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Food Science and Human Wellness 2024年第1期13卷 360-369页

作者： Xiangxin Li Yijing Pu Haitao jiang Wenxiao Jiao Wenjun Peng Wenli Tian weibo jiang Xiaoming Fang Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China College of Food Science and Nutritional Engineering China Agricultural UniversityBeijing 100083China College of Food Science and Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353China

This study was aimed to analyze the effect of procyanidin B2(PC)and tannin acid(TA)on the activities of cholesterol esterase(CEase)and the inhibitory mechanisms of enzymatic *** interaction mechanisms were investigated by enzymatic kinetics,multi-spectroscopy methods,thermodynamics analysis,molecular docking,and dynamic *** and TA could bind with CEase and inhibit the activity of enzyme in a mixed-competitive manner and non-competitive manner,which was verified by molecular docking simulations and dynamics ***,PC and TA showed the synergistic inhibition with ***,UVvis and the thermodynamic analysis revealed that the complexes were formed from CEase and inhibitors by noncovalent *** revealed by the circular dichroism results,both PC and TA decreased enzymatic activities by altering the conformations of *** inhibition of PC and TA on CEase might be one mechanism for its cholesterol-lowering effect.

关键词： Cholesterol esterase Polyphenols Interaction mechanisms Molecular dynamic simulation Synergistic effect

Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway

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Food Science and Human Wellness 2024年第2期13卷 909-919页

作者： Xiangxin Li Yijing Pu Bangdi Liu Xiaoming Fang Wenjun Peng weibo jiang Wenli Tian Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China College of Food Science and Nutritional Engineering China Agricultural UniversityBeijing 100083China Academy of Agricultural Planning and Engineering Ministry of Agriculture and Rural AffairsBeijing 100125China

Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease(NAFLD).It remains unknown whether dietary intake of condensed tannins(CTs)have distinguishable effects to alleviate liver damage caused by a high cholesterol *** C57BL/6 mice were fed a high cholesterol diet for 6 weeks,and given CTs treatment at a dosage of 200 mg/(kg·day)at the same *** results indicated that compared with mice fed a normal diet,a high cholesterol diet group resulted in significant weight loss,dysregulation of lipid metabolism in blood and liver,and oxidative stress in the liver,but CTs treatment dramatically reversed these negative *** and eosin(H&E)staining and frozen section observation manifested that CTs treatment could effectively reduce the deposition of liver cholesterol and tissue necrosis caused by high cholesterol *** alleviated liver injury mainly by regulating the expression of related genes in cholesterol metabolism pathway and AMPK *** results confirmed that CTs have remarkable cholesterol lowering and anti-liver injury effects in vivo.

关键词： Condensed tannins High cholesterol Liver injury Antioxidants Transcriptomic analysis

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关于晶体熔化相关问题的探讨与启示

物理教学 2024年第5期46卷 38-41页

作者：蒋炜波清华大学附属中学北京100084

从一道考查晶体熔化的命题出发,结合两套义务教育物理教材,将实验研究与理论分析相结合,指出命题的科学性问题,探讨关于晶体熔化的相关问题,提出对教学的建议。

关键词：晶体熔点晶态非晶态启示建议

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关于试电笔相关认识疑难的探讨

物理教学 2024年第3期46卷 40-42,46页

作者：王宏蒋炜波清华大学附属中学北京100084

试电笔是一种家庭电路检测工具,也是初中阶段学生比较难以理解的物理仪器。文章针对教学中教师和学生对试电笔的一些理解认识误区进行分析探讨,以期为教学提供借鉴。

关键词：试电笔物理探讨

关注课标理念凸显育人方法——第十五届全国中学物理青年教师教学大赛印象和感悟

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物理教学 2024年第7期46卷 2-6页

作者：蒋炜波赵坚清华大学附属中学北京100084 昆明市五华区基础教育发展研究院云南650031

以第十五届全国中学物理青年教师教学大赛的获奖课例为依据,结合课程改革理念和立德树人使命,分析参赛教师呈现出的教育教学特点,思考教师专业发展的空间方向,探讨对中学物理教育的启示与借鉴。

