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Acta Pharmacologica Sinica 2005年第12期26卷 1448-1453页

作者： Li-sha XU Li-xia YANG wei-wei HU Xiao yu Li MA Lu-ying LIU Er-qing wei Zhong CHEN~2 Department of Pharmacology School of Medicine,Zhejiang University,Hangzhou 310031,China DepartmentofPharmacology SchoolofMedicineZhejiangUniversityHangzhou310031China

Aim:To investigate the role of histamine in memory deficits induced by MK-801infusion into the ventral hippocampus in ***:An 8-arm radial maze(4arms baited)was used to assess spatial ***:Bilateral ventralintrahippocampal(ih)infusion of MK-801(0.3 μg/site),an N-methyl-D-aspartate(NMDA)antagonist,impaired the retrieval process in both working memory andreference *** injection of histamine(25 or 50 ng/site)orintraperitoneal(ip)injection of histidine(25,50 or 100 mg/kg)markedly amelio-rated the spatial memory deficits induced by *** the histamine H1an-tagonist pyrilamine(0.5 or 1.0 μg/site,ih)and the H2 antagonist cimetidine(2.5 μg/site,ih)abolished the ameliorating effect of histidine( 100 mg/kg,ip)on reference memorydeficits,but not that on working memory deficits induced by ***:The results indicate that histamine in the ventral hippocampus can ameliorateMK-801-induced spatial memory deficits,and that histamine’s effect on referencememory is mediated by postsynaptic histamine H1and H2receptors.

关键词： histamine: maze learning MK-801 working memory reference memory ventral hippocampus histamine H1 receptor

Roles of Salicylic Acid-responsive Cis-acting Elements and W-boxes in Salicylic Acid Induction of VCH3 Promoter in Transgenic Tobaccos

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Acta Biochimica et Biophysica Sinica 2006年第1期38卷 46-52页

作者： Hai-Yan LI~(1,2),wei wei~(2&),and yu LI~(1*) ~1 Institute of Mycology,Jilin Agricultural University,Changchun 130118,China ~2 College of Biotechnology,Jilin Agricultural University,Changchun 130118,China Institute of Mycology Jilin Agricultural University Changchun 130118 China

A salicylic acid (SA)-inducible VCH3 promoter was recently identified from grapevine (Vitisarnurensis) that contains two inverse SA-responsive cis-acting elements and four *** furtherdemonstrate the roles of these elements,four fragments with lengths from-1187,-892,-589,-276 to+7 bp were fused with the β-glucuronidase (GUS) reporter géne and transferred to Nicotiana tobacum,together with another four VCH3 promoter fragments with mutation in the two inverse *** functions,of each promoter fragment were-examined by analysis of GUS activity in thetransgenic tobacco root treated with *** GUS activity was shown in the roots of transgenictobaccos with the VCH3 (-1187)-GUS construct containing two SA-responsive cis-acting elements andfour ***,GUS activity directed by the VCH3 (-892)-GUS construct,containing one SA cis-acting element and four W-boxes,was reduced by up to 35% compared with that in tobaccos transformedwith the VCH3 (-1187)-GUS construct,indicating that the SA cis-acting element plays an important role inSA induction of the VCH3 *** the m2VCH3 (-1187)-GUS nor the mVCH3 (-892)-GUSconstruct,with mutation on the SA-responsive elements,abolished the expression of GUS activity,demon-strating that the W-boxes in the VCH3 promoter are also involved in SA *** arialysis ofGUS activity directed by each of the eight VCH3 promoter fragments showed that GUS was expressedspecifically in vascular *** was concluded that both the SA-responsive cis-acting elements and the W-boxes are important for the SA induction of the VCH3 *** promoter might have a potential usein plant genetic engineering.

