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Enriched environment elevates expression of growth associated protein-43 in the substantia nigra of SAMP8 mice

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Neural Regeneration Research 2018年第11期13卷 1988-1994页

作者： Zhen-Yun Yuan Jie Yang Xiao-wei Ma Yan-Yong wang ming-wei wang The First Hospital of Hebei Medical University Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province Shijiazhuang Hebei ProvinceChina

An enriched environment protects dopaminergic neurons from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）-induced neuronal injury, but the underlying mechanism for this is not clear. Growth associated protein-43（GAP-43） is closely associated with neurite outgrowth and axon regeneration during neural development. We speculate that an enriched environment can reduce damage to dopaminergic neurons by affecting the expression of GAP-43. This study is designed to test this hypothesis. Three-month-old female senescence-accelerated mouse prone 8（SAMP8） mice were housed for 3 months in an enriched environment or a standard environment. These mice were then subcutaneously injected in the abdomen with 14 mg/kg MPTP four times at 2-hour intervals. Morris water maze testing demonstrated that learning and memory abilities were better in the enriched environment group than in the standard environment group. Reverse-transcription polymerase chain reaction, immunohistochemistry and western blot assays showed that m RNA and protein levels of GAP-43 in the substantia nigra were higher after MPTP application in the enriched environment group compared with the standard environment group. These findings indicate that an enriched environment can increase GAP-43 expression in SAMP8 mice. The upregulation of GAP-43 may be a mechanism by which an enriched environment protects against MPTP-induced neuronal damage.

关键词： nerve regeneration Parkinson's disease neural plasticity senescence-accelerated mouse prone 8 growth associated protein-43 substantia nigra learning and memory neural regeneration

Development of the fast arcing protection for ECRH system on the HL-2A tokamak

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核聚变与等离子体物理 2007年第1期27卷 44-47页

作者： LI Xiu-juan RAO Jun wang ming-wei wang Xue-yun LI Bo Southwestern Institute of Physics Chengdu 610225

1 Introduction A series of engineering tests and physical experiments,including ECRH[1],was carried out on the HL-2A tokamak in 2005.A fast arcing protection system[2] was developed to ensure operating of ECRH facilit... 详细信息

关键词：弧阈值响应时间托卡马克装置

A high-throughput screening assay for eukaryotic elongation factor 2 kinase inhibitors

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Acta Pharmaceutica Sinica B 2016年第6期6卷 557-563页

作者： Ting Xiao Rui Liu Christopher G.Proud ming-wei wang The National Center for Drug Screening and the CAS Key Laboratory of Receptor Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Department of Pharmacy Medical College of Nanchang University Center for Biological Sciences Life Sciences Building University of Southampton South Australian Health and Medical Research Institute

Eukaryotic elongation factor 2 kinase (eEF2K) inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was obtained from an Escherichia coli expression system and a luminescence-based high-throughput screening (HTS) assay was developed using MH-1 peptide as the substrate. The luminescent readouts correlated with the amount of adenosine triphosphate remaining in the kinase reaction. This method was applied to a large-scale screening campaign against a diverse compound library and subsequent confirmation studies. Nine initial hits showing inhibitory activities on eEF2K were identified from 56,000 synthetic compounds during the HTS campaign, of which, five were chosen to test their effects in cancer cell lines. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND

关键词： eEF2K Inhibito High-throughput screening Luminescenc MH-1 peptide

Serum and glucocorticoid inducible protein kinases(SGKs):a potential target for cancer intervention

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Acta Pharmaceutica Sinica B 2018年第5期8卷 767-771页

作者： Rajesh Basnet Grace Qun Gong Chenyao Li ming-wei wang The National Center for Drug Screening and the CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of Sciences (CAS) University of Chinese Academy of Sciences Department of Molecular Medicine and Pathology The University of Auckland School of Pharmacy Fudan University

The serum and glucocorticoid inducible protein kinase(SGK) family members share similar structure, substrate specificity and function with AKT and signal downstream of the phosphatidylinositol 3-kinase(PI3K) signalling pathway. They regulate a range of fundamental cellular processes such as cell proliferation and survival, thereby playing an important role in cancer development. This perspective intends to give an overview on the involvement of SGKs(particularly SGK3) in cancer progression, and compares the actions of SGK3 and AKT in cell cycle regulation, oncogenic signalling, and the potential as a therapeutic target for cancer.

