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Upregulated HCN2-mediated signaling contributes to TNBS-induced post-inflammatory hypersensitivity of the murine small intestine

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Upregulated HCN2-mediated signaling contributes to TNBS-indu...

2012国际生理学学术大会

作者： Yingping Wang Biying Sun Qian Li Li Dong Guohua Zhang weifang rong Department of Physiology Shanghai Jiaotong University School of Medicine

We previously demonstrated a differential expression of hyperpolarization-activated cyclic nucleotidegated cation channels,HCN2 and HCN3,in spinal and vagal afferents of the murine jejunum.Here we explore the role of HCNs in TNBS-induced visceral hypersensitivity.Ramp distension(to 60 mmHg) of ex vivo jejunum segments from naive or TNBS-treated(50 mg/kg,1 to 8 weeks prior to experiment) mice both evoked biphasic increases in mesenteric afferent nerve activity,reflecting the activation of low and high threshold fibers.High threshold mechanosensory responses(HTRs) were greater in TNBS-treated than in native *** blocker ZD7288(10μM) inhibited the low threshold mechanosensory responses(LTRs) in naive preparations but in contrast attenuated HTRs in TNBS-treated *** modulator cAMP(1 mM) augmented HTRs in TNBS-treated preparations and was without effect on the mechanosensory activity of naive preparations.PKA activator Sp-cAMPS(30μM) didn’t alter the mechanosensory activity of naive or TNBS-treated preparations.With the presence of PKA inhibitor,Rp-cAMPS(12μM),cAMP still potentiated HTRs of TNBS-treated preparations. These results together with histological data suggest that upregulation of HCN-mediated signaling might contribute to TNBS-induced post-inflammatory small intestinal hypersensitivity.

关键词： HCN spinal afferent vagal afferent mechanosensitivity

Successful conversion of advanced gastric cancer treated by immunotherapy combined with chemotherapy: A case report

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Cancer Advances 2024年第8期7卷 1-6页

作者： Xue-Mei Sun Fu-rong Sun Wei-Wei Zheng Hai Yu Ming-Chen Liu Han-Yi Yao Li-Min Huang Shandong University of Traditional Chinese Medicine Jinan 250013China Department of Spleen and Stomach Diseases Weifang Traditional Chinese Medicine HospitalWeifang 261041China Department of Pathology Weifang Hospital of Traditional Chinese MedicineWeifang 261041China Department of Oncology Weifang Hospital of Traditional Chinese MedicineWeifang 261041China

Gastric cancer is one of the most common malignant tumors,for patients with advanced gastric cancer,the traditional treatment is mainly chemotherapy or combined targeted therapy;however,these have not achieved ideal efficacy.However,with the continuous deepening of research on molecular targeted drugs and immunosuppressants,the treatment of advanced gastric cancer patients has made new progress,and some new technologies have also been continuously emerged and applied,which brings hope for the treatment of advanced gastric cancer.Recently,several multicenter randomized controlled phase III studies on immunotherapy for advanced gastric cancer yielded encouraging results,demonstrating its superior efficacy in the treatment of advanced gastric cancer.However,prospective data to support the acceptance of surgery and the R0 removal rate following conversion therapy with immune checkpoint inhibitors are lacking.In this study,a 58-year-old woman with advanced gastric cancer and positive PD-L1 expression,negative HER-2 expression,and microsatellite stable status received immunochemotherapy combined with traditional Chinese medicine to achieve R0 resection and satisfactory efficacy.

关键词： late gastric cancer conversion therapy immunotherapy Traditional Chinese medicine

GPER-expressing neurons in the RVM play a major role in descending regulation of itch

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GPER-expressing neurons in the RVM play a major role in desc...

2019中国生理学会学术年会暨张锡钧基金第十五届全国青...

作者：高婷 Jiahong Qian Xiaowei Ding Po Gao Guohua Zhang weifang rong Department of Anatomy and Physiology College of Basic Medical SciencesShanghai Jiao Tong University School of Medicine

Itch is an unpleasant cutaneous sensation that triggers a strong urge to scratch.Despite significant breakthroughs in understanding the molecular and cellular mechanisms of itch at the spinal and peripheral levels,little is known about the role of the supraspinal descending system in itch regulation.Here, we report that a neuronal population which specifically express the G protein-coupled estrogen receptor（GPER） in the rostral ventromedial medulla（RVM）, an essential part of the descending pain modulation system, were significantly activated in itch conditions.We found that itch-evoked scratching behavior was impaired after activating GPER+ neurons in the RVM, while ablating or suppressing these neurons increased itch-induced scratching behavior.Moreover, GPER-deficient mice and rats exhibited hypersensitivity to mechanical and chemical itch, a phenotype reversible by μ type opioid receptor（MOR）antagonism.Additionally, significant MOR phosphory lation in the RVM was detected in chronic itch models in wild-type but not in GPER-/-rats.These results indicate that RVM GPER+ neurons might exert an "anti-pruritus" function through regulating opioid signaling pathways.

