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Anticancer effect and apoptosis induction of gambogic acid in human gastric cancer line BGC-823

World Journal of Gastroenterology 2005年第24期11卷 3655-3659页

作者： weiliu Qing-LongGuo Qi-DongYou LiZhao Hong-YanGu Sheng-TaoYuan DepartmentofPhysiology ChinaPharmaceuticalUniversityNanjing210009JiangsuProvinceChina DepartmentofMedicinalChemistry ChinaPharmaceuticalUniversityNanjing210009JiangsuProvinceChina theNationalLaboratoryofNewDrugScreen ChinaPharmaceuticalUniversityNanjing210009JiangsuProvinceChina

AIM: To investigate the anticancer effect of a traditional Chinese medicine gambogic acid (GA) in human gastric cancer line BGC-823 and further study the mechanism of apoptosis induction of ***: Low differential human gastric cancer line BGC-823 were treated with GA at different doses and different times, the inhibitory rates were detected by MTT assay. Apoptosis induced by GA in BGC-823 cells was observed by Annexin-V/PI doubling staining flow cytometry assay. And T/C (%) was chosen to detect the inhibition of GA on human gastric adenocarcinoma BGC-823 nude mice xenografts. Apoptosis on nude mice xenografts was observed by Annexin-V/PI doubling staining flow cytometry assay and DNA fragmentation assay. To further determine the molecular mechanism of apoptosis induced by GA, the changes on the expression of bcl-2 and bax genes were detected by ***: After incubation with GA, low differential human gastric cancer line BGC-823 was dramatically inhibited in a dose-dependent manner. After these cells were exposedto GA for 24, 48 and 72 h, the IC50 value was 1.02±0.05, 1.41±0.20 and 1.14±0.19 μmol/L, respectively. Apoptosis in BGC-823 cells induced by GA was observed by AnnexinV/PI doubling staining flow cytometry assay. The apoptotic population of BGC-823 cells was about 12.96% and 24.58%, respectively, when cells were incubated with 1.2 μmol/L GA for 48 and 72 h. T/C (%) of human gastric carcinoma adenocarcinoma BGC-823 nude mice xenografts was 44.3, when the nude mice were treated with GA (8 mg/kg). Meanwhile, apoptosis induced by GA was observed in human gastric carcinoma adenocarcinoma BGC-823 nude mice xenografts. The increase of bax gene and the decrease of bc1-2 gene expressions were found by ***: The inhibition of GA on human gastric cancer line BGC-823 was confirmed. This effect connects with the inducing apoptosis in BGC-823 cells and the molecular mechanism might be related to the reduction of expression of apoptosis-regulated g

关键词： Gambogic acid Apoptosis bcl-2 bax

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Proteomics to display tissue repair opposing injury response to LPS-induced liver injury

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World Journal of Gastroenterology 2004年第18期10卷 2701-2705页

作者： Xiao-weiliu Fang-GenLu Guang-SenZhang Xiao-PingWu YuYou Chun-HuiOuyang Dong-YeYang DepartmentofGastroenterology theSecondXiangyaHospitalCentralSouthUniversityChangsha410011HunanProvinceChina DepartmentofHematology theSecondXiangyaHospitalCentralSouthUniversityChangsha410011HunanProvinceChina

AIM: To examine the protein expression alterations in liver injury/repair network regulation as a response to gut-derived lipopolysaccharide (LPS) treatment, in order to anticipate the possible signal molecules or biomarkers in signaling LPS-related liver ***: Male BALB/c mice were treated with intraperitoneal (i.p.) LPS (4 mg/kg) and sacrificed at 0, 6, 24 and 30 h to obtain livers. The livers were stained with hematoxylin and eosin for histopathologic analyses. Total liver protein was separated by two-dimensional gel electrophoresis (2-DE). The peptide mass of liver injury or repair related proteins were drawn up and the protein database was searched to identify the ***: Observations were as follows: (1) TRAIL-R2 was down regulated in livers of LPS-treated mice. TNFAIP1 was significantly up regulated at 6 h, then down- regulated at 24, 30 h with silent expression during senescent stage.(2) The amount of metaxin 2 and mitochondria import inner membrane translocase subunit TIM8a (TIMM8A) was increased upon treatment with LPS, (3) P34 cdc2 kinase was significantly up-regulated 30 h after LPS administration with silent expression during senescent, 6, 24 h treated stage. (4) The amount of proteasome activator 28 alpha subunit (PA28), magnesium dependent protein phosphatase(MDPP) and lysophospholipase 2 was decreased 6 h after LPS treatment but recovered or up-regulated 24 and 30 h after LPS ***: LPS-treated mouse liver displaying a timedependent liver injury can result in expression change of some liver injury or repair related proteins.

