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Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1638-1656页

作者： Piao Zhao Lin Feng weidan jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang Haifeng Mi Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130SichuanChina Tongwei Co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610041SichuanChina

Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food *** developmental toxicity of OTA and the potential relieving strategy of natural products remain *** study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in *** A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the control group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur *** treatment consisted of 3 replicates(180 fish)for 60 *** Firstly,we cultured,purified,and identified myoblasts using the tissue block culture *** preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best ***,Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass ***,Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling ***,Cur could downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling *** In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in *** study confirms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

关键词： Curcumin Grass carp Myotoxic Ochratoxin A Protein deposition

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Mannan oligosaccharides alleviate oxidative injury in the head kidney and spleen in grass carp(Ctenopharyngodon idella)via the Nrf2 signaling pathway after Aeromonas hydrophila infection

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Journal of Animal Science and Biotechnology 2023年第4期14卷 1744-1758页

作者： Zhiyuan Lu Lin Feng weidan jiang Pei Wu Yang Liu Jun jiang Shengyao Kuang Ling Tang Shuwei Li Chengbo Zhong Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Sichuan Agricultural UniversityChengdu 611130China Sichuan Animal Science Academy Sichuan Animtech Feed Co.LtdChengdu 610066China Animal Breeding and Genetics Key Laboratory of Sichuan Province Animal Nutrition InstituteSichuan Academy of Animal ScienceChengdu 610066China

Background Mannan oligosaccharides(MOS)are recommended as aquaculture additives owing to their excellent antioxidant *** the present study,we examined the effects of dietary MOS on the head kidney and spleen of grass carp(Ctenopharyngodon idella)with Aeromonas hydrophila *** A total of 540 grass carp were used for the *** were administered six gradient dosages of the MOS diet(0,200,400,600,800,and 1,000 mg/kg)for 60 ***,we performed a 14-day Aeromonas hydrophila challenge *** antioxidant capacity of the head kidney and spleen were examined using spectrophotometry,DNA fragmentation,qRT-PCR,and Western *** After infection with Aeromonas hydrophila,400-600 mg/kg MOS supplementation decreased the levels of reactive oxygen species,protein carbonyl,and malonaldehyde and increased the levels of anti-superoxide anion,antihydroxyl radical,and glutathione in the head kidney and spleen of grass *** activities of copper-zinc superoxide dismutase,manganese superoxide dismutase,catalase,glutathione S-transferase,glutathione reductase,and glutathione peroxidase were also enhanced by supplementation with 400-600 mg/kg ***,the expression of most antioxidant enzymes and their corresponding genes increased significantly with supplementation of 200-800 mg/kg *** and protein levels of nuclear factor erythroid 2-related factor 2 also increased following supplementation with 400-600 mg/kg *** addition,supplementation with 400-600 mg/kg MOS reduced excessive apoptosis by inhibiting the death receptor pathway and mitochondrial pathway *** Based on the quadratic regression analysis of the above biomarkers(reactive oxygen species,malondialdehyde,and protein carbonyl)of oxidative damage in the head kidney and spleen of on-growing grass carp,the recommended MOS supplementation is 575.21,557.58,531.86,597.35,570.16,and 553.80 mg/kg,***,MOS supplementation could allev

关键词： Antioxidant Apoptosis Functional organs Grass carp Mannan oligosaccharides

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Dietary protein levels changed the hardness of muscle by acting on muscle fiber growth and the metabolism of collagen in sub-adult grass carp(Ctenopharyngodon idella)

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Journal of Animal Science and Biotechnology 2023年第2期14卷 729-744页

作者： Min Dong Lu Zhang Pei Wu Lin Feng weidan jiang Yang Liu Shengyao Kuang Shuwei Li Haifeng Mi Ling Tang Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceSichuan 611130China Healthy Aquaculture Key Laboratory of Sichuan Province Tongwei Co.Ltd.Chengdu ChinaSichuan 610041China Animal Nutrition Institute Sichuan Academy of Animal ScienceSichuan Animtech Feed Co.LtdChengdu 610066China.

