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嗜水气单胞菌J-1株丝氨酸蛋白酶基因克隆与序列分析

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水产学报 2004年第1期28卷 84-88页

作者：储卫华陆承平南京农业大学农业部动物疫病诊断与免疫重点实验室南京农业大学农业部动物疫病诊断与免疫重点实验室江苏南京 210095

根据已发表的气单胞菌胞外蛋白酶基因核苷酸序列,设计和合成了一对引物,以嗜水气单胞菌AhJ-1的基因组DNA为模板,通过PCR技术,扩增到约900bp的丝氨酸蛋白酶基因片段,并克隆到质粒载体pGEM-T中进行测序和分析,结果表明扩增的丝氨酸蛋白酶... 详细信息

根据已发表的气单胞菌胞外蛋白酶基因核苷酸序列,设计和合成了一对引物,以嗜水气单胞菌AhJ-1的基因组DNA为模板,通过PCR技术,扩增到约900bp的丝氨酸蛋白酶基因片段,并克隆到质粒载体pGEM-T中进行测序和分析,结果表明扩增的丝氨酸蛋白酶基因片段与已发表的嗜水气单胞菌丝氨酸蛋白酶Ahe2的同源性有87％,扩增片段编码343个氮基酸,推测的分子量为35700,计算机软件分析表明编码的氨基酸有较高的抗原性,可作为核酸疫苗的侯选基因片段。

关键词：嗜水气单胞菌丝氨酸蛋白酶基因克隆

学科类培训机构缩减对学生在校时长的影响:基于“双减”政策的评估研究

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华东师范大学学报（教育科学版） 2025年第1期43卷 37-48页

作者：陆伟魏易杭州师范大学经亨颐教育学院杭州311121 北京大学中国教育财政科学研究所北京100871

对校外培训机构的治理整顿是“双减”改革的重要内容之一。本文使用大众点评网学科类培训机构的商铺信息,结合高德地图地理编码和搜索POI应用接口,以及全国中小学教育信息化使用情况调查等数据,实证检验了学科类校外培训机构缩减对学生... 详细信息

对校外培训机构的治理整顿是“双减”改革的重要内容之一。本文使用大众点评网学科类培训机构的商铺信息,结合高德地图地理编码和搜索POI应用接口,以及全国中小学教育信息化使用情况调查等数据,实证检验了学科类校外培训机构缩减对学生在校时长的影响。研究发现:(1)“双减”期间,全国层面学科类培训机构的缩减比例约为88%。(2)学校周边学科类培训机构的缩减程度越高,学生每天待在学校内的时间也越多,也即校内教育与校外教育出现一定程度的替代,且这种替代效应在弱势阶层群体中更为明显;在北上广深四个一线城市不明显,但在其他众多二三线城市可以观察到。(3)学校周边学科类培训机构的缩减程度越高,学生在学校内完成课后作业的概率也越高,而在校外辅导班完成课后作业的概率则越低;但培训机构缩减对学生完成课后作业的总时长没有影响。

关键词： “双减” 学科类培训机构校外培训在校时长

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超便携模糊控制的人工鼻设计研究

生物医学工程与临床 2017年第3期21卷 236-241页

作者：冯奇毛伟朱华麟吕维敏浙江医药高等专科学校医疗器械学院浙江宁波315100 宁波市第一医院浙江宁波315010 浙江省医疗器械研究所浙江杭州310009

目的为避免传统人工鼻不能实现理想的干冷空气湿化及温化功能,研制便携式模糊控制人工鼻。方法利用高能量密度锂电池、薄膜加热器件、吸湿纤维对吸入空气进行加湿、加温,用高精数字温湿度传感器对气路出口处的空气温湿度进行测量,并用... 详细信息

目的为避免传统人工鼻不能实现理想的干冷空气湿化及温化功能,研制便携式模糊控制人工鼻。方法利用高能量密度锂电池、薄膜加热器件、吸湿纤维对吸入空气进行加湿、加温,用高精数字温湿度传感器对气路出口处的空气温湿度进行测量,并用单片机对加热、加湿装置进行模糊控制。结果使得吸入到呼吸道的空气在6~8 min内达到34℃、95%相对湿度的理想状态,并保持基本稳定。结论仪器(及里面的湿化液)无论处于何种状态均能正常工作,具有极强的便携性,良好的气道湿化、温化效果。

