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加速溶剂萃取-硅胶柱净化-气相色谱-三重四极杆质谱法测定环境空气气态和颗粒态样品中20种卤代多环芳烃

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冶金分析 2024年第1期44卷 35-43页

作者：牛少敏张鑫田渭花李飞王婷杏艳陕西省环境监测中心站陕西省环境介质痕量污染物监测预警重点实验室

环境空气中卤代多环芳烃(HPAHs)的含量多在皮克(pg)级别，且基质干扰大，准确测定其含量难度较大。实验采用体积比为1∶1的正己烷-二氯甲烷为萃取溶剂进行加速溶剂萃取(ASE),用硅胶柱以体积比为3∶2的正己烷-二氯甲烷为洗脱溶剂进行净... 详细信息

环境空气中卤代多环芳烃(HPAHs)的含量多在皮克(pg)级别，且基质干扰大，准确测定其含量难度较大。实验采用体积比为1∶1的正己烷-二氯甲烷为萃取溶剂进行加速溶剂萃取(ASE),用硅胶柱以体积比为3∶2的正己烷-二氯甲烷为洗脱溶剂进行净化，以菲-d10、■-d12、苝-d12为内标进行定量，建立了气相色谱-三重四极杆质谱法(GC-QQQ-MS/MS)同时检测环境空气气态和颗粒态中20种HPAHs的分析方法。实验表明，在0.1～20.0 pg/μL范围内，20种HPAHs的校准曲线线性关系良好，相关系数r>0.995,检出限为0.05～0.4 pg/μL,定量限为0.1～1.5 pg/μL。高、低2个浓度水平的空白加标试验结果表明，低浓度(0.4 pg/μL)时20种HPAHs的平均回收率为75%～113%,相对标准偏差(RSD,n=6)为0～15.6%;高浓度(5.0 pg/μL)时20种HPAHs的平均回收率为82%～97%,RSD(n=6)为1.8%～5.8%,符合标准HJ 646—2013对母体多环芳烃(PAHs)空白加标回收率和相对标准偏差的相关规定。将实验方法应用于样品和样品加标分析，测得环境空气气态和颗粒态样品中20种HPAHs浓度范围为未检出到2.0 pg/μL;当加标浓度为3.0 pg/μL时，20种HPAHs的平均加标回收率为80%～107%,RSD(n=6)为2.9%～8.2%。

关键词：卤代多环芳烃(HPAHs) 加速溶剂萃取(ASE) 气相色谱-三重四极杆质谱法(GC-QQQ-MS/MS) 环境空气气态颗粒态

Integrative analysis of miRNA and mRNA profiles reveals that gga-miR-106-5p inhibits adipogenesis by targeting the KLF15 gene in chickens

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1671-1689页

作者： weihua tian Xin Hao Ruixue Nie Yao Ling Bo Zhang Hao Zhang Changxin Wu National Engineering Laboratory for Animal Breeding Beijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Hainan 572025 SanyaChina.

Background:Excessive abdominal fat deposition in commercial broilers presents an obstacle to profitable meat quality,feed utilization,and *** fat deposition depends on the proliferation of preadipocytes and their maturation into adipocytes,which involves a cascade of regulatory *** evidence has shown that microRNAs(miRNAs)serve as post-transcriptional regulators of adipogenic differentiation in ***,the miRNA-mediated molecular mechanisms underlying abdominal fat deposition in chickens are still poorly *** study aimed to investigate the biological functions and regulatory mechanism of miRNAs in chicken abdominal ***:We established a chicken model of abdominal adipocyte differentiation and analyzed miRNA and mRNA expression in abdominal adipocytes at different stages of differentiation(0,12,48,72,and 120 h).A total of 217 differentially expressed miRNAs(DE-miRNAs)and 3520 differentially expressed genes were *** prediction of DE-miRNAs and functional enrichment analysis revealed that the differentially expressed targets were significantly enriched in lipid metabolism-related signaling pathways,including the PPAR signaling and MAPK signaling pathways.A candidate miRNA,gga-miR-106-5p,exhibited decreased expression during the proliferation and differentiation of abdominal preadipocytes and was downregulated in the abdominal adipose tissues of fat chickens compared to that of lean ***-miR-106-5p was found to inhibit the proliferation and adipogenic differentiation of chicken abdominal preadipocytes.A dual-luciferase reporter assay suggested that the KLF15 gene,which encodes a transcriptional factor,is a direct target of ***-miR-106-5p suppressed the posttranscriptional activity of KLF15,which is an activator of abdominal preadipocyte proliferation and differentiation,as determined with gain-and loss-of-function ***:gga-miR-106-5p function