Background:Nutrient regulation has been proven to be an effective way to improve the flesh quality in *** a necessary nutrient for fish growth,protein accounts for the highest proportion in the fish diet and is *** our team found that the effect of protein on the muscle hardness of grass carp was probably related to an increased collagen content,the mechanism for this effect has not been deeply ***,few studies have explored the protein requirements of sub-adult grass crap(Ctenopharyngodon idella).Therefore,the effects of different dietary protein levels on the growth performance,nutritional value,muscle hardness,muscle fiber growth,collagen metabolism and related molecule expression in grass carp were ***:A total of 450 healthy grass carp(721.16±1.98 g)were selected and assigned randomly to six experimen-tal groups with three replicates each(n=25/replicate),and were fed six diets with 15.91%,19.39%,22.10%,25.59%,28.53%and 31.42%protein for 60 ***:Appropriate levels of dietary protein increased the feed intake,percentage weight gain,specific growth rate,body composition,unsaturated fatty acid content in muscle,partial free amino acid content in muscle,and muscle hardness of grass *** protein levels also increased the muscle fiber density,the frequency of new muscle fibers,the contents of collagen and IGF-1,and the enzyme activities of prolyl 4-hydroxylases and lysyloxidase,and decreased the activity of matrix *** the molecular level,the optimal dietary protein increased col-lagen type Iα1(Colα1),Colα2,PI3K,Akt,S6K1,La ribonucleoprotein domain family member 6a(LARP6a),TGF-β1,Smad2,Smad4,Smad3,tissue inhibitor of metalloproteinase-2,MyoD,Myf5,MyoG and MyHC relative mRNA *** levels of the myostatin-1 and myostatin-2 genes were downregulated,and the protein expression levels of p-Smad2,Smad2,Smad4,p-Akt,Akt,LARP6 and Smad3 were ***:The appropriate levels of dietary

关键词： Collagen degradation Collagen synthesis Grass carp Muscle fibers Muscle hardness Protein

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Implications of vitamin D for flesh quality of grass carp(Ctenopharyngodon idella):antioxidant ability,nutritional value,sensory quality,and myofiber characteristics

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2596-2613页

作者： Yao Zhang Chaonan Li Xiaoqiu Zhou weidan jiang Pei Wu Yang Liu Hongmei Ren Lu Zhang Haifeng Mi Jiayong Tang Ruinan Zhang Lin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China. Key Laboratory for Animal Disease‑Resistant Nutrition of China Ministry of Education Sichuan Agricultural UniversityChengdu 611130China. Healthy Aquaculture Key Laboratory of Sichuan Province Tongwei Co.Ltd.Chengdu 610041SichuanChina.

Background Muscle represents a unique and complex system with many components and comprises the major edible part of *** D is a critical nutrient for animals and is known to enhance calcium absorption and immune *** recent years,dietary vitamin D supplementation in livestock has received increased attention due to biological responses including improving shear force in mammalian ***,the vitamin D acquisition and myofiber development processes in fish differ from those in mammals,and the effect of vitamin D on fish flesh quality is poorly ***,the influence of dietary vitamin D on fillet quality,antioxidant ability,and myofiber development was examined in grass carp(Ctenopharyngodon idella).Methods A total of 540 healthy grass carp,with an initial average body weight of 257.24±0.63 g,were allotted in 6 experimental groups with 3 replicates each,and respectively fed corresponding diets with 15.2,364.3,782.5,1,167.9,1,573.8,and 1,980.1 IU/kg vitamin D for 70 *** Supplementation with 1,167.9 IU/kg vitamin D significantly improved nutritional value and sensory quality of fillets,enhancing crude protein,free amino acid,lipid,and collagen contents;maintaining an ideal pH;and reduc-ing lactate content,shear force,and cooking loss relative to respective values in the control(15.2 IU/kg)*** myofiber diameter and the frequency of myofibers>50μm in diameter increased under supplementation with 782.5–1,167.9 IU/kg vitamin *** of oxidative damage biomarkers decreased,and the expression of antioxi-dant enzymes and nuclear factor erythroid 2-related factor 2 signaling molecules was upregulated in the 1,167.9 IU/kg vitamin D treatment compared to respective values in the control ***,vitamin D supplementation activated cell differentiation by enhancing the expression of myogenic regulatory factors and myocyte enhancer factors compared to that in the control *** addition,supplementation with 1,167.9 IU/kg

关键词： Antioxidant Grass carp Myofiber development Nutritional value Sensory quality Vitamin D

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An emerging role of arecoline on growth performance,intestinal digestion and absorption capacities and intestinal structural integrity of adult grass carp(Ctenopharyngodon idella)

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Animal Nutrition 2023年第4期15卷 173-186页

