检索结果-南通市图书馆

Journal of Geriatric Cardiology 2024年第2期21卷 242-245页

作者： wei CHEN Kun HUANG wei-wei guo Fan ZHOU De-Ning LIAO Department of Cardiology Shanghai Jingan District Zhabei Central HospitalShanghaiChina Department of Cardiology Shanghai Changzheng HospitalShanghaiChina Department of Hematology Shanghai Jingan District Zhabei Central HospitalShanghaiChina

Cardiac tumors are rare.However,cardiac metastases can occur in up to 10%of patients with cancer.Among cardiac neoplasms,metastases are much more common than primary cardiac tumors.[1]Metastatic cardiac neoplasms most... 详细信息

关键词： neoplasms sustained cardiac

Genetically modified pigs:Emerging animal models for hereditary hearing loss

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2024年第2期45卷 284-291页

作者： Xiao Wang Tian-Xia Liu Ying Zhang Liang-wei Xu Shuo-Long Yuan A-Long Cui wei-wei guo Yan-Fang Wang Shi-Ming Yang Jian-guo Zhao State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Savaid Medical School University of Chinese Academy of SciencesBeijing 100049China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China Department of Otolaryngology-Head and Neck Surgery Institute of OtolaryngologyChinese PLA General HospitalBeijing 100853China Division of Life Sciences and Medicine University of Science and Technology of ChinaHefeiAnhui 230022China State Key Laboratory of Animal Biotech Breeding Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China

Hereditary hearing loss(HHL),a genetic disorder that impairs auditory function,significantly affects quality of life and incurs substantial economic losses for society.To investigate the underlying causes of HHL and evaluate therapeutic outcomes,appropriate animal models are necessary.Pigs have been extensively used as valuable large animal models in biomedical research.In this review,we highlight the advantages of pig models in terms of ear anatomy,inner ear morphology,and electrophysiological characteristics,as well as recent advancements in the development of distinct genetically modified porcine models of hearing loss.Additionally,we discuss the prospects,challenges,and recommendations regarding the use pig models in HHL research.Overall,this review provides insights and perspectives for future studies on HHL using porcine models.

关键词： Pigs Animal models Hereditary hearing loss Genetic modification Inner ear

Strengthening pharmacotherapy research for COVID-19-induced pulmonary fibrosis

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第5期12卷 875-879页

作者： Yan-Miao Liu Jing Zhang Jing-Jing Wu wei-wei guo Fu-Shan Tang The First Clinical Institute Zunyi Medical UniversityZunyi 563006Guizhou ProvinceChina Key Laboratory of Clinical Pharmacy in Zunyi City Zunyi Medical UniversityZunyi 563006Guizhou ProvinceChina Department of Respiratory Medicine Central Hospital in Jinchang CityJinchang 737102Gansu ProvinceChina Department of Clinical Pharmacy Key Laboratory of Basic Pharmacology of Guizhou Province and School of PharmacyZunyi Medical UniversityZunyi 563006Guizhou ProvinceChina Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical UniversityZunyi 563006Guizhou ProvinceChina

The global spread of severe acute respiratory syndrome coronavirus 2 has resulted in a significant number of individuals developing pulmonary fibrosis(PF),an irreversible lung injury.This condition can manifest within a short inter-val following the onset of pneumonia symptoms,sometimes even within a few days.While lung transplantation is a potentially lifesaving procedure,its limited availability,high costs,intricate surgeries,and risk of immunological rejection present significant drawbacks.The optimal timing of medication administration for coronavirus disease 2019(COVID-19)-induced PF remains controversial.Despite this,it is crucial to explore pharmacotherapy interventions,involving early and preventative treatment as well as pharmacotherapy options for advanced-stage PF.Additionally,studies have demonstrated disparities in anti-fibrotic treatment based on race and gender factors.Genetic mutations may also impact therapeutic efficacy.Enhancing research efforts on pharmacotherapy interventions,while considering relevant pharmacological factors and optimizing the timing and dosage of medication administration,will lead to enhanced,personalized,and fair treatment for individuals impacted by COVID-19-related PF.These measures are crucial in lessening the burden of the disease on healthcare systems and improving patients'quality of life.