关键词：组织修复免疫反应反作用 LPS-感应肝损伤脂多糖

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Studies of Ce(Ⅲ)-ALC-F Interacting with Herring Sperm DNA by Electrochemical, Fluorimetric and UV-spectrophotometric Method

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Chinese Chemical Letters 2004年第4期15卷 443-446页

作者： GuangHanLU weiliu ChuanYinLIU LiPingJIANG FengSONG CollegeofChemistry CentralChinaNormalUniversityWuhan430079

Ce(Ⅲ)-ALC-F complex can react with hsDNA to form an electrochemically non-active supermolecular complex Ce(Ⅲ)-ALC-F-DNA in the buffer solution of (CH2)6N4(pH=4.9), which results in the decrease of the peak current of Ce(Ⅲ)-ALC-F. This method can be applied to determine DNA concentration. In addition, by using fluorimetric and UV-spectrophotometric methods with studies of denatured DNA and the effect of NaCl solution , it is also found that the binding mode is intercalation.

关键词： Ce(Ⅲ)-ALC-F electrochemically non-active supermolecular complex herring sperm DNA(hsDNA) fluorimetry UV-spectrophotometry intercalation.

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Electrolyte additive enhances the electrochemical performance of Cu for rechargeable Cu//Zn batteries

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Journal of Energy Chemistry 2023年第2期77卷 172-179,I0005页

作者： Xinxin Song Chenggang Wang Dongdong Wang Huili Peng Cheng Wang Chunsheng Wang weiliu Fan Jian Yang Yitai Qian Key Laboratory of Colloid and Interface Chemistry Ministry of EducationSchool of Chemistry and Chemical EngineeringShandong UniversityJinan 250100ShandongChina Hefei National Laboratory for Physical Science at Microscale Department of ChemistryUniversity of Science and Technology of ChinaHefei 230026AnhuiChina

Cu-based cathodes in aqueous batteries become very attractive in view of high theoretical capacity,moderate operation voltage and rich reserves of raw ***,their applications are obstructed by serious side *** side reaction mainly arises from the spontaneous formation of Cu_(2)O,which occupies the electrode surface and lowers the reaction ***,Na_(2)EDTA is introduced to address these *** experimental results and theoretical calculations indicate that the Na_(2)EDTA reshapes the solvation structure of Cu^(2+)and modifies the electrode/electrolyte ***,the redox potential of Cu^(2+)/Cu_(2)O is reduced and the surface of Cu is protected from H2O,thereby inhibiting the formation of Cu_(2)***,the change in the solvation structure reduces the electrostatic repulsion between Cu^(2+)and the cathode,leading to high local concentration and benefiting uniform *** results shed light on the applications of rechargeable Cu-based batteries.

关键词： Cu Zn Reaction mechanisms Electrolyte additive Rechargeable batteries

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COMBINED SURGICAL THERAPY FOR HEPATOLITHIASIS

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Chinese Medical Sciences Journal 2005年第2期20卷 123-125,页

作者： Xiao-dongHe weiliu Bing-luLi Zheng-huanZhang Jian-xiZhang DepartmentofGeneralSurgery PekingUnionMedicalCollegeHospitalChineseAcademyofMedicalSciences＆PekingUnionMedicalCollegeBeijing100730

Objective To evaluate the combined surgical therapy for calculi of intrahepatic duct. Methods One hundred and eight cases of hepatolithiasis treated in our hospital from January 1986 to September 2003 were summarized and analyzed retrospectively. The primary surgery included 57 cases of biliary tract exploration and cholangiolithotomy, 26 cases of cholangiojejunostomy, and 25 cases of partial hepatectomy. Of these cases, 156 operations were performed totally. There were 36 patients undergoing two or more operations for cholangiolithiasis problem. Fiberoptic choledochoscopy was employed to examine and remove the residual stones during and after operation in all cases. Results The residual stone rates were 57.89% (33/57), 26.92% (7/26), and 24.0% (6/25) for biliary tract exploration and cholangiolithotomy, cholangiojejunostomy, and partial hepatectomy, respectively. Fiberoptic choledochoscopy was utilized pre- and post-operatively for each patient, which was performed 2 to 9 times on each case. Following the combined treatment, the rate of residual stone after operation decreased to 12.96% (14/108). Conclusions The combined surgical therapy is valuable for resolving the problem of hepatolithiasis in most of the patients. Regarding the clinical outcomes of different surgical procedures, partial hepatectomy is superior to cholangiojejuno- stomy or biliary tract exploration and cholangiolithotomy. Fiberoptic choledochoscopy is also important to reduce the occurrence of residual stones and the rate of reoperation.