关键词：模糊控制人工鼻温度湿度

Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan huang Yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li Hai-wei Pu Xin-Yian Guo Jian-Gang Shen Su-hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

Recyclable polythioesters and polydisulfides with near-equilibrium thermodynamics and dynamic covalent bonds

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Science China Chemistry 2023年第3期66卷 725-738页

作者： wei Xiong hua lu Beijing National Laboratory for Molecular Sciences Center for Soft Matter Science and EngineeringKey Laboratory of Polymer Chemistry and Physics of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

The accumulation of discarded petroleum-based plastics causes serious environmental ***,recyclable polymers with neutrality in thermodynamics,such as polyesters,polycarbonates,and polyolefins,have been developed as promising alternatives to traditional petroleum-based ***,the chemical recycle of these polymers usually requires high energy input and expensive *** covalent bonds,such as thioester and disulfide bonds,have emerged as building blocks for constructing recyclable polymers that can be rapidly degraded/recycled under mild *** this review,we introduce representative studies on recyclable polythioesters and polydisulfides with respect to their synthetic strategies,thermodynamic manipulation,physicochemical properties,and preliminary *** also highlight the important role of kinetic factors played in the design of recyclable ***,major challenges,perspectives,and future opportunities in the synthesis and applications of polythioesters/polydisulfides are discussed.

关键词： polythioesters polydisulfides recyclable near-equilibrium thermodynamics dynamic covalent bond

Facile synthesis of the dodecahydridododecaborate (B12H122-) from borane Lewis base adducts

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Science China Chemistry 2025年

作者： Yi Jing Xin-Ran Liu Xing-hua Wang Hui Han Sha-Sha Liang Xi-Meng Chen Dong-Hui wei Lai-Sheng Wang Xue-Nian Chen College of Chemistry Zhengzhou University School of Chemistry and Chemical Engineering Henan Key Laboratory of Boron Chemistry and Advanced MaterialsHenan Normal University Department of Chemistry Brown University

Polyhedral boranes are a class of well-known boron molecular clusters widely used in energy,chemistry,medicine,and materials science because of their unique physical and chemical *** efforts have been made in the past decades to find more effective synthetic methods for this important class of boron ***,existing synthetic methods suffer from low efficiency and low ***,we report a facile one-pot synthesis of[（CH3）3S]2B12H12with moderate yields at mild *** mechanisms for the multi-step chemoselective synthesis of B12H122-without other by-products are elucidated based on theoretical results and our previous *** Lewis base used in B–H bond condensation reaction,which acts as a hydrogen or to balance the newly generated polyhedral borane charges,is proposed and studied in *** current study has led to a more effective and selective synthetic method for B12H122-and has also implicated the syntheses of other new polyhedral boranes.

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考虑ESG行为类别的碳减排与经济增长均衡机制研究

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经济问题 2025年第1期 15-26页

作者：曲卫华孙娜曲国华山西大学经济与管理学院山西太原030006 山西大学管理与决策研究所山西太原030006 山西财经大学管理科学与工程学院山西太原030006

“双碳”目标视域下,探究企业ESG(Environmental、Social and Governance)行为对碳排放的作用机理具有重要意义。考虑ESG行为存在表面上顺应迎合而实质上“言行不一”,构建了划分ESG行为类别的动态随机一般均衡(DSGE)模型,研究实质性ES... 详细信息

“双碳”目标视域下,探究企业ESG(Environmental、Social and Governance)行为对碳排放的作用机理具有重要意义。考虑ESG行为存在表面上顺应迎合而实质上“言行不一”,构建了划分ESG行为类别的动态随机一般均衡(DSGE)模型,研究实质性ESG行为与策略性ESG行为对碳排放等宏观经济因素的动态传导机制。研究发现:相比难以实现碳减排的策略性ESG行为,企业采取实质性ESG行为能够显著降低碳排放量且对宏观经济有正向影响,推动经济向低碳绿色转型,实现减排与经济发展的“共赢”。企业实质性ESG行为对碳减排与经济增长的影响机制为:(1)内部资本投入机制:企业采取实质性ESG行为对碳减排技术研发投入形成溢出效应,且实质性ESG行为激励企业将生产要素投入由高碳产品转移到低碳产品,以实现“增低碳降高碳”,达到碳减排与经济增长的协调统一;(2)外部政策激励机制:企业采取实质性ESG行为可获得更多的政府碳减排补贴,促使企业增加低碳产出投入,实现外部政策激励与内部低碳转型的协同效应;(3)金融机构信贷优惠机制:企业采取实质性ESG行为能够激发金融机构对其实行差异化信贷优惠利率,增加企业碳减排技术的研发投入,推动绿色金融与绿色技术的协同发展。研究为辨识ESG行为类别推动企业低碳转型发展提供理论依据与政策启示。