关键词： SGK AKT PI3K Cancer Signalling Kinase Inhibitor

Scale-aware shape manipulation

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Journal of Zhejiang University-Science C(Computers and Electronics) 2014年第9期15卷 764-775页

作者： Zheng LIU wei-ming wang Xiu-ping LIU Li-gang LIU School of Mathematical Sciences University of Science and Technology of China School of Mathematical Sciences Dalian University of Technology

A novel representation of a triangular mesh surface using a set of scale-invariant measures is *** measures consist of angles of the triangles(triangle angles) and dihedral angles along the edges(edge angles)which are scale and rigidity independent. The vertex coordinates for a mesh give its scale-invariant measures, unique up to scale, rotation, and translation. Based on the representation of mesh using scale-invariant measures, a two-step iterative deformation algorithm is proposed, which can arbitrarily edit the mesh through simple handles *** algorithm can explicitly preserve the local geometric details as much as possible in different scales even under severe editing operations including rotation, scaling, and shearing. The efficiency and robustness of the proposed algorithm are demonstrated by examples.

关键词： Differential coordinates Scale-invariant measures Surface deformation

Noble metal-based high-entropy alloys as advanced electrocatalysts for energy conversion

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Rare Metals 2021年第9期40卷 2354-2368页

作者： Yu-Chen Qin Feng-Qi wang Xin-ming wang ming-wei wang Wen-Long Zhang Wan-Kai An Xiao-Peng wang Yun-Lai Ren Xin Zheng Dong-Can Lv Ayyaz Ahmad College of Sciences Henan Agricultural UniversityZhengzhou 450000China State-Key Laboratory of Chemical Engineering and Shanghai Key Laboratory of Multiphase Materials Chemical EngineeringEast China University of Science and TechnologyShanghai 200237China Department of chemical Engineering MNS University of Engineering and Technology Multan 60000Pakistan

Noble metal-based high-entropy alloy nanoparticles(NM-HEA NPs) have exhibited brilliant catalytic performance toward electrocatalytic energy conversion and attracted increasing attention. The near-equimolar mixed elements of NM-HEA NPs may result in the unique properties including cocktail effect, high entropy effect and lattice distortion effect, which are beneficial for improving the catalytic performance and reducing the amount of noble metal. Herein, several advanced NM-HEA NPs as electrocatalysts for energy conversion are systematically summarized. The preparation methods of NM-HEA NPs are evaluated as well as the catalytic properties and mechanism are discussed classified by electrocatalytic reactions. Finally,the challenges and prospects in this field are carefully discussed. This review provides an overview on recent advances of NM-HEA electrocatalysts for energy conversion and draws more attention in this infant research field.

关键词： Noble metal-based high-entropy alloys Electrocatalysis Energy conversion Synthesis Catalytic performance

Nomograms for Predicting Cancer-Specific Survival of Patients with Gingiva Squamous Cell Carcinoma:A Population-Based Study

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Current Medical Science 2021年第5期41卷 953-960页

作者： Si-si YANG Xiao-huan ZHONG Hui-xin wang An-jie MIN wei-ming wang Department of Oral and Maxillofacial Surgery Center of StomatologyXicmgya HospitalCentral South UniversityChangsha 410008China Department of Orthodontics Center of StomatologyXiangya Hospital of Central South UniversityChangsha 410008China

