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Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China,India and the US using simple sequence repeat markers

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Journal of Integrative Plant Biology 2016年第5期58卷 452-465页

作者： Hui Wang Pawan Khera Bingyan.Huang Mei Yuan Ramesh Katam weijian zhuang Karen Harris-Shultz Kim M.Moore Albert K.Culbreath Xinyou Zhang Rajeev K.Varshney Lianhui Xie Baozhu Guo Fujian Agricultural and Forestry University College of Plant ProtectionFuzhou 350002China Department of Plant Pathology University of GeorgiaTiftonGA 31794USA USDA-ARS Crop Protection and Management Research UnitTiftonGA 31794USA Shandong Peanut Research Institute qingdao 266100China International Crops Research Institute for the Semi-Arid Tropics(ICRISAT) Patancheru 502324India Henan Academy of Agricultural Sciences Cash Crops Research InstituteZhengzhou 450002China Department of Biological Sciences Florida A&M UniversityTallahasseeFL 32307USA Fujian Agricultural and Forestry University College of Crop ScienceFuzhou 350002China USDA-ARS Crop Genetics and Breeding Research UnitTiftonGA 31794USA AgResearch Consultants Shingler Little River RoadSumnerGA 31789USA

Cultivated peanut is grown worldwide as rich- source of oil and protein. A broad genetic base is needed for cultivar improvement. The objectives of this study were to develop highly informative simple sequence repeat （SSR） markers and to assess the genetic diversity and popuJation structure of peanut cultivars and breeding lines from different breeding programs in China, India and the US. A total of 111 SSR markers were selected for this study, resulting in a total of 472 alleles. The mean values of gene diversity and polymorphic information content （PIC） were 0.480 and o.429, respectively. Country-wise analysis revealed that alleles per locus in three countries were similar. The mean gene diversity in the US, China and India was 0.363, o.489 and 0.47 with an average PIC of 0.323, 0.43 and o.412, respectively. Genetic analysis using the STRUCTURE divided these peanut lines into two populations （P1, P2）, which was consistent with the dendro- gram based on genetic distance （G1, G2） and the clustering of principal component analysis. The groupings were related to peanut market types and the geographic origin with a few admixtures. The results could be used by breeding programs to assess the genetic diversity of breeding materials to broaden the genetic base and for molecular genetics studies.

关键词： Arachis hypogaea breeding genetic diversity populationstructure simple sequence repeat

甘蔗茎解剖生理研究Ⅰ.不同品种茎贮藏组织解剖特征及其对糖分的影响

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作物学报 1989年第4期15卷 369-377,T002页

作者：庄伟建福建农学院

用细胞离析法和冰冻切片技术,研究了10个甘蔗品种的茎贮藏细胞和维管束的解剖特征。甘蔗不同品种茎贮藏细胞体积存在极显著差异。所有品种茎贮藏组织皆有柱状和球状两种贮藏薄壁细胞,高糖品种这两种细胞各约占50%。茎贮藏细胞体积跟蔗... 详细信息

用细胞离析法和冰冻切片技术,研究了10个甘蔗品种的茎贮藏细胞和维管束的解剖特征。甘蔗不同品种茎贮藏细胞体积存在极显著差异。所有品种茎贮藏组织皆有柱状和球状两种贮藏薄壁细胞,高糖品种这两种细胞各约占50%。茎贮藏细胞体积跟蔗糖含量存在极显著负相关、跟锤度存在显著负相关,尤以后期的细胞体积跟蔗糖含量的负相关关系最密切。随着生育期的进展,各个品种的茎贮藏细胞体积逐渐变小,而且有早熟品种变小较快;迟熟品种变小较慢的趋势。不同品种茎维管束密度存在极显著差异,维管束密度与蔗糖含量存在显著正相关,而与细胞体积没有关系。指出茎贮藏细胞小的品种蔗糖含量高、可能是由于茎组织细胞壁、细胞膜和液泡膜的总表面积大,而使吸收面积大,同时茎组织无阻空间大,因而吸收糖量大。说明糖分高低、是受细胞体积大小的影响。细胞体积随生育期的变化速率,也可能影响甘蔗糖分的含量。本研究结果为甘蔗育种提供参考依据。

关键词：甘蔗贮藏细胞组织解剖糖分

Preliminary identification of the function of Ralstonia solanacearum effector protein Rip75 and screening of candidate interaction proteins

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Preliminary identification of the function of Ralstonia sola...

