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Expression of hepatitis B virus surface antigens induces defective gonad phenotypes in Caenorhabditis elegans

World Journal of Virology 2017年第1期6卷 17-25页

作者： Yi-Yin Chen Li-wei Lee wei-ning hong Szecheng J Lo Department of Biomedical Science College of Medicine Chang Gung University Graduate Institute of Biomedical Science College of Medicine Chang Gung University

AIM To test whether a simple animal, Caenorhabditis elegans(C. elegans), can be used as an alternative model to study the interaction between hepatitis B virus antigens(HBs Ag) and host factors. METHODS Three plasmids that were able to express the large, middle and small forms of HBs Ags(LHBs Ag, MHBs Ag, and SHBs Ag, respectively) driven by a ubiquitous promoter(fib-1) and three that were able to express SHBs Ag driven by different tissue-specific promoters were constructed and microinjected into worms. The brood size, egglaying rate, and gonad development of transgenic worms were analyzed using microscopy. Levels of m RNA related to endoplasmic reticulum stress, enpl-1, hsp-4, pdi-3 and xbp-1, were determined using reverse transcription polymerase reaction(RT-PCRs) in three lines of transgenic worms and dithiothreitol(DTT)-treated wild-type worms. RESULTS Severe defects in egg-laying, decreases in brood size, and gonad retardation were observed in transgenic worms expressing SHBs Ag whereas moderate defects were observed in transgenic worms expressing LHBs Ag and MHBs Ag. RT-PCR analysis revealed that enpl-1, hsp-4 and pdi-3 transcripts were significantly elevated in worms expressing LHBs Ag and MHBs Ag and in wild-type worms pretreated with DTT. By contrast, only pdi-3 was increased in worms expressing SHBs Ag. To further determine which tissue expressing SHBs Ag could induce gonad retardation, we substituted the fib-1 promoter with three tissue-specific promoters(myo-2 for the pharynx, est-1 for the intestines and mec-7 for the neurons) and generated corresponding transgenic animals. Moderate defective phenotypes were observed in worms expressing SHBs Ag in the pharynx and intestines but not in worms expressing SHBs Ag in the neurons, suggesting that the secreted SHBs Ag may trigger a cross-talk signal between the digestive track and the gonad resulting in defective phenotypes. CONCLUSION Ectopic expression of three forms of HBs Ag that causes recognizable pheno

关键词： Hepatitis B virus Caenorhabditis elegans Green fluorescence proteins Endoplasmic reticulum stress Gonad retardation Surface antigens

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Non-contact monitoring and analysis system for tunnel surrounding rock deformation of underground engineering

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中国有色金属学会会刊：英文版 2005年第S1期15卷 96-99页

作者： YANG Song-lin, WANG Bin, JI Sheng-yue, LIU wei-ning, SHI hong-yun (Beijing Jiaotong University, Beijing 100044, China) Beijing Jiaotong Univ Beijing 100044 Peoples R China

It is very important to monitor surrounding rock deformation in tunnel construction. The principle, function, development and application of the system composed of a total station and computer for monitoring and analyzing surrounding rock deformation were discussed. The new methods of two free station of 3D measurement and its mathematic adjustment mode were presented. The development of software for total station on-board and post for computer were also described. Without centering it and measuring its height, the total station controlled by the software on-board can fulfill the whole measurements to target points. Monitoring data can be processed by the post software and results of regression analysis, forecasting information of the tunnel surrounding rock deformation can be provided in time. The practical use shows that this system is practicable, highly accurate and efficient. It satisfies the needs of safety and information construction in tunnel construction of underground engineering.

关键词： tunnel construction deformation of surrounding rock total station non-contact monitoring data processing and analysis

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新收入准则对房地产行业的税务影响

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中小企业管理与科技 2021年第2期5卷 74-75页

作者：宁卫红昆明绿地春城置业有限公司昆明650011

财政部在2017年修订了新收入准则,这一举措,对企业和行业产生了非常大的影响。经过修订之后的新收入准则,不仅在会计信息的可比性方面得到了提升,同时对于收入确认时点和收入金额计量这两个方面也带来了一系列的税法和会计间差异。针对... 详细信息

财政部在2017年修订了新收入准则,这一举措,对企业和行业产生了非常大的影响。经过修订之后的新收入准则,不仅在会计信息的可比性方面得到了提升,同时对于收入确认时点和收入金额计量这两个方面也带来了一系列的税法和会计间差异。针对房地产行业比较普遍的业务场景,论文对新收入准则下产生的房地产行业的税会差异,还有在增值税、企业所得税以及土地增值税这三个方面产生的作用展开分析。