关键词： COVID-19 Pulmonary fibrosis Pharmacotherapy intervention Medication administration Timing Dosage

A preliminary study of the mechanism of compound hearing loss caused by ototoxic drugs combined with impulse noise

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

iLABMED 2024年第1期2卷 38-52页

作者： Xin Qiu Qing‐qing Jiang wei‐wei guo Ning Yu Shi‐ming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Chinese PLA Medical School BeijingChina

Background:Hearing loss(HL)is becoming increasingly common and is more commonly caused by noise,ototoxic substances,or a combination of ototoxic factors.However,so far,few studies have examined the mechanism by which compound factors cause HL.The only relevant study is about occupational ototoxic substances combined with environmental noise at 85-110 dB SPL.In this study,to address the shortcomings of existing research,we innovatively focused on HL induced by loud noise(impulse noise,>160 dB SPL)combined with common ototoxic drugs.The aim of this study was to establish and validate a mature animal model,and then to compare the characteristics of audiology,pathomorphology and molecular features,and to preliminarily predict pathogenesis in compound HL.Materials and Methods:We selected guinea pigs to construct in vivo HL model groups for different extents of exposure,including a blank control group,a single-drug group,a single-impulse noise group,and a compound group.The animal model of the mature compound HL group was established using gentamicin combined with impulse noise.We then performed audio-logical and pathological verification.We analyzed the auditory brainstem response(ABR),pathological morphology of the cochlea,and molecules(including important self-radicals,cytokines,and apoptosis signal trans-duction pathway proteins in the pathogenesis of drug-and noise-induced HL),compared the effect of different extents of exposure on HL,and preliminarily predict the pathogenic mechanism of compound HL.Results:Four groups of animal models were established successfully and verified by audiology and pathology.Regarding audiology,there were no sig-nificant differences in the ABR thresholds before exposure(p>0.05),but differences emerged among the groups after exposure.Notably,after 3,7,and 14 days of exposure,there were significant differences in the ABR thresholds between the compound group and both the drug and noise groups(p<0.01),and after 14 days,the HL of the compound group was much more severe(greater than the li

关键词： auditory brainstem response gentamicin hearing loss impulse noise molecular mechanisms pathomorphology

Involvement of NLRP3-inflammasome pathway in noise-induced hearing loss

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2022年第12期17卷 2750-2754页

作者： Na Sai Yuan-Yuan Yang Long Ma Da Liu Qing-Qing Jiang wei-wei guo wei-Ju Han College of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalBeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment for Prevention and Treatment BeijingChina Department of Otolaryngology Head and Neck Surgery Peking University Shenzhen HospitalShenzhenGuangdong ProvinceChina PLA Rocket Force Characteristic Medical Center Department of Stomatology China

The inflammasome is a multiprotein oligomer in the cell cytoplasm and is part of the innate immune system.It plays a crucial role in the pathological process of noise-induced hearing loss(NIHL).However,the mechanisms of NLR family pyrin domain containing 3(NLRP3)inflammasome activation in NIHL have not been clearly demonstrated.In this study,miniature pigs were exposed to white noise at 120 dB(A)and auditory brainstem response measurements were used to measure their hearing function.Immunofluorescence staining,confocal laser scanning microscopy,western blot assay,and quantitative reverse transcription-polymerase chain reaction were used to analyze inflammasome-related protein distribution and expression.NLRP3,interleukin-1β,interleukin-18,and cleaved-caspase-1 were highly expressed in the cochlea after 120 dB(A)white noise exposure.Our findings suggest that NLRP3-inflammasomes in the cochlea may be activated after acoustic trauma,which may be an important mechanism of noise-induced hearing loss.