作者： Na Yao Lin Feng weidan jiang Pei Wu Hongmei Ren Hequn Shi Ling Tang Shuwei Li Caimei Wu Hua Li Yang Liu Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu611130SichuanChina Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceSichuan611130China Guangzhou Cohoo Biotech Co. Ltd.Guangzhou510663China Animal Nutrition Institute Sichuan Academy of Animal ScienceSichuan Animtech Feed Co.LtdChengdu610066SichuanChina

Arecoline is an alkaloid with important pharmacological effects in the plant areca nut,which has been demonstrated to be an agonist of muscarinic receptors(M receptor).This study explored the influences of dietary arecoline on growth performance,intestinal digestion and absorption abilities,antioxidant capacity,and the apical junction complex(AJC)of adult grass carp(Ctenopharyngodon idella).Adult grass carp(608to 1512 g)were fed at 6 graded levels of dietary arecoline(0,0.5,1.0,1.5,2.0,and 2.5 mg/kg diet)for *** results suggested that appropriate dietary supplementation of arecoline(1.0 mg/kg)increased growth parameters and intestinal growth in adult grass carp(P<0.05),enhanced digestion and absorption capacities(P<0.05),up-regulated muscarinic receptor 3(M3)mRNA level(P<0.05),increased the content of neuropeptide fish substance P(P<0.05),improved antioxidant capacity by activating the Keap1a/Nrf2signaling pathway(P<0.05),reduced intestinal mucosal permeability(P<0.05),and increased m RNA levels of tight junction(TJ)and adherent junction AJ-related proteins in fish by inhibiting the RhoA/ROCK signaling pathway(RhoA/ROCK/MLCK/NMII)(P<0.05).In addition,the appropriate arecoline supplementation for adult grass carp was determined to be 1.20,1.21,1.07,and 1.19 mg/kg based on percentage weight gain,lipase activity,serum diamine oxidase,and protein carbonyl,***,to the best of our knowledge,we investigated for the first time the effects and possible mechanisms of dietary arecoline on intestinal digestive and absorptive capacities and structural integrity in fish and evaluated the appropriate level of supplementation.

关键词： Arecoline Ctenopharyngodon idella Digestion capacity Absorption capacity Structural integrity Intestine

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Lysine deficiency impaired growth performance and immune response and aggravated inflammatory response of the skin,spleen and head kidney in grown-up grass carp (Ctenopharyngodon idella)

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Animal Nutrition 2021年第2期7卷 556-568页

作者： Yangyang Hu Lin Feng weidan jiang Pei Wu Yang Liu Shengyao Kuang Ling Tang Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengduChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan ProvinceSichuan Agricultural UniversityChengduChina Key Laboratory of Animal Disease-resistant Nutrition Sichuan ProvinceChina Key Laboratory of Animal Disease-resistant Nutrition Ministry of EducationChina Key Laboratory of Animal Disease-resistant Nutrition and Feed Ministry of Agriculture and Rural AffairsChina Animal Nutrition Institute Sichuan Academy of Animal ScienceChengduChina

This dissertation was primarily focused on the immune response,inflammatory response and molecular mechanisms in the skin,head kidney and spleen of grown-up grass carp(Ctenopharyngodon idella).Six iso-nitrogen diets differing in lysine concentrations(5.6,8.5,11.6,14.4,17.5 and 20.7 g/kg)were fed to 540 grass carp(164.85±0.79 g)for 60 *** that,grass carp were challenged by Aeromonas hydrophila for 6 *** study revealed that lysine deficiency(1)suppressed the growth performa,nce of the fish and decreased their ability to resist skin lesion morbidity,(2)impaired the immune organ s immune response by decreasing the gene expressions of mucin,liver-expressed antimicrobial peptide(LEAP)-2B,β-defensin-1 and LEAP-2A and the production of antibacterial compounds of grown-up grass carp,and(3)aggravated the inflammatory response of immune organs in the fish by increasing the gene expressions of pro-inflammatory cytokines(interferon y2[IFN-γ2],tumor necrosis factorα[TNF-α],interleukin[IL]-15,IL-17D,IL-12p40,IL-6 and IL-8)and down-regulating anti-inflammatory cytokines(IL-11,transforming growth factorβ1[TGF-β1],IL-10 and IL-4/13A),which were tightly correlated with signal transducer and activator of transcription(STAT)1 and STAT3 signaling pathway,*** different phenomenon in the skin,spleen and head kidney of fish may be correlated with the difference in gene *** addition,using quadratic regression analysis of percent weight gain(PWG),skin lesion morbidity,and the lysozyme activities in the spleen and head kidney,the dietary lysine requirements for grown-up grass carp were estimated to be 13.58,13.51,14.56 and 14.18 g/kg,respectively.