China’s 10-year progress in DC gas-insulated equipment:From basic research to industry perspective

中国植物病理学会2021年学术年会

作者： HEN Kun LI Huaqi GAO Meijia WANG Shanshan DANG Hao WEI Jiaxian zhuang weijian College of Plant Protection Fujian Agriculture and Forestry University College of Agronomy Fujian Agriculture and Forestry University

Bacterial wilt caused by Ralstonia solanacearum is harmful to many *** pathogen caused water shortage due to blocking effect after the propagation of plant vascular bundle,which affected the normal growth and development of plants,and caused the wilt and death,resulting in crop production reduction and huge economic *** the process of pathogen infection,the bacterial use type Ⅲ secretion system to inject a large number of effector proteins directly into host cells,which may affect the immune response of plants and lead to the host plant infected or non-host plants to have hypersensitive *** is a complex species,which can secrete more than 100 kinds of effector proteins,but the function of most of them is not *** whole genome of *** has been sequenced in this laboratory,and 75 effector proteins were predicted by T3 Es *** this study,we selected Rip75 as candidate effector protein gene to clone and preliminarily analyze the ***75,which encodes a total of 338 amino acids and has no conserved domain,can cause hypersensitive response in Nicotiana *** is a new effector protein that has not been reported in *** the mutant strain of Δrip75 was constructed by homologous *** inoculating peanut varieties resistant and susceptible to bacterial wilt,it was found that the pathogenicity of mutant strain Δrip75 to peanut was *** proteins（corresponding to AhPRPS,AhKUAl and AhSBT） interacting with Rip75 were screened by yeast two-hybrid method,and their interaction was verified by BIFC *** results preliminarily revealed the function of the effector protein Rip75 and speculated that it may be a toxicprotein,which provided a theoretical basis for the resistance and control of peanut bacterial wilt.

关键词： Ralstonia solanacearum Peanut Rip75 Effector Yeast two-hybrid

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iEnergy

iEnergy 2022年第4期1卷 400-433页

作者： Chuanyang Li Changhong Zhang Jinzhuang Lv Fangwei Liang Zuodong Liang Xianhao Fan Uwe Riechert Zhen Li Peng Liu Jianyi Xue Cheng Pan Geng Chen Lei Zhang Zheming Wang Wu Lu Hucheng Liang Zijun Pan weijian zhuang Giovanni Mazzanti Davide Fabiani Bo Liu Shaohua Cao Jianying Zhong Yuan Deng Zhenle Nan Jingen Tang Jinliang He State Key Laboratory of Power Systems Department of Electrical EngineeringTsinghua UniversityBeijing 100084China Maintenance&Test Center of EHV Power Transmission Company China Southern Power Grid Guangzhou 510663China EHV Power Transmission Company China Southern Power Grid Guangzhou 510663China Hitachi Energy Switzerland Ltd ZürichSwitzerland Key Laboratory of Engineering Dielectrics and Its Application Ministry of EducationHarbin University of Science and TechnologyHarbin 150080China State Key Laboratory of Electrical Insulation and Power Equipment Xi’an Jiaotong UniversityXi’an 710049China School of Electrical Engineering and Automa-tion Hefei University of TechnologyHefei 230009China School of Electrical Engineering and Automation Wuhan UniversityWuhan 430072China Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources North China Electric Power UniversityBeijing 102206China Department of Electrical Engineering Shanghai University of Electric PowerShanghai 200090China School of Electrical and Information Engineering Tianjin UniversityTianjin 300072China Department of Electrical Electronic and Information EngineeringUniversity of BolognaBologna 40136Italy Shandong Taikai Electrical Insulation Co. Ltd.Tai’an 271000China PingGao Group CO. Ltd.Pingdingshan 467001China China XD Group Co. Ltd.Xi’an 710075China Jiangsu Jinxin Electric Appliance Co. Ltd.Yangzhou 225202China

The construction of the future energy structure of China under the 2050 carbon-neutral vision requires compact direct current(DC)gas-insulation equipment as important nodes and solutions to support electric power transmission and distribution of long-distance and *** paper reviews China's 10-year progress in DC gas-insulated *** progresses in basic research and industry perspective are presented,with related scientific issues and technical bottlenecks being *** progress in DC gas-insulated equipment worldwide(Europe,Japan,America)is also reported briefly.

关键词： DC gas-insulated switchgear(GIS) DC gas-insulated power transmission line(GIL) surface charge flashover metal particle offshore projects

烟草根特异NtR19基因终止子的克隆及功能分析

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分子植物育种 2014年第3期12卷 466-471页

作者：蔡翠雅庄宇慧张冲曾缘欢陈顺辉庄伟建福建农林大学作物科学学院福州350002 福建省作物分子与细胞生物学重点实验室福州350002 湖南省湖南农业大学生物科学技术学院长沙410000 福建省烟草专卖局烟草农业科学研究所福州350003

为了开发具有自主知识产权的且来源于烟草的终止子,本研究根据烟草根特异NtR19基因的3'UTR序列设计引物,从烟草基因组中克隆并获得了该基因的终止子(T19),并构建植物表达载体转化翠碧一号烟草,通过分子检测和GUS染色验证了NtR19基因终... 详细信息

为了开发具有自主知识产权的且来源于烟草的终止子,本研究根据烟草根特异NtR19基因的3'UTR序列设计引物,从烟草基因组中克隆并获得了该基因的终止子(T19),并构建植物表达载体转化翠碧一号烟草,通过分子检测和GUS染色验证了NtR19基因终止子具有转录终止的功能,同时也说明NtR19基因全长mRNA的3'UTR可以作为终止子加以利用,使用来自烟草的终止子能在一定程度上解决外源基因安全性的研究,这对转基因的安全性提供了资源基础。