关键词： VCH3 promoter salicylic acid(SA)-responsive cis-acting element W-box site-directed mutagenesis SA induction transgenic tobacco

针刺“百会”透“曲鬓”穴拮抗急性脑出血大鼠炎性损伤的机制研究

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针刺研究 2016年第5期41卷 410-416页

作者：陈秋欣邹伟孙晓伟于学平戴晓红牛明明滕伟包宇于薇薇马慧慧黑龙江中医药大学附属第一医院哈尔滨150040 黑龙江中医药大学哈尔滨150040

目的:探讨针刺"百会"透"曲鬓"穴拮抗急性脑出血大鼠炎性损伤的作用机制。方法:54只Wistar大鼠随机分为假手术组、模型组及针刺组,每组按造模后1、3、7d分为3个亚组,每组6只。采用自体血注入法建立脑出血大鼠模型。针刺组取"百会"透患侧... 详细信息

目的:探讨针刺"百会"透"曲鬓"穴拮抗急性脑出血大鼠炎性损伤的作用机制。方法:54只Wistar大鼠随机分为假手术组、模型组及针刺组,每组按造模后1、3、7d分为3个亚组,每组6只。采用自体血注入法建立脑出血大鼠模型。针刺组取"百会"透患侧"曲鬓"进行治疗,24h治疗1次。采用Longa评分法及肢体对称实验评分法对脑出血后大鼠神经功能进行评估,采用免疫组化法检测脑出血组织Toll样受体4(TLR-4)、肿瘤坏死因子(TNF-α)、白介素(IL)-6的阳性表达。结果:模型组大鼠脑出血损伤后出现不同程度的神经功能缺损症状表现,术后3d大鼠神经功能缺损最重;针刺后神经功能缺损明显减轻,针刺组各时间点与模型组比较差异有统计学意义(P<0.01)。在各时间点假手术组大鼠脑组织可见少量TNF-α、TLR-4、IL-6阳性表达;模型组在各时间点TNF-α、TLR-4、IL-6阳性表达均高于假手术组(P<0.01);针刺组在各时间点与模型组比较,TNF-α、TLR-4、IL-6阳性表达明显下调(P<0.01)。TLR-4与IL-6表达呈正相关,TLR-4与TNF-α表达呈正相关。结论:针刺"百会"透"曲鬓"穴可以抑制TLR-4蛋白的表达,降低血肿组织中炎性因子TNF-α、IL-6的含量,减轻脑出血后炎性损伤,改善大鼠神经功能缺损表现。

关键词：针刺(透刺) 脑出血肿瘤坏死因子-α 白介素-6 Toll样受体4

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双酶连续催化生物合成D-丙氨酸

精细化工 2017年第10期34卷 1132-1138,1160页

作者：张卫卫魏宇张宏娟焦庆才刘均忠医药生物技术国家重点实验室南京大学生命科学学院江苏南京210093 南京医科大学药学院江苏南京210029

建立了天冬氨酸消旋酶和D-氨基酸转氨酶双酶连续催化制备D-丙氨酸的方法。利用天冬氨酸消旋酶全细胞催化L-天冬氨酸消旋得到DL-天冬氨酸,离心去除天冬氨酸消旋酶全细胞后升温灭活残留的游离天冬氨酸消旋酶,再加入经镍柱亲和纯化的D-氨... 详细信息

建立了天冬氨酸消旋酶和D-氨基酸转氨酶双酶连续催化制备D-丙氨酸的方法。利用天冬氨酸消旋酶全细胞催化L-天冬氨酸消旋得到DL-天冬氨酸,离心去除天冬氨酸消旋酶全细胞后升温灭活残留的游离天冬氨酸消旋酶,再加入经镍柱亲和纯化的D-氨基酸转氨酶酶液,催化D-天冬氨酸(D-Asp)和丙酮酸(PA)经转氨反应生成D-丙氨酸。经单因素实验得到天冬氨酸消旋酶最佳催化条件为:反应温度40℃,0.2 mol/L磷酸钾缓冲溶液(pH=7.0),底物L-天冬氨酸质量浓度为100 g/L。D-氨基酸转氨酶最佳催化条件为:反应温度42℃,0.2 mol/L磷酸钾缓冲液(pH=7.0),4 mmol/L磷酸吡哆醛,DL-天冬氨酸质量浓度为50 g/L,底物n(PA)∶n(D-Asp)=1∶10。转化产物经等电点结晶和阳离子树脂分离得到D-丙氨酸。在该条件下,D-天冬氨酸转化率达94%,D-丙氨酸收率为84%,对映体过量值(ee值)=98%。