关键词： acoustic injury inflammasome interleukin-1β miniature pig NLR family pyrin domain containing 3(NLRP3) noise-induced hearing loss sensorineural hearing loss

Molecular Epidemiology and Vaccine Compatibility Analysis of Seasonal Influenza Viruses in Wuhan, 2016–2019

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2020年第5期35卷 556-565页

作者： Liang-Jun Chen Jing-Jing guo wei-wei guo E-Xiang Shen Xin Wang Kai-Ji Li Jie Yan Mang Shi Yi-Rong Li wei Hou Department of Laboratory Medicine Zhongnan HospitalState Key Laboratory of VirologyInstitute of Medical VirologySchool of Basic Medical SciencesWuhan UniversityWuhan 430071China School of Medicine Sun Yat-sen UniversityGuangzhou 510080China Marie Bashir Institute for Infectious Diseases and Biosecurity Charles Perkins CentreSchool of Biological Sciences and Sydney Medical SchoolThe University of SydneySydneyNSW 2006Australia

Influenza viruses(FLUV)cause high morbidity and mortality annually in the world and pose a serious threat to the public health.Wuhan,as an important transportation hub in China,has a dense population and suitable climate,which also lays a major hidden danger for the outbreak of influenza.To survey and characterize the seasonal FLUV in Wuhan during 2016–2019,we collected 44,738 throat swabs,among which 15.5%were influenza A(FLUAV)positive,6.1%influenza B(FLUBV)and 0.3%co-infection.By monitoring FLUV in each month from June 2016 to May 2019,different with the previously seasonality pattern,only a single influenza peak was appeared in winter of 2017–2018 and 2018–2019,respectively.These data indicated that the complex circulation pattern of seasonal influenza in Wuhan.In addition,we found the age group was skewed towards 5–14 years group whose activity were mostly school based,which suggested school may be an important place for influenza outbreaks.Meanwhile,phylogenic analysis revealed that two subtypes(subclade 3C.2 a2 and 3C.2 a1b)of A(H3N2)were circulating in Wuhan and there was an obvious transition in 2018 because the two subclades were detected simultaneously.Furthermore,by estimating the vaccine effectiveness,we found that the vaccine strain of FLUAV didn’t seem to match very well the current epidemic strain,especially A(H3N2).Hence,more accurate prediction of seasonal outbreak is essential for vaccine design.Taken together,our results provided the current information about seasonal FLUV in Wuhan which form the basis for vaccine updating.

关键词： Seasonal influenza Molecular epidemiology Phylogenic analysis Vaccine effectiveness

An efficient strategy for establishing a model of sensorineural deafness in rats

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2015年第10期10卷 1683-1689页

作者： Long Ma Hai-jin Yi Fen-qian Yuan wei-wei guo Shi-ming Yang The Second Artillery General Hospital of Chinese PLA Department of Otolaryngology-Head & Neck Surgery Beijing Tiantan Hospital Capital Medical University Department of Head and Neck Surgery Jiangxi Cancer Hospital Department of Otolaryngology Head & Neck Surgery Institute of Otolaryngology Chinese PLA General Hospital

Ototoxic drugs can be used to produce a loss of cochlear hair cells to create animal models of deafness. However, to the best of our knowledge, there is no report on the establishment of a rat deafness model through the combined application of aminoglycosides and loop diuretics. The aim of this study was to use single or combined administration of furosemide and kanamycin sulfate to establish rat models of deafness. The rats received intravenous injections of different doses of furosemide and/or intramuscular injections of kanamycin sulfate. The auditory brainstem response was measured to determine the hearing threshold after drug application. Immunocytochemistry and confocal microscopy were performed to evaluate inner ear morphology. In the group receiving combined administration of furosemide and kanamycin, the auditory brainstem response threshold showed significant elevation 3 days after administration, higher than that produced by furosemide or kanamycin alone. The hair cells showed varying degrees of injury, from the apical turn to the basal turn of the cochlea and from the outer hair cells to the inner hair cells. The spiral ganglion cells maintained a normal morphology during the first week after the hair cells completely disappeared, and then gradually degenerated. After 2 months, the majority of spiral ganglion cells disappeared, but a few remained. These findings demonstrate that the combined administration of furosemide and kanamycin has a synergistic ototoxic effect, and that these drugs can produce hair cell loss and hearing loss in rats. These findings suggest that even in patients with severe deafness, electronic cochlear implants may partially restore hearing.