关键词： Lysine deficiency Grass carp(Ctenopharyngodon idella) Immune function Head kidney Spleen Skin

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Growth,digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp Ctenopharyngodonidella fed graded levels of dietary threonine

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Journal of Animal Science and Biotechnology 2015年第4期6卷 424-434页

作者： Yang Hong weidan jiang Shengyao Kuang Kai Hu Ling Tang Yang Liu Jun jiang Yongan Zhang Xiaoqiu Zhou Lin Feng Animal Nutrition Institute Sichuan Agricultural University Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural University Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Sichuan Agricultural University Sichuan Academy of Animal Science Animal Nutrition Institute Chinese Academy of Sciences Institute of Hydrobiology

Background： This study was carried out to investigate effects of threonine levels on growth, digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp （ Ctenopharyngodonidella）. Results： Weight gain, specific growth rate, feed intake and feed efficiency were significantly improved by dietary threonine （P 〈 0.05）. Intestinal activities of trypsin, chymotrypsin, alpha-amylase, lipase, alkaline phosphatase, y-glutamyl transpeptidase and creatine kinase took the similar trends. Contents of malondialdehyde and protein carbonyl in intestine and hepatopancreas were significantly decreased by dietary optimal threonine supplementation （P 〈 0.05）. Anti-superoxide anion capacity, anti-hydroxyl radical capacity, glutathione content and activities of superoxide dismutase, catalase and glutathione-S-transferase in intestine and hepatopancreas were enhanced by dietary threonine （P 〈 0.05）. Conclusions： Dietary threonine could improve growth, enhance digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp. The dietary threonine requirement of sub-adult grass carp （441.9-1,013.4 g） based on weight gain was 11.6 g/kg diet or 41.5 g/kg of dietary protein by quadratic regression analysis.

关键词： Antioxidant status Grass carp Intestinal enzyme activity Threonine

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The relationship between dietary methionine and growth,digestion,absorption,and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp(Ctenopharyngodon idella)

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Journal of Animal Science and Biotechnology 2017年第4期8卷 970-983页

作者： Pei Wu Ling Tang weidan jiang Kai Hu Yang Liu Jun jiang Shengyao Kuang ling tang Wuneng Tang Yongan Zhang Xiaoqiu Zhou Lin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Sichuan Agricultural UniversityChengdu 611130China Animal Nutrition Institute Sichuan Academy of Animal ScienceChengdu 610066China Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China

Background: Methionine is an essential amino acid for fish. The present study was conducted to investigate the effects of dietary methionine on growth performance, digestive and absorptive ability, as well as antioxidant capacity in the intestine and hepatopancreas of sub-adult grass carp(Ctenopharyngodon idella).Results: Dietary methionine deficiency significantly decreased percentage weight gain(PWG), feed intake, feed efficiency and protein efficiency ratio, as well as activities of hepatopancreatic glutamate-oxaloacetate transaminase and muscle glutamate-pyruvate transaminase in sub-adult grass carp(P < 0.05). Furthermore, methionine deficiency significantly reduced activities of trypsin, lipase and amylase in the intestine, Na^+/K^+-ATPase, alkaline phosphatase and γ-glutamyl transpeptidase in three intestinal segments, and creatine kinase(CK) in the proximal intestine(P < 0.05). However, an unexplained and significant increase in CK activity in the mid intestine was associated with dietary methionine deficiency. Malondialdehyde and protein carbonyl contents in the intestine and hepatopancreas were significantly increased by methionine deficiency(P < 0.05), whereas anti-hydroxyl radical capacity in the hepatopancreas and intestine, and anti-superoxide anion capacity in the intestine, were significantly decreased by methionine deficiency(P < 0.05). Moreover, methionine deficiency significantly decreased superoxide dismutase and glutathione reductase activities, glutathione contents in the hepatopancreas and intestine, as well as glutathione peroxidase activity in the intestine(P < 0.05), whereas it significantly increased activities of catalase in the hepatopancreas and glutathione-S-transferase in the hepatopancreas and intestine(P < 0.05).(Continued on next page)(Continued from previous page)Conclusions: The present results demonstrated that dietary methionine deficiency induced poor growth, and decreased digestive and absorptive function and antioxidant capac