关键词：双酶连续催化天冬氨酸消旋酶 D-氨基酸转氨酶 D-丙氨酸生物工程

父亲参与的现状及其与幼儿社会能力的关系——母亲教养效能的中介作用

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北京师范大学学报（社会科学版） 2017年第5期 49-58页

作者：李晓巍魏晓宇北京师范大学教育学部北京100875

以372名3~6岁幼儿及其父母为被试,采用问卷法考察父亲参与的现状及其与幼儿社会能力的关系,并探究母亲教养效能在父亲参与与幼儿社会能力之间的中介作用。结果表明:(1)父亲参与在生活指导、学习指导两维度得分较高,而在家庭作业指导、... 详细信息

以372名3~6岁幼儿及其父母为被试,采用问卷法考察父亲参与的现状及其与幼儿社会能力的关系,并探究母亲教养效能在父亲参与与幼儿社会能力之间的中介作用。结果表明:(1)父亲参与在生活指导、学习指导两维度得分较高,而在家庭作业指导、家园沟通两维度得分较低,即父亲更多地参与幼儿家庭教育,较少参与学校教育。(2)父亲参与在各个维度上的得分均显著低于母亲参与。(3)建构的结构方程模型发现,除模型M1(父亲生活指导)外,母亲教养效能在父亲参与与幼儿社会能力之间起完全中介作用。

关键词：父亲参与社会能力母亲教养效能幼儿

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“华夷秩序”研究的历史演进及其启示

东北师大学报（哲学社会科学版） 2017年第1期 92-99页

作者：张微微于海洋东北师范大学政法学院吉林长春130117

针对历史上曾长期主导东亚的华夷秩序,中外学者在研究方法和内容上存在着很大区别。从内容上讲,西方国家和日朝越等国学者分别从华夷秩序与西方关系、华夷秩序内部的华夷之辨角度来解释其历史逻辑。中国学者则经历了尊崇、质疑、否定到... 详细信息

针对历史上曾长期主导东亚的华夷秩序,中外学者在研究方法和内容上存在着很大区别。从内容上讲,西方国家和日朝越等国学者分别从华夷秩序与西方关系、华夷秩序内部的华夷之辨角度来解释其历史逻辑。中国学者则经历了尊崇、质疑、否定到重建等不同阶段。从方法上看,历史学研究注重解释"是什么"的问题,而国际关系理论的研究则具有强烈的现实情怀,对史料的选择性使用也招来很多批判。通过梳理华夷秩序研究中的观点和方法,可以看出政治文化上高度的歧视性和统治结构的松散,形成华夷秩序内外部变化的重要线索。中国在参与和塑造未来国际和地区秩序的时候,也要与等级制的华夷观划清界限,坚持古典华夷秩序中宝贵的高度灵活的协商规范。

关键词：华夷秩序宗藩制度朝贡体系协商规范

Precise gradient separation of nanoparticles using reduced graphene oxide-polyoxomolybdate membranes

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Science China Chemistry 2025年第01期 209-216页

作者： Yixin Yang Wanlei Zhao Ruoxuan Zheng Hongqiang Li wei Chen yu-Fei Song State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Quzhou Institute for Innovation in Resource Chemical Engineering

The purification and size sieving of nanoparticles （NPs） represents a significant scientific ***,the r GO-polyoxometalates (POMs=PMo12,Mo132and Mo368) membranes were fabricated successfully via a simple and scalable POMsassisted in-situ photoreduction *** the mass ratio of r GO to PMo12was 1:30,the resultant r GO-PMo12membranes with highly ordered and uniform nanochannels of 1.0 nm can be obtained,which showed an accurate carbon quantum dots（CQDs） separation with a cut-off size approximately 1.0 *** increase of the size of the interlayered POMs from PMo12（1.0 nm）,{Mo132}（2.9 nm） to{Mo368}（3.8 nm）,the resultant r GO-PMo12,r GO-Mo132and r GO-Mo368exhibited precise gradient separation of CQDs and Au nanoparticles by size exclusion *** results pave a promising way to construct new twodimensional （2D） membranes for highly efficient size-selective separation.