关键词： itch G protein-coupled estrogen receptor μtype opioid receptor rostral ventromedial medulla

Loss of cell adhesion molecule CHL1 improves homeostatic adaptation and survival in hypoxic stress

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Loss of cell adhesion molecule CHL1 improves homeostatic ada...

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：黄欣 weifang rong Tong Zhao Liying Wu lingling Zhu Ming Fan 军事医学科学院基础医学研究所军事认知研究室

Objective CHL1 is a transmembrane cell adhesion molecule that is critical for brain development and in the maintenance of neural circuits in the adult.Recent studies revealed that CHL1 has diverse roles and is involved in the regulation of recovery after spinal cord injury.CHL1 expression was down-regulated in the cerebral cortex,hypothalamus and brain stem after induction of acute hypoxia.In the current study we sought to address the role of CHL1 in regulating homeostasis responses to hypoxia using CHL1 knockout（CHL1-/-）mice.Results We found that,compared with the wild type littermates,CHL1-/-mice showed a dramatically lower mortality rate and an augmented ventilatory response after they were subjected to acute hypoxia.Immunofluorescence staining revealed that CHL1 was expressed in the carotid body（CB）,the key oxygen sensor in rodents and CHL1 expression level in CB as assayed by western blot was decreased after hypoxic exposure.The number of glomus cells and the expression of tyrosine hydroxylase（TH,a marker for glomus cells）in CB of CHL1-/-mice appeared to be increased compared with CHL1+/+mice.In addition,in the ex vivo carotid body preparation,hypoxia induced a significantly greater afferent nerve discharge in CHL1-/-mice compared with the CHL1+/+mice.Furthermore,the arterial blood pressure and plasma catecholamine level of CHL1-/-mice were also significantly higher than those of CHL1+/+mice.Conclusion Our findings first demonstrate that CHL1 is a novel intrinsic factor that is involved in carotid body function and in the ventilatory response to acute hypoxia.

关键词： CHL1 acute hypoxia homeostatic adaptation survival

Estrogen caused a calcium rise via activation of the G protein-coupled estrogen receptor in SH-SY5Y cells

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Estrogen caused a calcium rise via activation of the G prote...

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者： Xiaowei Ding Po Gao Li Dong Qian Li Guohua Zhang weifang rong Department of Physiology Shanghai Jiaotong University School of Medicine

The G protein-coupled estrogen receptor 1(GPER1,also known as GPR30),is a Gs or Gi/o coupled GPCR and has been reported to bind 17β-estradiol(E2)to mediate the non-genomic estrogenic responses.GPER1 has been detected in several brain regions,including the hypothalamus,the brainstem and the spinal cord.However,the signaling mechanisms of this receptor in neuronal cells are yet to be established.The present study was aimed to test the hypothesis that activation of GPER1 might induce a change in intracellular calcium level in SH-SY5Y cells,a human neuroblastoma cell line.The presence of GPER1 was confirmed by western blot and immunofluorescence assay.Importantly,the receptor appeared to be enriched on the Golgi apparatus,rather than the plasma membrane.We then performed calcium imaging and tested the effects of E2 and G-1,a synthetic GPER1 agonist.Both E2and G-1 evoked rapid(within seconds)and robust calcium rises in concentration-dependent manners(0.01μM for E2 and 0.1μM for G-1).The effects were blocked by the GPER1 antagonist G-15(3μM).In the absence of extracellular calcium,both E2 and G-1 only gave rise to minimal calcium responses.However,Cd Cl2(200μM),a non-selective inhibitor of the voltage-gated calcium channels,failed to block the increase of intracellular calcium induced by E2 or G-1.In contrast,thapsigargin(10μM),which supposedly caused store depletion,prevented the calcium response to E2 and G-1.The IP3R inhibitor,2-APB(100μM),also partially blocked the effects of E2 or G-1.These results demonstrate that activation of GPER1 may induce a rapid calcium rise that appears to be dependent on both calcium entry and calcium release.The channels mediating the calcium influx remain to be determined.The rise of intracellular calcium following activation of GPER1 may underlie some of the rapid effects of estrogen in the central nervous system.

关键词： Estrogen G protein-coupled estrogen receptor intracellular calcium SH-SY5Y cells

陆地生态系统碳收支时空变化及影响因素——以湖南省郴州市为例

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长江流域资源与环境 2024年第5期33卷 1066-1075页

作者：杨卓琳刘荣波逄锦光王姝王诗涵冯徽徽中南大学地球科学与信息物理学院湖南长沙410083 山东省第四地质矿产勘察院山东潍坊261021

陆地生态系统碳收支不仅是衡量地表生态环境的重要参数,也是全球碳循环的关键变量。以国家低碳试点城市、可持续发展创新示范区——湖南省郴州市为研究区域,耦合多源遥感数据,结合土壤呼吸地统计模型、趋势分析、地理探测器和残差分析... 详细信息