关键词：实质性ESG行为策略性ESG行为绿色金融碳排放动态随机一般均衡模型

Homologous CoS2 interface with directed electron transfer and strong chloridion repulsion for efficient seawater oxidation

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Science China Chemistry 2025年

作者： Yuan Tian Jiang-Bo Chen Jie Ying Ge Tian Yu-Xuan Xiao Yi lu Si-Ming Wu wei Geng Ling Shen Xiao-Yu Yang State Key Laboratory of Advanced Technology for Materials Synthesis and Processing and School of Materials Science and Engineering and Shenzhen Research Institute & Laoshan Laboratory Wuhan University of Technology School of Chemical Engineering and Technology Sun Yat-Sen University National Energy Key Laboratory for New Hydrogen-Ammonia Energy Technologies Foshan Xianhu Laboratory

Seawater electrolysis is a highly promising method to produce high energy density hydrogen,especially in dry areas that have scarce freshwater ***,application of seawater electrolysis is confronted by two major problems associated with catalysts employed for the anodic oxygen evolution reaction （OER） including the competitive hypochlorite evolution reaction（HCER） and strong chloridion （Cl–） triggered *** studies aimed at overcoming these problems,a facile method has been developed to synthesize a homologous CoS2heterojunction as an OER *** to a difference in work functions at the interface and increased electron density,this material has directed electron transfer and strong chloridion repulsion properties,*** have shown that CoS2achieves low overpotentials of 289 and 322 mV at 10 mA cm-2current density in respective alkaline （1 M KOH） and alkaline simulated seawater ***,the outstanding stability of CoS2is reflected in a negligible decline in activity during promotion of the OER for 100 *** of density functional theory calculations reveal that the homologous CoS2heterojunction promotes directed electron transport for optimizing adsorption/desorption of reactive species and heightens the Cl-adsorption energy for strongly repulsing Cl-,phenomena that enhance OER performance in alkaline simulated seawater.

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Lipomatous ependymoma with ZFTA:RELA fusion-positive:A case report

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World Journal of Clinical Cases 2025年第1期13卷 48-55页

作者： Xiao-Yu Zhao Juan-Han Yu Yi-hua Wang Yi-Xin Liu lu Xu Lin Fu Ning Yi China Medical University The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Department of Pathology The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Anshan Cancer Hospital Anshan 114000Liaoning ProvinceChina

BACKGROUND Ependymoma with lipomatous differentiation is a rare type of *** ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of central nervous system *** fusion-positive lipomatous ependymoma has not been reported to *** SUMMARY We reported a case of a 15-year-old Chinese male who had a sudden convulsion lasting approximately six *** resonance imaging showed a round cystic shadow of approximately 1.9 cm×1.5 cm×1.9 cm under the right parieto-occipital *** examination showed characteristic perivascular pseudorosettes and adipose differentiation in the ***-chemical staining showed that the tumor cells were negative for cytokeratin,NeuN,Syn and p53,but positive for GFAP,vimentin and S-100 ***-ficant punctate intracytoplasmic EMA immunoreactivity was *** level of Ki-67 was about 5%.Genetic analysis revealed ZFTA:RELA fusion.A cranio-tomy with total excision of the tumor was *** follow-up time was 36 months,no evidence of disease recurrence was found in magnetic resonance *** Based on these findings,the patient was diagnosed as a ependymoma with ZFTA fusion and lipomatous *** case report provides information on the microscopic morphological features of ependymoma with ZFTA fusion and lipomatous differentiation,which can help pathologists to make a definitive diagnosis of this tumor.

关键词： Ependymoma Lipomatous Molecular classification Supratentorial ZFTA:RELA fusion Case report