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基于遗传算法的网格密码资源调度策略

计算机工程与设计 2016年第11期37卷 3107-3111页

作者：叶伟伟欧庆于海军工程大学信息安全系湖北武汉430033

针对网格资源调度算法中不考虑服务请求实际需要的问题,按照用户对响应时间的实际需要,将服务请求划分成不同等级,以用户满意度为衡量指标,考虑系统的服务能力,兼顾系统负载平衡,利用遗传算法按需调度,更加高效合理地利用系统,使用户能... 详细信息

针对网格资源调度算法中不考虑服务请求实际需要的问题,按照用户对响应时间的实际需要,将服务请求划分成不同等级,以用户满意度为衡量指标,考虑系统的服务能力,兼顾系统负载平衡,利用遗传算法按需调度,更加高效合理地利用系统,使用户能够即时、高效、安全、灵活地获取资源。实验结果表明,该方法能够结合用户请求的实际需求,合理调度,更加充分利用系统资源,在性能上比传统算法有较大提高。

关键词：遗传算法信息网格密码保障资源调度用户满意度系统平衡

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标准模型下基于身份的认证密钥协商协议

计算机工程与设计 2017年第8期38卷 2071-2075页

作者：叶伟伟欧庆于海军工程大学信息安全系湖北武汉430033

目前的大多数认证密钥协商协议安全属性不全面,且多数只能在随机预言模型下证明安全性,为此提出一种基于身份的认证密钥协商协议,具备抗密钥泄露伪装攻击、完全前向安全性、未知密钥共享安全等所有必要的安全属性。在标准模型下将协议... 详细信息

目前的大多数认证密钥协商协议安全属性不全面,且多数只能在随机预言模型下证明安全性,为此提出一种基于身份的认证密钥协商协议,具备抗密钥泄露伪装攻击、完全前向安全性、未知密钥共享安全等所有必要的安全属性。在标准模型下将协议的安全性规约到判定性q-ABDHE数学困难假设,协议对比结果表明,该协议的计算效率有所提高,安全性更强。

关键词：基于身份认证密钥协商强安全标准模型 q-ABDHE数学问题

[Advances in molecular networking technology for discovering emerging contaminants and transformation products]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 33-42页

作者： Xiao-Mei Tan yu-wei Zhang Zhao-yu Jiao Nan-Yang yu Si wei State Key Laboratory of Pollution Control & Resource Reuse School of Environment Nanjing University Nanjing 210046 China.

Emerging contaminants and their transformation products are widely distributed in the environment. These pollutants carry unknown risks owing to their persistence, migration, and toxicity. The wide variety and complex structures of these substances render them difficult to identify using only target analysis. Suspect screening analysis can identify more substances than target analysis in a single run. However, this analysis method is based on limited data and cannot meet the growing demand for compound identification, especially for emerging contaminants and their transformation products with unknown information. The development of high-resolution mass spectrometry technology has promoted the applications of nontarget analysis in the environmental field, especially for identifying unknown transformation products. At present, the challenges of nontarget analysis include the difficulty of finding compounds of interest and their transformation products from complex data. Molecular networking calculates the similarity between mass spectra based on an improved cosine similarity algorithm. This method can cluster molecular families with similar structures, achieve visualization and a collection of massive mass spectral datasets, and promote the annotation of pollutants through networks and communities. Molecular networking can globally organize and systematically interpret complex tandem mass spectral datasets, providing a new direction for nontarget analysis. This technology was first used in proteomics and gradually introduced into metabolomics for the discovery of new natural products. Recently, it has been introduced into the environmental field for the study of various man-made chemicals, particularly for the discovery of emerging contaminants and their transformation products. In this paper, we introduce a molecular networking analysis method based on high-resolution tandem mass spectrometry and describe its applications in the nontargeted screening of emerging cont

关键词： emerging contaminants high-resolution tandem mass spectrometry molecular networking transformation products