关键词： Abdominal fat Adipogenesis Chickens gga-miR-106-5p KLF15 MiRNA

CircDOCK7 facilitates the proliferation and adipogenic differentiation of chicken abdominal preadipocytes through the gga‑miR‑301b‑3p/ACSL1 axis

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2290-2315页

作者： weihua tian Ye Liu Wenhui Zhang Ruixue Nie Yao Ling Bo Zhang Hao Zhang Changxin Wu National Engineering Laboratory for Animal Breeding Beijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background Abdominal fat deposition depends on both the proliferation of preadipocytes and their maturation into adipocytes,which is a well-orchestrated multistep process involving many regulatory *** RNAs(circRNAs)have emergingly been implicated in mammalian ***,circRNA-mediated regulation in chicken adipogenesis remains *** previous circRNA sequencing data identified a differentially expressed novel circRNA,8:27,886,180|27,889,657,during the adipogenic differentiation of chicken abdominal *** study aimed to investigate the regulatory role of circDOCK7 in the proliferation and adipogenic differentiation of chicken abdominal preadipocytes,and explore its molecular mechanisms of competing endogenous RNA underlying chicken *** Our results showed that 8:27,886,180|27,889,657 is an exonic circRNA derived from the head-to-tail splicing of exons 19–22 of the dedicator of cytokinesis 7(DOCK7)gene,abbreviated as ***7 is mainly distributed in the cytoplasm of chicken abdominal preadipocytes and is stable because of its RNase R resistance and longer ***7 is significantly upregulated in the abdominal fat tissues of fat chickens compared to lean chickens,and its expression gradually increases during the proliferation and adipogenic differentiation of chicken abdominal ***,the gain-and loss-of-function experiments showed that circDOCK7 promoted proliferation,G0/G1-to S-phase progression,and glucose uptake capacity of chicken abdominal preadipocytes,in parallel with adipogenic differentiation characterized by remarkably increased intracellular lipid droplet accumulation and triglyceride and acetyl coenzyme A content in differentiated chicken abdominal ***,a pull-down assay and a dual-luciferase reporter assay confirmed that circDOCK7 interacted with gga-miR-301b-3p,which was identified as an inhibitor of chicken abdominal adipogene

关键词： Abdominal fat deposition Adipogenesis Chickens CircDOCK7 Competing endogenous RNA MiRNA sponge

CD14基因启动子区-159C/T多态性在H型高血压患者中的分布

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甘肃医药 2024年第2期43卷 107-110页

作者：田卫花周思彤侯娟娟张丹马剑华甘肃省中医院甘肃兰州730050 甘肃省中医药研究院甘肃兰州730050 庆阳市人民医院甘肃庆阳744500 兰州大学公共卫生学院甘肃兰州730000

目的:调查H型高血压患者中CD14基因启动子区-159C/T基因多态性分布情况。方法:用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术对H型高血压组、普通高血压组和对照组的CD14基因启动子区-159C/T多态性分布进行研究,同时检测血清... 详细信息

目的:调查H型高血压患者中CD14基因启动子区-159C/T基因多态性分布情况。方法:用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术对H型高血压组、普通高血压组和对照组的CD14基因启动子区-159C/T多态性分布进行研究,同时检测血清同型半胱氨酸(HCY)及其他生化指标。结果:H型高血压组CC型、CT型、TT型分布频率分别为10%、43%、47%,C和T等位基因频率分别为31.5%、68.5%;普通高血压组CC型、CT型、TT型分布频率分别为10%、50%、40%,C和T等位基因频率分别为35%、65%;对照组CC型、CT型、TT型分布频率分别为13%、53%、34%,C和T等位基因频率分别为39.5%、60.5%,三组基因分型和等位基因频率均无统计学意义(P>0.05)。与对照组相比,H型高血压组、普通高血压组高密度脂蛋白(HDL-C)水平降低,差异有统计学意义(P<0.05)。结论:H型高血压患者CD14基因启动子区-159C/T多态性分布无明显差异。