关键词：新收入准则房地产行业税务影响

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地舒单抗对绝经后骨质疏松性股骨颈骨折全髋关节置换术后股骨近端假体周围骨密度的影响

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中国骨伤 2023年第11期36卷 1041-1045页

作者：宁伟宏徐国柱王建伟湖州市长兴县中医院骨科浙江湖州313100

目的:研究地舒单抗对绝经后骨质疏松性股骨颈骨折患者全髋关节置换术后(total hip arthroplasty,THA)股骨近端假体周围骨密度的影响。方法:选取2020年10月至2021年10月绝经后女性骨质疏松性股骨颈骨折行THA术后54例,治疗组25例接受地舒... 详细信息

目的:研究地舒单抗对绝经后骨质疏松性股骨颈骨折患者全髋关节置换术后(total hip arthroplasty,THA)股骨近端假体周围骨密度的影响。方法:选取2020年10月至2021年10月绝经后女性骨质疏松性股骨颈骨折行THA术后54例,治疗组25例接受地舒单抗治疗,年龄(74.3±6.2)岁;对照组29例未接受地舒单抗治疗,年龄(75.2±4.8)岁。术后1周及3、6及12个月各个时间点,通过双能X线骨密度仪(DEXA型)测定股骨近端假体周围骨密度,并在不同时间点测量骨转换各项指标。结果:术后3、6及12个月对照组的抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRACP-5b)高于治疗组(P0.05)。两组术后3个月Gruen 1、7区比较,差异无统计学意义(P>0.05);术后6个月Gruen 1、7区和术后12个月Gruen 1、7区,治疗组骨密度均明显高于对照组(P0.05)。对照组术后6个月Gruen 1、7区,术后12个月Gruen 1、7区骨密度下降百分比明显高于治疗组(P<0.05)。提示在使用地舒单抗6个月后,即可降低骨密度丢失幅度,并且该效应可达至术后12个月。结论:绝经后骨质疏松性股骨颈骨折患者在THA术后,使用地舒单抗可减少股骨近端假体周围骨密度丢失,有效抑制骨吸收。

关键词：地舒单抗绝经后骨质疏松全髋关节置换术

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Clinical effects of phospholipase D2 in attenuating acute pancreatitis

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World Journal of Gastroenterology 2025年第2期31卷 52-60页

作者： Jin-wei Niu Guo-Chao Zhang Wu ning Hai-Bin Liu Hua Yang Chao-Feng Li Department of General Surgery China-Japan Friendship HospitalBeijing 100029China

BACKGROUND The objective of the current study was to elucidate the clinical mechanism through which phospholipase D2(PLD2)exerted a regulatory effect on neutrophil migra-tion,thereby alleviating the progression of acute *** To elucidate the clinical mechanism through which PLD2 exerted a regulatory effect on neutrophil migration,thereby alleviating the progression of acute *** The study involved 90 patients diagnosed with acute pancreatitis,admitted to our hospital between March 2020 and November 2022.A retrospective analysis was conducted,categorizing patients based on Ranson score severity into mild(n=25),moderate(n=30),and severe(n=35)*** data was collected for each *** blot analysis assessed PLD2 protein expression in patient ***-time reverse transcription polymerase chain reaction was used to evaluate the mRNA expression of chemokine receptors associated with neutrophil *** levels of inflammatory factors in patients were detected using enzyme-linked immunosorbent *** migration tests were conducted to compare migration of neutrophils across groups and analyze the influence of PLD2 on neutrophil *** Overall data analysis did not find significant differences between patient groups(P>0.05).The expression of PLD2 protein in the severe group was lower than that in the moderate and mild groups(P<0.05).The expression level of PLD2 in the moderate group was also lower than that in the mild group(P<0.05).The severity of acute pancreatitis is negatively correlated with PLD2 expression(r=-0.75,P=0.002).The mRNA levels of C-X-C chemokine receptor type 1,C-X-C chemokine receptor type 2,C-C chemokine receptor type 2,and C-C chemokine receptor type 5 in the severe group are significantly higher than those in the moderate and mild groups(P<0.05),and the expression levels in the moderate group are also higher than those in the mild group(P<0.05).The levels of C-reactive protein,tumo

关键词： Phospholipase D2 Neutrophil migration Acute pancreatitis Retrospective analysis Inflammatory response

