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基于UHPLC-Q-Exactive Orbitrap-MS的三种基原溪黄草及其干预小鼠肝纤维化的血清化学研究

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中南药学 2024年第2期22卷 296-301页

作者：覃萍苏阳静陈永苗郑瑞瑶钟佳妮叶晓燕葛跃伟陈阿丽广东药科大学新药研发中心广东省药物新剂型重点实验室广州510006 广东药科大学附属第一医院广州510062 广东药科大学中药学院广州510006

目的应用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UHPLC-Q-Exactive Orbitrap-MS)技术研究三种基原溪黄草及其在肝纤维化小鼠口服给药后的血清化学成分。方法采用Shim-pack GIST-HP C_(18)色谱柱(2.1 mm×150 mm,3μm);流动相为0.1... 详细信息

目的应用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UHPLC-Q-Exactive Orbitrap-MS)技术研究三种基原溪黄草及其在肝纤维化小鼠口服给药后的血清化学成分。方法采用Shim-pack GIST-HP C_(18)色谱柱(2.1 mm×150 mm,3μm);流动相为0.1%甲酸水(A)-甲醇(B),梯度洗脱,流速0.3 mL·min^(-1),柱温30℃。通过结合高分辨质谱数据及质谱裂解规律,对三种基原溪黄草药材样本及其给药小鼠血清的化学成分进行表征。结果在三种基原溪黄草药材中鉴定出40个化合物,主要包括二萜类、黄酮类、酚酸类化合物。在小鼠血清中鉴定出2.入血成分以及2.可能的代谢产物。结论本研究初步鉴定了三种基原溪黄草在四氯化碳致肝纤维化小鼠中的入血成分及部分代谢产物,为其用于治疗肝纤维化的药效物质基础提供了理论参考。

关键词：溪黄草肝纤维化超高效液相色谱-四极杆-静电场轨道阱串联质谱

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点接触边界润滑吸附膜计算模型

摩擦学学报 2008年第3期28卷 240-243页

作者：王慰祖黄平华南农业大学工程学院广东广州510642 华南理工大学机械工程学院广东广州510640

根据2.假设提出1种边界润滑的计算模型,通过吸附热理论对公式进行修正,利用自行设计的球-盘摩擦磨损试验装置,在汉硬脂酸的石油醚溶液中进行试验,所用45#钢盘的表面粗糙度分别为0.2.m和0.4μm,对理论计算数据和实验数据进行对比,并分... 详细信息

根据2.假设提出1种边界润滑的计算模型,通过吸附热理论对公式进行修正,利用自行设计的球-盘摩擦磨损试验装置,在汉硬脂酸的石油醚溶液中进行试验,所用45#钢盘的表面粗糙度分别为0.2.m和0.4μm,对理论计算数据和实验数据进行对比,并分析温度和速度等参数对摩擦系数的影响.结果表明,计算结果和实验数据的变化趋势吻合,摩擦系数随着温度增加而增大,随速度增加略有减小.

关键词：边界润滑分子吸附膜摩擦系数膜压力

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Journal of Genetics and Genomics 2017年第10期44卷 503-506页

作者： Xin Huang Lu Yang Feng-Feng Cai Yufei Wang ping Chen Jiangsheng Mi Chenghua Yu Jianghua Lai Xiaojun Zhang Shuguang wei Wen Cui Su Chen School of Forensic Sciences Xi'an Jiaotong University Health Science Center Xi'an 710061 China Center for Translational Medicine at The First Affiliated Hospital Xi'an Jiaotong University Health Science Center Xi'an 710061 China Department of Breast Surgery of Yangpu Hospital Tongji University Shanghai 200092 China School of Life Sciences and Technology Tongji University Department of Science and Education People's Hospital of Zunhua Tangshan 064200 China School of Forensics and Laboratory Medicine jining Medical University Jining 272067 China

Autophagy is an evolutionarily conserved lysosome-mediated catabolic process（Klionsky,2.07）.Autophagy is believed to be essential for cell survival,especially when cells were exposed to stresses,such as nutrient sta... 详细信息

关键词： ATG RNF Autophagy-related protein ATG5 regulates histone H2. mono-ubiquitylation by translational control of RNF2.