关键词：烟草 NtR19 终止子载体构建 GUS染色

水稻隐花色素基因Cry1a/Cry2/Cry1b RNAi表达载体构建及遗传转化研究

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热带作物学报 2011年第10期32卷 1843-1848页

作者：李毓戴飞王乃元杨小飞庄伟建仇秀丽福建农林大学作物科学学院福建福州350002 福建农林大学作物遗传育种与综合利用教育部重点实验室福建福州350002 福建省分子与细胞生物学重点实验室福建福州350002

构建一个包含3个水稻隐花色素基因片段的RNAi表达载体pSC1301-347-Cry1aCry2Cry1b并转化水稻,以期导致Cry1a、Cry1b与Cry2集体沉默,观察它们对水稻重要农艺性状的影响。结果表明,转基因水稻开花期显著延迟,结实率骤降,株高增加,剑叶变短... 详细信息

构建一个包含3个水稻隐花色素基因片段的RNAi表达载体pSC1301-347-Cry1aCry2Cry1b并转化水稻,以期导致Cry1a、Cry1b与Cry2集体沉默,观察它们对水稻重要农艺性状的影响。结果表明,转基因水稻开花期显著延迟,结实率骤降,株高增加,剑叶变短,谷粒变长、变宽;而剑叶宽、穗长与对照无显著差异。

关键词：水稻隐花色素基因 RNAi 载体构建遗传转化

青枯菌诱导的烟草叶片全长cDNA文库的构建和初步分析

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分子植物育种 2013年第5期11卷 624-629页

作者：张冲蔡铁城陈华曾建斌庄伟建福建农林大学生命科学学院福州350002 福建省作物分子与细胞生物学重点实验室福州350002

以优质高抗青枯病烤烟品种K326为材料，在苗期利用注射法接种烟草青枯菌，分不同时期取叶片，利用CTAB法提取接种和非接种的混合RNA，采用SMART（switching mechanism at 5’end of RNA transcript）技术合成双链cDNA，经Sfi Ⅰ酶切后... 详细信息

以优质高抗青枯病烤烟品种K326为材料，在苗期利用注射法接种烟草青枯菌，分不同时期取叶片，利用CTAB法提取接种和非接种的混合RNA，采用SMART（switching mechanism at 5’end of RNA transcript）技术合成双链cDNA，经Sfi Ⅰ酶切后胶回收纯化双链cDNA，连接到质粒载体pDNR—LIB上，电击转化大肠杆菌DH5α感受态细胞，成功构建了青枯菌诱导的烟草叶片全长cDNA文库。经鉴定，初级文库库容为1．902×10^6cfu／mL，重组率达到98％以上，插入片段集中在0．75～2．0kb之间，平均长度约为1300bp，经扩增后的文库滴度为2．97×10^9cfu／mL。随机挑取部分克隆测序，利用生物信息学软件进行初步分析获得部分EST数据。结果显示得到了高质量的全长cDNA文库，这为筛选和克隆青枯菌诱导的胁迫相关基因提供了资源基础。

关键词：烟草青枯病 SMART技术 cDNA文库

花生预苯胺酸脱氢酶基因AhADH的克隆及分子特性分析

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分子植物育种 2014年第3期12卷 478-484页

作者：张福婷周双彪张冲陈华蔡铁城庄伟建福建农林大学食品科学学院福州350002 福建省作物分子与细胞生物学重点实验室福州350002

通过改良的RACE技术从花生中分离并克隆出ADH全长cDNA序列,并命名为AhADH,其全长为1 155 bp,含一个编码269个氨基酸,长度为810 bp的开放阅读框。生物信息学分析显示AhADH蛋白的分子质量是30.65 kD,理论等电点是6.07,二级结构主要由α-... 详细信息

通过改良的RACE技术从花生中分离并克隆出ADH全长cDNA序列,并命名为AhADH,其全长为1 155 bp,含一个编码269个氨基酸,长度为810 bp的开放阅读框。生物信息学分析显示AhADH蛋白的分子质量是30.65 kD,理论等电点是6.07,二级结构主要由α-螺旋和无规卷曲组成。序列比对表明花生ADH氨基酸序列含预苯酸/预苯胺酸脱氢酶结构域,且与多种其他植物的ADH具有高度同源性。qRT-PCR分析表明该基因具有组织表达差异性,且在茎中表达量最高。

关键词：维生素E 预苯胺酸脱氢酶花生生物信息学分析组织表达谱

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花生组织培养研究进展

福建农林大学学报（自然科学版） 1999年第3期30卷 268-273页

作者：张书标庄伟建刘思衡福建农业大学农业部甘蔗遗传育种重点开放实验室福州350002 福建农业大学作物学院福州350002

花生组织培养对于品种改良、新品种繁殖、遗传转化和种质保存等具有重要意义．本文概述了花生苗的再生培养、体细胞胚的诱导培养、花药培养、远缘杂种胚营救培养、原生质体培养和遗传转化的研究进展。

关键词：花生组织培养苗的再生培养体细胞胚花药培养杂种胚营救培养原生质体培养遗传转化