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互感器校验装置综述

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电力系统保护与控制 2009年第10期37卷 131-135页

作者：宁伟红杨以涵李静张国庆于文斌华北电力大学电气与电子工程学院北京102206

互感器校验装置是用于检定电压互感器与电流互感器的专用设备,随着现代工业的发展,对互感器校验仪的要求也逐渐提升,互感器校验仪也随之不断发展。主要介绍了互感器的校验方法;概述了互感器校验仪从手动到自动,高智能化的发展,并根据其... 详细信息

互感器校验装置是用于检定电压互感器与电流互感器的专用设备,随着现代工业的发展,对互感器校验仪的要求也逐渐提升,互感器校验仪也随之不断发展。主要介绍了互感器的校验方法;概述了互感器校验仪从手动到自动,高智能化的发展,并根据其发展对互感器校验仪进行了分类,简要分析了各种取差、测差电路以及相关产品,指出互感器校验仪的最新发展方向。

关键词：互感器校验仪比差相角误差虚拟仪器

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电能计量系统发展综述

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电力系统保护与控制 2009年第11期37卷 130-134页

作者：李静杨以涵于文斌张国庆宁伟红华北电力大学电气与电子工程学院北京102206

基于互感器、电能表和二次接口等方面回顾了电能计量装置的发展历程和原理,对21世纪现代电能计量装置的发展趋势进行了展望,指出数字化、智能化、标准化、系统化和网络化是现代电能计量系统发展的必然趋势。另外,还介绍了几个新型互感器... 详细信息

基于互感器、电能表和二次接口等方面回顾了电能计量装置的发展历程和原理,对21世纪现代电能计量装置的发展趋势进行了展望,指出数字化、智能化、标准化、系统化和网络化是现代电能计量系统发展的必然趋势。另外,还介绍了几个新型互感器(Rogowski光电式电流互感器、法拉第磁光效应互感器和光学电压互感器),特殊功用的新型电能表(单相电子式复费率电能表、三相预付费电表和数字式电能表)和几种常用电能计量芯片。

关键词：电能计量互感器计量芯片

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Cauda equina syndrome with urinary retention as a postoperative complication of lumbar spine surgery:A case report

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World Journal of Clinical Cases 2025年第10期13卷 40-45页

作者： Kai-Wu Yang wei-hong Lai Da-wei Huang Department of Urology Chiayi Christian HospitalChiayi 600566Taiwan Department of Neurosurgery Chiayi Christian HospitalChiayi 600566Taiwan

BACKGROUND Cauda equina syndrome(CES)is characterized by a group of symptoms that may be caused by inflammation,spinal cord compression,venous congestion,or *** syndrome is commonly an indication for surgical intervention but has not been determined as a postoperative complication following surgery for lumbar spine *** SUMMARY To report the case of a 54-year-old male patient who had CES following spinal surgery,with no obvious compression lesions found during re-exploration,suggesting that vascular insufficiency may have contributed to the ***,a series of urodynamic studies on bladder recovery patterns in such complications have also been *** Postoperative CES requires urgent imaging and exploration to rule out compression;noncompressive cases,including vascular insufficiency may performed conservative management.

关键词： Cauda equina syndrome Lumbar spine surgery Urinary retention Urodynamic study Postoperative complication Case report

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北部湾经济区建设人才需求与高职院校专业设置研究

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成人教育 2010年第12期30卷 23-24页

作者：韦红宁陈丽金韩志刚广西工业职业技术学院广西南宁530001

广西高职院校应退出、缩减或整合部分设点过多、发展过热的专业;应结合所在城市主要的区域发展功能定位和自身资源,增设支撑北部湾经济区九大重点发展产业的新专业;应通过开办"目录外"专业或给老专业增设专业方向来培养北部湾经济区紧... 详细信息

广西高职院校应退出、缩减或整合部分设点过多、发展过热的专业;应结合所在城市主要的区域发展功能定位和自身资源,增设支撑北部湾经济区九大重点发展产业的新专业;应通过开办"目录外"专业或给老专业增设专业方向来培养北部湾经济区紧缺的复合型人才。

关键词：北部湾经济区高职院校专业设置策略分析

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The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： Shengyang Zhou Ting Li wei Zhang Jian Wu Hui hong wei Quan Xinyu Qiao Chun Cui Chenmeng Qiao weijiang Zhao Yanqin Shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 STING type I interferon

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