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Science China(Life Sciences) 2024年第4期67卷 843-846页

作者： Cheng Guo weidong ye Danying Cao Mijuan Shi Wanting Zhang Yingyin Cheng Yaping Wang Xiao-Qin Xia Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijingChina The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Dear Editor,The expression profile demonstrates how each gene was expressed in the target tissue or cell at a given *** heterogeneity is a biological concept characterized by the presence of numerous cells with varying functions and expression profiles within organ/tissue *** is also cell-to-cell signaling,which implies that the extracellular environment and spatial location influence intracellular transcription,resulting in spatial heterogeneity of gene expression within the same cell type.

关键词： landscape fish database

采油单螺杆泵动态力学特性及疲劳寿命预测研究

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中国机械工程 2024年第8期35卷 1358-1365页

作者：祖海英孙金山叶卫东李大奇东北石油大学机械科学与工程学院大庆163318

针对常规采油螺杆泵定子橡胶疲劳失效的问题,以油田常用的GLB120-27型单螺杆泵为研究对象,开展了50℃油浸工况下定子橡胶材料疲劳试验,获取了橡胶材料的疲劳性能参数,并构建了疲劳寿命预测模型。运用ABAQUS软件对螺杆泵进行动态力学特... 详细信息

针对常规采油螺杆泵定子橡胶疲劳失效的问题,以油田常用的GLB120-27型单螺杆泵为研究对象,开展了50℃油浸工况下定子橡胶材料疲劳试验,获取了橡胶材料的疲劳性能参数,并构建了疲劳寿命预测模型。运用ABAQUS软件对螺杆泵进行动态力学特性有限元分析,基于数值模拟得到应力应变响应结果和定子橡胶材料单轴拉伸试验应力应变拟合表达式,计算了疲劳危险位置点应变能释放率的范围。利用构建的疲劳寿命预测模型对不同过盈量条件下的螺杆泵进行了疲劳寿命预测研究。结果表明,随着过盈量的增加,疲劳寿命呈指数函数下降,通过与某采油厂近5年单螺杆泵平均工作寿命的对比,发现基于裂纹扩展方法预测得到的螺杆泵定子橡胶疲劳寿命可以满足工程预测精度要求。

关键词：螺杆泵动态力学特性定子橡胶疲劳寿命预测

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螺旋缝隙泄漏双向流固耦合方法

排灌机械工程学报 2024年第6期42卷 556-562页

作者：叶卫东王波波祖海英李恒岩张恩来汪旭东北石油大学机械科学与工程学院黑龙江大庆163318

针对螺杆泵泄漏机理,利用数值模拟加数据拟合方法,提出了一种针对螺杆泵螺旋微小缝隙泄漏的双向流固耦合模拟方法.利用此方法,对比了单向流固耦合和双向流固耦合方法在螺杆泵泄漏计算结果的偏差,计算结果表明,间隙越小偏差越大,间隙为0.... 详细信息

针对螺杆泵泄漏机理,利用数值模拟加数据拟合方法,提出了一种针对螺杆泵螺旋微小缝隙泄漏的双向流固耦合模拟方法.利用此方法,对比了单向流固耦合和双向流固耦合方法在螺杆泵泄漏计算结果的偏差,计算结果表明,间隙越小偏差越大,间隙为0.500 mm时两结果相差20.3%,说明螺杆泵这种刚柔配合螺旋缝隙泄漏必须利用双向流固耦合求解才能获得准确结果.随后利用双向流固耦合方法对三维螺杆泵模型进行有限元分析求解,研究了初始间隙、流体介质黏度、螺杆泵单级腔室压差、进出口压力对螺杆泵泄漏的影响.结果显示:泄漏量随初始间隙的增大而增大,随流体介质黏度的增大而减少;进出口压力变化对螺杆泵泄漏影响很小,可以忽略;腔室间的压差造成橡胶定子变形形成间隙,间隙随着压差增大呈线性增大.最后利用双向流固耦合方法计算了不同举升压力螺杆泵的容积效率,与试验结果对比误差在±5%以内.