The use of the traditional American Joint Committee on Cancer(AJCC)staging system alone has limitations in predicting the survival of gingiva squamous cell carcinoma(GSCC)*** aimed to establish a comprehensive prognostic nomogram with a prognostic value similar to the AJCC ***:Patients were identified from SEER *** were selected by a backward stepwise selection method in a Cox regression model.A nomogram was used to predict cancer-specific survival rates for 3,5 and 10 years in patients with *** basic features of model validation were used to evaluate the performance of the survival model:consistency index(C-index),receiver operating characteristic(ROC)curve,calibration chart,net weight classification improvement(NRI),comprehensive discriminant improvement(IDI)and decision curve analysis(DCA).Results:Multivariate analyses revealed that age,race,marital status,insurance,AJCC stage,pathology grade and surgery were risk factors for *** particular,the C-index,the area under the ROC curve(AUC)and the calibration plots showed good performance of the *** to the AJCC system,NRI and IDI showed that the nomogram has improved ***,the nomogram's 3-year and 5-year and 10-year DCA curves yield net benefits higher than traditional AJCC,whether training set or a validation ***:We developed and validated the first GSCC prognosis nomogram,which has a better prognostic value than the separate AJCC staging ***,the nomogram of this study is a valuable tool for clinical practice to consult patients and understand their risk for the next 3,5 and 10 years.

关键词： nomogram SEER gingiva squamous cell carcinoma cancer-specific survival population-based study

High-throughput screening for small molecule inhibitors of the type-I interferon signaling pathway

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Acta Pharmaceutica Sinica B 2018年第6期8卷 889-899页

作者： Elita Yuliantie Xinchuan Dai Dehua Yang Peter J.Crack ming-wei wang The National Center for Drug Screening and the CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of Sciences (CAS) University of Chinese Academy of Sciences Department of Pharmacology and Therapeutics The University of Melbourne School of Pharmacy Fudan University

Interferons(IFNs) are cytokines with fundamental roles in resistance to infections, cancer and other diseases. Type-I IFNs, interferon α(IFN-α) and interferon β(IFN-β), act through a shared receptor complex(IFNAR) comprised of IFNAR1 and IFNAR2 subunits. Binding of type-I IFN to IFNAR1 will robustly activate Janus activated kinase-signal transducer and activator of transcription(JAK-STAT)signaling pathway. Aberrant activation of the type-I IFN response results in a spectrum of disorders called interferonopathies. The purpose of this research is to develop an assay for high-throughput screening(HTS) of small molecule inhibitors of the type-I IFN signaling pathway. Inhibition of type-I IFN signaling can be beneficial in terms of therapeutic use and understanding the underlying mechanism of action. We report here a HTS campaign with the secreted embryonic alkaline phosphatase(SEAP) reporter gene assay against 32,000 compounds which yielded 25 confirmed hits. These compounds were subsequently characterized for their cytotoxicity, effects on STAT phosphorylation and activities in IFN regulatory factor(IRF) transcription.

关键词： High-throughput screening Interferon α receptor Secreted embryonic alkaline phosphatase JAK-STAT IFN regulatory factor Inhibitor

Thinking process rules extraction for manufacturing process design

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Advances in Manufacturing 2017年第4期5卷 321-334页

作者： Jing-Tao Zhou Xiang-Qian Li ming-wei wang Rui Niu Qing Xu Key Laboratory of Contemporary Design and Integrated Manufacturing Technology Ministry of Education of ChinaNorthwestern Polytechnical University Xi'an 710072People's Republic of China Department of Software and Information Service Research Electronic Technology Information Research Institute Ministry of Industry and Information Technology of the People's Republic of China Beijing 100040 People's Republic of China

To realize the reuse of process design knowledge and improve the efficiency and quality of process design, a method for extracting thinking process rules for process design is proposed. An instance representation model of the process planning reflecting the thinking process of techni- cians is established to achieve an effective representation of the process documents. The related process attributes are extracted from the model to form the related events. The manifold learning algorithm and clustering analysis are used to preprocess the process instance data. A rule extraction mechanism of process design is introduced, which is based on the related events after dimension reduction and clustering, and uses the association rule mining algorithm to realize the similar process information extraction in the same cluster. Through the vectorization description of the related events, the final process design rules are formed. Finally, an example is given to evaluate the method of process design rules extraction.

关键词： Process design rules Process design rules extraction Process planning model Related event Manifold learning