Gallstone recurrence after successful percutaneous cholecystolithotomy:a 10-year follow-up of 439 cases

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Hepatobiliary & Pancreatic Diseases International 2007年第2期6卷 199-203页

作者： Zou, Yi-ping Du, Ji-Dong Li, wei-Min Xiao, Yin-Qi Xu, Hong-Bin Zheng, Fang Huang, Hui Liu, Hao-Run Li, Hu-Cheng Beijing PLA 309 Hosp Dept Hepatobiliary Surg Beijing 100091 Peoples R China

BACKGROUND: Stone recurrence is a major problem in the medication of gallstones with gallbladder preservation. The aim of this study was to determine the long-term recurrence rate of gallstones and the clinical outcome after successful percutaneous cholecystolithotomy (PCCL) treatment, and to investigate the possible risk factors for gallstone recurrence. METHODS: After successful PCCL for gallstones, 439 patients were followed up during a 10-year period. The long-term gallstone recurrence rate and clinical outcome were evaluated. Risk factors associated with stone recurrence were identified. RESULTS: Gallstone recurrence was detected in 182.of 439 PCCL patients, giving an overall recurrence rate of 41.46%. The cumulative gallstone recurrence rate for each of the 10 post-operative years was 9.57%, 18.91%, 2..33%, 34.14%, 37.59%, 39.86%, 41.90%, 42.73%, 42.85%, and 43.2.%, respectively. Among these recurrent patients, 94 were asymptomatic, 80 suffered from nonspecific upper gastrointestinal symptoms and 8 suffered from abdominal pain or biliary colic. Thirty-eight of the 182.patients were retreated with cholecystectomy. The risk factors for stone recurrence included a family history of gallstones, preference for fatty food, accompanying liver disease, multiple stones and poor gallbladder function pre-PCCL. CONCLUSIONS: In this study, the overall recurrence rate of gallstone was 41.46% during a 10-year period. The highest frequency of gallstone recurrence was during the 5th to 6th postoperative years and then continued to slowly increase. Risk factors for stone recurrence varied. We suggest that the use of PCCL in patients with gallstones should be considered carefully because of stone recurrence.

关键词： gallstone percutaneous cholecystolithotomy recurrence risk factors

Construction, expression and characterization of the engineered antibody against tumor surface antigen, p185c-erbB-2

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Cell Research 2003年第1期13卷 35-48页

作者： LIAN SHENG CHENG, AI ping LIU, JIA HONG YANG, YAN QIU DONG, LIANG wei LI, JING WANG, CHAO CHEN WANG, JING LIUSchool of Life Science, University of Science and Technology of China, Hefei 2.002., China School of Life Science University of Science and Technology of China Hefei China

The c-erbB-2.proto-oncogene encodes a 185kDa protein p!85, which belongs to epidermal growth factor receptor family. Amplification of this gene has been shown to correlate with poor clinical prognosis for certain cancer patients. The monoclonal antibody A2. which directed against p185 specifically inhibits proliferation of tumor cells overexpressing p185, hence allows it to be a candidate for targeted therapy. In order to overcome several drawbacks of murine MAb, we cloned its VH and VL genes and constructed the single-chain Fv (scFv) through a peptide linker. The recombinant scFvA2. was expressed in Escherichia coli and purified by the affinity column. Subsequently it was characterized by ELISA, Western blot, cell immunohistochemistry and FACS. All these assays showed the binding activity to extracellular domain (ECD) of p!85. Based on those properties of scFvA2., we further constructed the scFv-Fc fusion molecule with a homodimer form and the recombinant product was expressed in mammalian cells. In a

关键词： p185 c-erbB-2 scFv scFv-Fc.

