Administrative Region 999077China Key Laboratory of Biochip Technology Biotech and Health CentreShenzhen Research Institute of City University of Hong KongShenzhen 518172China Division of Hematology Department of MedicineMayo ClinicRochesterMN 55905USA

Proximity labeling catalyzed by promiscuous enzymes,such as APEX2,has emerged as a powerful approach to characterize multiprotein complexes and protein-protein interactions.However,current methods depend on the expression of exogenous fusion proteins and cannot be applied to identify proteins surrounding post-translationally modified proteins.To address this limitation,we developed a new method to label proximal proteins of interest by antibody-mediated protein A-ascorbate peroxidase 2(pA-APEX2) labeling(AMAPEX).In this method,a modified protein is bound in situ by a specific antibody,which then tethers a pA-APEX2 fusion protein.Activation of APEX2 labels the nearby proteins with biotin;the biotinylated proteins are then purified using streptavidin beads and identified by mass spectrometry.We demonstrated the utility of this approach by profiling the proximal proteins of histone modifications including H3 K27 me3,H3 K9 me3,H3 K4 me3,H4 K5 ac,and H4 K12 ac,as well as verifying the co-localization of these identified proteins with bait proteins by published ChIP-seq analysis and nucleosome immunoprecipitation.Overall,AMAPEX is an efficient method to identify proteins that are proximal to modified histones.

关键词： Proximity labeling Post-translationally AMAPEX Modified histone

Direct infection of SARS-CoV-2 in human iPSC-derived 3D cardiac organoids recapitulates COVID-19 myocarditis

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Virologica Sinica 2023年第6期38卷 971-974页

作者： weijie wang Jinxuan Yang Pinfang Kang Jinsong Bai Xiaoli Feng Liuqi Huang Yiming Zhang Yuechun Wu Bi Tang Hongju wang JianJie Jiang Minghua Li Bing Zhao Xinglou Yang Department of Cardiovascular Disease The First Affiliated Hospital of Bengbu Medical CollegeBengbu233004China Yunnan Key Laboratory of Biodiversity Information Kunming Institute of ZoologyChinese Academy of SciencesKunming650201China State Key Laboratory of Genetic Engineering School of Life SciencesZhongshan HospitalFudan UniversityShanghai200438China Kunming Third People's Hospital Kunming650041China Kunming National High-level Biosafety Research Center for Non-human Primates Kunming Institute of ZoologyChinese Academic of SciencesKunming650107China Institute of Organoid Technology Kunming Medical UniversityKunming650500China

Dear editor,The pandemic of coronavirus disease-19(COVID-19)has lasted for more than three years,causing lots of extra death and posing a serious threat to public health.This pandemic swept the last global safety island in a matter of months,coinciding with the switch of zero-COVID policy of China.The causative agent,SARS-CoV-2,spreads globally and then lurks in the population with sporadic outbreaks in local areas.Meanwhile,the sequelae of convalescent COVID-19 patients receded slowly.The pantissue tropism of SARS-CoV-2 results in a wide range of symptoms in patients,including cardiovascular disorders.Cardiac complications occur in 20%–44%of hospitalized patients,which is an independent risk factor for COVID-19 mortality(Ma et al.,2022;Patone et al.,2022).Survivors of acute COVID-19 also have a substantial risk of cardiovascular disease burden lasting up to one year(Xie et al.,2022).

关键词： cardiovascular mortality acute

Photo-responsive Behaviors of Hydrogen-Bonded Polymer Complex Fibers Containing Azobenzene Functional Groups

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Advanced Fiber Materials 2021年第3期3卷 172-179页

作者： Miaomiao Zhu weijie wang Caihong Zhang Liping Zhu Shuguang Yang State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Center for Advanced Low-Dimension MaterialsCollege of Materials Science and EngineeringDonghua UniversityShanghai 201620People’s Republic of China

Two azobenzene compounds,Disperse Red 1(DR1)and 4-aminoazobenzene(Aazo),were separately linked to poly(acrylic acid)(PAA)side chains to form PAA-DR1 and PAA-Aazo,which were then associated with poly(ethylene oxide)(PEO)to produce hydrogen-bonded polymer complex fibers.After UV irradiation,the initial modulus and yielding strength of PAA-Aazo/PEO fiber were both increased tremendously,while the mechanical properties of PAA-DR1/PEO fiber only slightly changed.After drawn and dried in vacuum,PAA-DR1/PEO and PAA-Aazo/PEO fibers with an extended length exhibited a contraction of 20%and 25%under UV irradiation,showing potential for photo-actuation.

关键词： Photo actuator Photomechanical Optomechanical Polymer complex Azobenzene

基于磁性液体的磁浮式振动俘能器系统建模与输出性能分析

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振动与冲击 2024年第1期43卷 218-229页

作者：张宪文李国正王伟杰苏树强北京交通大学机械与电子控制工程学院北京100044

针对现有振动俘能器输出功率低、工作频带范围小等问题,提出了一种基于磁性液体的非线性磁浮式振动俘能器新型结构。提出新型俘能器的结构形式,分析其工作原理,建立磁偶极子非线性力学模型,对比仿真结果与试验数据,验证其准确性;分析磁... 详细信息

针对现有振动俘能器输出功率低、工作频带范围小等问题,提出了一种基于磁性液体的非线性磁浮式振动俘能器新型结构。提出新型俘能器的结构形式,分析其工作原理,建立磁偶极子非线性力学模型,对比仿真结果与试验数据,验证其准确性;分析磁性液体的二阶浮力和黏性阻尼,建立其数学模型,运用多尺度变换方法求解俘能器动力学方程的近似解,进而获得输出性能响应模型;分析三类系统结构参数和激励加速度变化对俘能器输出性能的影响,对比了有无磁性液体时俘能器的输出性能。结果表明:磁铁间距、悬浮磁铁与磁性液体的总质量和激励加速度能有效地改变俘能器的工作频率范围和输出功率幅值,而阻尼比主要影响输出功率幅值。新型俘能器输出电压峰峰值和输出功率峰值分别达到426.88 mV和0.56 mW,增加磁性液体后输出电压和功率分别提升了22.67%和75%,对比其它低频振动俘能器,具有较优的输出功率特性。新型磁浮式俘能器的结构形式与建立的动力学模型能为拓宽此类俘能器工作频带范围和增强输出功率性能提供新的解决思路,具有良好的参考价值。

关键词：振动俘能非线性磁性液体多尺度变换法输出性能