关键词： CD14基因启动子基因多态性同型半胱氨酸 H型高血压

NK cells in immunotolerant organs

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Cellular & Molecular Immunology 2013年第3期10卷 202-212页

作者： Haoyu Sun Cheng Sun Zhigang tian weihua Xiao Institute of Immunology School of Life Sciences University of Science and Technology of China Hefei China

Organs such as the liver, uterus and lung possess hallmark immunotolerant features, making these organs important for sustaining self-homeostasis. These organs contain a relatively large amount of negative regulatory immune cells, which are believed to take part in the regulation of immune responses. Because natural killer cells constitute a large proportion of all lymphocytes in these organs, increasing attention has been given to the roles that these cells play in maintaining immunotolerance. Here, we review the distribution, differentiation, phenotypic features and functional features of natural killer cells in these immunotolerant organs, in addition to the influence of local microenvironments on these cells and how these factors contribute to organ-specific diseases.

关键词： immunotolerance liver lung natural killer cell uterus

一种新型改良染色法对血涂片和骨髓涂片细胞染色效果评价

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甘肃医药 2023年第10期42卷 906-908,918页

作者：田卫花甘肃省中医院/甘肃省中医药研究院甘肃兰州730050

目的:探讨用一定比例稀释的刘B溶液(主要成分亚甲蓝)代替传统瑞氏染色中的磷酸盐缓冲液(主要成分磷酸盐),以提高临床检验工作中血涂片和骨髓涂片细胞染色质量和染色效率的实验方法。方法:对血涂片和骨髓涂片细胞染色传统实验方法进行改... 详细信息

目的:探讨用一定比例稀释的刘B溶液(主要成分亚甲蓝)代替传统瑞氏染色中的磷酸盐缓冲液(主要成分磷酸盐),以提高临床检验工作中血涂片和骨髓涂片细胞染色质量和染色效率的实验方法。方法:对血涂片和骨髓涂片细胞染色传统实验方法进行改良,分别用蒸馏水以10倍、15倍、20倍稀释刘B溶液,以代替传统瑞氏染色中的磷酸盐缓冲液(KH2PO4-Na2HPO),用普通光学显微镜在油镜下放大1000倍观察血细胞涂片和骨髓细胞涂片的染色效果。结果:改良染色法与传统细胞染色法相比较,改良后的染色方法可以缩短血涂片和骨髓涂片细胞染色时间(血细胞涂片染色总时间由传统瑞氏染色的15 min缩短为5~7 min;骨髓细胞涂片染色总时间由传统瑞氏染色的30 min缩短为15 min);在普通光学显微镜油镜头下放大1000倍观察新型改良染色方法染色的血细胞涂片和骨髓细胞涂片染色效果和细胞形态结构时发现,各类细胞轮廓、结构清晰,细胞浆与细胞核着色稳定,符合国际血液学标准委员会(ICSH)推荐的染色要求。结论:该新型改良染色方法可提高血涂片和骨髓涂片细胞染色的质量和人工复检的效率,具有很好的推广前景。

关键词：血涂片骨髓涂片瑞氏染色缓冲液染色效果

基于混合加点Kriging代理模型的高速列车头型气动多目标优化

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西南交通大学学报 2024年第1期59卷 46-53页

作者：戴志远李田张卫华张继业西南交通大学轨道交通运载系统全国重点实验室四川成都610031

在高速列车多目标气动优化设计中,结合传统加点准则建立的代理模型在初始抽样样本点较少时寻优效率较低.为提高优化效率,本文融合改善期望加点准则(EIC)及Pareto前沿解加点准则(PIC),提出混合加点准则(HIC)方法,并基于HIC方法建立Krigin... 详细信息