关键词： hepatolithiasis surgical treatment choledochoscopy

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Study on relationship between expression level and molecular conformations of gene drugs targeting to hepatoma cells in vitro

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World Journal of Gastroenterology 2003年第9期9卷 1954-1958页

作者： Dong-YeYang Fang-GenLu Xi-XiangTang Shui-PingZhao Chun-HuiOuyang Xiao-PingWu Xiao-weiliu Xiao-YingWu DepartmentofGastroenterology XiangyaSecondHospitalCentralSouthUniversityChangsha410011&InstituteofShengLifeGeneDrugsChangsha410003HunanChina DepartmentofCardiovascularology XiangyaSecondHospitalCentralSouthUniversityChangsha410011HunanChina DepartmentofElectronMicroscope XiangyaMedicalSchoolCentralSouthUniversityChangsha410011Hunan.China

AIM: To increase exogenous gene expression level bymodulating molecular conformations of targeting gene ***: The full length cDNAs of both P40 and P35subunits of human interleukin 12 were amplified throughpolymerase chain reaction (PCR) and cloned into eukaryoticexpressing vectors pcDNA3.1(±) to construct plasmids of P(+)/IL-12, P(+)/P40 and P(-)/P35. These plasmids werecombined with ASOR-PLL to form two targeting gene drugs[ASOR-PLL-P(+)/IL-12 and ASOR-PLL-P(+)/P40 + ASOR-PLL-P(-)/P35] in optimal ratios. The conformations of these twodrugs at various concentrations adjuvant were examined underelectron microscope (EM) and the drugs were transfected intoHepG2 (ASGr+) cells. Semi-quantitative reverse transcriptionpolymerase chain reaction (RT-PCR) was performed withtotal RNA extracted from the transfected cells to determinethe hiL12 mRNA transcript level. The hiL12 protein in thecultured supernatant was measured with enzyme-linkedimmunosorbent assay (ELISA) 48 hours after ***: Targeting gene drugs, whose structures weregranular and circle-like and diameters ranged from 25 nmto 150 nm, had the highest hIL-12 expression level. ThehIL-12 expression level in the group co-transfected withASOR-PLL-P(+)/P4o and ASOR-PLL-P(-)/P35 was higher thanthat of ASOR-PLL-P(+)/IL-12 transfected ***: The molecular conformations of targetinggene drugs play an important role in exogenous geneexpression level, the best structures are granular and circle-like and their diameters range from 25 nm to 150 nm. Thesizes and linking styles of exogenous genes also have someeffects on their expression level.

关键词：肝癌基因疗法分子构造酶联免疫吸附试验

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The Use of the Ratio between the Veno-arterial Carbon Dioxide Difference and the Arterial-venous Oxygen Difference to Guide Resuscitation in Cardiac Surgery Patients with Hyperlactatemia and Normal Central Venous Oxygen Saturation

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Chinese Medical Journal 2015年第10期128卷 1306-1313页

作者： WeiDu YunLong Xiao-TingWang Da-weiliu Department of Critical Care Medicine Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China

Background：After cardiac surgery,central venous oxygen saturation （ScvO2） and serum lactate concentration are often used to guide resuscitation;however,neither are completely reliable indicators of global tissue *** observational study aimed to establish whether the ratio between the veno-arterial carbon dioxide and the arterial-venous oxygen differences （P（v-a）CO2/C（a-v）O2） could predict whether patients would respond to resuscitation by increasing oxygen delivery （DO2）.Methods：We selected 72 patients from a cohort of 290 who had undergone cardiac surgery in our institution between January 2012 and August *** selected patients were managed postoperatively on the Intensive Care Unit,had a normal ScvO2,elevated serum lactate concentration,and responded to resuscitation by increasing DO2 by 〉10%.As a consequence,48 patients responded with an increase in oxygen consumption （VO2） while VO2 was static or fell in ***：At baseline and before resuscitative intervention in postoperative cardiac surgery patients,a P（v-a）CO2/C（a-v）O2 ratio ≥1.6 mmHg/ml predicted a positive VO2 response to an increase in DO2 of〉1 0% with a sensitivity of 68.8% and a specificity of 87.5%.Conclusions：P（v-a）CO2/C（a-v）O2 ratio appears to be a reliable marker of global anaerobic metabolism and predicts response to DO2 ***,patients likely to benefit from resuscitation can be identified promptly,the P（v-a）CO2/C（a-v）O2 ratio may,therefore,be a useful resuscitation target.