关键词：物理教学教学大赛启示借鉴

传统村落装配式民居推广策略研究——以贵州黔东南地区为例

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项目管理技术 2024年第2期22卷 5-11页

作者：刘承洋龚鑘蒋维波刘爽贵州大学建筑与城市规划学院贵州贵阳550001

随着乡村振兴战略的推进和绿色建筑的发展,装配式建筑在传统村落民居中的应用越来越受到重视。为此,首先,运用CiteSpace软件对推广装配式建筑的热点影响因素、发展趋势进行分析,构建出研究热点共现图谱;其次,结合SWOT法确定贵州黔东南... 详细信息

随着乡村振兴战略的推进和绿色建筑的发展,装配式建筑在传统村落民居中的应用越来越受到重视。为此,首先,运用CiteSpace软件对推广装配式建筑的热点影响因素、发展趋势进行分析,构建出研究热点共现图谱;其次,结合SWOT法确定贵州黔东南地区传统村落中推广装配式民居的16个内外部因素;最后,构建传统村落装配式民居推广战略选择模型,并提出相关建议。研究可为贵州黔东南地区传统村落乃至全国其他传统村落推广装配式民居提供参考。

关键词：装配式民居传统村落 CiteSpace SWOT分析推广策略

Aquafeed fermentation improves dietary nutritional quality and benefits feeding behavior,meat flavor,and intestinal microbiota of Chinese mitten crab(Eriocheir sinensis)

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Animal Nutrition 2023年第3期14卷 1-19页

作者： weibo jiang Xiaoyan Jia Ningjun Xie Chuang Wen Shuo Ma Guangzhen jiang Xiangfei Li Cheng Chi Dingdong Zhang Wenbin Liu Key Laboratory of Aquatic Nutrition and Feed Science of jiangsu Province College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

Normally,proper fermentation can be an efficient and widely used method to improve feed quality in animal rearing;however,the studies on crustaceans,especially Eriocheir sinensis,remain *** study aimed to investigate whether feed fermentation could meliorate dietary nutritional value and benefit *** ***,non-fermented feed(NFD)and fermented feed(FD)were produced and assessed,***,the“Y”maze feed choice behavior test(180 times;30 times,6 rounds)was conducted to assess the attractiveness of these 2 feeds for ***,a total of 80 crabs(44.10±0.80 g)were randomly assigned into 2 groups with 4 replicates,and fed the experimental diets for 8 weeks to evaluate the effects of each feed on growth,antioxidant capacity,meat flavor,and intestinal *** this study,FD showed higher levels of crude protein(P<0.01),soluble protein(P<0.01),amino acids(P<0.05),lactic acid(P<0.001),and lower levels of crude fiber(P<0.05)and antinutritional factors(agglutinin,trypsin inhibitor,glycinin,andβ-conglycinin)(P<0.001)than ***,FD was more attractive to crabs than NFD(P<0.01)and it stimulated the appetite of crabs more than NFD(P<0.05).The growth performance,feed efficiency,and digestive enzyme activity of FDfed crabs were significantly higher than those of NFD-fed crabs(P<0.05).The electronic sensory measurements and free amino acid profiles revealed that the FD diet had positive impacts on the meat flavor of crabs,particularly in“sweet”and“umami”***,the antioxidant capacity of FD-fed crabs was significantly higher than that of NFD-fed crabs(P<0.05).Fermented feed also affected the diversity and composition of intestinal *** functional prediction of microbial communities showed that crabs fed FD had a better microecological environment in the *** conclusion,the fermentation of aquafeed could be an effective approach to enhance feed quality and therefore benefit *** rearing.

关键词： Fermentation feed Feed attraction Meat flavor Intestinal microflora Nutritional composition Eriocheir sinensis

Changes in Extractable and Non-extractable Polyphenols and Their Antioxidant Properties during Fruit On-tree Ripening in Five Peach Cultivars

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Horticultural Plant Journal 2019年第4期5卷 137-144页

作者： Hui Liu weibo jiang Jiankang Cao Yucai Li College of Food Engineering Ludong UniversityYantaiShandong264025China College of Food Science and Nutritional Engineering China Agricultural UniversityBeijing100083China