陆地生态系统碳收支不仅是衡量地表生态环境的重要参数,也是全球碳循环的关键变量。以国家低碳试点城市、可持续发展创新示范区——湖南省郴州市为研究区域,耦合多源遥感数据,结合土壤呼吸地统计模型、趋势分析、地理探测器和残差分析等方法,系统开展了2001~2020年植被净生态系统生产力(NEP)时空变化特征分析及影响因素探究。结果表明:(1)在时间上,2001~2020年郴州市总体表现为碳汇,年均固碳总量达5.943×106 T Ca^(-1),NEP均值310.351 g Cm^(-2),平均增长速率2.066 g Cm^(-2)a^(-1)。(2)在空间上,NEP总体呈西高东低、北高南低分布,显著增加(P草地(318.650 g Cm^(-2))>耕地(257.509 g Cm^(-2))。(3)郴州市生态系统碳收支驱动机制复杂。NEP与气温、日照时数、相对湿度和高程等因子呈正相关,而与降水量和地表净辐射呈负相关,双因子对NEP空间分异性的交互作用明显高于单因子。(4)自然因子是郴州市植被NEP变化的主导因素(95.896%),但在城市边缘区等人类活动剧烈的区域,土地利用/覆被变化平均贡献率达59.761%。研究结果有助于准确把握区域陆地生态系统碳收支时空特征及其内在驱动机制,并为区域生态系统管理与可持续发展提供科学依据。

关键词：碳收支植被NEP 土地利用/覆被变化地理探测器土壤呼吸地统计模型

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基于有限单元法驾驶室防翻保护结构性能分析

机械设计与制造 2024年第2期396卷 231-235页

作者：王旭荣宋美玉彭伟利山东交通职业学院山东潍坊261206 山东大学山东济南250061

防滚翻保护装置(ROPS)设计的可靠性,直接影响驾驶员是否能得到有效的保护。根据实车尺寸和视野要求,进行ROPS结构设计和挠曲极限量DLV设计;利用UG建立驾驶室三维模型,并建立有限元分析模型;根据国家标准关于ROPS的规定,对驾驶室翻滚保... 详细信息

防滚翻保护装置(ROPS)设计的可靠性,直接影响驾驶员是否能得到有效的保护。根据实车尺寸和视野要求,进行ROPS结构设计和挠曲极限量DLV设计;利用UG建立驾驶室三维模型,并建立有限元分析模型;根据国家标准关于ROPS的规定,对驾驶室翻滚保护的安全性进行分析,重点对不同方向的承载进行分析;基于驾驶室翻滚测试试验台,选取侧向加载进行测试,验证模型分析的可靠性。结果可知:当侧向力增加到140kN时,驾驶室吸收的能量为16672J,满足最小能量吸收能力要求;侧向载荷加载时,应力和位移均出现最大值,分别为372MPa和11.86mm,满足材料承载和能量加载要求;试验获得侧向加载时的最大应力和最大位移,满足材料和设计尺寸的要求,且与仿真结果的误差均小于4%,表明仿真分析模型是可靠的。所设计的防翻保护装置可以起到保护作用,为此类设计生产提供参考。

关键词：驾驶室防翻保护装置有限单元法应力挠曲极限量

护理硕士专业学位研究生教育过程质量评价量表的编制

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护理研究 2022年第15期36卷 2655-2659页

作者：赵荣于文舟王瑛潍坊医学院山东261053 青岛大学附属医院

目的:编制护理硕士专业学位研究生教育过程质量评价量表,并进行信效度检验。方法:以学生发展理论为框架,通过文献研究、半结构化访谈、德尔菲专家函询形成初始量表。选取13个省份157名护理硕士专业学位研究生进行问卷调查。结果:共进行... 详细信息

目的:编制护理硕士专业学位研究生教育过程质量评价量表,并进行信效度检验。方法:以学生发展理论为框架,通过文献研究、半结构化访谈、德尔菲专家函询形成初始量表。选取13个省份157名护理硕士专业学位研究生进行问卷调查。结果:共进行了2轮专家函询,2轮函询专家积极系数分别为92.31%、100.00%,专家权威系数分别为0.817,0.825。第2轮专家协调系数为0.456~0.537(均P1的公因子4个,总累计方差贡献率为67.106%。结论:编制的护理硕士专业学位研究生教育过程质量评价量表具有较高的信效度,可作为评估护理硕士专业学位研究生教育过程质量的可靠工具。

关键词：护理硕士专业学位护理教育质量评价量表编制信度效度

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中国生理学会内分泌代谢、比较生理与应激生理学术会议

作者： Ping Luo Meimei Wu Po Gao Ting Gao Li Dong Xiaowei Ding Youqiang Meng Jiahong Qian Guohua Zhang weifang rong Hongqiao International Institute of Medical Research Tongren Hospital and Department of PhysiologyFaculty of Basic Medical SciencesShanghai Jiaotong University School of Medicine Key Laboratory of Cerebrocranial Diseases Ningxia Medical University

Numerous studies have demonstrated that estrogens may exert multifaceted effects on the cardiovascular system via activating the classical nuclear receptors ERa or ER|3 and the novel G protein coupled

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