关键词：螺旋缝隙泄漏双向流固耦合容积效率螺杆泵

基于通阳理论的叶桂肿胀用药配伍规律数据挖掘

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世界中医药 2024年第7期19卷 1026-1030,1037页

作者：程悦方向明袁亚美叶卫东刘家鑫查君君安徽中医药大学合肥230000

目的:基于数据挖掘技术,探究叶桂治疗肿胀的用药配伍规律。方法:收集和整理《临证指南医案》肿胀篇医案及方药,用office 2010建立数据库,进行频数分析,应用IBM SPSS Modeler 18.0的Apriori算法进行关联规则分析,采用SPSS Statistics 26.... 详细信息

目的:基于数据挖掘技术,探究叶桂治疗肿胀的用药配伍规律。方法:收集和整理《临证指南医案》肿胀篇医案及方药,用office 2010建立数据库,进行频数分析,应用IBM SPSS Modeler 18.0的Apriori算法进行关联规则分析,采用SPSS Statistics 26.0进行因子分析。结果:共纳入83例处方,涉及药物117味,用药频数共计527次,高频药(频次≧10次)共15味,其中最高频单味药为茯苓、厚朴、生白术,对药以桂枝-甘草的使用频次最高,药物类别以化痰药、温里药、利水渗湿药为主;药性以温性为主,寒热并调;药味以辛、苦为主;归经以归脾、胃、肺经者最多;聚类分析中将15味高频中药聚为7类时效果最佳;关联规则分析出2组最常用药对;因子分析共提取出6个公因子。结论:叶桂治疗肿胀以通阳利水为要法,善用茯桂剂以奏温中通阳,化气利水之功,重脾肺、倡温补、善通利,务在宣肺、健脾、温肾,扶阳助气,上下并调,使阳气通达全身,通条水利,恢复人体气、血、津、液的正常功能,以达营卫调和、肿胀自除之效。

关键词：叶桂《临证指南医案》肿胀通阳理论用药规律聚类分析关联规则因子分析

射干麻黄汤及其辛、苦味药组拆方对寒哮大鼠气道炎症及肺组织TRPV1/TAS2R14表达的影响

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中国实验方剂学杂志 2024年第21期30卷 1-9页

作者：袁亚美叶卫东程悦李秋慧刘家鑫乔佳乐王坤方向明安徽中医药大学合肥230012 中医药防治肺系重大疾病应用转化安徽省重点实验室合肥230031 安徽中医药大学第二附属医院合肥230031 新安医学教育部重点实验室合肥230038

目的:探讨射干麻黄汤辛、苦味药组对哮喘寒哮证大鼠肺组织中瞬时受体电位通道香草醛亚型1(TRPV1)、苦味受体14(TAS2R14)蛋白表达的影响。方法:70只SD大鼠随机分为正常组、模型组、射干麻黄汤组、辛味药组、苦味药组、地塞米松组、桂龙... 详细信息

目的:探讨射干麻黄汤辛、苦味药组对哮喘寒哮证大鼠肺组织中瞬时受体电位通道香草醛亚型1(TRPV1)、苦味受体14(TAS2R14)蛋白表达的影响。方法:70只SD大鼠随机分为正常组、模型组、射干麻黄汤组、辛味药组、苦味药组、地塞米松组、桂龙咳喘宁组,10%卵清蛋白(OVA)结合氢氧化铝腹腔及四肢皮下注射致敏,联合2%OVA雾化及寒冷(2~4℃)刺激,激发大鼠寒哮模型。射干麻黄汤、辛味药、苦味药(6.43 g·kg^(-1))、地塞米松(0.5 g·kg^(-1))、桂龙咳喘宁组(10 g·kg^(-1))灌胃及雾化,空白组、模型组以等量生理盐水灌胃及雾化,持续干预3周。末次激发后,对大鼠肺组织进行苏木素-伊红(HE)、碘酸雪夫(PAS)、马松(Masson)染色观察气道炎症反应和细胞增殖情况。采用酶联免疫吸附测定法(ELISA)检测血清中白细胞介素-5(IL-5)、肿瘤坏死因子-α(TNF-α)、胸腺基质淋巴细胞生成素(TSLP)、转化生长因子-β_(1)(TGF-β_(1))含量;免疫荧光法检测TRPV1、TAS2R14的表达;蛋白免疫印迹法(Western blot)检测大鼠肺组织TRPV1、TAS2R14、磷脂酶Cβ(2 PLCβ_(2))、B细胞淋巴瘤-2(Bcl-2)、α-平滑肌肌动蛋白(α-SMA)表达。结果:与空白组比较,模型组大鼠饮水、饮食及体质量下降,气道炎性细胞浸润、杯状细胞增生、组织纤维化及胶原蛋白沉积情况增多,血清IL-5、TNF-α、TSLP和TGF-β_(1)含量升高(P<0.01),TRPV1、PLCβ_(2)、α-SMA的表达增加、TAS2R14、Bcl-2的表达降低(P<0.05,P<0.01);与模型组比较,射干麻黄汤、辛味药及苦味药物组大鼠饮水、饮食及体质量改善,气道炎性细胞浸润、杯状细胞增生、组织纤维化改变及胶原蛋白沉积情况减少;血清中IL-5、TNF-α、TSLP和TGF-β_(1)含量降低(P<0.01),TRPV1、PLCβ_(2)、α-SMA的表达减少,TAS2R14、Bcl-2表达增加(P<0.05,P<0.01)。结论:射干麻黄汤及其辛、苦味药物组可以明显减轻OVA诱发的哮喘大鼠气道炎症,降低TRPV1、PLCβ_(2)、α-SMA蛋白表达,激活TAS2R14、Bcl-2蛋白表达,其中苦味药组较辛味药组佳,辛苦相合其疗效增强,达到配伍之效。提示射干麻黄汤及其辛、苦味药物组能够减轻OVA诱发的哮喘气道炎症可能与抑制辛味相关TRPV1蛋白及激活苦味相关TAS2R14蛋白相关。

关键词：辛味药苦味药寒哮瞬时受体电位通道香草醛亚型1(TRPV1) 苦味受体14(TAS2R14)

“五位一体”总体布局推进有害生物综合治理理论创新

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农业技术与装备 2024年第9期 111-113,116页

作者：叶卫东李芬周祥吴少英邹游兴海南大学热带农林学院海南海口570228

“五位一体”总体布局是中国模式国家治理理论的重要组成部分,以“五位一体”总体布局思想为指导,应用M_5IP■Ec■S■P■(E∪C)通式,类比分析有害生物综合治理,由此获得推动植物保护专业治理总体布局创新发展的新思路,从而提出有害生物... 详细信息

“五位一体”总体布局是中国模式国家治理理论的重要组成部分,以“五位一体”总体布局思想为指导,应用M_5IP■Ec■S■P■(E∪C)通式,类比分析有害生物综合治理,由此获得推动植物保护专业治理总体布局创新发展的新思路,从而提出有害生物综合治理的“五位一体”新概念,以推动中国特色的现代化国家治理理论在植物保护专业中落实。

关键词： “五位一体”总体布局有害生物综合治理植物保护

Fish SCT: a zebrafish-centric database for exploration and visualization of fish single-cell transcriptome

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中国科学:生命科学英文版 2023年第9期66卷 2185-2188页

作者： Cheng Guo weidong ye Mijuan Shi You Duan Wanting Zhang Yingyin Cheng Xiao-Qin Xia Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China The Joint Laboratory for Lung Development and Related Diseases of West China Second University Hospital Sichuan University and School of Life Sciences of Fudan UniversityWest China Institute of Women and Children’s HealthWest China Second University HospitalSichuan UniversityChengdu 610041China Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University) Ministry of EducationChengdu 610041China NHC Key Laboratory of Chronobiology(Sichuan University) Chengdu 610000China

Dear Editor,Using sc RNA-seq technology, researchers may distinguish between different cell types and tissue heterogeneity as well as perform transcriptome investigations at the single-cell level (Regev et al., 2017). A significant amount of single-cell transcriptome data has been produced as a result of the technology’s quick development and widespread *** on other model species has also gathered a sizable quantity of transcriptome data at the single-cell level.

关键词： transcriptome fish

A 3D-printed microhemispherical shell resonator with electrostatic tuning for a Coriolis vibratory gyroscope

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Microsystems & Nanoengineering 2024年第2期10卷 25-35页

作者： Baoyin Hou ye Zhu Chaofan He weidong Wang Zhi Ding Wen He Yong He Lufeng Che College of Information Science and Electronic Engineering Zhejiang UniversityHangzhou310027China Center for Microelectronics Shaoxing InstituteZhejiang UniversityShaoxing312035China State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou310058China School of Mechanical Engineering Zhejiang UniversityHangzhou310058China

The emergence of microhemispherical resonant gyroscopes,which integrate the advantages of exceptional stability and long lifetime with miniaturization,has afforded new possibilities for the development of whole-angle ***,existing methods used for manufacturing microhemispherical resonant gyroscopes based on MEMS technology face the primary drawback of intricate and costly ***,we report the design,fabrication,and characterization of the first 3D-printable microhemispherical shell resonator for a Coriolis vibrating *** remarkably achieve fabrication in just two steps bypassing the dozen or so steps required in traditional *** utilizing the intricate shaping capability and ultrahigh precision offered by projection microstereolithography,we fabricate 3D high-aspect-ratio resonant structures and controllable capacitive air gaps,both of which are extremely difficult to obtain via MEMS *** addition,the resonance frequency of the fabricated resonators can be tuned by electrostatic forces,and the fabricated resonators exhibit a higher quality factor in air than do typical MEMS microhemispherical *** work demonstrates the feasibility of rapidly batch-manufacturing microhemispherical shell resonators,paving the way for the development of microhemispherical resonator gyroscopes for portable inertial ***,this particular design concept could be further applied to increase uptake of resonator tools in the MEMS community.

关键词： resonator tuning electrostatic

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丁甘仁辨治哮喘思路探析

江苏中医药 2024年第7期56卷 58-61页

作者：丁鹤影方文凯方向明程悦袁亚美叶卫东安徽中医药大学中医学院安徽合肥230012 安徽中医药大学第一临床医学院安徽合肥230012 安徽中医药大学护理学院安徽合肥230012

丁甘仁为孟河四大家的杰出代表,著名的中医临床家、教育家,其著录的《丁甘仁医案》一书,充分反映了丁甘仁诊疗临床各科之经验。《丁甘仁医案》卷四记录14例哮喘医案,可归纳丁甘仁对哮喘的辨治思路:治疗兼顾内外,疏外邪、化内饮以止咳喘... 详细信息

丁甘仁为孟河四大家的杰出代表,著名的中医临床家、教育家,其著录的《丁甘仁医案》一书,充分反映了丁甘仁诊疗临床各科之经验。《丁甘仁医案》卷四记录14例哮喘医案,可归纳丁甘仁对哮喘的辨治思路:治疗兼顾内外,疏外邪、化内饮以止咳喘,注重调理脏腑气机,重纳肾气、健脾气、降肺气以止咳喘;用药上多选温药化痰,以脾肺肾经药为主,善用鹅管石治喘急,海浮石化老痰,半夏配茯苓杜生痰之源,补骨脂配五味子治肾虚不纳,远志利肺气纳肾气;组方上采撷众方,汤丸并用。

关键词：丁甘仁《丁甘仁医案》哮喘

Preparation and characterization of SO_(4)^(2−)−/TiO_(2)and S_(2)O_(8)^(2)−/TiO_(2) catalysts

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Frontiers of Chemical Science and Engineering 2007年第1期1卷 45-49页

作者： MA Xuedan GUO Daishi JIANG Qizhong MA Zifeng MA Zhengfei ye weidong LI Chunbo Department of Chemical Engineering Shanghai Jiaotong UniversityShanghai 200240China College of Chemistry and Chemical Engineering Nanjing University of TechnologyNanjing 210009China Xinchang Pharmaceutical Factory Zhejiang Pharmaceutics Co.Ltd.Xinchang 312500China

Nanosized solid superacids SO_(4)^(2−)/TiO_(2)and S_(2)O_(8)^(2)−/TiO_(2),as well as MCM-41-supported SO_(4)^(2−)/ZrO_(2),were *** structures,acidities,and catalytic activities were investigated and compared using XRD,N2 adsorption-desorption,and in situ FTIR-pyridine adsorption,as well as an evaluation reaction with pseudoionone *** results showed that SO_(4)^(2−)/TiO_(2)and S_(2)O_(8)^(2)−/TiO_(2)possess not only nanosized particles with diameters<7.0 nm,a BET sur-face greater than 140 cm2/g and relatively regular mesostruc-tures with pores around 4.0 nm,but also a pure anatase phase and strong *** from the Lewis acid nature of SO_(4)^(2−)/ZrO_(2)/MCM-41,SO_(4)^(2−)/TiO_(2)and S_(2)O_(8)^(2)−/TiO_(2)exhibit mainly Brønsted *** strongest Brønsted acid sites were produced on SO_(4)^(2−)/TiO_(2)promoted with H2SO4,while Lewis acid sites on S_(2)O_(8)^(2)−/TiO_(2)even stronger than those on SO_(4)^(2−)/ZrO_(2)/MCM-41 were generated when persulfate solu-tion was used as sulfating *** of their distinct acid natures,SO_(4)^(2−)/TiO_(2)and S_(2)O_(8)^(2)−/TiO_(2)exhibited catalytic activities for the cyclization of pseudoionone that were much higher than that of SO_(4)^(2−)/ZrO_(2)/*** can be concluded that the existence of more Brønsted acid sites was favorable for proton participation in the cyclization reaction.

关键词： solid superacid S(4)^(2−)−/TiO_(2) S_(2)O_(8)^(2)−/TiO_(2) ionone cyclization