Motile parameters of cell migration in anisotropic environment derived by speed power spectrum fitting with double exponential decay

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Frontiers of physics 2020年第1期15卷 51-61页

作者： Yan-ping Liu Xiang Li Jing Qu Xue-Juan Gao Qing-Zu He Li-Yu Liu Ru-Chuan Liu Jian-wei Shuai Department of Physics Xiamen UniversityXiamen 361005China State Key Laboratory of Cellular Stress Biology.Innovation Center for Cell Signaling Network.Xiamen University Xiamen 361102China College of Physics Chongqing UniversityChongqing 401331China National Institute for Big Data in Healthcare at Xiamen University Xiamen 361102China

Cell migration through anisotropic microenvironment is critical to a wide variety of physiological and pathological ***,adequate analytical tools to derive motile parameters to characterize the anisotropic migration are ***,we proposed a method to obtain the four motile parameters of migration cells based on the anisotropic persistent random walk model which is described by two persistence times and two migration speeds at perpendicular *** key process is to calculate the velocity power spectra of cell migration along intrinsically perpendicular directions respectively,then to apply maximum likelihood estimation to derive the motile parameters from the power spectra fitting with double exponential *** simulation results show that the averaged persistence times and the corrected migration speeds can be good estimations for motile parameters of cell migration.

关键词： cell migration heterogeneity power spectrum random walk Langevin equation

Temporal–Spatial Patterns of Relative Humidity and the Urban Dryness Island Effect in Beijing City

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Temporal–Spatial Patterns of Relative Humidity and the Urba...

Comparison of Community Detection Algorithms on Contracts Networks

第35届中国气象学会年会

作者： ping YANG GUOYU REN wei Hou China Metrological Administration(CMA) Training Center Institute of Urban Meteorology CMA Laboratory for Climate Studies National Climate CenterCMA

Hourly datasets obtained by automatic weather stations in Beijing are developed and employed to analyze the spatial and temporal characteristics of relative humidity(RH)and urban dryness island intensity(UDII)over built-up areas.A total of 36 stations inside the sixth ring road(RR)are considered as urban sites,while six stations in

关键词： Spatial Patterns of Relative Humidity and the Urban Dryness Island Effect in Beijing City Temporal

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Comparison of Community Detection Algorithms on Contracts Ne...

第40届中国控制会议

作者： Peichen Xiong wei ping Hao Chen Information Institute Zhejiang University of Finance & Economics Dongfang College School of Information Engineering College of Science and Technology Ningbo University Ningbo University

Community discovery is an important research content in complex network analysis and an important tool for community analysis. It divides nodes into clusters according to the similarity of nodes or similarities in structure. This paper takes the EU public procurement contract network from 2.08 to 2.16 as an example, selects 6 countries with different data sizes,Austria, Cyprus, Germany, France, Hungary and Italy, analyzes the characteristics of the contract network and evaluates different community classification algorithms Performance. Taking the running speed and modularity as the evaluation criteria, comparing the Louvain algorithm and Girvan Newman algorithm the speed and accuracy of these 2.methods on different scale data sets, analyze the most suitable contract network community classification algorithm. Through analysis, the contract network is a sparse bipartite network, Louvain algorithm is a more suitable community detection method than Girvan Newman algorithm for contract networks. It has a fast running speed and high accuracy.

关键词： Social Network Analysis Community Detection Girvan Newman Algorithm Louvain Algorithm

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辛开苦降法治疗胃癌理论探析

中医肿瘤学杂志 2024年第3期6卷 83-88页

作者：汪学鹏郭昊铭师亮亮王帅哲陈亚萍杨永琴魏本君甘肃中医药大学中医临床学院甘肃兰州730000 甘肃省中医方药挖掘与创新转化重点实验室甘肃兰州730000

辛开苦降法是基于中药四气五味药性理论,以辛味药和苦味药并用,来达到脾胃升降恢复、气机斛旋平衡的作用。由于其可以恢复脾胃的升降功能及调整气的运行,故适用于多种脾胃系统疾病的治疗。近些年中医药治疗癌症具有显著疗效,胃癌的病机... 详细信息

辛开苦降法是基于中药四气五味药性理论,以辛味药和苦味药并用,来达到脾胃升降恢复、气机斛旋平衡的作用。由于其可以恢复脾胃的升降功能及调整气的运行,故适用于多种脾胃系统疾病的治疗。近些年中医药治疗癌症具有显著疗效,胃癌的病机与辛开苦降的理论相对应,本文尝试从辛开苦降法的学术源流、治疗胃癌的立法依据及临床应用等方面阐明辛开苦降法治疗胃癌的理论基础。

关键词：辛开苦降胃癌治法