关键词： Antioxidant status Ctenopharyngodon idella Digestive and absorptive capacities Methionine

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Growth rate,body composition, digestive enzymes and transaminase activities,and plasma ammonia concentration of different weight Jian carp(Cyprinus carpio var. Jian)

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Animal Nutrition 2015年第4期1卷 373-377页

作者： Jun jiang Lin Feng Ling Tang Yang Liu weidan jiang Xiaoqiu Zhou College of Animal Science and Technology Sichuan Agricultural University Animal Nutrition Institute Sichuan Agricultural University Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural University Animal Nutrition Institute Sichuan Academy of Animal Science

The present study investigated the effect of body weight on body composition, digestive and absorptive capacity, transaminase activities in hepatopancreas and muscle, and plasma ammonia concentration of Jian carp(Cyprinus carpio ***). A total of 750 Jian carps(18.0 ± 0.2 g) were randomly distributed into five groups with three replicates and fed the same diet for 56 days. Tissue and plasma samples were collected on days 14, 28,42, and 56. The results were used to develop a mathematical model for specific growth rate, body moisture and fat content, aspartate transaminase activity and alanine aminotransferase activity in hepatopancreas and muscle, plasma ammonia concentration, and trypsin, chymotrypsin, lipase, and amylase activities in hepatopancreas and intestine, activities of creatine kinase, Na^+/K^+-ATPase, alkaline phosphatase, and γ-glutamyl transpeptidase in intestine in Jian carp. There were linear relationships between natural logarithms of above indexes and body weight. The body moisture and fat content, digestive and absorptive enzymes activities, and transaminase activities showed negative allometry against body weight of Jian carp which were partial reasons to explain fish growth rate decreasing.

关键词： Allometry Body weight Digestive enzymes Jian carp Plasma ammonia concentration Transaminase

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Dietary isoleucine improved flesh quality,muscle antioxidant capacity,and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂)

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Journal of Animal Science and Biotechnology 2021年第4期12卷 1384-1398页

作者： Qin jiang Mingyao Yan Ye Zhao Xiaoqiu Zhou Long Yin Lin Feng Yang Liu weidan jiang Pei Wu Yan Wang Defang Chen Shiyong Yang Xiaoli Huang Jun jiang College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130China Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityYa’an 625014China

Background:Muscle is the complex and heterogeneous tissue,which comprises the primary edible part of the trunk of fish and *** studies have shown that dietary isoleucine(Ile)exerts beneficial effects on growth in aquatic ***,there were limited studies regarding the benefits of Ile on fish muscle and their effects on flesh quality and muscle ***,this study was conducted to explore whether dietary Ile had affected flesh quality and muscle growth in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂).Methods:A total of 630 hybrid fish,with an initial average body weight of 33.11±0.09 g,were randomly allotted into seven experimental groups with three replicates each,and respectively fed seven diets with 5.0,7.5,10.0,12.5,15.0,17.5,and 20.0 g Ile/kg diets for 8 ***:In the present study,we demonstrated that Ile significantly:(1)increased muscle protein and lipid contents and the frequency distribution of myofibers with≤20μm and≥50μm of diameter;(2)improved pH value,shear force,cathepsin B and L activities,hydroxyproline content,resilience,cohesiveness,and decreased cooking loss,lactate content,hardness,springiness,gumminess,and chewiness;(3)decreased reactive oxygen species(ROS),malondialdehyde(MDA),and protein carbonyl(PC)contents,GCLC and Keap1 mRNA levels,and up-regulated CuZnSOD,CAT,GPX1a,GST,and Nrf2 mRNA levels;(4)up-regulated the insulin-like growth factor 1,2(IGF-1,IGF-2),insulin-like growth factor 1 receptor(IGF-1R),proliferating cell nuclear antigen(PCNA),Myf5,Myod,Myog,Mrf4,and MyHC mRNA levels,and decreased MSTN mRNAlevel;(5)increased muscle protein deposition by activating AKT-TOR-S6K1 and AKT-FOXO3a signaling ***:These results revealed that dietary Ile improved flesh quality,which might be due to increasing nutritional content,physicochemical,texture parameters,and antioxidant ability;promoting muscle growth by affecting myocytes hyperplasia and hypertrophy,and muscle protein depos

关键词： Antioxidant capacity Flesh quality Hybrid bagrid catfish Isoleucine Myogenesis Protein deposition

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