在高速列车多目标气动优化设计中,结合传统加点准则建立的代理模型在初始抽样样本点较少时寻优效率较低.为提高优化效率,本文融合改善期望加点准则(EIC)及Pareto前沿解加点准则(PIC),提出混合加点准则(HIC)方法,并基于HIC方法建立Kriging代理模型,以高速列车头车气动阻力、尾车气动阻力及尾车气动升力最小为优化目标,开展高速列车头型多目标气动优化研究;并以Branin单目标测试函数和Poloni多目标测试函数为例,对比分析EIC、PIC和HIC 3种代理模型的收敛速度.结果表明:单目标优化中,相比于EIC和HIC代理模型,HIC代理模型的寻优效率提高50.0%;多目标优化中,与PIC代理模型相比,HIC代理模型的效率提高62.5%;采用HIC代理模型开展头型多目标气动优化得到的最优解模型与原始模型相比,高速列车头车气动阻力、尾车气动阻力和尾车气动升力分别降低1.6%、1.7%和3.0%;最优解模型的鼻尖高度、车钩区域高度及司机室视窗高度都有所降低,2条横向轮廓线内缩.

关键词：代理模型混合加点准则(HIC) 高速列车气动优化

Automatic generalization of metro maps based on dynamic segmentation

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Science China(Technological Sciences) 2010年第S1期53卷 158-165页

作者： DONG weihua 1,tian Yuan 2 & ZHANG Yi 2 1 Institute of Geography and Remote Sensing,Beijing Normal University,Beijing 100875,China 2 Institute of Remote Sensing and Geographic Information System,Peking University,Beijing 100871,China Institute of Geography and Remote Sensing Beijing Normal University Beijing China Institute of Remote Sensing and Geographic Information System Peking University Beijing China

A metro map is usually optimized for the readability of connections and transportation networks *** order to assure good readability and meet aesthetic considerations,a set of principles for good metro map layout are *** to these principles,a new methodology based on dynamic segmentation is presented to produce the metro maps ***,routes are constructed according to the line attribute similarity and geometry *** a set of cartographic generalization methods about the shape,angle,length,and topology are presented for these *** method is validated by Beijing metro plan *** the experiment results,it can be concluded that this new method is more effective than the static segmentation method to produce metro maps with better readability for route plans.

关键词： metro maps generalization dynamic segmentation visualization

唑来膦酸注射液对2型糖尿病合并骨质疏松患者骨密度的影响及治疗机制研究

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中国医学工程 2023年第8期31卷 83-87页

作者：王顺阁田伟华平顶山市第二人民医院内分泌科河南平顶山467000 平顶山市第二人民医院重症医学科河南平顶山467000

目的观察唑来膦酸注射液对改善2型糖尿病(T2DM)合并骨质疏松患者骨密度的影响,分析其药理机制。方法回顾性分析平顶山市第二人民医院2020年5月至2022年2月收治的131例T2DM合并骨质疏松患者为研究对象,采用抽签法,将入组患者分别列为试验... 详细信息

目的观察唑来膦酸注射液对改善2型糖尿病(T2DM)合并骨质疏松患者骨密度的影响,分析其药理机制。方法回顾性分析平顶山市第二人民医院2020年5月至2022年2月收治的131例T2DM合并骨质疏松患者为研究对象,采用抽签法,将入组患者分别列为试验组(66例)和对照组(65例),对照组仅予以常规治疗,试验组采用唑来膦酸注射液配合治疗,比较两组患者的血糖水平、不同区域骨密度水平、骨代谢水平,对所有患者开展为期6个月的短期随访,评估其用药安全性。结果治疗前,两组患者的血糖、骨密度、骨代谢水平差异均无统计学意义(P>0.05);治疗后,试验组的空腹血糖(FBG)、餐后2 h血糖(2 h FBG)、糖化血红蛋白(HbA1c)水平与对照组比较差异无统计学意义(P>0.05);试验组的Ward三角区、股骨、腰椎骨密度水平高于对照组,骨保护素(OPG)高于对照组,骨特异性碱性磷酸酶(BALP)高于对照组,1型胶原C端β特殊序列(β-CTX)水平低于对照组(P0.05)。结论采用唑来膦酸注射液辅助治疗T2DM合并骨质疏松可在不影响患者的血糖水平基础上,促进其骨密度水平提升及骨代谢情况改善,且联合应用此药未明显增加患者药物副反应发生风险,安全性较高,其药理机制主要体现在对破骨细胞、骨吸收的抑制方面。

关键词： 2型糖尿病骨质疏松唑来膦酸注射液骨密度水平用药安全性