关键词： nerve regeneration sensorineural deafness kanamycin furosemide ototoxic drug spiral ganglion cells hair cell neural regeneration

Homogeneity analysis of Y-bearing 12Cr ferritic/martensitic steel fabricated by vacuum induction melting and casting

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research(International) 2020年第8期27卷 940-951页

作者： wei-wei guo Xiao-xin Zhang Ying-xue Chen Zhi-yuan Hong Qing-zhi Yan Institute of Nuclear Materials School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083China Advanced Energy Research Center Shenzhen UniversityShenzhen 518060GuangdongChina Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518060GuangdongChina

Advanced oxide metallurgy technique was adopted to produce 100-kg Y-bearing 12Cr ferritic/martensitic steel via vacuum induction melting and casting route. Subsequently, nine specimens at top, middle and bottom regions of the sheet were char-acterized to evaluate the homogeneity of chemical composition, microstructure and mechanical properties. The small vibra-tion of hardness (200–220 HBW), ultimate tensile strength (672–678 MPa), yield strength (468–480 MPa), total elongation (26.2%–30.5%) and Charpy energy at room temperature (98–133 J) and at ??40 ℃ (12–40 J) demonstrated that mechanical properties’ homogeneity of Y-bearing steel was acceptable although slight Y segregation and inhomogeneous microstructure occurred at the bottom. Furthermore, the effect of Y content on microstructure characteristics and mechanical properties was explained and the comparison of failure mechanism for the dual-phase steel between tensile test (i.e., quasi-static loading) and Charpy test (i.e., dynamic loading) was discussed in detail.

关键词： Y-bearing ferritic/martensitic steel Casting Homogeneity Chemical composition Microstructure characteristics Mechanical property

Unusual peroxidase activity of a myoglobin mutant with two distal histidines

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2012年第6期23卷 741-744页

作者： wei wei guo Dun Wan Li Fu Liao Ying Wu Lin School of Chemistry and Chemical Engineering University of South China Hengyang 421001 China State Key Laboratory of Coordination Chemistry Nanjing University Nanjing 210093 China

由在精子鲸鱼肌球素(Mb ) 保留本国的远侧的 His64，第二远侧的 histidine 被变异的 Leu29 在 Mb 设计到 His29。L29H Mb 的结果的异种与野类型 Mb 的相比与积极 cooperativity 展出一项不平常的提高的 peroxidase 活动。有二合作远侧的... 详细信息

由在精子鲸鱼肌球素(Mb ) 保留本国的远侧的 His64，第二远侧的 histidine 被变异的 Leu29 在 Mb 设计到 His29。L29H Mb 的结果的异种与野类型 Mb 的相比与积极 cooperativity 展出一项不平常的提高的 peroxidase 活动。有二合作远侧的 histidines 的新酶没在本国的 peroxidase 被发现，它强调合理蛋白质设计的创造。

关键词：过氧化物酶肌红蛋白组氨酸突变体远端活性抹香鲸野生型

Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua Li weiwei guo Shuolong Yuan Liangwei Xu Chang Lin Xi Shi wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory dysfunction.However,there are currently no effective pharmacological interventions for patients with noise-induced hearing loss.Here,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory mechanisms.We established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 hours.We found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with tranylcypromine.The noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with saline.These findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell loss.Additionally,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy pathway.Tranylcypromine treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)production.In conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing function.These findings suggest that inhibiting lysine-specific demethylase 1 is a potential therapeutic strategy for preventing h

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine