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A Large Gap of Atmospheric Pressure RF-DBD Glow Discharges in Ar and Mixed Gases

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Chinese Physics Letters 2011年第1期28卷 142-144页

作者： LI Bin CHEN Qiang LIU zhong-wei wang Zheng-Duo Laboratory of Plasma Physics and Alaterials Beijing Institute of Graphic Communication Beijing 102600

A large gap （up to 5.5 mm） between electrodes is acquired at Ar atmospheric pressure glow discharge （APGD） in radio frequency dielectric barrier discharge （RF-DBD）. The characteristics of I - V in single barrier... 详细信息

关键词： Plasma physics

Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats

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Neuroscience Bulletin 2019年第1期35卷 67-78页

作者： Yu Deng Xing Tan Miao-Ling Li wei-zhong wang Yang-Kai wang Department of Physiology Second Military Medical UniversityShanghai 200433China Institution of Polar Medicine Research Center Second Military Medical UniversityShanghai 200433China Department of Anesthesiology Changhai HospitalShanghai 200433China Institute of Cardiovascular Medical Research Southwest Medical UniversityLuzhou 646000China

The rostral ventrolateral medulla(RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensinconverting enzyme 2(ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats(SHRs).Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs.

关键词： Hypertension Renin-angiotensin system Gene transfer Acetylcholine Sympathetic nerve activity

Effect of p27^(KIP1) on cell cycle and apoptosis in gastric cancer cells

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World Journal of Gastroenterology 2005年第45期11卷 7072-7077页

作者： Jian-Yong Zheng wei-zhong wang Kai-Zong Li Wen-Xian Guan wei Yan Department of Gastrointestinal Surgery Xijing Hospital FourthMilitary Medical University Xi'an 710032 Shaanxi ProvinceChina Department of Hepatobiliary Surgery XijingHospital Fourth Military Medical University Xi'an 710032Shaanxi Province China Department of Pathology Fourth Military MedicalUniversity Xi'an 710032 Shaanxi Province China

AIM： To elucidate the effect of p27^KIP1 on cell cycle and apoptosis regulation in gastric carcinoma cells. METHODS： The whole length of p27^KIP1 cDNA was transfected into human gastric cancer cell line SCG7901 by lipofectamine. Expression of p27^KIP1 protein or mRNA was analyzed by Western blot and RNA dot blotting, respectively. Effect of p27^KIP1 on cell growth was observed by MTT assay and anchorage-independent growth in soft agar. Tumorigenicity in nude mice was used to assess the in vivo biological effect of p27^KIP1. Flow cytometry, TUNEL, and electron microscopy were used to assess the effect of p27^KIP1 on cell cycle and apoptosis. RESULTS： Expression of p27^KIP1 protein or mRNA increased evidently in SCG7901 cells transfected with p27^KIP1. The cell growth was reduced by 31% at 48 h after induction with zinc determined by cell viability assay. The alteration of cell malignant phenotype was evidently indicated by the loss of anchorage-independent growth ability in soft agar. The tumorigenicity in nude mice was reduced evidently （0.55±0.14 cm vs 1.36±0.13cm, P〈0.01）. p27^KIP1 overexpression caused cell arrest with 36% increase （from 33.7% to 69.3%, P〈0.01） in G1 population. Prolonged p27^KIP1 expression induced apoptotic cell death reflected by pre-G1 peak in the histogram of FACS, which was also confirmed by TUNEL assay and electron microscopy. CONCLUSION： p27^KIP1 can prolong cell cycle in G1 phase and lead to apoptosis, p27^KIP1 may be a good candidate for cancer gene therapy.

关键词： Cell cycle Apoptosis Gastric neoplasm p27^KIP1

Local Uniqueness of Weak Solutions for a Class of Quasilinear Subelliptic Equations

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Journal of Mathematical Research and Exposition 2011年第2期31卷 295-302页

作者： Xue wei CUI Yong zhong wang Department of Applied Mathematics Northwestern Polytechnical University Shaanxi 710072 P. R. China

In this note,we obtain some a-priori estimates for gradient of weak solutions to a class of subelliptic quasilinear equations constructed by Ho¨rmander’s vector fields,and then prove local uniqueness of weak solutio... 详细信息

关键词： Hrmander’s vector fields subelliptic weak solution uniqueness

Overexpression of Bax induces apoptosis and enhances drug sensitivity of hepatocellular cancer-9204 cells

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World Journal of Gastroenterology 2005年第23期11卷 3498-3503页

作者： Jian-Yong Zheng Guang-Shun Yang wei-zhong wang Wen-Xian Guan Jiang Li Wen-Liang wang Kai-Zong Li Department of Laparoscope Eastern HepatobiliarySurgery HospitalSecond Military Medical UniversityShanghai 200438China Department of Gastrointestinal Surgery Xijing HospitalFourth Military Medical UniversityXi'an 710032Shaanxi ProvinceChina Department of Pathology Fourth Military Medical UniversityXi'an 710032Shaanxi ProvinceChina Department of Hepatobiliary Surgery Xijing HospitalFourth Military Medical UniversityXi'an 710032Shaanxi ProvinceChina

AIM: To investigate the role of overexpression of Bax in apoptotic pathways and the response of human hepatocellular cancer (HCC)-9204 cells to cell death induced by ***: The whole length of Bax cDNA was transfectrd into human HCC-9204 cells by the method of lipofectamine transfection. An inducible MT-Ⅱ regulatory system was constructed, which allowed controlled expression of protein upon addition of ZnSO4 (100 μmol/L) as an external inducer. Stable transfecting inducible expression vector containing Bax gene was performed. Expression of Bax in protein was analyzed by immunohistochemistry and Western blotting. TUNEL and flow cytometry were used to assess the effect of Bax on apoptosis. Colony assay and tetrazolium blue (MTT) assay were used to evaluate the difference in drug sensitivity of HCC-9204 cells after ***: Immunohistochemistry and Western blotting demonstrated that the expression of Bax protein markedly increased in Bax-transfected cells 4 h after the addition cytoplasm and perinuclear region of HCC-9404 cells, and there was ectopic expression in cells with marked condensation of chromatin and cytoplasm (apoptotic cells). Apoptotic index significantly increased in Bax-transfected HCC-9204/Bax cells (3.6 vs 27.2, 4.2 vs 32.3, P＜0.05).Flow cytometry analysis showed a significant sub-G1 peak and apoptosis in 15.4% HCC-9204/Bax cells 24 h after treatment. Furthermore, colony survival rate decreased from 66% (HCC-9204/pMD) to 45% (HCC-9204/Bax) 2 dafter ADR withdrawal. MTT assay result showed that the effects of Bax on cell viability following ADR exposure were significant as compared to the vehicle-transfected HCC-9204/pMD cells (21% vs 44%, P＜0.01).CONCLUSION: Overexpression of Bax not only induces apoptosis, but also sensitizes HCC-9204 cells to cell death induced by adriamycin.

关键词： Bax Apoptosis Hepatocellular carcinoma

Diagnosis and treatment of acute rejection in the first case of human living-related small bowel transplantation with a long-term survival in China

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World Journal of Gastroenterology 2005年第34期11卷 5332-5335页

作者： wei-Liang Song wei-zhong wang Guo-Sheng Wu Meng-Bin Li Ji-Peng Li Gang Ji Guang-Long Don Hong-wei Zhang Department of Gastrointestinal Surgery Xijing HospitalFourth Military Medical University Xi'an 710032 Shaanxi Province China

AIM： To report the comprehensive diagnosis and treatment of acute rejection in the first case of living-related small bowel transplantation with a long-term survival in China. METHODS： A 18-year-old boy with short gut syndrome underwent living-related small bowel transplantation, with the graft taken from his father （44-year old）. A segment of 150-cm distal small bowel was resected from the donor. The ileo-colic artery and vein from the donor were anastomosed to the infrarenal aorta and vena cava of the recipient respectively. The intestinal continuity was restored with an end-to-end anastomosis between the recipient jejunum and donor ileum, and the distal end was fistulized. FK506, MMF and prednisone were initially used for post-transplant immunosuppression. Endoscopic observation and mucosal biopsies of the graft were carried out through the terminal ileum enterostomy; serum was collected to detect the levels of IL-2R, IL-4, IL-6 and IL-8. The change of the graft secretion and absorption was observed. RESULTS： Acute rejection was diagnosed promptly and cured. The patient was in good health, 5 years after living- related small bowel transplantation. CONCLUSION： The correct diagnosis and treatment of acute rejection are the key to the long-term survival after living-related small bowel transplantation.

关键词： Rejection Comprehensive diagnosis Small bowel transplantation

Regain of sex determination system and sexual reproduction ability in a synthetic octoploid male fish

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Science China(Life Sciences) 2021年第1期64卷 77-87页

作者： Meng Lu Xi-Yin Li Zhi Li Wen-Xuan Du Li Zhou Yang wang Xiao-Juan Zhang zhong-wei wang Jian-Fang Gui State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesWuhan430072China University of Chinese Academy of Sciences Beijing100049China

Polyploids in vertebrates are generally associated with unisexual reproduction,but the direct consequences of polyploidy on sex determination system and reproduction mode remain ***,we synthesized a group of artificial octoploids between unisexual gynogenetic hexaploid Carassius gibelio and sexual tetraploid Carassius *** synthetic octoploids were revealed to have more than 200 chromosomes,in which 50 chromosomes including the X/Y sex determination system were identified to transfer from sexual tetraploid *** into the unisexual gynogenetic hexaploid ***,a few synthetic octoploid males were found to be fertile,and one octoploid male was confirmed to regain sexual reproduction ability,which exhibits characteristics that are the same to sexual reproduction tetraploid males,such as 1:1 sex ratio occurrence,meiosis completion and euploid sperm formation in spermatogenesis,as well as normal embryo development and gene expression pattern during ***,the current finding provides a unique case to explore the effect of sex determination system incorporation on reproduction mode transition from unisexual gynogenesis to sexual reproduction along with genome synthesis of recurrent polyploidy in vertebrates.

关键词： polyploidy synthetic polyploid unisexual reproduction sexual reproduction sex determination system sex chromosome

Design and fabrication of an surface acoustic wave resonator based on AlN/4H-SiC material for harsh environments

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2017年第1期18卷 67-74页

作者： wei-zhong wang Ji LIANG Yong RUAN wei PANG Zheng YOU Collaborative Innovation Center for Micro/Nano Fabrication Device and System State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision Instrument Tsinghua University Beijing 100084 China State Key Laboratory of Precision Measurement Technology and lnstruments Tianjin University Tianjin 300072 China

Surface acoustic wave （SAW） sensors and micro-electromechanical system （MEMS） technology provide a promising solution for measurement in harsh environments such as gas turbines. In this paper, a SAW resonator （size： 1107μm× 721 μm） based on the AlN/4H-SiC multilayer structure is designed and simulated. A MEMS-compatible fabrication process is employed to fabricate the resonator. The results show that highly c-axis-oriented AlN thin films deposited on the 4H-SiC substrate are obtained, with that the diffraction peak of AlN is 36.10° and the lowest full width at half maximum （FWHM） value is only 1.19°. The test results of the network analyzer are consistent with the simulation curve, which is very encouraging and indicates that our work is a significant attempt to solve the measurement problems mainly including high temperature stability of sensitive structures and the heat transmission of leads in harsh environments. It is essential to get the best performance of SAW resonator, optimize and characterize the behaviors in high temperatures in future research.

关键词： Surface acoustic wave (SAW) resonator AlN/4H-SiC Harsh environment Micro-electromechanical system(MEMS) technology Gas turbine

Design and Synthesis of a Novel Peptidomimetic Inhibitor of Caspase-3

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Chemical Research in Chinese Universities 2006年第2期22卷 225-228页

作者： ZHANG Jin-liang LIU Lin-hua GUO Yang-hong YANG Jin-gang LI wei zhong Da-fang wang Ji-dong College of Life Science Jilin University Changchun 130021 P. R. China

Caspases, a family of cysteine proteases, comprise of highly homologous enzymes that play an important role in apoptotic cell death. Caspase-3 shows key functions in apoptosis, mediating apoptotic cascade from the intrinsic and extrinsic activation pathways. Therefore, caspase-3 is an attractive target for therapeutic intervention. For instance, inhibitors of caspase-3 have been described as promising cardioprotectants, neuroprotectants and antiarthritic agents. A novel peptidomimetic inhibitor of caspase-3, has been designed, which still has the properties of a reversible inhibitor, while the P1 site at the C-terminal remains, and only L-amino acid has been replaced by D-amino acid. Also presented here is the synthesis of the inhibitor and its inhibitory activity against caspase-3, which was tested by the fluorescent activity assay.

关键词： Caspase-3 Inhibitor Retro-inverso Peptidomimetie Synthesis