To evaluate the changes of total phenolic content and antioxidant capacities of five peach cultivars, this work measured them separately in extractable polyphenols and non-extractable polyphenols during the last month of ripening. Total phenolic content was detected using the Folin-Ciocalteau reagent, while total antioxidant activity was detected by DPPH and FRAP assay. In addition, the contribution of extractable polyphenols and non-extractable polyphenols to the total antioxidant activity of peaches was investigated. It is noted that ripening caused a decrease of total phenolic content associated to extractable polyphenols and non-extractable polyphenols by 45.1%–74.6%, 20.7%–41.7%, respectively. Additionally, total antioxidant activity decreased over the ripening period with a high correlation with total phenolic content. Extractable polyphenols contributed more than 50% to the total antioxidant activity, while the non-extractable antioxidants contributed around 31.3%–45.7%(DPPH) or 12.6%–25.4%(FRAP), which suggests that the antioxidant properties of peaches may be undervalued in previous researches.

关键词： Peach Extractable phenolics Non-extractable phenolics Antioxidant activity Ripening

Identification of a senescence-related protease in coriander leaves

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Chinese Science Bulletin 2002年第13期47卷 1096-1099页

作者： weibo jiang Xiangjuan Zhou Yumei Zhao Pengcheng Liu China Agr Univ Sch Food Sci Beijing 100094 Peoples R China

Senescence-related protease may play an important role in leaf senescence. By improved SDS-Gela-tin-PAGE assay, a 63 ku senescence-related protease (63 SRP) in coriander leaves was identified. Activity of 63 SRP was increased in parallel to the advance of coriander leaf senescence, and inhibited by treating the leaf with gibberellic acid, and enhanced by ethylene treatment. The 63 SRP was suggested to be a serine protease based on the fact that its activity was inhibited by the protease inhibitor PMSF. The optimal temperature for the activity of the 70 ku protease was 50°C. The maximal activity was observed at pH 6-9, some activity could be observed on the gel slices incubated at pH 5 or 11. The 63 SRP was partly purified by the way of ammonium sulfate precipitation and then gel slicing after gel elec-trophoresis.

关键词： coriander leaf senescence protease protein degradation.

PsERF1B-PsMYB10.1-PsbHLH3 module enhances anthocyanin biosynthesis in the flesh-reddening of amber-fleshed plum (cv. Friar) fruit in response to cold storage

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Horticulture Research 2023年第6期10卷 276-286页

作者： Ranran Xu Yubei Wang Limin Wang Zhilei Zhao Jiankang Cao Daqi Fu weibo jiang College of Food Science and Nutritional Engineering China Agricultural UniversityBeijing 100083China School of Chemical Engineering&Food Science Zhengzhou University of TechnologyZhengzhou 450044China College of Quality and Technical Supervision Hebei UniversityBaoding 071002China

Flesh-reddening usually occurs in the amber-fleshed plum(Prunus salicina Lindl.)fruit during cold storage but not during ambient storage direct after *** is not clear how postharvest cold signal is mediated to regulate the anthocyanin biosynthesis in the forming of flesh-reddening *** this study,anthocyanins dramatically accumulated and ethylene produced in the‘Friar’plums during cold storage,in comparison with plums directly stored at ambient *** of genes associated with anthocyanin biosynthesis,as well as transcription factors of PsMYB10.1,PsbHLH3,and PsERF1B were strongly stimulated to upregulated in the plums in the period of cold *** of ethylene act with 1-methylcyclopropene greatly suppressed flesh-reddening and downregulated the expression of these *** overexpression and virus-induced gene silencing assays in plum flesh indicated that PsMYB10.1 encodes a positive regulator of anthocyanin *** transient overexpression of PsERF1B,coupled with PsMYB10.1 and PsbHLH3,could further prompt the anthocyanin biosynthesis in a tobacco leaf *** from yeast two-hybrid and luciferase complementation assays verified that PsERF1B directly interacted with ***1B and PsMYB10.1 enhanced the activity of the promoter of PsUFGT individually,and the enhancement was prompted by the co-action of PsERF1B and ***,the stimulation of the PsERF1B-PsMYB10.1-PsbHLH3 module mediated cold signal in the transcriptomic supervision of the anthocyanin biosynthesis in the‘Friar’*** results thereby revealed the underlying mechanism of the postharvest alteration of the flesh phenotype of‘Friar’plums subjected to low temperature.

关键词： temperature. prompt ambient