关键词： Cardiac Surgical Procedures Lactic Acid Physiologic Monitoring Resuscitation

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EFFECTS OF INTEGRIN ALPHAⅡb^(R995A) MUTATION ON RECEPTOR AFFINITY AND pp125 (FAK) PHOSPHORYLATION

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Chinese Medical Sciences Journal 2004年第4期19卷 276-281页

作者： Xue-yuanTang Zai-fuJian Guo-pingWang Hong-huiYang weiliu DepartmentofHematology XiangyaHospitalCentralSouthUniversityChangsha410008 DepartmentofHematology theThirdXiangyaHospitalCentralSouthUniversityChangsha410013

Objective To investigate the role of cytoplasmic domain of integrin alphaⅡb in platelet signal transduction. Methods Binding capacity of integrin alphaⅡb R995A to antibody platelet activation complex-1 (PAC-1) and pp125 focal adhesion kinase (FAK) phosphorylation of cells were detected by flow cytometry, immune precipitation, and Western blotting. Results Without activation, wild-type alphaⅡbbeta3 Chinese hamster ovary (CHO) cells failed to bind to PAC-1, but mutant chimera alphaⅡb R995A beta3 CHO cells were able to bind with PAC-1. Furthermore, phosphorylation of pp125 (FAK) in wild-type alphaⅡbbeta3 CHO cells occured only when cells were adhered to fibrinogen, but could not be detected in bovine serum albumin suspension. However in the mutant chimera group, it could be detected in both conditions. Conclusion The mutation in integrin alphaⅡb R995A alters its affinity state as a receptor, thus also mediating cytoplasmic signal transduction leading to the phosphorylation of pp125 (FAK) without ligand binding.

关键词： integrin alphaⅡbbeta3 signal transduction pp125 focal adhesion kinase phosphorylation

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口腔颌面修复重建外科近况

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中国修复重建外科杂志 2006年第4期20卷 339-343页

作者：邱蔚六上海交通大学医学院附属第九人民医院口腔颌面外科上海200011

目的总结口腔颌面修复重建外科的最新进展。方法广泛查阅国内外文献,并进行综合整理分析其主要的创新点。结果从口腔颌面修复重建外科层面,近几年在采用血管化组织移植、人工材料、富血小板血浆、骨牵引成骨、骨折的微创技术治疗、关节... 详细信息

目的总结口腔颌面修复重建外科的最新进展。方法广泛查阅国内外文献,并进行综合整理分析其主要的创新点。结果从口腔颌面修复重建外科层面,近几年在采用血管化组织移植、人工材料、富血小板血浆、骨牵引成骨、骨折的微创技术治疗、关节成形技术、动力性修复以及计算机辅助设计等方面取得显著进展。结论在已取得成绩的基础上,口腔颌面的修复重建外科技术,尤其是个体化治疗,将会有更进一步发展。

关键词：口腔颌面外科临床应用修复重建外科

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乘“双一流”建设东风强一流学科建设——上海交通大学医学院附属第九人民医院口腔颌面外科学建设的思考

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上海交通大学学报（医学版） 2022年第9期42卷 1163-1170页

作者：邱蔚六上海交通大学医学院附属第九人民医院口腔颌面外科上海交通大学口腔医学院国家口腔医学中心国家口腔疾病临床医学研究中心上海市口腔医学重点实验室上海200011

“双一流”建设的中心内容是学科建设。该文通过回顾上海交通大学医学院附属第九人民医院口腔颌面外科的学科发展、取得的成绩,以及学科发展过程中“志存高远”和“改革开放”的2条指导思想,总结出4条学科建设的成功经验:人才培养,梯队... 详细信息

“双一流”建设的中心内容是学科建设。该文通过回顾上海交通大学医学院附属第九人民医院口腔颌面外科的学科发展、取得的成绩,以及学科发展过程中“志存高远”和“改革开放”的2条指导思想,总结出4条学科建设的成功经验:人才培养,梯队建设为先;人才分流,增加溢出效应;倡导学科交叉与交叉学科;推行体制改革与学科创新。此外,该文还对学科建设的未来提出4点建议:宜将“中国式”的口腔颌面外科更名为“中国特色”的口腔颌面外科;以创新为先导,以科研为灵魂;以自然科学与人文科学整合为切入点,以“整合”为指导,培养复合型人才;进一步加强和重视医学中的人文教育。

关键词： “双一流”建设学科建设口腔颌面外科

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